Abstract:
:We have previously isolated 3T3 cell variants unable to respond to specific mitogens. In this report we analyze the dominant and/or recessive nature of these variants. Two independently isolated EGF nonproliferative variants are unable to bind EGF. Hybrids between 3T3R5 cells (thymidine kinase deficient, ouabain-resistant) and these variants express EGF receptors; the "EGF receptorless" phenotype of these variants is recessive. Hybrids between these two variants do not bind EGF; they are defective in a common, non-complementing function. A TPA nonproliferative 3T3 variant is also recessive; hybrids with 3T3R5 mount a mitogenic response to TPA. In contrast a fourth variant, which can neither bind labeled EGF nor respond to TPA, is dominant for both characteristics. Hybrids between this latter variant and 3T3R5 can neither bind EGF nor mount a mitogenic response to TPA.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Terwilliger E,Herschman HRdoi
10.1002/jcp.1041230305subject
Has Abstractpub_date
1985-06-01 00:00:00pages
321-5issue
3eissn
0021-9541issn
1097-4652journal_volume
123pub_type
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