Abstract：
:In order to study the generation, factors controlling endogenous folate pools, and their functional importance, Friend erythroleukemia cells were grown in media containing 100; 1,000; and 10,000 ng/ml of tritiated pteroylglutamic acid (3H)PteGlu1 and then studied in unlabeled media with varying amounts of PteGlu1. The intracellular folate pool was directly proportional to the PteGlu1 in which the cells were incubated. At equilibrium, greater than 95% of the labeled intracellular folate pool chromatographed as polyglutamyl folate, regardless of the exogenous folate concentration. The functional importance of the intracellular folate pool was studied by varying the endogenous pool and the exogenous (media) supply. The ability of the cells to replicate in the absence of exogenous folate was directly proportional to the intracellular polyglutamyl folate pool. The maximal rate of replication, however, required exogenous PteGlu1 in addition. The cell doubling time was the most important determinant of intracellular folate turnover; changes in the intracellular pool size and the extracellular folate concentration had no effect on the turnover time. In a rapidly proliferating tissue, the onset of functional folate deficiency will be determined by dilution of intracellular polyglutamates among progeny until a critical level is reached.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Steinberg SE,Fonda S,Campbell CL,Hillman RSdoi
10.1002/jcp.1041140216subject
Has Abstractpub_date
1983-02-01 00:00:00pages
252-6issue
2eissn
0021-9541issn
1097-4652journal_volume
114pub_type
杂志文章abstract：:The great importance of long noncoding RNAs (lncRNAs) has been acknowledged in tumorigenesis gradually. LncRNA LINC01857 is a novel lncRNA and has been reported to promote breast cancer progression. However, the biological roles of LINC01857 in glioma are not explored. In the present research, LINC01857 levels were fo...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28763
更新日期：2019-12-01 00:00:00
abstract：:To evaluate the regulation of endothelial cell Cu,Zn-SOD, we have exposed bovine pulmonary artery endothelial cells in culture to hyperoxia and hypoxia, second messengers or related agonists, hormones, free radical generating systems, endotoxin, and cytokines and have measured Cu,Zn-SOD protein of these cells by an EL...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041530308
更新日期：1992-12-01 00:00:00
abstract：:Human polyomaviruses, which include JC virus (JCV) and BK virus (BKV), as well as the simian virus 40 (SV40), have been associated with human tumors and have been shown to be highly tumorigenic in experimental animal models. Although the mechanism by which JCV induces tumorigenesis is not entirely clear, earlier studi...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21005
更新日期：2007-07-01 00:00:00
abstract：:Tumor ischemia participates in angiogenesis and cancer progression through cellular responses to hypoxia and nutrient deprivation. However, the contribution of amino acids limitation to this process remains poorly understood. Using serum-free cell culture conditions, we tested the impact of L-glutamine deprivation on ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21044
更新日期：2007-08-01 00:00:00
abstract：:Transforming growth factor-beta (TGF-beta) signaling in endothelial cells is able to modulate angiogenesis and vascular remodeling, although the underlying molecular mechanisms remain poorly understood. Endoglin and ALK-1 are components of the TGF-beta receptor complex, predominantly expressed in endothelial cells, an...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20311
更新日期：2005-08-01 00:00:00
abstract：:Fibroblastic and epithelioid clones have been isolated from the normal rat kidney line, NRK. These clones were studied for their ability to bind epidermal growth factor (EGF), susceptibility to transformation by mouse sarcoma virus (MSV), and alteration in EGF binding upon sarcoma virus transformation. The epithelioid...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040940311
更新日期：1978-03-01 00:00:00
abstract：:The synthesis and degradation of pectoralis myosin have been investigated in chickens 15-23 days after hatching. An essentially nonreutilizable tracer amino acid was used to demonstrate differences in the rates of synthesis and degradation of myosin isolated from normal and hypertrophied, cystrophic breast tissue. The...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040860406
更新日期：1975-10-01 00:00:00
abstract：:Suramin is an anti-cancer drug which induces the differentiation of the human colon cancer clone HT29-D4. Yet chronic suramin treatment of these cells eventually leads to a marked disturbance of the lysosomal system, which consists in an accumulation of hypertrophied autophagic vacuoles and the occurrence of lamellate...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041500122
更新日期：1992-01-01 00:00:00
abstract：:Tenascin-C, a six-armed extracellular matrix glycoprotein, is expressed in a temporally and spatially restricted pattern during carcinogenesis and invasion or metastasis of carcinoma cells in association with stromal-epithelial interactions. The human epidermoid carcinoma-derived cell lines, A431 and HEp-2, which do n...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041650104
更新日期：1995-10-01 00:00:00
abstract：:Cancer incidences are growing and cause millions of deaths worldwide. Cancer therapy is one of the most important challenges in medicine. Improving therapeutic outcomes from cancer therapy is necessary for increasing patients' survival and quality of life. Adjuvant therapy using various types of antibodies or immunomo...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.28122
更新日期：2019-08-01 00:00:00
abstract：:Retinoic acid inhibits proliferation of hormone-dependent, but not hormone-independent breast cancer cells. Retinoic acid-induced changes in cellular proliferation and differentiation are associated with disturbances in growth factor signaling and frequently with changes in protein kinase C expression. PKC delta, epsi...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199709)172:3<306::AID-JCP4
更新日期：1997-09-01 00:00:00
abstract：:The purpose of this study was to characterize the direct effects of uncarboxylated osteocalcin (ucOCN) on vascular cell biology in vitro, to assess its potential function in pathophysiological conditions such as atherosclerosis. Human aortic endothelial cells (HAECs) and smooth muscle cells (HASMCs) were treated with ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28707
更新日期：2019-11-01 00:00:00
abstract：:Gene therapy using biocompatible cationic liposomes is amongst promising approaches that decreases death from cancers. Here an invasive multidrug resistant cell model has been developed by lentiviral transfection. In parallel phospholipids have been covalently conjugated to TAT, MMP2, and Herceptin. The functional lip...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28683
更新日期：2019-11-01 00:00:00
abstract：:Chronic exposure of pancreatic beta-cells to supraphysiologic glucose causes adverse beta-cell dysfunction. Thus, the present study was aimed to investigate the hypothesis that oral administration of resveratrol attenuates hyperglycemia, proinflammatory cytokines and antioxidant competence and protects beta-cell ultra...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.22138
更新日期：2010-08-01 00:00:00
abstract：:A serum factor which enhances the growth/viability of SV40 transformed 3T3 mouse fibroblasts, but not untransformed 3T3 cells in tissue culture has been partially purified from calf serum. The purification, which involves acidification to pH 2, chromatography on Sephadex G-100 at pH 2 followed by either Sephadex G-25 ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040960203
更新日期：1978-08-01 00:00:00
abstract：:As a typical hibernator, the Daurian ground squirrel (Spermophilus dauricus) spends considerable time in a state of reduced activity with prolonged fasting. Despite this, they experience little muscle atrophy and have thus become an interesting anti-disuse muscle atrophy model. The IKKβ/NF-κB signaling pathway is sign...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.26751
更新日期：2018-11-01 00:00:00
abstract：:Activation of T-lymphocytes by antigen, mitogenic lectins, or antibodies against the T-cell receptor complex, particularly in the presence of IL1, induces the secretion of the T-cell growth factor IL2. IL2 then has a major role in regulating the duration and magnitude of the immune response. Interaction of antigen, an...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041410212
更新日期：1989-11-01 00:00:00
abstract：:The matricellular protein periostin is strongly expressed in collagen-rich connective tissues such as periodontal ligaments (PDLs), skeletal muscle, adipose tissue, tendons, skin, and bone. It is prominent in tumorigenesis, angiogenesis, and cardiac repair. It is localized in the periosteum and PDL, where it is seen i...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.24407
更新日期：2014-01-01 00:00:00
abstract：:We investigated gene expression profiles of vascular endothelial growth factor (VEGF) and pigment epithelium-derived factor (PEDF) in differentiated and non-differentiated retinal pigment epithelial (RPE) cells during oxidative stress. Human RPE cells were grown in culture on laminin-coated flasks to obtain differenti...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.10026
更新日期：2001-12-01 00:00:00
abstract：:Zinc finger E-box binding homeobox 1 (ZEB1), an important transcription factor belonging to the ZEB family, plays a crucial role in regulating gene expression required for both normal physiological and pathological processes. Accumulating evidence has shown that ZEB1 participates in the initiation and progression of a...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.30177
更新日期：2020-12-04 00:00:00
abstract：:Hyaluronan (HA) accumulates in vascular disease but its functional role is not fully understood. To investigate the impact of HA enriched extracellular matrices (ECM) on cell phenotype, arterial smooth muscle cells (ASMCs) were transduced with retroviral constructs (LXSN) encoding murine has-1, has-2, and has-3. HA sy...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20468
更新日期：2006-02-01 00:00:00
abstract：:We have previously observed major differences in the phosphorylation of membrane proteins in sparse, proliferating versus confluent, quiescent pig aortic endothelial cells (EC) (Kazlauskas and DiCorleto, 1987). In the present study we examined whether EC growth state can influence the activity of a specific phosphoryl...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041360306
更新日期：1988-09-01 00:00:00
abstract：:Prkdc encodes for the catalytic subunit of the DNA-dependent protein kinase (DNA-PKcs) playing a key role in nonhomologous end joining pathway during DNA double-strand break repair and also influencing the homologous recombination (HR) repair system by phosphorylation of proteins involved in HR. In addition, Prkdc has...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29295
更新日期：2020-04-01 00:00:00
abstract：:Breast cancer, as the most common cancer in women which affects patients both mentally and physically, requires great attention in many areas and many levels as this cancer is known to be multifactorial. Single-stranded molecules called microRNAs with near 22 nucleotides are seen to act in central dogma of molecular b...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.29966
更新日期：2021-03-01 00:00:00
abstract：:Optical tweezers were used to scan individual Chronic Myelogenous Leukemia cells to determine if the cell death depends on the scanning conditions. Although increasing the scanning frequency or amplitude means greater force applied to the cells, their effects on cell death are not a simple increasing trend, as observe...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.24369
更新日期：2013-10-01 00:00:00
abstract：:The physiological approach suggests that an environment associating the mesenchymal stromal cells (MSC) and low O(2) concentration would be most favorable for the maintenance of hematopoietic stem cells (HSCs) in course of ex vivo expansion of hematopoietic grafts. To test this hypothesis, we performed a co-culture of...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.23019
更新日期：2012-06-01 00:00:00
abstract：:We have recently described a novel nuclear antigen, AF-2, which is related to cell cycle dependent alterations of chromatin structure. We show by two parameter flow cytometry on a cell by cell basis that the antigen is accessible to specific monoclonal antibodies only in mitotic and postmitotic early G1-phase cells. T...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041490328
更新日期：1991-12-01 00:00:00
abstract：:Subcellular localization of the large multicatalytic protease complexes called proteasomes, which have been found in soluble fractions of various cells, was examined by biochemical, immunological, and immunohistological methods. Rat liver nuclei, purified by two different procedures, showed high activities for degradi...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041390107
更新日期：1989-04-01 00:00:00
abstract：:Although it is well established that epidermal growth factor receptors (EGFRs) are asymmetrically expressed at the basolateral plasma membrane in polarized epithelial cells, how this process is regulated is not known. The purpose of this study was to address the mechanism of directed EGFR basolateral sorting using the...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041620316
更新日期：1995-03-01 00:00:00
abstract：:Papillary thyroid carcinoma (PTC) is a common malignancy in thyroid tissue. However, the molecular mechanism of PTC tumor progression remains unknown. The hedgehog (Hh) pathway is thought to play a key role during PTC development. Here we investigate the effects of glioma-associated oncogene protein-2 (Gli2), an impor...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28399
更新日期：2019-08-01 00:00:00
