Abstract:
:We have recently described a novel nuclear antigen, AF-2, which is related to cell cycle dependent alterations of chromatin structure. We show by two parameter flow cytometry on a cell by cell basis that the antigen is accessible to specific monoclonal antibodies only in mitotic and postmitotic early G1-phase cells. The evaluation of nuclease susceptibility and AF-2 antigen accessibility reveals different subcompartments of the G1-phase of the cell cycle with distinct chromatin conformations. Digestion with DNase I seems to alter the chromatin structure according to concentration and this is reflected by an increase of the antigen accessibility. Chromatin in the more condensed early G1-phase is specifically digested by lower concentrations of the enzyme than chromatin in later stages of interphase. Chromatin from cells in the late-G1, S-, and G2-phases shows a higher relative resistance to DNase I and a reduced accessibility of the AF-2 antigen to monoclonal antibodies. Nuclease S1 has a similar effect on chromatin topology, as revealed by the reaction with anti-AF-2 antibodies, without digestion of detectable amounts of DNA. The antigen becomes available to the antibodies in almost all cells by digestion with high concentrations of DNase I or Nuclease S1.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Pfeffer U,Di Vinci A,Geido E,Vidali G,Giaretti Wdoi
10.1002/jcp.1041490328subject
Has Abstractpub_date
1991-12-01 00:00:00pages
567-74issue
3eissn
0021-9541issn
1097-4652journal_volume
149pub_type
杂志文章abstract::Hyperhomocysteinemia (HHcy) is associated with atherosclerotic events involving the modulation of arachidonic acid (AA) metabolism and the activation of matrix metalloproteinase-9 (MMP-9). Cytochrome P450 (CYP) epoxygenase-2J2 (CYP2J2) is abundant in the heart endothelium, and its AA metabolites epoxyeicosatrienoic ac...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21356
更新日期:2008-06-01 00:00:00
abstract::Emerging evidence have discovered that circular RNAs (circRNAs) may serve as diagnostic or tumor promising biomarkers. This study aimed to investigate how circular RNA ADAMTS14 (circADAMTS14) regulates microRNA-572/ regulator of calcineurin 1(miR-572/ RCAN1) in hepatocellular carcinoma (HCC). The expression profiles o...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26764
更新日期:2019-03-01 00:00:00
abstract::Recently, ATP has gained much interest as an extracellular messenger involved in the communication of calcium signals between cells. The mechanism of ATP release is, however, still a matter of debate. In the present study we investigated the possible contribution of connexin hemichannels or ion channels in the release...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10365
更新日期:2003-11-01 00:00:00
abstract::Prkdc encodes for the catalytic subunit of the DNA-dependent protein kinase (DNA-PKcs) playing a key role in nonhomologous end joining pathway during DNA double-strand break repair and also influencing the homologous recombination (HR) repair system by phosphorylation of proteins involved in HR. In addition, Prkdc has...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29295
更新日期:2020-04-01 00:00:00
abstract::We have analyzed the expression of Na,K-ATPase alpha subunit isoforms in the transporting ciliary processes of the human eye and in cultured cells derived from non-pigmented (NPE) and pigmented (PE) ciliary epithelium. Northern hybridization analysis shows that the mRNAs encoding all the three distinct forms of Na,K-A...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041410203
更新日期:1989-11-01 00:00:00
abstract::Quercetin has potentially beneficial effects on disease prevention, including cancer. An intriguing issue regarding the mechanisms of action of quercetin is the ability of this drug to modulate estrogen receptor (ER) activities. In a previous study, we demonstrated that quercetin elicited apoptosis through an ERα-depe...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22917
更新日期:2012-05-01 00:00:00
abstract::We observed previously that each of seven cancer progression inhibitors suppresses the mRNA expression of some matrix metalloproteinases (MMPs), but stimulates that of others, in breast cancer cells. In the present study we tested the effect of overexpressing other cancer modulators on MMP expression. The MMPs tested ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22694
更新日期:2011-12-01 00:00:00
abstract::Adult bone marrow mesenchymal stromal cells (BMSCs) can easily be differentiated into a variety of cells. In vivo transplantation of BMSCs-differentiated cells has had limited success, suggesting that these cells may not be fully compatible with the cells they are intended to replace in vivo. We investigated the struc...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25177
更新日期:2016-04-01 00:00:00
abstract::Insulin-like growth factor-I (IGF-I) promotes bone formation by stimulating proliferation and differentiation of osteoblasts. Bone sialoprotein (BSP), is thought to function in the initial mineralization of bone, is selectively expressed by differentiated osteoblast. To determine the molecular mechanism of IGF-I regul...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20664
更新日期:2006-08-01 00:00:00
abstract::Human fibroblasts derived from newborn foreskin and designated CF-3 were assayed for heat labile glucose-6-phosphate dehydrogenase (G6PD) when they had grown to confluency, as well as when they were arrested in an essentially nonmitotic state. Under both culture conditions there was an increase in heat labile G6PD (up...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040930108
更新日期:1977-10-01 00:00:00
abstract::Autophagy is an evolutionarily conserved intracellular process and is considered one of the main catabolism pathways. In the process of autophagy, cells are digested nonselectively or selectively to recover nutrients and energy, so it is regarded as an antiaging process. In addition to the essential role of autophagy ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.30111
更新日期:2021-01-16 00:00:00
abstract::Mouse keratinocytes are induced to differentiate in vitro by elevating the level of extracellular calcium from 0.05 mM, where keratinocytes express a basal cell phenotype, to greater than 0.10 mM, where they express the differentiated phenotype. This process has been associated with a rapid, sustained increase in inos...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041480113
更新日期:1991-07-01 00:00:00
abstract::The insulin-like growth factors (IGFs) are potent mitogens for malignant cell proliferation. The majority of secreted IGFs are bound to specific IGF-binding proteins (IGFBPs) that are secreted by a large number of cells. These proteins may either inhibit or enhance IGF actions. Breast carcinoma cells secrete a variety...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041580110
更新日期:1994-01-01 00:00:00
abstract::Incubation of primary cultures of parenchymal hepatocytes in a conditioned medium (CM), collected over the first 3 h of serum-free rat hepatocyte culture (CM(0-3)), induces a time dependent increase of the frequency of apoptotic cells which is accompanied by prominent changes of cell morphology. Short-term treatment w...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10439
更新日期:2004-03-01 00:00:00
abstract::Our objective was to establish the existence of intercellular communication through gap junctions in synovial lining cells and in primary and passaged cultures of human synovial cells. Communication between cells was assessed using the nystatin perforated-patch method, fluorescent dye transfer, immunochemistry, transm...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(200007)184:1<110::AID-JCP1
更新日期:2000-07-01 00:00:00
abstract::Estrogens mediate gene transcription and signaling of numerous cellular processes in a variety of tissues, including the bone, breast, and endometrium, through binding and activation of estrogen receptors (ERs). Estrogen-mediated ER agonist activity has shown benefit in conditions related to estrogen deficiency in wom...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.24324
更新日期:2013-07-01 00:00:00
abstract::Neuropathic pain (NP) may cause serious brain diseases, but the genes associated with the metabolic pathway and transcript factors of NP remain unclear. This study is aimed to identify the therapy target genes for NP and to investigate the metabolic pathways and transcript factors associated with NP. The differentiall...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27413
更新日期:2019-05-01 00:00:00
abstract::Fibronectin is a component of the extracellular matrix of developing microvessels whose role in angiogenesis is poorly understood. This study evaluated the effect of plasma fibronectin on angiogenesis in serum-free collagen gel culture of rat aorta. Aortic explants embedded in collagen gels generated microvascular out...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041540325
更新日期:1993-03-01 00:00:00
abstract::The role of platelet-derived growth factor (PDGF) in the control of smooth muscle cell (SMC) differentiation was explored in vitro by examining its effects on expression of the smooth muscle (SM) specific contractile protein SM alpha actin in cultured rat aortic SMC. Quiescent, postconfluent SMC express maximal levels...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041420325
更新日期:1990-03-01 00:00:00
abstract::Osteoblasts are terminally differentiated cells with mesenchymal origins, known to possess pivotal roles in sustaining bone microstructure and homeostasis. These cells are implicated in the pathophysiology of various bone disorders, especially osteoporosis. Over the last few decades, strategies to impede bone resorpti...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.27582
更新日期:2019-06-01 00:00:00
abstract::Fibrosis is a common pathological condition associated with abnormal repair after tissue injury. However, the etiology and molecular mechanisms of fibrosis are still not well-understood. Tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) belongs to the TNF superfamily and acts by binding to its recepto...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.30089
更新日期:2020-09-30 00:00:00
abstract::Tobacco smoking is associated with many diseases. Addiction is of the most notorious tobacco-related syndrome and is mainly attributed to nicotine. In this study, we employed Caenorhabditis elegans as a biological model to systemically investigate the effect of chronic nicotine exposure on microRNA (miRNA) expression ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24419
更新日期:2014-01-01 00:00:00
abstract::Ischemia is a situation occurring in several diseases including myocardial infarction and organ transplantation in which oxygenated blood supply is impaired. Ischemia leads to many cellular and tissue modifications, the most important one being cell death. Several explanations have been proposed to account for these m...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041520127
更新日期:1992-07-01 00:00:00
abstract::Nemo-like kinase (Nlk) is related to the mitogen-activated protein (MAP) kinases and known to regulate signaling pathways involved in osteoblastogenesis. In vitro Nlk suppresses osteoblastogenesis, but the consequences of the Nlk inactivation in the skeleton in vivo are unknown. To study the function of Nlk, Nlk(loxP/...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24625
更新日期:2014-11-01 00:00:00
abstract::Cadmium (Cd) is a toxic metal with multiple effects on cell signaling and cell death. We studied the effects of Cd(2+) on quiescent mouse mesangial cells in serum-free conditions. Cadmium induces cell death over 6 h through annexin V+ states without or with causing uptake of propidium iodide, termed apoptotic and apop...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21499
更新日期:2008-11-01 00:00:00
abstract::Cultivation of human parotid glands in serum-free medium (Ca(2+) concentration, 0.2 mM) with growth supplements resulted in isolation of a homogeneous population of epithelial cells without any mesenchymal cells. The isolated cells showed an undifferentiated phenotype with scant cytoplasmic organelles, and low levels ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10149
更新日期:2002-10-01 00:00:00
abstract::Arterial smooth muscle cells undergo marked biochemical and morphological changes upon culturing. We have studied the time course of these changes in smooth muscle cells isolated from normal rabbit aortas by enzymic digestion and then maintained in Dulbecco's modified Eagle's medium with or without 10% rabbit serum. S...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041080321
更新日期:1981-09-01 00:00:00
abstract::Bone morphogenetic proteins (BMPs) play pivotal roles in bone and cartilage growth and repair. Through phenotypes of short-ear (se) mice, which have BMP-5 mutations, a role for BMP-5 in some specific aspects of skeletogenesis and cartilage growth is known. This report examines BMP-5 expression in the growth plate and ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21164
更新日期:2008-01-01 00:00:00
abstract::Excessive generation of mitochondrial reactive oxygen species (ROS) is considered to be initiating event in the development of diabetic nephropathy (DN). Mitochondrial biosynthesis mediated by coactivator PGC-1α and its downstream transcription factors NRF1 and TFAM may be a key target in maintaining mitochondrial fun...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27306
更新日期:2019-04-01 00:00:00
abstract::A sensitive serum-free culture system was used to demonstrate that cells derived from normal human skin release soluble mediators which can modulate keratinocyte, melanocyte, and fibroblast growth in vitro. In M199 supplemented with epidermal growth factor, hydrocortisone, insulin, transferrin, triidothyronine, bovine...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041170215
更新日期:1983-11-01 00:00:00