Abstract:
:A cloned human hepatoma cell line (Li-7A), possessing epidermal growth factor (EGF) receptors numbering in the range of 10-20 pmol/10(6) cells, was inhibited in its growth by EGF as well as an antagonist monoclonal antibody (MoAb) to the EGF receptor. The mode of action of the two ligands of EGF receptors appeared to be different as indicated by the following results: 1) EGF induced marked alteration in cell morphology, whereas the antibody did not; 2) cellular protein accumulated in the EGF-treated cells but not in the antibody treated cells; and 3) ectoATPase activities were greatly enhanced in Li-7A cells treated with EGF and cholera toxin but were unaffected in cells treated with antibody and cholera toxin. The last result also suggests that expression of ectoATPase activities is under the regulation of both EGF and cholera toxin. Li-7A cells provide an additional valuable experimental system for the study of EGF action, as well as the interactive effects of EGF and cholera toxin. The enrichment of the ATPase activities in the EGF-cholera toxin-treated cells can be exploited for the detailed study and isolation of these enzymes and elucidation of their physiological functions.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Knowles AFdoi
10.1002/jcp.1041340113subject
Has Abstractpub_date
1988-01-01 00:00:00pages
109-16issue
1eissn
0021-9541issn
1097-4652journal_volume
134pub_type
杂志文章abstract::Long suspected, recently recognized, and increasingly studied, non protein-coding RNAs (ncRNAs) are emerging as key drivers of biological control and pathology. Since their discovery in 1993, microRNAs (miRNAs) have been the subject of intense research focus and investigations have revealed striking findings, establis...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.24834
更新日期:2015-03-01 00:00:00
abstract::Activation of the receptor tyrosine kinase c-kit by the kit-ligand, also known as stem cell factor (SCF), is essential to melanocyte and germ cell development and during the early stages of hematopoiesis. Deregulated expression of c-kit has been reported in malignancies affecting these lineages, i.e., myeloid leukemia...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199707)172:1<1::AID-JCP1>3
更新日期:1997-07-01 00:00:00
abstract::Serine racemase (SR) is responsible for the biosynthesis of D-serine (D-Ser), an endogenous co-agonist for the glycine (Gly)-binding site on N-methyl-D-aspartate (NMDA) receptors, from L-Ser in the brain. We have previously demonstrated high expression of SR by chondrocytes in cartilage. In this study, we attempted to...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21821
更新日期:2009-09-01 00:00:00
abstract::Obesity predisposes to glucose intolerance and type 2 diabetes (T2D). This disease is often characterized by insulin resistance, changes in insulin clearance, and β-cell dysfunction. However, studies indicate that, for T2D development, disruptions in glucagon physiology also occur. Herein, we investigated the involvem...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27455
更新日期:2019-05-01 00:00:00
abstract::Recently, after the identification of ferritin light chain (L-ferritin) gene and protein over-expression in human metastatic melanoma cells, we engineered, starting from the LM metastatic melanoma cell line, clones in which L-ferritin gene expression was down-regulated by the stable expression of a specific antisense ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20543
更新日期:2006-03-01 00:00:00
abstract::The tumor microenvironment significantly affects tumor progression, and tumor cells can also remodel the tumor microenvironment through complex interaction. Inflammasomes are innate immune system receptors/sensors that regulate an inflammatory response mainly mediated by the nucleotide-binding oligomerization domain-l...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.30169
更新日期:2020-11-23 00:00:00
abstract::An in-depth understanding of circular RNAs (circRNAs) indicates that they are abundant in the eukaryotic transcriptome. Many circRNAs act as microRNA sponges; thus, they represent a new type of regulatory factor. However, the role of circRNA in colorectal cancer (CRC) remains largely unknown. Low circ_0021977 expressi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29135
更新日期:2020-03-01 00:00:00
abstract::The presence of an appropriate number of viable cells is prerequisite for successive differentiation during chondrogenesis. Chondrogenic differentiation has been reported to be influenced by mechanical stimuli. This research aimed to study the effects of cyclic compressive stress on cell viability of rat bone marrow-d...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24359
更新日期:2013-09-01 00:00:00
abstract::Acupuncture needle rotation has been previously shown to cause specific mechanical stimulation of subcutaneous connective tissue. This study uses acupuncture to investigate the role of mechanotransduction-based mechanisms in mechanically-induced cytoskeletal remodeling. The effect of acupuncture needle rotation was qu...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20623
更新日期:2006-06-01 00:00:00
abstract::Hedgehog (Hh) family members are involved in multiple cellular processes including proliferation, migration, differentiation, and cell fate determination. Recently, the novel Hh agonists Hh-Ag 1.3 and 1.7 were identified in a high-throughput screening of small molecule compounds that activate the expression of Gli1, a...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24823
更新日期:2015-04-01 00:00:00
abstract::Acidic fibroblast growth factor (aFGF) mRNA was detected in a rat mammary fibroblastic cell line, but not in rat mammary epithelial cell lines or myoepithelial-like cell lines. Basic FGF (bFGF) mRNA was detected in both the fibroblasts and the myoepithelial-like cells, but was absent from the epithelial cells. A serie...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041440220
更新日期:1990-08-01 00:00:00
abstract::Platelet-derived growth factor (PDGF) is mitogenic and chemotactic for vascular smooth muscle cells cultured in vitro, and, thus, may play a role in the smooth muscle cell proliferation and migration that occurs during atherosclerotic lesion development. Two related PDGF polypeptides, designated as the A and B chains,...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041470116
更新日期:1991-04-01 00:00:00
abstract::Ganoderma lucidum is used in traditional Chinese medicine to prevent or treat a variety of diseases, including cardiovascular disorders. We previously demonstrated that a glucan-containing extract of Reishi polysaccharides (EORP) has the potent anti-inflammatory action of reducing ICAM-1 expression in lipopolysacchari...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.23053
更新日期:2012-08-01 00:00:00
abstract::We recently reported that peptidoglycan (PGN), a cell wall component of the Gram-positive bacterium, induces NF-κB activation and microglia activation. However, PGN-regulated AP-1 activation and cytokine expression in microglia remains unclear. This study investigated how PGN influences the signaling pathway involved ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22489
更新日期:2011-06-01 00:00:00
abstract::Cultured rat embryo cells were stimulated to rapidly release a small group of proteins that included several heat-shock proteins (hsp110, hsp71, hscp73) and nonmuscle actin. The extracellular proteins were analyzed by two-dimensional polyacrylamide gel electrophoresis. Heat-shocked cells released the same set of prote...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041380206
更新日期:1989-02-01 00:00:00
abstract::The presence and origin of mast-cell precursors fixed in the skin tissue of mice were investigated. Giant granules of beige (C57BL/6-bh'/bg', Chediak-Higashi syndrome) mice were used to distinguish different populations of mast cells. Pieces of the skin were grafted from the intact WBB6F1 (WB X C57BL/6)F1-+/+ mice int...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041080315
更新日期:1981-09-01 00:00:00
abstract::The differentiation of mesenchymal stem cells (MSCs) into type II alveolar epithelial (AT II) cells in vivo and in vitro, is critical for reepithelization and recovery in acute lung injury (ALI), but the mechanisms responsible for differentiation are unclear. In the present study, we investigated the role of the canon...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24282
更新日期:2013-06-01 00:00:00
abstract::The main aim of this investigation was to determine whether a functional relationship existed between epidermal growth factor (EGF) and voltage-gated sodium channel (VGSC) upregulation, both associated with strongly metastatic prostate cancer cells. Incubation with EGF for 24 h more than doubled VGSC current density. ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21289
更新日期:2008-04-01 00:00:00
abstract::alpha-Thrombin, bradykinin, and histamine are endogenous mediators that increase endothelial permeability. We examined the mechanism by which these three vasoactive mediators could alter permeability to albumin of human umbilical vein endothelial cells (HUVEC). HUVEC were grown to confluence on Transwell membranes and...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199606)167:3<562::AID-JCP2
更新日期:1996-06-01 00:00:00
abstract::The existence of cancer stem cells is debatable in numerous solid tumors, yet in leukemia, there is compelling evidence of this cell population. Leukemic stem cells (LSCs) are altered cells in which accumulating genetic and/or epigenetic alterations occur, resulting in the transition between the normal, preleukemic, a...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.29394
更新日期:2020-06-01 00:00:00
abstract::Primary astroglial cultures were used to compare the relationships to cell cycling of dolichol-linked glycoprotein synthesis, and of availability of mevalonate, the precursor of dolichol and other isoprenoid lipids. With shift-up to 10% serum (time 0) after 48 h of serum depletion, the proportion of cells in S phase (...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041490215
更新日期:1991-11-01 00:00:00
abstract::Besides the liver, it has been difficult to identify which organ(s) and/or cellular component(s) contribute significantly to the production of human FVIII:c (FVIII). Thus far, only endothelial cells have been shown to constitute a robust extrahepatic source of FVIII, possibly explaining both the diverse presence of FV...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24247
更新日期:2013-05-01 00:00:00
abstract::Ehrlich ascites tumor cells from the plateau phase of growth were transplanted into new hosts, pulse-labeled with tritiated thymidine and blocked with repeated injections of vinblastine. When unlabeled cells were analyzed for their cellular DNA content utilizing a cytophotometric technique it was found that in relatio...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040900218
更新日期:1977-02-01 00:00:00
abstract::Polyploidy has been linked to tumorigenicity mainly due to the chromosomal aberrations. Elevated reactive oxygen species (ROS) generation, on the other hand, has also been associated with oncogenic transformation in most cancer cells. However, a possible link between ploidy and ROS is largely unexplored. Here we have ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22793
更新日期:2012-02-01 00:00:00
abstract::The human polyomavirus, JC virus (JCV), provides an excellent model system to investigate the reciprocal interaction of the immune and nervous systems. Infection with JCV occurs during childhood and the virus remains in the latent state with no apparent clinical symptoms. However, under immunosuppressed conditions, th...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.10096
更新日期:2002-06-01 00:00:00
abstract::This study primarily explored how miR-145, mitogen-activated protein kinase (MAPK) signaling and a downstream transcription factor (i.e., SOX9) mediated development of hypospadias. The hypospadias tissues and preputial tissues were isolated from pediatric inpatients postoperatively. Simultaneously, the rat models of h...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27708
更新日期:2019-07-01 00:00:00
abstract::Papillary thyroid carcinoma (PTC) is a common malignancy in thyroid tissue. However, the molecular mechanism of PTC tumor progression remains unknown. The hedgehog (Hh) pathway is thought to play a key role during PTC development. Here we investigate the effects of glioma-associated oncogene protein-2 (Gli2), an impor...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28399
更新日期:2019-08-01 00:00:00
abstract::Wnt5a, a member of the noncanonical Wnt proteins, is known to play important roles in the development of various organs and in postnatal cell functions. However, little is known about the effects of Wnt5a on human periodontal ligament (PDL) cells. In this study, we examined the localization and potential function of W...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24950
更新日期:2015-11-01 00:00:00
abstract::MicroRNAs play an important regulatory role in the proliferation and differentiation of skeletal muscle-derived satellite cells (MDSCs). In particular, miR-139 can inhibit tumor cell proliferation and invasion, and its expression is down-regulated during C2C12 myoblast differentiation. The aim of this study was thus t...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26817
更新日期:2018-01-01 00:00:00
abstract::To evaluate the effects of fatty acids on endoplasmic reticulum (ER) stress, oxidative stress, and lipid damage. We treated BRL3A rat liver cells with, linoleic (LA), linolenic, oleic (OA), palmitic (PA), palmitoleic (POA), or stearic (SA) acid for 12 hr. The characteristics of cell lipid deposition, oxidative stress ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29553
更新日期:2020-09-01 00:00:00