Abstract：
:This study primarily explored how miR-145, mitogen-activated protein kinase (MAPK) signaling and a downstream transcription factor (i.e., SOX9) mediated development of hypospadias. The hypospadias tissues and preputial tissues were isolated from pediatric inpatients postoperatively. Simultaneously, the rat models of hypospadias were established, and spermatogonial stem cells were separated. The expressions of proteins that symbolized cell apoptosis and oxidative stress were quantified via western blot analysis. Furthermore, the apoptosis, proliferation, and viability of cells were evaluated by means of flow cytometry, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and colony formation assays. The results of microarray indicated miR-145 as a differentially expressed biomarker between hypospadias tissues and normal tissues (p < 0.05). Moreover, rat models of hypospadias were observed with markedly lower vitamins A and E levels, reduced expressions of proteins relevant to oxidative stress (i.e., Nrf2, HO-1, Gpx, and SOD-1), as well as enhanced Bax and cleaved caspase-3 expressions ( p < 0.05). Furthermore, SOX9 was found to be targeted by miR-145, and it was also modified by phosphorylated extracellular-regulated kinase (p-ERK), a portion of MAPK signaling ( p < 0.05). The p-ERK was significantly regulated after altering the expression of miR-145 ( p < 0.05). Moreover, activation of p-ERK and transfection of pcDNA-SOX9 could cause higher expression of apoptins and larger apoptotic proportion of cells ( p < 0.05), yet transfection of miR-145 mimic led to improved cell apoptosis and depressed cell viability ( p < 0.05). In conclusion, SOX9, which was regulated by both miR-145 and miR-145/MAPK signaling, could be involved in the pathogenesis of hypospadias.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Shang Y,Kang Y,Sun J,Wei P,Yang J,Zhang Hdoi
10.1002/jcp.27708subject
Has Abstractpub_date
2019-07-01 00:00:00pages
10397-10410issue
7eissn
0021-9541issn
1097-4652journal_volume
234pub_type
杂志文章abstract：:We investigated the role of mammalian target of rapamycin/nuclear factor-kappa B (mTOR/NF-κB) signaling pathway in high thoracic epidural anesthesia (HTEA) against myocardial ischemia-reperfusion (I/R) injury in rats. The rat model of myocardial I/R injury was established. Ninety rats were divided into the normal, sha...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.26320
更新日期：2018-09-01 00:00:00
abstract：:The mammalian olfactory system is a physiologically plastic region of the brain with the potential to support implanted stem cells. We performed direct injection of lineage-negative (lin-neg), green fluorescent protein-positive (GFP+) bone marrow cells into the olfactory bulb to assess cell survival and motility withi...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20123
更新日期：2005-01-01 00:00:00
abstract：:A mutant of BHK cells (ts422E) temperature-sensitive for processing 32S rRNA to 28S rRNA (Toniolo et al., '73) also loses the ability to synthesize polyamines and 5.8S rRNA when shifted to the non-permissive temperature (39 degrees). The activity of several enzymes not involved with polyamine synthesis, methylation of...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041010303
更新日期：1979-12-01 00:00:00
abstract：:Pathological calcification represents an event that consequently leads to a distinct elevation in the morbidity and mortality of patients with chronic kidney disease (CKD) in addition to strengthening its correlation with hyperphosphatemia. Epigenomic regulation by specific microRNAs (miRNAs) is reported to be involve...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28130
更新日期：2019-08-01 00:00:00
abstract：:Circular RNAs (circRNAs) have spurred considerable interest in numerous tumors. We aimed to investigate the clinical, prognostic, and diagnostic roles of circRNAs in human lung cancer. We systematically searched PubMed, Web of Science, EMBASE, Scopus, CBM, and the Cochrane Library databases up to July 24, 2018. Eligib...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28481
更新日期：2019-08-01 00:00:00
abstract：:The F-40 cell line, a stable variant of LM fibroblasts selected for its resistance to polyethylene glycol (PEG)-induced fusion (Roos and Davidson: Somatic Cell and Molecular Genetics 6:381-391, 1980), has a decreased capacity to internalize fluid-phase markers and nonspecifically surface-bound macromolecules. It is no...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041370314
更新日期：1988-12-01 00:00:00
abstract：:The insulin-like growth factors (IGFs) are potent mitogens for malignant cell proliferation. The majority of secreted IGFs are bound to specific IGF-binding proteins (IGFBPs) that are secreted by a large number of cells. These proteins may either inhibit or enhance IGF actions. Breast carcinoma cells secrete a variety...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041580110
更新日期：1994-01-01 00:00:00
abstract：:The receptor activator of nuclear factor-kappaB ligand (RANKL) plays a critical role in the differentiation and bone resorptive activity of osteoclasts. Recently, the development of anti-resorptive agents from natural substances has become a subject of interest. Therefore, we evaluated the effects of 222 natural compo...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21892
更新日期：2009-12-01 00:00:00
abstract：BACKGROUND:Patients with chronic kidney disease (CKD) have a severe vitamin D deficiency and increasing epidemiological data suggesting that this deficiency may play a role in overall morbidity and mortality associated with CKD. It is known that vitamin D regulates the immune system, however, in dialysis patients this ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28290
更新日期：2019-02-25 00:00:00
abstract：:Although immortalized cultured cells are useful for various functional assays or transcriptome analysis, highly efficient and reproducible immortalization methods have not been developed in avian-derived cells. We introduced the simian virus 40 T antigen (SV40T) and human papillomavirus (HPV)-E6E7 to chick and Okinawa...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27417
更新日期：2019-05-01 00:00:00
abstract：:The retinal pigment epithelium is a monolayer of highly specialized pigmented cells located between the neural retina and the Bruch's membrane of the choroid. RPE cells play a crucial role in the maintenance and function of the underlying photoreceptors. This study introduces a spontaneously arising human retinal pigm...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.25729
更新日期：2017-10-01 00:00:00
abstract：:Neuroblastoma (NBL) exists in a complex tumor-immune microenvironment. Immune cell infiltration and tumor-immune molecules play a critical role in tumor development and significantly impact the prognosis of patients. However, the molecular characteristics describing the NBL-immune interaction and their prognostic pote...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29842
更新日期：2021-01-01 00:00:00
abstract：:Programmed cell death in mammary tissue was studied during natural weaning in lactating mice and after litter removal or milk stasis. All treatments stimulated mammary apoptosis, indicating that this process is an integral part of the tissue's involution after lactation. Induction of apoptosis was slower in natural we...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199609)168:3<559::AID-JCP8
更新日期：1996-09-01 00:00:00
abstract：:Many mammalian SWI/SNF complexes use Brahma-related gene 1 (Brg1) as a catalytic subunit to remodel nucleosomes for transcription regulation. In several mesenchymal cells and tissues, expression of a defective Brg1 protein negates the normal activity of the SWI/SNF complex and delays or blocks differentiation. To inve...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20432
更新日期：2006-01-01 00:00:00
abstract：:Peroxisome proliferator-activated receptor-γ coactivator-1 deficient (Pgc-1β-/- ) murine hearts model the increased, age-dependent, ventricular arrhythmic risks attributed to clinical conditions associated with mitochondrial energetic dysfunction. These were accompanied by compromised action potential (AP) upstroke ra...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27183
更新日期：2019-04-01 00:00:00
abstract：:Effects of interstitial fluid flow on osteoblasts were investigated. Intracellular cyclic adenosine monophosphate (cAMP) levels were monitored in cultured osteoblasts subjected to shear rates ranging from 10 to 3,500 sec-1. Cyclic AMP levels were significantly increased at all shear rates from 1 pmole/mg protein to 10...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041430113
更新日期：1990-04-01 00:00:00
abstract：:Oxidized-low density lipoprotein (Ox-LDL) has been shown to play an important role in impaired surfactant metabolism and transforming growth factor-β1 (TGF-β1) is a critical mediator in the pathogenesis of acute lung injury (ALI)/acute respiratory distress syndrome (ARDS). In this study, we investigated whether Ox-LDL...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.24005
更新日期：2012-09-01 00:00:00
abstract：:Three-dimensional (3D) culture platforms have been explored to establish physiologically relevant cell culture environment and permit expansion scalability; however, little is known about the mechanisms underlying the regulation of pluripotency of human induced pluripotent stem cells (hiPSCs). This study elucidated ep...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.30211
更新日期：2020-12-10 00:00:00
abstract：:Exposure to D-allose has been demonstrated to lead to decreased 2-deoxy-D-glucose (2-DG) and 3-0-methyl-D-glucose transport in the V79 Chinese hamster lung fibroblast cell line. The effect of D-allose 1) was maximal after 4 hours exposure to the cells; 2) was optimal between 2.77 and 5.55 mM D-allose; and 3) led to a ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041450217
更新日期：1990-11-01 00:00:00
abstract：:A process of unconventional secretion that is dependent on the Golgi stacking protein GRASP and multiple components of the autophagy machinery has recently been documented for several cytoplasmic and membrane protein. Classical secretion via the exocytic pathway is inhibited during cell division in animal cells, as ke...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.24264
更新日期：2013-05-01 00:00:00
abstract：:The present work aimed at determining whether interleukin-6 (IL-6) produced by skeletal muscle during exercise is related, at least partly, to calcineurin activity. Rats were treated with two specific calcineurin inhibitors, cyclosporin A (CsA) and FK506, or vehicle (Vhl); they were then subjected to exhaustive treadm...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20854
更新日期：2007-03-01 00:00:00
abstract：:We previously demonstrated that, in the MC615 cartilage cell line, the p38/NF-kB pathway is activated both during differentiation and in response to an inflammatory stimulus. In both cases, the p38/NF-kB pathway activation leads to the expression of the lipocalin SIP24 and of COX-2. Given the fact that, in the same ce...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21516
更新日期：2008-11-01 00:00:00
abstract：:Guanine nucleotides and pertussis toxin were used to test for the involvement of a guanine nucleotide binding protein in the vasopressin V1 receptor-mediated stimulation of protein kinase C activity in Swiss 3T3 cells. Addition of vasopressin in the presence of [gamma-32P]ATP and digitonin caused a marked and rapid in...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041410204
更新日期：1989-11-01 00:00:00
abstract：:Mesenchymal stromal cells (MSCs) have been widely exploited for the treatment of several conditions due to their intrinsic regenerative and immunomodulatory properties. MSC have demonstrated to be particularly relevant for the treatment of ischemic diseases, where MSC-based therapies can stimulate angiogenesis and ind...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29622
更新日期：2020-10-01 00:00:00
abstract：:The relationship of cell surface changes to proliferative decline of human diploid fibroblasts was investigated using the concanavalin A-mediated red blood cell adsorption assay. The amount of the red blood cells adsorbed to human diploid fibroblasts via concanavalin A increased continuously from the early phases of c...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041000219
更新日期：1979-08-01 00:00:00
abstract：:Visualizing protein dynamics is the key to a quantitative understanding of molecular mechanisms in biological systems. Recent developments in fluorescent microscopy techniques allow for novel experimental approaches to study stochastic localization, distribution, and movement of fluorescently labeled molecules in high...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.25451
更新日期：2017-01-01 00:00:00
abstract：:Suppression of PKC activity can selectively induce apoptosis in cells expressing a constitutively activated p21Ras protein. We demonstrate that continued expression of p21Ras activity is required in PKC-mediated apoptosis because farnesyltransferase inhibitors abrogated the loss of viability in p21Ras-transformed cell...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.10409
更新日期：2004-02-01 00:00:00
abstract：:p120-catenin (p120) is an important regulator in the function and stability of E-cadherin. However, the role of p120 in the epidermis is unclear. Previous studies have shown that globally knockout of p120 caused increased epidermal proliferation but little changes in epidermal differentiation and permeability. In the ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.26535
更新日期：2018-01-01 00:00:00
abstract：:As a natural metabolite of limonoids from Dictamnus dasycarpus, fraxinellone has been reported to be neuroprotective and anti-inflammatory. However, its influence on cellular metabolism remains largely unknown. In the present study, we investigated the effect of fraxinellone on cellular senescence-induced by oxidative...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.26169
更新日期：2018-05-01 00:00:00
abstract：:The tumor-promoting phorbol ester, 12-0-tetradecanoyl-phorbol-13-acetate (TPA), stimulates starch-elicited mouse peritoneal macrophages to undergo DNA synthesis in vitro, apparently without the generation of an endogenous macrophage growth factor (MGF). No evidence was found for any synergistic interaction between TPA...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041150111
更新日期：1983-04-01 00:00:00
