The impact of culture dimensionality on behavioral epigenetic memory contributing to pluripotent state of iPS cells.


:Three-dimensional (3D) culture platforms have been explored to establish physiologically relevant cell culture environment and permit expansion scalability; however, little is known about the mechanisms underlying the regulation of pluripotency of human induced pluripotent stem cells (hiPSCs). This study elucidated epigenetic modifications contributing to pluripotency of hiPSCs in response to 3D culture. Unlike two-dimensional (2D) monolayer cultures, 3D cultured cells aggregated with each other to form ball-like aggregates. 2D cultured cells expressed elevated levels of Rac1 and RhoA; however, Rac1 level was significantly lower while RhoA level was persisted in 3D aggregates. Compared with 2D monolayers, the 3D aggregates also exhibited significantly lower myosin phosphorylation. Histone methylation analysis revealed remarkable H3K4me3 upregulation and H3K27me3 maintenance throughout the duration of 3D culture; in addition, we observed the existence of naïve pluripotency signatures in cells grown in 3D culture. These results demonstrated that hiPSCs adapted to 3D culture through alteration of the Rho-Rho kinase-phospho-myosin pathway, influencing the epigenetic modifications and transcriptional expression of pluripotency-associated factors. These results may help design culture environments for stable and high-quality hiPSCs.


J Cell Physiol


Thanuthanakhun N,Kino-Oka M,Borwornpinyo S,Ito Y,Kim MH




Has Abstract


2020-12-10 00:00:00






  • Characterization of a simian virus 40-transformed human podocyte cell line producing type IV collagen and exhibiting polarized response to atrial natriuretic peptide.

    abstract::Biology of glomerular visceral epithelial cells ("podocytes") and their role in inflammatory process remain obscure, partly because of the lack of well-differentiated podocyte cultures. We have established a human cell line by transfecting with a replication-defective SV40 plasmid (pSVHB1), a primary culture of podocy...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ardaillou N,Lelongt B,Turner N,Piedagnel R,Baudouin B,Estrade S,Cassingena R,Ronco PM

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    abstract::The biosynthesis and assembly of the peripheral sector (V1) of the vacuolar proton-translocating adenosine triphosphatase (V-ATPase) was studied in a bovine kidney epithelial cell line. Monolayer cultures of cells were metabolically radiolabeled with Tran35S-label and the V-ATPase subsequently immunoprecipitated using...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


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    abstract::Release of transcellular tension upon disruption of actin stress fibers with cytochalasin D (CD) and associated changes in cell morphology are reflected in the rapid transcription of "deformation-responsive" genes. For certain genes (e.g., urokinase plasminogen activator and its type-1 inhibitor PAI-1), de novo mRNA s...

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  • Sulfated malto-oligosaccharides bind to basic FGF, inhibit endothelial cell proliferation, and disrupt endothelial cell tube formation.

    abstract::The interaction of basic FGF (bFGF) with heparin, heparan sulfate and related sugars can potentiate or antagonize bFGF activity, depending on the size of the saccharide used. Oligosaccharides based on heparin structures, as small as six sugar residues, have been demonstrated to bind to bFGF and block its activity, whi...

    journal_title:Journal of cellular physiology

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  • Survivin modulatory role in autoimmune and autoinflammatory diseases.

    abstract::Baculoviral IAP repeat containing 5 (BIRC5) gene encodes the important protein as survivin, a multifunctional protein, which is involved in cellular and molecular networks, progression of cell cycle, homeostasis, developmental morphogenesis, and apoptosis. The proximal BIRC5 promoter possesses specific binding sites f...

    journal_title:Journal of cellular physiology

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  • Toll-like receptors in the functional orientation of cardiac progenitor cells.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


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  • miR-142a-5p promoted osteoblast differentiation via targeting nuclear factor IA.

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  • Hypoxia induces epidermal keratinocyte matrix metalloproteinase-9 secretion via the protein kinase C pathway.

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    pub_type: 杂志文章


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  • Lysophosphatidic acid induces the crosstalk between the endovascular human trophoblast and endothelial cells in vitro.

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    pub_type: 杂志文章,评审


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    pub_type: 杂志文章


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    pub_type: 杂志文章,评审


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    pub_type: 杂志文章


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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


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  • Effect of donor age and prior sun exposure on growth inhibition of cultured human dermal fibroblasts by all trans-retinoic acid.

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Morrione A

    更新日期:2003-12-01 00:00:00

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Katayama M,Kiyono T,Ohmaki H,Eitsuka T,Endoh D,Inoue-Murayama M,Nakajima N,Onuma M,Fukuda T

    更新日期:2019-05-01 00:00:00

  • Quercetin protects against radiocontrast medium toxicity in human renal proximal tubular cells.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


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    更新日期:2018-05-01 00:00:00

  • Calcium sources used by post-natal human myoblasts during initial differentiation.

    abstract::Increases in cytoplasmic Ca(2+) are crucial for inducing the initial steps of myoblast differentiation that ultimately lead to fusion; yet the mechanisms that produce this elevated Ca(2+) have not been fully resolved. For example, it is still unclear whether the increase comes exclusively from membrane Ca(2+) influx o...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Arnaudeau S,Holzer N,König S,Bader CR,Bernheim L

    更新日期:2006-08-01 00:00:00

  • Dauricine induces apoptosis, inhibits proliferation and invasion through inhibiting NF-kappaB signaling pathway in colon cancer cells.

    abstract::Dauricine, a bioactive component of Asiatic Moonseed Rhizome, has been widely used to treat a large number of inflammatory diseases in traditional Chinese medicine. In our study, we demonstrated that dauricine inhibited colon cancer cell proliferation and invasion, and induced apoptosis by suppressing nuclear factor-k...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Yang Z,Li C,Wang X,Zhai C,Yi Z,Wang L,Liu B,Du B,Wu H,Guo X,Liu M,Li D,Luo J

    更新日期:2010-10-01 00:00:00

  • Characterizing the multiple roles of FGF-2 in SOD1G93A ALS mice in vivo and in vitro.

    abstract::We have previously shown that knockout of fibroblast growth factor-2 (FGF-2) and potential compensatory effects of other growth factors result in amelioration of disease symptoms in a transgenic mouse model of amyotrophic lateral sclerosis (ALS). ALS is a rapidly progressive neurological disorder leading to degenerati...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Kefalakes E,Böselt S,Sarikidi A,Ettcheto M,Bursch F,Naujock M,Stanslowsky N,Schmuck M,Barenys M,Wegner F,Grothe C,Petri S

    更新日期:2019-05-01 00:00:00

  • Brg1, the ATPase subunit of the SWI/SNF chromatin remodeling complex, is required for myeloid differentiation to granulocytes.

    abstract::Many mammalian SWI/SNF complexes use Brahma-related gene 1 (Brg1) as a catalytic subunit to remodel nucleosomes for transcription regulation. In several mesenchymal cells and tissues, expression of a defective Brg1 protein negates the normal activity of the SWI/SNF complex and delays or blocks differentiation. To inve...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


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    更新日期:2006-01-01 00:00:00