Abstract：
:The objective of this study was to establish whether human recombinant tumor necrosis factor (TNF) can significantly stimulate the proliferation of some tumor cells. Treatment with TNF had little or no effect on the growth of human tumor cells and murine NIH/3T3 cells cultured in medium with high serum concentration. Two tumor lines, SK-MEL-109 melanoma and HOS osteosarcoma cells, were adapted to grow in medium supplemented with 0.5% serum. The growth of these SK-MEL-109 cells was inhibited by TNF, but that of the HOS cells was greatly stimulated by TNF in a dose-dependent way. Treatment with 10 ng/ml of TNF resulted in a two-fold increase in the rate of cell division. This effect of TNF was also shown by measuring DNA and protein synthesis. The continuous presence of TNF was not required for its mitogenic activity on HOS cells cultured with 0.5% serum, since treatment for only one day with TNF resulted in prolonged growth stimulation. The failure of TNF to promote division of cells cultured in medium with 10% serum may possibly be explained by the presence of saturating amounts of growth factors in serum. Interferons abolished the mitogenic activity of TNF on HOS cells. Furthermore, TNF did not show synergism with insulin or epidermal growth factor in stimulating growth of these cells. The level of c-myc mRNA was increased five-fold after 30 minutes of treatment with TNF. This shows that TNF is a growth factor for HOS cells and that it induces accumulation of c-myc mRNA.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Kirstein M,Baglioni Cdoi
10.1002/jcp.1041340321subject
Has Abstractpub_date
1988-03-01 00:00:00pages
479-84issue
3eissn
0021-9541issn
1097-4652journal_volume
134pub_type
杂志文章abstract：:We demonstrated previously that in serum-starved MCF-7 breast cancer cell line, Ang II increased Na+/K+ATPase activity and activated the protein kinase C zeta (PKC-zeta) (Muscella et al., 2002 J Endocrinol 173:315-323; 2003 J Cell Physiol 197:61-68.). The aim of the present study was to investigate the modulation of t...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20396
更新日期：2005-11-01 00:00:00
abstract：:The plasma membrane of erythrocytes, as of other cells, is thought to act as the barrier responsible for maintaining intracellular gradients of most ions and small molecular species between the cell and its environment. Controlled application of the nonionic detergent Brij 58 effectively opened the erythrocyte plasma ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041590202
更新日期：1994-05-01 00:00:00
abstract：:Syndecan-1 belongs to a family of transmembrane proteoglycans, acts as a coreceptor for growth factor binding, as well as cell-matrix and cell-cell interactions, and is induced in smooth muscle cells (SMCs) following balloon catheter injury. In this report, we investigated syndecan-1 expression in SMCs in response to ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20927
更新日期：2007-04-01 00:00:00
abstract：:Primary porcine choroid plexus epithelial cells cultivated in chemically defined medium maintain their epithelial characteristics and form confluent monolayers. They produce a fluid the composition of which resembles cerebrospinal fluid. The present study demonstrates constitutive secretion of large amounts of beta-tr...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199611)169:2<235::AID-JCP2
更新日期：1996-11-01 00:00:00
abstract：:The activation/adaptive responses of human monocytes exposed to Bordetella pertussis parental or mutant strains were evaluated and correlated to the expression of two bacterial toxins: adenylate cyclase-hemolysin and pertussis toxin. The marked rise in intracellular cyclic adenosine monophosphate (cAMP) observed in mo...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(200004)183:1<91::AID-JCP11
更新日期：2000-04-01 00:00:00
abstract：:We have examined nucleoside transport (NT) in a cell line derived from primary day 7 murine bone marrow macrophages (S1 macrophages) in response to the macrophage growth factor, colony-stimulating factor 1 (CSF-1). Adenosine and uridine transport in quiescent S1 macrophages occurred primarily by two facilitated diffus...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041550311
更新日期：1993-06-01 00:00:00
abstract：:Increasing evidence has demonstrated that abnormal expression of lncRNA is correlated with various malignant tumors, including hepatocellular carcinoma (HCC). Our current study was aimed to investigate the role of LINC00707 in HCC development. We observed that LINC00707 was upregulated in HCC cell lines compared with ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27449
更新日期：2019-05-01 00:00:00
abstract：:The platelet-derived growth factor (PDGF) binds via a defined amino acid sequence to heparin (Fager et al., 1992, In Vitro Cell. Dev. Biol., 28A:176-180) and the protein moiety of low density lipoproteins (LDL; apo B-100) via a similar sequence to chondroitin sulfate (Camejo et al., 1988, Arteriosclerosis Thromb., 8:3...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041630218
更新日期：1995-05-01 00:00:00
abstract：:Alcoholic liver disease (ALD) is characterized by hepatocyte damage, inflammatory cell activation, and increased intestinal permeability leading to the clinical manifestations of alcoholic hepatitis. Selected members of the family of microRNAs (miRNAs) are affected by alcohol, resulting in an abnormal miRNA profile in...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.26938
更新日期：2018-01-01 00:00:00
abstract：OBJECTIVE:Disc degeneration is the common life-threatening disease characterized by flank pain. The gene expression of insulin-like growth factor binding protein 3 (IGFBP3) is increased in patients with disc degeneration, however, its mechanism is still unknown. This study aimed to investigate the influence of IGFBP3 g...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27613
更新日期：2019-06-01 00:00:00
abstract：:Tumor ischemia participates in angiogenesis and cancer progression through cellular responses to hypoxia and nutrient deprivation. However, the contribution of amino acids limitation to this process remains poorly understood. Using serum-free cell culture conditions, we tested the impact of L-glutamine deprivation on ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21044
更新日期：2007-08-01 00:00:00
abstract：:Chick embryo fibroblasts were maintained at confluency for up to 35 days in medium containing 0.5% or 0.75% fetal bovine serum or 2.5% or 5.0% horse serum. At weekly intervals cells were subcultured and serially propagated in medium containing 10% FBS until their replicative lifespans were completed. The results showe...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041070308
更新日期：1981-06-01 00:00:00
abstract：:Osteoporosis is a class of metabolic bone disease caused by complexed ramifications. Overactivation of osteoclasts due to a sudden decreased estrogen level plays a pivotal role for postmenopausal women suffering from osteoporosis. Therefore, inhibiting osteoclast formation and function has become a major direction for...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28289
更新日期：2019-02-27 00:00:00
abstract：:Elevated levels of follistatin-like protein 1 (FSTL1) have been found both in mouse models for human rheumatoid arthritis (RA) and collagen-induced arthritis (CIA). In this study, we elucidated the potential mechanisms by which FSTL1 contributes to the pathogenesis of RA. Fibroblast-like synoviocytes (FLSs) were estab...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.26580
更新日期：2018-01-01 00:00:00
abstract：:Effects of interstitial fluid flow on osteoblasts were investigated. Intracellular cyclic adenosine monophosphate (cAMP) levels were monitored in cultured osteoblasts subjected to shear rates ranging from 10 to 3,500 sec-1. Cyclic AMP levels were significantly increased at all shear rates from 1 pmole/mg protein to 10...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041430113
更新日期：1990-04-01 00:00:00
abstract：:Phospholipase C-ε (PLCε) is frequently overexpressed in tumors and plays an important role in the regulation of tumorigenesis. Although great progress has been made in understanding biological roles of PLCε, the relevant molecular mechanisms underlying its pro-tumor activity remain largely unclear. Here, we demonstrat...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29698
更新日期：2020-11-01 00:00:00
abstract：:Melatonin, the major pineal secretory product, has a significant impact on the female reproductive system. Recently, the beneficial effects of melatonin on mammalian oocyte maturation and embryonic development have drawn increased attention. However, the exact underlying mechanisms remain to be fully elucidated. This ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.25876
更新日期：2018-01-01 00:00:00
abstract：:The basis of the well-known decline in cell proliferation with increasing passage number of human diploid fibroblast-like cell cultures is not known. It has been found that DNA synthesis was deficient in the remaining but still proliferating cells, but when appropriate corrections reflecting the remaining dividing cel...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041430107
更新日期：1990-04-01 00:00:00
abstract：:The extracellular matrix (ECM) is present in a range of molecular conformations and intermolecular arrangements. Fibronectin (Fn) molecules that constitute fibers within the ECM can exist in a variety of conformations that result from both mechanical stress and chemical factors such as allosteric binding partners. The...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.25271
更新日期：2016-08-01 00:00:00
abstract：:Syndecans are constitutively shed from growing epithelial cells as the part of normal cell surface turnover. However, increased serum levels of the soluble syndecan ectodomain have been reported to occur during bacterial infections. The aim of this study was to evaluate the potential of lipopolysaccharide (LPS) from t...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20287
更新日期：2005-07-01 00:00:00
abstract：:We have compared the biological and biochemical properties of recombinant PDGF AA, AB, and BB using three types of fibroblastic cells: NIH/3T3, human skin fibroblast, and fetal bovine aortic smooth muscle. PDGF binding, receptor autophosphorylation, phosphatidyl inositol hydrolysis, as well as chemotactic and mitogeni...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041490209
更新日期：1991-11-01 00:00:00
abstract：:Members of the epidermal growth-factor-receptor tyrosine-kinase (EGFR) family play important roles both in normal growth regulation/cell differentiation and in the genesis and progression of human neoplasia. In the present study, we analysed distinct heregulin (HRG) signals mediated by the HRG receptors HER3 and HER4....
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199711)173:2<187::AID-JCP1
更新日期：1997-11-01 00:00:00
abstract：:Despite remarkable progress in polychemotherapy protocols, pediatric B-cell acute lymphoblastic leukemia (B-ALL) remains fatal in around 20% of cases. Hence, novel targeted therapies are needed for patients with poor prognosis. Glucocorticoids (GCs) are drugs commonly administrated for B-ALL treatment. Activation of t...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.26135
更新日期：2018-03-01 00:00:00
abstract：:Human population is facing a revolutionary change in the demographic structure with an increasing number of elderly people requiring an unmet need to ensure a smooth aging process and dental care is certainly an important aspect that has to be considered. To date, dentistry has been conservative and the need of transf...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.25452
更新日期：2017-03-01 00:00:00
abstract：:The initial step in a synthesis of thyroid hormones is the uptake of iodide from the circulation. Iodide (I(-)) is transported into thyroid cells via a Na(+)/I(-) symporter (NIS), which is electrogenic and thus sensitive to alterations in membrane potential (V(m)). I(-) is then released to the lumen of thyroid follicl...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.24230
更新日期：2013-04-01 00:00:00
abstract：:Selenium has been associated with cancer prevention. Despite vast knowledge of selenium effect on various health conditions, functional characterization of selenium metabolic enzymes on cellular physiology has been limited. Therefore, to gain insight into the mechanisms underlying cancer prevention by selenium, we inv...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20714
更新日期：2006-10-01 00:00:00
abstract：:Pulmonary fibrosis is a potentially fatal consequence of treatments for malignancy and is an increasing problem in bone marrow transplant patients and in cases of allogenic lung transplant. The fibrotic response is characterized by increases in lung fibroblast number and collagen synthesis. This laboratory previously ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199605)167:2<290::AID-JCP1
更新日期：1996-05-01 00:00:00
abstract：:The glucose analogues, 3-O-methyl-D-glucose and 2-deoxy-D-glucose, have been used to characterize glucose transport and its regulation by serum and growth factors in monolayer cultures of granulosa cells obtained from bovine ovaries. Uptake of 3-O-methylglucose was shown to be independent of the Na+-gradient, independ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041080104
更新日期：1981-07-01 00:00:00
abstract：:The activity of acidic and basic fibroblast growth factor-like mitogens (aFGF, bFGF) extracted from cultured bovine aortic endothelial (BAEC) and rat aortic smooth muscle cells (SMC) was compared with that of freshly isolated cells from the same tissues. Extracts of subendothelial extracellular matrix (ECM) and cell l...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041470223
更新日期：1991-05-01 00:00:00
abstract：:The progression of cancer is often associated with genomic instability, which may develop as a result of compromised defense mechanisms responsible for the maintenance of chromosomal integrity. These include defects in telomere preservation, chromosomal segregation, and DNA repair. In this review, we discuss molecular...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.20455
更新日期：2006-02-01 00:00:00
