Abstract:
:The purpose of this study was to investigate the potential mechanism of interleukin-6 (IL-6) on the stimulation of excessive androgen secretion in human NCI-H295R adrenocortical cells. We performed transcriptome sequencing of cancer and paracancerous tissues obtained from functional adrenal cortical adenomas. The secretion of dehydroepiandrosterone sulfate (DHEAS) in NCI-H295R cells was detected by a chemiluminescence assay. The expression of messenger RNA (mRNA) was detected by real-time polymerase chain reaction and that of protein was detected by western blotting. The expression of secretogranin II (SCG2) and IL-6 were significantly increased in cancer tissues. Upregulation of mRNA and protein levels of AKR1C3, CYP11A, CYP17A1, 3βHSD, and SULT2A1 was observed after stimulation with IL-6. IL-6 could also increase the expression of StAR mRNA and proteins. Our results suggest that IL-6 can promote androgen secretion by regulating the expression of genes related to androgen pathways.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Wei X,Zhang J,Peng W,Xu H,Wei Z,Pang L,Liu J,Wang Tdoi
10.1002/jcp.29748subject
Has Abstractpub_date
2020-12-01 00:00:00pages
9432-9444issue
12eissn
0021-9541issn
1097-4652journal_volume
235pub_type
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