Phenotypic changes of adult porcine mesenchymal stem cells induced by prolonged passaging in culture.

abstract：:In response to metabolic stress, GLUT4, the most abundant glucose transporter, translocates from intracellular vesicles to the plasma membrane. This appears to play an important role in protecting cardiac myocytes from ischemic injury. To investigate the precise mechanisms of GLUT4 translocation in cardiomyocytes, we ...

journal_title：Journal of cellular physiology

authors： Horie T,Ono K,Nagao K,Nishi H,Kinoshita M,Kawamura T,Wada H,Shimatsu A,Kita T,Hasegawa K

更新日期：2008-06-01 00:00:00

abstract：:Multiple modalities for lung cancer therapy have emerged in the past decade, whereas their clinical applications and survival-beneficiary is little known. Vaccination with dendritic cells (DCs) or DCs/cytokine-induced killer (CIK) cells has shown limited success in the treatment of patients with advanced non-small-cel...

journal_title：Journal of cellular physiology

authors： Mohsenzadegan M,Peng RW,Roudi R

更新日期：2020-01-01 00:00:00

abstract：:In order to study the generation, factors controlling endogenous folate pools, and their functional importance, Friend erythroleukemia cells were grown in media containing 100; 1,000; and 10,000 ng/ml of tritiated pteroylglutamic acid (3H)PteGlu1 and then studied in unlabeled media with varying amounts of PteGlu1. The...

journal_title：Journal of cellular physiology

authors： Steinberg SE,Fonda S,Campbell CL,Hillman RS

更新日期：1983-02-01 00:00:00

abstract：:Differentiation commitment events are essential for the initiation of hemopoiesis and, in one form or another, occur continuously during adult hemopoiesis. The most studied type of differentiation commitment decision a hemopoietic cell can make involves the alternative choice of self-renewal versus the formation of pr...

journal_title：Journal of cellular physiology

authors： Metcalf D,Nicola NA,Robb L

更新日期：1997-11-01 00:00:00

abstract：:To evaluate the regulation of endothelial cell Cu,Zn-SOD, we have exposed bovine pulmonary artery endothelial cells in culture to hyperoxia and hypoxia, second messengers or related agonists, hormones, free radical generating systems, endotoxin, and cytokines and have measured Cu,Zn-SOD protein of these cells by an EL...

journal_title：Journal of cellular physiology

authors： Kong XJ,Fanburg BL

更新日期：1992-12-01 00:00:00

abstract：:Two transport systems for neutral amino acids have been characterised in LLC-PK, cells. The first, which transports alanine in a sodium-dependent manner, also mediates alanine exchange and is perferentially inhibited by serine, cysteine, and alpha-amino-n-butyric acid. This system resembles the ASC system in Ehrlich a...

journal_title：Journal of cellular physiology

authors： Sepúlveda FV,Pearson JD

更新日期：1982-08-01 00:00:00

abstract：:Vitamin E, a dietary antioxidant, is presumed to be incorporated into the lipid bilayer of biological membranes to an extent proportional to the amount of polyunsaturated fatty acids or phospholipids in the membrane. In the present study we evaluated the distribution of incorporated polyunsaturated fatty acids (PUFA) ...

journal_title：Journal of cellular physiology

authors： Sekharam KM,Patel JM,Block ER

更新日期：1990-12-01 00:00:00

abstract：:Glioblastoma multiforme (GBM) cells are characterised by their extreme chemoresistance. The activity of multiple-drug resistance (MDR) transporters that extrude antitumor drugs from cells plays the most important role in this phenomenon. To date, the mechanism controlling the expression and activity of MDR transporter...

journal_title：Journal of cellular physiology

authors： Quezada C,Garrido W,Oyarzún C,Fernández K,Segura R,Melo R,Casanello P,Sobrevia L,San Martín R

更新日期：2013-03-01 00:00:00

abstract：:Confluent cultures of adult bovine aortic endothelial (ABAE), corneal endothelial (BCE), and fetal bovine heart endothelial (FBHE) cells form a monolayer of highly flattened, closely apposed, and nonoverlapping cells. In ABAE and BCE cultures, this is associated with a 50-fold decrease in the rate of DNA sythesis and ...

journal_title：Journal of cellular physiology

authors： Savion N,Vlodavsky I,Greenburg G,Gospodarowicz D

更新日期：1982-02-01 00:00:00

abstract：:A variant of the hypoxanthine-guanine phosphoribosyltransferase deficient, and adenine phosphoribosyltransferase deficient mouse resistant to 6-azauridine. These cells are not only resistant to 6-azauridine (5 X 10(-4) M), but also to adenosine (10(-3) M). Resistance persists indefinitely even in the absence of both c...

journal_title：Journal of cellular physiology

authors： Hashmi S,May SR,Krooth RS,Miller OJ

更新日期：1975-10-01 00:00:00

abstract：:We analyzed the effect of exposure to hypoxic/hypercapnic (HH) gas mixture (5% O2 /9% CO2 ) on the maintenance of functional cord blood CD34(+) hematopoietic stem and progenitor cells in severe hypothermia (4°C) employing the physiological and proteomic approaches. Ten-day exposure to HH maintained the Day 0 (D-0) lev...

journal_title：Journal of cellular physiology

authors： Vlaski M,Negroni L,Kovacevic-Filipovic M,Guibert C,Brunet de la Grange P,Rossignol R,Chevaleyre J,Duchez P,Lafarge X,Praloran V,Schmitter JM,Ivanovic Z

更新日期：2014-12-01 00:00:00

abstract：:Upregulation and overexpression of discoidin domain receptor 1 (DDR1) have been implied in the regulation of kidney development and progression of cancers. Our previous studies with Mardin-Darby canine kidney (MDCK) cells showed that overexpression of DDR1 inhibited cell spreading, whereas dominant negative DDR1 promo...

journal_title：Journal of cellular physiology

authors： Yeh YC,Wang CZ,Tang MJ

更新日期：2009-01-01 00:00:00

abstract：:Binding of concanavalin A to isolated thymocyte membrane vesicles occurs through (a) numerous (approximately 6 x 106/cell equivalent) low-affinity sites (Ka = 1.3 x 105M-1) anf (b) fewer (approximately 0.4 x 106/cell equipment) specific receptors (Ka = 6.8 x 106 M-1) defined as 55,000 D glycoprotein and its multimers....

journal_title：Journal of cellular physiology

authors： Wallach DF,Schmidt-Ullrich R

更新日期：1976-12-01 00:00:00

abstract：:Adsorption of Sendai virus at high multiplicity (500-1,000 HAU/10(6) cells) to HeLa cells grown in monolayers causes immediate changes in the ion barrier of the cell membrane, as well as changes in the morphology of the virus-treated cells. Within minutes of adsorption the cells begin to lose potassium and an extensiv...

journal_title：Journal of cellular physiology

authors： Spiegelstein PF,Haimsohn M,Gitelman J,Kohn A

更新日期：1978-05-01 00:00:00

abstract：:The effects of exposure of cultured P388D1 cells to H2O2 on intracellular free calcium ([Ca++]i) was investigated utilizing the intracellular fluorescent calcium chelator "Quin 2." [Ca++]i rose from approximately 150 nM to greater than 2 microM over a time course that was strongly dependent on the concentration of H2O...

journal_title：Journal of cellular physiology

authors： Hyslop PA,Hinshaw DB,Schraufstätter IU,Sklar LA,Spragg RG,Cochrane CG

更新日期：1986-12-01 00:00:00

abstract：:Dental follicle cells (DFCs) activate and recruit osteoclasts for tooth development and tooth eruption, whereas DFCs themselves differentiate into osteoblasts to form alveolar bone surrounding tooth roots through the interaction with Hertwig's epithelial root sheath (HERS). Also during tooth development, parathyroid h...

journal_title：Journal of cellular physiology

authors： Zhang J,Liao L,Li Y,Xu Y,Guo W,Tian W,Zou S

更新日期：2019-07-01 00:00:00

abstract：:A novel, serum albumin-binding glycoprotein of molecular weight (mw) 43,000 (43K protein) was initially purified from the culture medium of bovine aortic endothelial (BAE) cells (Sage, H., Johnson, C., and Bornstein, P., J. Biol. Chem. 259:3993-4007, 1984). Its secretion by normal mesenchymal cells and by transformed ...

journal_title：Journal of cellular physiology

authors： Sage H,Tupper J,Bramson R

更新日期：1986-06-01 00:00:00

abstract：:TRPV4 (transient receptor potential vanilloid 4) is a cation channel activated by hypotonicity, moderate heat, or shear stress. We describe the expression of TRPV4 during the differentiation of a corneal epithelial cell model, RCE1(5T5) cells. TRPV4 is a late differentiation feature that is concentrated in the apical ...

journal_title：Journal of cellular physiology

authors： Martínez-Rendón J,Sánchez-Guzmán E,Rueda A,González J,Gulias-Cañizo R,Aquino-Jarquín G,Castro-Muñozledo F,García-Villegas R

更新日期：2017-07-01 00:00:00

abstract：:Adult stem cell therapy is being used extensively to rejuvenate damaged tissue. One important tissue source to obtain these cells is adipose, which contains cells called adipose-derived stem cells (ADSCs). These cells have a great therapeutic potential not only for their multipotent properties as well as for immunomod...

journal_title：Journal of cellular physiology

authors： Muñoz MF,Argüelles S,Medina R,Cano M,Ayala A

更新日期：2019-08-01 00:00:00

abstract：:K+ channels of the alveolar epithelium control the driving force acting on the ionic and solvent flow through the cell membrane contributing to the maintenance of cell volume and the constitution of epithelial lining fluid. In the present work, we analyze the effect of the Cl- channel inhibitors: (4-[(2-butyl-6,7-dich...

journal_title：Journal of cellular physiology

authors： Canella R,Martini M,Cavicchio C,Cervellati F,Benedusi M,Valacchi G

更新日期：2019-08-01 00:00:00

abstract：:The purpose of this study was to delineate the traffic patterns of EGF and EGF receptors (EGFR) in primary cultured acinar epithelial cells from rabbit lacrimal glands. Uptake of [(125)I]-EGF exhibited saturable and non-saturable, temperature-dependent components, suggesting both receptor-mediated and fluid phase endo...

journal_title：Journal of cellular physiology

authors： Xie J,Qian L,Wang Y,Rose CM,Yang T,Nakamura T,Hamm-Alvarez SF,Mircheff AK

更新日期：2004-04-01 00:00:00

abstract：:We report the continuous growth maintenance of untransformed and chemically transformed fibroblasts (AKR-2B, AKR-MCA cells) in low concentrations of serum (0.1% FBS). The cell lines established (AKR-0.1F, MCA-0.1F) proliferated at rates comparable to cells maintained under high serum conditions (10% FBS). Complete rem...

journal_title：Journal of cellular physiology

authors： Mulder KM,Brattain MG

更新日期：1989-03-01 00:00:00

abstract：:Exposure of neutrophils to a variety of agonists including soluble chemoattractant peptides and cytokines results in degranulation and activation of the oxidative burst (effector functions) that are required for bacterial killing. At present, the signaling pathways regulating these important functions are incompletely...

journal_title：Journal of cellular physiology

authors： Sue-A-Quan AK,Fialkow L,Vlahos CJ,Schelm JA,Grinstein S,Butler J,Downey GP

更新日期：1997-07-01 00:00:00

abstract：:An efficient regulation of fuel metabolism in response to internal and environmental stimuli is a vital task that requires an intact carnitine system. The carnitine system, comprehensive of carnitine, its derivatives, and proteins involved in its transformation and transport, is indispensable for glucose and lipid met...

journal_title：Journal of cellular physiology

authors： Peluso G,Nicolai R,Reda E,Benatti P,Barbarisi A,Calvani M

更新日期：2000-03-01 00:00:00

abstract：:A number of different organs and tissues from untreated or endotoxin-injected mice were surveyed for their ability to produce conditioned medium that could induce differentiation of the myelomonocytic leukemic cell line WEHI-3B. All organs were able to do this but were much more effective from endotoxin-injected anima...

journal_title：Journal of cellular physiology

authors： Nicola NA,Metcalf D

更新日期：1981-11-01 00:00:00

abstract：:Papillary thyroid carcinoma (PTC) is a common malignancy in thyroid tissue. However, the molecular mechanism of PTC tumor progression remains unknown. The hedgehog (Hh) pathway is thought to play a key role during PTC development. Here we investigate the effects of glioma-associated oncogene protein-2 (Gli2), an impor...

journal_title：Journal of cellular physiology

authors： Ma J,Huang X,Li Z,Shen Y,Lai J,Su Q,Zhao J,Xu J

更新日期：2019-08-01 00:00:00

abstract：BACKGROUND:Surgical stress has been suggested to facilitate colon cancer growth and metastasis. However, the precise mechanisms by which surgical trauma promotes colon cancer progression remain poorly understood. METHODS:To unravel the mechanisms underlying surgery-induced colon cancer progression, a syngenic transpla...

journal_title：Journal of cellular physiology

authors： Sun Z,Du C,Xu P,Miao C

更新日期：2019-04-01 00:00:00

abstract：:Polyploidy has been linked to tumorigenicity mainly due to the chromosomal aberrations. Elevated reactive oxygen species (ROS) generation, on the other hand, has also been associated with oncogenic transformation in most cancer cells. However, a possible link between ploidy and ROS is largely unexplored. Here we have ...

journal_title：Journal of cellular physiology

authors： Roh M,van der Meer R,Abdulkadir SA

更新日期：2012-02-01 00:00:00

abstract：:The human polyomavirus, JC virus (JCV), provides an excellent model system to investigate the reciprocal interaction of the immune and nervous systems. Infection with JCV occurs during childhood and the virus remains in the latent state with no apparent clinical symptoms. However, under immunosuppressed conditions, th...

journal_title：Journal of cellular physiology

authors： Sweet TM,Del Valle L,Khalili K

更新日期：2002-06-01 00:00:00

abstract：:Repetitive mechanical stimulation of cultured avian skeletal muscle increases the synthesis of prostaglandins (PG) E2 and F2 alpha which regulate protein turnover rates and muscle cell growth. These stretch-induced PG increases are reduced in low extracellular calcium medium and by specific phospholipase inhibitors. M...

journal_title：Journal of cellular physiology

authors： Vandenburgh HH,Shansky J,Karlisch P,Solerssi RL

更新日期：1993-04-01 00:00:00