当前位置： SCI文献检索 > JOURNAL OF CELLULAR PHYSIOLOGY期刊下所有文献 > Hypoxia/hypercapnia-induced adaptation maintains functional capacity of cord blood stem and progenitor cells at 4°C.

Hypoxia/hypercapnia-induced adaptation maintains functional capacity of cord blood stem and progenitor cells at 4°C.

Abstract：

:We analyzed the effect of exposure to hypoxic/hypercapnic (HH) gas mixture (5% O2 /9% CO2 ) on the maintenance of functional cord blood CD34(+) hematopoietic stem and progenitor cells in severe hypothermia (4°C) employing the physiological and proteomic approaches. Ten-day exposure to HH maintained the Day 0 (D-0) level of hematopoietic stem cells as detected in vivo on the basis of hematopoietic repopulation of immunodeficient mice-short-term scid repopulating cells (SRC). Conversely, in the atmospheric air (20% O2 /0.05% CO2 ), usual condition used for cell storage at 4°C, stem cell activity was significantly decreased. Also, HH doubled the survival of CD34(+) cells and committed progenitors (CFCs) with respect to the atmospheric air (60% vs. 30%, respectively). Improved cell maintenance in HH was associated with higher proportion of aldehyde dehydrogenase (ALDH) positive cells. Cell-protective effects are associated with an improved maintenance of the plasma and mitochondrial membrane potential and with a conversion to the glycolytic energetic state. We also showed that HH decreased apoptosis, despite a sustained ROS production and a drop of ATP amount per viable cell. The proteomic study revealed that the global protein content was better preserved in HH. This analysis identified: (i) proteins sensitive or insensitive to hypothermia irrespective of the gas phase, and (ii) proteins related to the HH cell-protective effect. Among them are some protein families known to be implicated in the prolonged survival of hibernating animals in hypothermia. These findings suggest a way to optimize short-term cell conservation without freezing.

journal_name

J Cell Physiol

journal_title

Journal of cellular physiology

authors

Vlaski M,Negroni L,Kovacevic-Filipovic M,Guibert C,Brunet de la Grange P,Rossignol R,Chevaleyre J,Duchez P,Lafarge X,Praloran V,Schmitter JM,Ivanovic Z

doi

10.1002/jcp.24678

subject

Has Abstract

pub_date

2014-12-01 00:00:00

pages

2153-65

issue

12

eissn

0021-9541

issn

1097-4652

journal_volume

229

pub_type

杂志文章

相关文献

JOURNAL OF CELLULAR PHYSIOLOGY文献大全
  • Selenium deficiency in cultured adrenocortical cells: restoration of glutathione peroxidase and resistance to hydroperoxides on addition of selenium.

    abstract：:Cultured bovine adrenocortical cells were previously shown to be functionally deficient in selenium and vitamin E when grown in medium supplemented with fetal bovine serum. In the present experiments, the lack of significant bioavailable amounts of selenium in the medium was demonstrated by the finding of only low lev...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/jcp.1041230106

    authors： Hornsby PJ,Pearson DW,Autor AP,Aldern KA,Harris SE

    更新日期：1985-04-01 00:00:00

  • VASP phosphorylation at serine239 regulates the effects of NO on smooth muscle cell invasion and contraction of collagen.

    abstract：:Nitric oxide triggers cGMP-dependent kinase-mediated phosphorylation of the actin regulator vasodilator-stimulated phosphoprotein (VASP) at residue serine239. The function of this phosphorylation for smooth muscle cell (SMC) adhesion, spreading, matrix contraction, and invasion is not well understood. We reconstituted...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/jcp.21942

    authors： Defawe OD,Kim S,Chen L,Huang D,Kenagy RD,Renné T,Walter U,Daum G,Clowes AW

    更新日期：2010-01-01 00:00:00

  • Antiproliferative effects of heparin on vascular smooth muscle cells are reversed by epidermal growth factor.

    abstract：:Heparin and related glycosaminoglycans are potent inhibitors of both in vivo and in vitro smooth muscle cell (SMC) proliferation. We have found that epidermal growth factor (EGF) reverses the antiproliferative effects of heparin. Other known SMC mitogens, including platelet-derived growth factor (PDGF), insulin-like g...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/jcp.1041310203

    authors： Reilly CF,Fritze LM,Rosenberg RD

    更新日期：1987-05-01 00:00:00

  • Insulin-like growth factor binding protein (IGFBP)-5 is upregulated during both differentiation and apoptosis in primary cultures of mouse mammary epithelial cells.

    abstract：:We have previously demonstrated that insulin-like growth factor binding protein-5 (IGFBP-5) is upregulated following treatment of the mouse mammary epithelial cell line HC11 with lactogenic hormones (dexamethasone, insulin, and prolactin-DIP). In addition, we have also shown that IGFBP-5 is upregulated in mammary epit...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/jcp.20587

    authors： Lochrie JD,Phillips K,Tonner E,Flint DJ,Allan GJ,Price NC,Beattie J

    更新日期：2006-05-01 00:00:00

  • Lipid A, the active part of bacterial endotoxins in inducing serum colony stimulating activity and proliferation of splenic granulocyte/macrophage progenitor cells.

    abstract：:An analysis of which component of lipopolysaccharides (LPS), the lipid or the polysaccharide (PS), is active in stimulating the murine granulopoietic system has been performed. LPS with different structures, isolated from different mutant strains of Salmonella and chemical degradation products of lipopolysaccharides h...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/jcp.1040870110

    authors： Apte RN,Galanos C,Pluznik DH

    更新日期：1976-01-01 00:00:00

  • MiR-21 upregulation increases IL-8 expression and tumorigenesis program in airway epithelial cells exposed to cigarette smoke.

    abstract：BACKGROUND:Cigarette smoke exposure, increasing Toll-like receptor 4 (TLR4) and reactive oxygen species (ROS), promotes inflammatory responses in airway epithelial cells. Chronic inflammation, microRNA (miRNA), and oxidative stress are associated with cancer development. AIMS:The present study was aimed to explore whe...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/jcp.28786

    authors： Pace E,Di Vincenzo S,Di Salvo E,Genovese S,Dino P,Sangiorgi C,Ferraro M,Gangemi S

    更新日期：2019-12-01 00:00:00

  • Differential effects of N-TiO2 nanoparticle and its photo-activated form on autophagy and necroptosis in human melanoma A375 cells.

    abstract：:The manipulation of autophagy provides a new opportunity for highly effective anticancer therapies. Recently, we showed that photodynamic therapy (PDT) with nitrogen-doped titanium dioxide (N-TiO2 ) nanoparticles (NPs) could promote the reactive oxygen species (ROS)-dependent autophagy in leukemia cells. However, the ...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/jcp.29479

    authors： Mohammadalipour Z,Rahmati M,Khataee A,Moosavi MA

    更新日期：2020-11-01 00:00:00

  • Membrane bound and cellular cationic changes associated with insulin stimulation of cultured cells.

    abstract：:Insulin was employed as a stimulant in our continuing investigations of the molecular mechanisms involved in the coordinate control of cellular metabolism and growth. Incubation of chicken embryo fibroblasts for 16 hours in media containing 0-0.1 U insulin/ml resulted in a 17-fold increase in the rate of 3H-thymidine ...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/jcp.1040960302

    authors： Sanui H,Rubin AH

    更新日期：1978-09-01 00:00:00

  • A calcium requirement for movement of cultured cells.

    abstract：:When calcium is removed from culture medium, motility of cultured cells is decreased. The effect is rapid, reversible and pronounced. Decreased motility is observed with normal mouse Balb/c 3T3 cells, mouse L929 cells, rat kidney fibroblasts and chick embryo fibroblasts. The calcium dependence of movement can be obser...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/jcp.1041010112

    authors： Moore L,Pastan I

    更新日期：1979-10-01 00:00:00

  • A novel role of VEPH1 in regulating AoSMC phenotypic switching.

    abstract：:Abdominal aortic aneurysm (AAA) is a potentially lethal disease featured by focal dilatation in the aorta. The transition of vascular smooth muscle cells (SMCs) from a contractile/differentiated to a synthetic/dedifferentiated phenotype is considered to contribute to AAA formation and expansion. Our previous gene micr...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/jcp.29736

    authors： Shi X,Xu C,Li Y,Wang H,Ma W,Tian Y,Yang H,Li L

    更新日期：2020-12-01 00:00:00

  • Bafilomycin A1 inhibits lysosomal, phagosomal, and plasma membrane H(+)-ATPase and induces lysosomal enzyme secretion in macrophages.

    abstract：:Bafilomycin A1, a specific inhibitor of H(+)-ATPases of the vacuolar type, was in the present study shown, at similar concentrations, to induce secretion of lysosomal enzyme and to elevate lysosomal pH in mouse macrophages. These results lend support to the previous suggestion of a triggering role for an increase in l...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/jcp.1041630116

    authors： Tapper H,Sundler R

    更新日期：1995-04-01 00:00:00

  • The non-genomic effects on Na+/H+-exchange 1 by progesterone and 20alpha-hydroxyprogesterone in human T cells.

    abstract：:Progesterone is an endogenous immunomodulator and can suppress T-cell activation during pregnancy. We have previously shown that the non-genomic effects of progesterone, especially acidification, are exerted via plasma membrane sites and suppress cellular genomic responses to mitogens. This study aimed to show that ac...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/jcp.20962

    authors： Chien EJ,Liao CF,Chang CP,Pu HF,Lu LM,Shie MC,Hsieh DJ,Hsu MT

    更新日期：2007-05-01 00:00:00

  • Activation of potassium channels by hypoxia and reoxygenation in the human lung adenocarcinoma cell line A549.

    abstract：:Active oxygen species are generated in cells during pathophysiologic conditions such as inflammation and postischemic reperfusion. If oxygen radical scavengers are added before reperfusion, then the magnitude of injury is reduced. We investigated whether free radicals generated following exposure to hypoxia and reoxyg...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/jcp.1041560217

    authors： Koong AC,Giaccia AJ,Hahn GM,Saad AH

    更新日期：1993-08-01 00:00:00

  • Aryl hydrocarbon receptor-mediated impairment of chondrogenesis and fracture healing by cigarette smoke and benzo(a)pyrene.

    abstract：:The clinical literature strongly suggests that bone healing in cigarette smokers is impaired. Since cigarette smoke (CS) contains numerous polycyclic aromatic hydrocarbons (PAHs), and since dioxins impair bone formation in vivo via the Aryl Hydrocarbon Receptor (AHR), we investigated the impact of PAH/AHR signaling on...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/jcp.22819

    authors： Kung MH,Yukata K,O'Keefe RJ,Zuscik MJ

    更新日期：2012-03-01 00:00:00

  • Contradistinctive growth responses of cultured rat intestinal epithelial cells to epidermal growth factor depending on cell population density.

    abstract：:The growth response of cultured rat intestinal epithelial (RIE-1) cells to epidermal growth factor (EGF) depends on the cell population density. EGF stimulated the proliferation of RIE-1 cells in dense cultures, but inhibited the proliferation of cells growing at low population densities. In contrast, insulin enhanced...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/jcp.1041290312

    authors： Blay J,Brown KD

    更新日期：1986-12-01 00:00:00

  • Pathway of glutamate oxidation and its regulation in the HuH13 line of human hepatoma cells.

    abstract：:Well-coupled mitochondria were isolated from a HuH13 line of human hepatoma cells and human liver tissue. The liver mitochondria showed a feeble glutamine oxidation activity in contrast to the hepatoma mitochondria, whereas they utilized glutamate well for the oxidative phosphorylation. In the liver mitochondria, glut...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/jcp.1041480215

    authors： Matsuno T

    更新日期：1991-08-01 00:00:00

  • DNA methylation and cancer.

    abstract：:The methylation of DNA is an epigenetic modification that can play an important role in the control of gene expression in mammalian cells. The enzyme involved in this process is DNA methyltransferase, which catalyzes the transfer of a methyl group from S-adenosyl-methionine to cytosine residues to form 5-methylcytosin...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章,评审

    doi：10.1002/(SICI)1097-4652(200005)183:2<145::AID-JCP1

    authors： Momparler RL,Bovenzi V

    更新日期：2000-05-01 00:00:00

  • Intracellular location of cysteine transport activity correlates with productive processing of antigen disulfide.

    abstract：:Activation of CD4+ T cells requires processing of exogenous protein antigens by antigen-presenting cells (APC). A macrophage hybridoma and B cell lymphoma were comparable in their ability to process hen egg lysozyme (HEL), which involves reduction of its disulfide bonds. The intracellular levels of cysteine and glutat...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/(SICI)1097-4652(199608)168:2<248::AID-JCP3

    authors： Gainey D,Short S,McCoy KL

    更新日期：1996-08-01 00:00:00

  • Clonal heterogeneity of the sensitivity of human colon carcinoma cell lines to TGE beta isoforms.

    abstract：:Spontaneously arising, TGF beta 1-resistant colonies were isolated directly from the soft agarose plates of MOSER human colon carcinoma cells grown in the presence of TGF beta 1 but in the absence of serum. The colonies were cloned by limiting dilution and screened in a monolayer proliferation assay for sensitivity to...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/jcp.1041650309

    authors： Zhou GH,Sechrist GL,Brattain MG,Mulder KM

    更新日期：1995-12-01 00:00:00

  • Agonist-evoked Ca(2+) wave progression requires Ca(2+) and IP(3).

    abstract：:Smooth muscle responds to IP(3)-generating agonists by producing Ca(2+) waves. Here, the mechanism of wave progression has been investigated in voltage-clamped single smooth muscle cells using localized photolysis of caged IP(3) and the caged Ca(2+) buffer diazo-2. Waves, evoked by the IP(3)-generating agonist carbach...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/jcp.22103

    authors： McCarron JG,Chalmers S,MacMillan D,Olson ML

    更新日期：2010-08-01 00:00:00

  • Transglutaminase activity regulates differentiation, migration and fusion of osteoclasts via affecting actin dynamics.

    abstract：:Osteoclasts, bone resorbing cells, derive from monocyte/macrophage cell lineage. Increased osteoclast activity is responsible for bone destruction in diseases such as osteoporosis, periodontitis and rheumatoid arthritis. Transglutaminases (TGs), protein crosslinking enzymes, were recently found involved in osteoclasto...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/jcp.26603

    authors： Sun H,Kaartinen MT

    更新日期：2018-09-01 00:00:00

  • Tenascin-C enhances crosstalk signaling of integrin αvβ3/PDGFR-β complex by SRC recruitment promoting PDGF-induced proliferation and migration in smooth muscle cells.

    abstract：:Migration and proliferation of smooth muscle cells (SMCs) are key events during neointimal formation in pathological conditions of vessels. Tenascin-C (TNC) is upregulated in the developing neointima of lesions. We evaluated the effects of TNC on responses of SMCs against platelet-derived growth factor (PDGF) stimulat...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/jcp.22614

    authors： Ishigaki T,Imanaka-Yoshida K,Shimojo N,Matsushima S,Taki W,Yoshida T

    更新日期：2011-10-01 00:00:00

  • Overexpression of the type-1 insulin-like growth factor receptor increases ligand-dependent proliferation and differentiation in bovine skeletal myogenic cultures.

    abstract：:Previous studies demonstrated that overexpression of the type-1 insulin-like growth factor (IGF) receptor (IGF-1R) in skeletal myogenic cell lines increased proliferation and differentiation responses to IGF. However, it was unclear if such manipulations in primary, untransformed skeletal myogenic cells would result i...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/(SICI)1097-4652(199607)168:1<34::AID-JCP5>

    authors： Quinn LS,Haugk KL

    更新日期：1996-07-01 00:00:00

  • The long noncoding RNA ZFAS1 promotes the progression of glioma by regulating the miR-150-5p/PLP2 axis.

    abstract：:Numerous studies have reported that long noncoding RNA (lncRNA) dysregulation is involved in the progression of many malignant tumors, including glioma. The lncRNA ZNFX1 antisense RNA 1 (ZFAS1) plays an oncogenic role in various malignant tumors, such as gastric cancer and hepatocellular carcinoma. However, the underl...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/jcp.29199

    authors： Li X,Luo Y,Liu L,Cui S,Chen W,Zeng A,Shi Y,Luo L

    更新日期：2020-03-01 00:00:00

  • SP1-SYNE1-AS1-miR-525-5p feedback loop regulates Ang-II-induced cardiac hypertrophy.

    abstract：:Cardiac hypertrophy (CH) has become a huge threat to human health. Recent years, long noncoding RNAs (lncRNAs) have been studied in human diseases, including CH. According to bioinformatics analysis, 10 lncRNAs possibly involved in the progression of CH were screened out. Among which, lncRNA SYNE1 antisense RNA 1 (SYN...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/jcp.28131

    authors： Wang Y,Cao R,Yang W,Qi B

    更新日期：2019-08-01 00:00:00

  • Archetypal autophagic players through new lenses for bone marrow stem/mature cells regulation.

    abstract：:The bone marrow landscape consists of specialized and stem/progenitor cells, which coordinate important tissue-related and systemic physiological features. Within the marrow cavity, stem/progenitor and differentiated hematopoietic and skeletal cells congregate into dynamic functional assemblies throughout specific ana...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章,评审

    doi：10.1002/jcp.30296

    authors： Agas D,Sabbieti MG

    更新日期：2021-01-25 00:00:00

  • PACAP and PAC1R are differentially expressed in motor cortex of amyotrophic lateral sclerosis patients and support survival of iPSC-derived motor neurons.

    abstract：:Amyotrophic lateral sclerosis (ALS) is a fatal and disabling neurodegenerative disease characterized by upper and lower motor neurons depletion. In our previous work, comprehensive genomic profiling of 41 motor cortex samples enabled to discriminate controls from sporadic ALS patients, and segregated these latter into...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/jcp.26182

    authors： Bonaventura G,Iemmolo R,D'Amico AG,La Cognata V,Costanzo E,Zappia M,D'Agata V,Conforti FL,Aronica E,Cavallaro S

    更新日期：2018-04-01 00:00:00

  • An Innovative Assay for the Analysis of In Vitro Endothelial Remodeling: Experimental and Computational Evidence.

    abstract：:The molecular and cellular mechanisms underlying vascular remodeling are currently investigated by experimental strategies which aim to mimic the complex environmental conditions found in vivo. Some of them focus on the tubulogenic activity of dispersed endothelial cell populations, while others evaluate vascular spro...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/jcp.25468

    authors： Scianna M,Bassino E,Munaron L

    更新日期：2017-02-01 00:00:00

  • Pp60c-src mediates ERK activation/nuclear localization and PAI-1 gene expression in response to cellular deformation.

    abstract：:Release of transcellular tension upon disruption of actin stress fibers with cytochalasin D (CD) and associated changes in cell morphology are reflected in the rapid transcription of "deformation-responsive" genes. For certain genes (e.g., urokinase plasminogen activator and its type-1 inhibitor PAI-1), de novo mRNA s...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/jcp.10247

    authors： Samarakoon R,Higgins PJ

    更新日期：2003-06-01 00:00:00

  • Effects of macrophages and CXCR2 on adipogenic differentiation of bone marrow mesenchymal stem cells.

    abstract：:Macrophages and many chemokines are closely associated with the adipogenic differentiation of bone marrow mesenchymal stem cells (MSCs), but their roles in adipogenesis and the underlying mechanisms are not fully understood. Here, we first investigated the influence of macrophages on the differentiation of MSCs in vit...

    journal_title：Journal of cellular physiology

    pub_type： 杂志文章

    doi：10.1002/jcp.27634

    authors： Cao D,Ma F,Ouyang S,Liu Z,Li Y,Wu J

    更新日期：2019-06-01 00:00:00