Abstract：
:Insulin was employed as a stimulant in our continuing investigations of the molecular mechanisms involved in the coordinate control of cellular metabolism and growth. Incubation of chicken embryo fibroblasts for 16 hours in media containing 0-0.1 U insulin/ml resulted in a 17-fold increase in the rate of 3H-thymidine incorporation into DNA. Concomitantly, there were graded increases in intracellular K+ (14%) AND Mg2+ (22%) and no significant change in Ca2+. These changes in cation content occurred within 10 to 30 minutes and preceded the changes in 3H-thymidine incorporation. Insulin produced a consistent graded decrease in externally bound Mg2+ and Ca2+ and a concomitant increase in bound Na+ and K+ with no significant change in the rates of K+ and Mg2+ efflux. The results are consistent with the concept of Mg2+ as a second messenger for insulin action, as well as with the more general hypothesis that Mg2+ is the centtral agent in the coordinate control of metabolism and growth in animal cells.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Sanui H,Rubin AHdoi
10.1002/jcp.1040960302subject
Has Abstractpub_date
1978-09-01 00:00:00pages
265-78issue
3eissn
0021-9541issn
1097-4652journal_volume
96pub_type
杂志文章abstract：:A method, based on the differing capacities of cells to adhere to a column of polyester fibres, has been described for separating human bone marrow cells into a nonadherent and an adherent fraction. The effect of this cell separation procedure on colony formation by erythroid progenitor cells was investigated. In cont...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040910108
更新日期：1977-04-01 00:00:00
abstract：:In order to promote cell attachment, fibronectin must first undergo activation by a suitable substrate. In this study, 52 materials have been surveyed for their ability a) to bind fibronectin, b) to activate the cell-adhesive property of fibronectin, and c) to support the growth of cells. Many plastics, polysaccharide...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041090314
更新日期：1981-12-01 00:00:00
abstract：:The present study extends our previous observation (Kasambalides and Lanks, J. Cell. Physiol., 114:93-98, 1983), that dexamethasone inhibits the alterations in heat shock protein (HSP) and glucose-regulated protein (GRP) synthesis caused by glucose deprivation. We now show that insulin, even in the presence of high ex...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041230220
更新日期：1985-05-01 00:00:00
abstract：:The bone marrow landscape consists of specialized and stem/progenitor cells, which coordinate important tissue-related and systemic physiological features. Within the marrow cavity, stem/progenitor and differentiated hematopoietic and skeletal cells congregate into dynamic functional assemblies throughout specific ana...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.30296
更新日期：2021-01-25 00:00:00
abstract：:Radioiodinated granulocyte-macrophage colony-stimulating factor (125I-GM-CSF) binds to specific receptors (molecular weight approximately 50,000 daltons) on the murine myelomonocytic leukemia, WEHI-3BD+. At 4 degrees C 125I-GM-CSF remains on the surface of the cells and can be eluted by washing the cells with acidifie...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041300211
更新日期：1987-02-01 00:00:00
abstract：:Bioinformatics is an interdisciplinary field that blends computer science and biostatistics with biological and biomedical sciences such as biochemistry, cell biology, developmental biology, genetics, genomics, and physiology. An important goal of bioinformatics is to facilitate the management, analysis, and interpret...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.21218
更新日期：2007-11-01 00:00:00
abstract：:Ion channels are pivotal to many aspects of sperm physiology and function. We have used the patch clamp technique to investigate the distribution of ion channels in the plasma membrane of the head of human spermatozoa. We report that three types of activity are common in the equatorial and acrosomal regions of the spe...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21153
更新日期：2007-12-01 00:00:00
abstract：:As a natural metabolite of limonoids from Dictamnus dasycarpus, fraxinellone has been reported to be neuroprotective and anti-inflammatory. However, its influence on cellular metabolism remains largely unknown. In the present study, we investigated the effect of fraxinellone on cellular senescence-induced by oxidative...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.26169
更新日期：2018-05-01 00:00:00
abstract：:Long noncoding RNAs have been reported to be important regulators in numerous cancers. In this study, we found that HOXC13 antisense RNA (HOXC13-AS) was highly expressed in head and neck squamous carcinoma (HNSC) tissues in The Cancer Genome Atlas database. Nasopharyngeal carcinoma (NPC) belongs to HNSC. Therefore, we...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27915
更新日期：2019-08-01 00:00:00
abstract：:Bafilomycin A1, a specific inhibitor of H(+)-ATPases of the vacuolar type, was in the present study shown, at similar concentrations, to induce secretion of lysosomal enzyme and to elevate lysosomal pH in mouse macrophages. These results lend support to the previous suggestion of a triggering role for an increase in l...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041630116
更新日期：1995-04-01 00:00:00
abstract：:Rat embryo fibroblasts were grown in medium containing 14C-leucine and 3H-thymidine. After a 24-hour chase in nonlabeled medium, cultures were placed in either fresh growth medium or medium containing 10-20 microgram/ml cycloheximide. Cell monolayers were processed at daily intervals for three days. Four hours prior t...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040970302
更新日期：1978-12-01 00:00:00
abstract：:The role of microtubules in mitogen-induced human lymphocyte activation and proliferation was examined. The effect of colchicine, a microtubule-disrupting agent, was compared with taxol, a microtubule-stabilizing drug, and with isaxonine (N-isopropyl-amino-2-pyrimidine orthophosphate), a proposed microtubular-active d...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041160202
更新日期：1983-08-01 00:00:00
abstract：:We have previously reported that the proliferation of cloned MCF-7 and T47D human mammary tumor cells can be inhibited by increasing concentrations of charcoal-dextran stripped female human serum (CDFHS). The maximal proliferation rate was restored by the addition of 3 X 10(-11) M estradiol-17 beta to the culture medi...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041240305
更新日期：1985-09-01 00:00:00
abstract：:The poor prognosis of pancreatic cancer patients using currently available therapies mandates novel therapeutics that combine anti-neoplastic potency with toxicity-minimizing cancer specificity. Employing an overlapping pathway screen to identify genes exhibiting coordinated expression as a consequence of terminal cel...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21320
更新日期：2008-05-01 00:00:00
abstract：:Exosomes extracted from mesenchymal stem cells (MSCs) was reported to reduce myocardial ischemia/reperfusion damage. Besides, stromal-derived factor 1 (SDF1a) functions as cardiac repair after myocardial infarction (MI). Therefore, the present study aims to identify whether exosomes (Exo) released from SDF1-overexpres...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28070
更新日期：2019-08-01 00:00:00
abstract：:Dental follicle cells (DFCs) activate and recruit osteoclasts for tooth development and tooth eruption, whereas DFCs themselves differentiate into osteoblasts to form alveolar bone surrounding tooth roots through the interaction with Hertwig's epithelial root sheath (HERS). Also during tooth development, parathyroid h...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27857
更新日期：2019-07-01 00:00:00
abstract：:With the long-range goals of elucidating the biochemical genetics and the phenotypic expression, both in vitro and in vivo, of mutator strains of mammalian cells, we have isolated and partially characterized a series of drug-resistant mutants from a subline a feeder-dependent parental mouse teratocarcinoma line (PSA-1...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041150103
更新日期：1983-04-01 00:00:00
abstract：:The biosynthesis and assembly of the peripheral sector (V1) of the vacuolar proton-translocating adenosine triphosphatase (V-ATPase) was studied in a bovine kidney epithelial cell line. Monolayer cultures of cells were metabolically radiolabeled with Tran35S-label and the V-ATPase subsequently immunoprecipitated using...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041560106
更新日期：1993-07-01 00:00:00
abstract：:Vitamin C is an essential micronutrient for the development of male germ cells. In the gonad, the germ cells are isolated from the systemic circulation by the blood-testis barrier, which consists of a basal layer of Sertoli cells that communicate through an extensive array of tight junction complexes. To study the beh...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21545
更新日期：2008-12-01 00:00:00
abstract：:Bcl-2 associated athanogene 5 (Bag5) is a novel endoplasmic reticulum (ER) regulator. However, its role in catecholamine-induced endothelial cells damage has not been fully understood. In our study, catecholamine was used to mimic hypertension-related endothelial cell damage. Then, western blots, enzyme-linked immunos...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29904
更新日期：2021-02-01 00:00:00
abstract：BACKGROUND:Surgical stress has been suggested to facilitate colon cancer growth and metastasis. However, the precise mechanisms by which surgical trauma promotes colon cancer progression remain poorly understood. METHODS:To unravel the mechanisms underlying surgery-induced colon cancer progression, a syngenic transpla...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27245
更新日期：2019-04-01 00:00:00
abstract：:Glucose deprivation, a pathophysiological cell condition, causes up-regulation of GRP78 and induction of etoposide resistance in human cancer cells. The induction of drug resistance can be partly explained by the fact that GRP78 can block activation of caspase-7 induced by treatment with etoposide. Therefore, downregu...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21308
更新日期：2008-04-01 00:00:00
abstract：:Cancer is one of the common lifestyle diseases and is considered to be the leading cause of death worldwide. Epstein-Barr virus (EBV)-infected individuals remain asymptomatic; but under certain stress conditions, EBV may lead to the development of cancers such as Burkitt's and Hodgkin's lymphoma and nasopharyngeal car...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27380
更新日期：2019-05-01 00:00:00
abstract：:Testicular cancer is the most common solid malignancy among young men. We downloaded data of testicular cancer patients from The Cancer Genome Atlas database to find novel genes in the testicular cancer microenviroment based on ESTIMATE algorithm-derived immune scores. A total of 156 cases of testicular cancer were in...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29898
更新日期：2021-01-01 00:00:00
abstract：:We investigated the regulation of Cl- secretion by adrenoceptors in polarized 16HBE14o- human bronchial epithelial cells. Treatment with the nonselective β adrenoceptor agonist isoprenaline stimulated an increase in short-circuit current (ISC ), which was inhibited by the β adrenoceptor blocker propranolol. Treatment ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29683
更新日期：2020-11-01 00:00:00
abstract：:We have previously shown that extracellular ATP acts as a mitogen via protein kinase C (PKC)-dependent and independent pathways (Wang, D., Huang, N., Gonzalez, F.A., and Heppel, L.A. Multiple signal transduction pathways lead to extracellular ATP-stimulated mitogenesis in mammalian cells. I. Involvement of protein kin...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041460320
更新日期：1991-03-01 00:00:00
abstract：:Synovial fibroblasts (SFs) of rheumatoid arthritis (RA) are phenotypically aggressive, typically progressing into arthritic cartilage degradation. Throughout our study, we made explorations into the effects of microRNA-135a (miR-135a) on the SFs involved in RA by mediating the phosphatidylinositol 3-kinase (PI3K)/AKT ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28390
更新日期：2019-08-01 00:00:00
abstract：:In the presence of lovastatin (mevinolin), an inhibitor of endogenous mevalonate synthesis, C1300 murine neuroblastoma cells incorporated (2-14C)mevalonate into several discrete polypeptides that were separable by SDS-PAGE. The electrophoretic pattern of the labeled proteins did not vary substantially when cells were ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041330307
更新日期：1987-12-01 00:00:00
abstract：:Colon cancer stem cells (CCSCs) stand for a critical subpopulation of colon cancer cells that possess self-renewal and multilineage differentiation potentials and drive tumorigenicity. Due to their impact on treatment tolerance, CCSCs have been a hot research topic in the past few years. We have previously reported th...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28836
更新日期：2019-12-01 00:00:00
abstract：:The advent of high throughput gene expression profiling, from microarrays to sequence based assays has yielded vast insight into the biology of tumors. New technologies are constantly being unveiled which promise to generate more accurate maps of tumor gene deregulation, and demand the development of new strategies in...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.21554
更新日期：2008-12-01 00:00:00
