Abstract:
:The role of microtubules in mitogen-induced human lymphocyte activation and proliferation was examined. The effect of colchicine, a microtubule-disrupting agent, was compared with taxol, a microtubule-stabilizing drug, and with isaxonine (N-isopropyl-amino-2-pyrimidine orthophosphate), a proposed microtubular-active drug. Lymphocyte proliferation, assessed by measuring the increase in the number of cells in mitogen-stimulated cultures, was completely suppressed by both colchicine and taxol (100 nM) whereas significant inhibition by isaxonine required much higher concentrations (5 mM). In order to characterize the inhibition, initial lymphocyte blast transformation and subsequent DNA synthesis were investigated. Neither colchicine nor taxol inhibited lymphocyte blast transformation assessed by quantitating the change in volume of the stimulated cells after a 24-hour incubation. In contrast, isaxonine (2-5 mM) suppressed blast transformation. Initial DNA synthesis, evaluated by measuring the cumulative incorporation of [3H]thymidine between 30 and 48 hours of culture, was inhibited in a concentration-dependent manner by both isaxonine and colchicine but not by taxol. Electron microscopic studies confirmed that both taxol and colchicine (10 nM) arrested the responding lymphocytes in mitosis, and that isaxonine inhibited initial activation. These results suggest that normal microtubule function is only necessary for cell division and that drug effects on blast transformation and initial DNA synthesis are unrelated to microtubules.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Cuthbert JA,Shay JWdoi
10.1002/jcp.1041160202subject
Has Abstractpub_date
1983-08-01 00:00:00pages
127-34issue
2eissn
0021-9541issn
1097-4652journal_volume
116pub_type
杂志文章abstract::Exosomes extracted from mesenchymal stem cells (MSCs) was reported to reduce myocardial ischemia/reperfusion damage. Besides, stromal-derived factor 1 (SDF1a) functions as cardiac repair after myocardial infarction (MI). Therefore, the present study aims to identify whether exosomes (Exo) released from SDF1-overexpres...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
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更新日期:2019-08-01 00:00:00
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journal_title:Journal of cellular physiology
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journal_title:Journal of cellular physiology
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journal_title:Journal of cellular physiology
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更新日期:2014-09-01 00:00:00
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journal_title:Journal of cellular physiology
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更新日期:2020-11-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041080104
更新日期:1981-07-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041410126
更新日期:1989-10-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041400302
更新日期:1989-09-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199812)177:4<575::AID-JCP8
更新日期:1998-12-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041560222
更新日期:1993-08-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041090105
更新日期:1981-10-01 00:00:00
abstract::MicroRNA 520c and 373 (miR-520c and miR-373) have been characterized as oncogenes and play critical roles in cancer cell metastasis. However, the relationship between these two microRNAs and matrix metalloproteinases (MMPs), which are important in cancer cell metastasis, remains unknown. Here, we report new evidence i...
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更新日期:2012-02-01 00:00:00
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journal_title:Journal of cellular physiology
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更新日期:2005-09-01 00:00:00
abstract::In culture, B16/C3 murine melanoma cells grown in the presence of serum undergo melanogenesis at a specific time after plating. At this time, melanin is synthesized intracellularly and then secreted into the extracellular culture fluid. We have found that melanin secretion is dependent on the presence of serum in the ...
journal_title:Journal of cellular physiology
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更新日期:1982-12-01 00:00:00
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更新日期:2012-01-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199803)174:3<285::AID-JCP2
更新日期:1998-03-01 00:00:00
abstract::There is compelling in vitro and in vivo evidence to implicate mast cells in the development of fibrosis. However, an important question remains as to the mechanisms by which mast cells mediate fibrosis. Recent evidence from our laboratory (Gruber et al., 1997, J. Immunol. , 158:2310-2317) has revealed that tryptase, ...
journal_title:Journal of cellular physiology
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更新日期:1999-11-01 00:00:00
abstract::We have previously shown that retinoic acid (RA) has protective effects on high glucose (HG)-induced cardiomyocyte apoptosis. To further elucidate the molecular mechanisms of RA effects, we determined the interaction between nuclear factor (NF)-κB and RA signaling. HG induced a sustained phosphorylation of IKK/IκBα an...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
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更新日期:2013-02-01 00:00:00
abstract::The physiological approach suggests that an environment associating the mesenchymal stromal cells (MSC) and low O(2) concentration would be most favorable for the maintenance of hematopoietic stem cells (HSCs) in course of ex vivo expansion of hematopoietic grafts. To test this hypothesis, we performed a co-culture of...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
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更新日期:2012-06-01 00:00:00
abstract::Transforming growth factor-beta1 (TGF-beta1) stimulates expression of plasminogen activator inhibitor type-1 (PAI-1), a serine protease inhibitor (SERPIN) important in the control of stromal barrier proteolysis and cell-to-matrix adhesion. Pharmacologic agents that target MEK (PD98059, U0126) or src family (PP1) kinas...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20279
更新日期:2005-07-01 00:00:00
abstract::Transient Receptor Potential Canonical (TRPC) channels are implicated in modulating neurite outgrowth. The expression pattern of TRPCs changes significantly during brain development, suggesting that fine-tuning TRPC expression may be important for orchestrating neuritogenesis. To study how alterations in the TRPC expr...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22855
更新日期:2012-04-01 00:00:00
abstract::In vitro generation of HSCs from pluripotent stem cells (PSCs) can be regarded as novel therapeutic approaches for replacing bone marrow (BM) transplantation without immune rejection or graft versus host disease(GVHD). To date, many differentiation approaches have been evaluated in terms of directing PSCs toward diffe...
journal_title:Journal of cellular physiology
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更新日期:2018-01-31 00:00:00
abstract::Peroxisome proliferator activated receptors (PPARs, alpha, beta/delta, gamma) control lipid homeostasis and differentiation in various tissues and tumor cells. PPARbeta and PPARgamma increase oligodendrocyte maturation in glial mixed populations and spinal cord oligodendrocytes, respectively, and PPARbeta is known to ...
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pub_type: 杂志文章
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更新日期:2008-11-01 00:00:00