Abstract:
:Fas, which functions to initiate a signal causing apoptosis, is expressed in epithelia, thus, suggesting a role in controlling cell number during states of cell and matrix turnover. In view of this, we hypothesized that cell-matrix interactions may be an important determinant of Fas expression in epithelial cells. To investigate this, we examined the effect of insoluble extracellular matrix molecules on Fas expression in murine lung epithelial (MLE) cells, a transformed mouse lung epithelial cell line. We report that 1) insoluble extracellular matrices increased Fas mRNA in a time and concentration-dependent manner; 2) induced increases in Fas mRNA were associated with concomitantly increased Fas protein; and 3) nonspecific adherence to a polylysine substrate did not induce Fas mRNA. Consistent with these findings, Fas-induced apoptosis was significantly enhanced in cultures plated on type IV collagen. Employing rat hepatocytes, we confirmed that the insoluble extracellular matrix also increases Fas expression in primary epithelial cells. By amplifying Fas-mediated apoptosis, these data suggest a mechanism whereby the extracellular matrix regulates the fate of specific epithelial cell populations.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Fine A,Miranda K,Farmer SR,Anderson NLdoi
10.1002/(SICI)1097-4652(199803)174:3<285::AID-JCP2subject
Has Abstractpub_date
1998-03-01 00:00:00pages
285-92issue
3eissn
0021-9541issn
1097-4652pii
10.1002/(SICI)1097-4652(199803)174:3<285::AID-JCP2journal_volume
174pub_type
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