Role of RB and RB2/P130 genes in marrow stromal stem cells plasticity.


:Marrow stromal cells (MSCs) are stem-like cells having a striking somatic plasticity. In fact, besides differentiating into mesenchymal lineages (bone, cartilage, and fat), they are capable of differentiating into neurons and astrocytes in vitro and in vivo. The RB and RB2/P130 genes, belonging to the retinoblastoma gene family, play a key role in neurogenesis, and for this reason, we investigated their role in neural commitment and differentiation of MSCs. In MSCs that were either uncommitted or committed toward neural differentiation, we ectopically expressed RB and RB2/P130 genes and analyzed their role in regulating the cell cycle, apoptosis and differentiation. In uncommitted MSCs, the activity of RB and RB2/P130 appeared limited to negatively regulating cell cycle progression, having no role in apoptosis and differentiation (toward either mesenchymal or neural lineages). On the other hand, in MSCs committed toward the neural phenotype, both RB and RB2/P130 reduced cell proliferation rate and affected the apoptotic process. RB protected differentiating cells from programmed cell death. On the contrary, RB2/P130 increased the percentage of cells in apoptosis. All of these activities were accomplished mainly in an HDAC-independent way. The retinoblastoma genes also influenced differentiation in neural committed MSCs. RB2/P130 contributes mainly to the induction of generic neural properties, while RB triggers cholinergic differentiation. These differentiating activities are HDAC-dependent. Our research shows that there is a critical temporal requirement for the RB genes during neuronal differentiation of MSCs: they are not required for cell commitment but play a role in the maturation process. For the above reasons, RB and RB2/P130 may have a role in neural differentiation but not in neural determination.


J Cell Physiol


Jori FP,Napolitano MA,Melone MA,Cipollaro M,Cascino A,Giordano A,Galderisi U





Has Abstract


2004-08-01 00:00:00












  • A novel strategy of identifying circRNA biomarkers in cardiovascular disease by meta-analysis.

    abstract::Circular RNAs (circRNAs) are stable and abundantly expressed in vivo but are abnormally expressed in several diseases. This study aimed to identify circRNAs acting as potential biomarkers for cardiovascular disease (CVD). Research were retrieved from the articles published by September 2018 in eight databases to compa...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,meta分析,评审


    authors: Li JJ,Wang W,Wang XQ,He Y,Wang SS,Yan YX

    更新日期:2019-12-01 00:00:00

  • Human mesenchymal stem cells: a bank perspective on the isolation, characterization and potential of alternative sources for the regeneration of musculoskeletal tissues.

    abstract::The continuous discovery of human mesenchymal stem cells (hMSCs) in different tissues is stirring up a tremendous interest as a cell source for regenerative medicine therapies. Historically, hMSCs have been always considered a sub-population of mononuclear cells present in the bone marrow (BM). Although BM-hMSCs are s...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Moroni L,Fornasari PM

    更新日期:2013-04-01 00:00:00

  • Versatile role of curcumin and its derivatives in lung cancer therapy.

    abstract::Lung cancer is a main cause of death all over the world with a high incidence rate. Metastasis into neighboring and distant tissues as well as resistance of cancer cells to chemotherapy demand novel strategies in lung cancer therapy. Curcumin is a naturally occurring nutraceutical compound derived from Curcuma longa (...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Ashrafizadeh M,Najafi M,Makvandi P,Zarrabi A,Farkhondeh T,Samarghandian S

    更新日期:2020-12-01 00:00:00

  • AMD3100 inhibits epithelial-mesenchymal transition, cell invasion, and metastasis in the liver and the lung through blocking the SDF-1α/CXCR4 signaling pathway in prostate cancer.

    abstract::Stromal cell-derived factor-1 (SDF-1) and CXC chemokine receptor 4 (CXCR4) have been found to be tightly correlated with the progression of prostate cancer (PC). In this study, we investigated the effects of an SDF-1α/CXCR4 inhibitor, AMD3100, on cell progression and metastasis potential of human PC cells. Human PC ce...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zhu WB,Zhao ZF,Zhou X

    更新日期:2019-07-01 00:00:00

  • Fibroblast-dependent growth of mouse mast cells in vitro: duplication of mast cell depletion in mutant mice of W/Wv genotype.

    abstract::In spite of the apparent depletion of mast cells in tissues of mutant mice of W/Wv genotype, cells with many features of mast cells do develop when bone marrow cells of W/Wv mice are cultured in the presence of pokeweed mitogen-stimulated spleen cell-conditioned medium (PWM-SCM). In order to resolve this discrepancy a...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Fujita J,Nakayama H,Onoue H,Kanakura Y,Nakano T,Asai H,Takeda S,Honjo T,Kitamura Y

    更新日期:1988-01-01 00:00:00

  • Toll-like receptors: A pathway alluding to cancer control.

    abstract::Toll-like receptors (TLRs) are usually expressed on immune cells such as macrophages, dendritic cells, mast cells, as well as on eosinophils and some epithelial cells. They play a central role in the recognition of harmful molecules that belong to invading microorganisms or internal damaged tissues, which lead to infl...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Ayala-Cuellar AP,Cho J,Choi KC

    更新日期:2019-12-01 00:00:00

  • CRM197 in Combination With shRNA Interference of VCAM-1 Displays Enhanced Inhibitory Effects on Human Glioblastoma Cells.

    abstract::CRM197 is a naturally nontoxic diphtheria toxin mutant that binds and inhibits heparin-binding epidermal growth factor-like growth factor. CRM197 serves as carrier protein for vaccine and other therapeutic agents. CRM197 also inhibits the growth, migration, invasion, and induces apoptosis in various tumors. Vascular c...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Hu Y,Lin X,Wang P,Xue YX,Li Z,Liu LB,Yu B,Feng TD,Liu YH

    更新日期:2015-08-01 00:00:00

  • Tfcp2l1 safeguards the maintenance of human embryonic stem cell self-renewal.

    abstract::Tfcp2l1 is a transcription factor critical for mouse embryonic stem cell (mESC) maintenance. However, its role in human ESCs (hESCs) remains unclear. Here, we investigated the role of Tfcp2l1 in controlling hESC activity and showed that Tfcp2l1 is functionally important in the maintenance of hESC identity. Tfcp2l1 exp...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Sun H,You Y,Guo M,Wang X,Zhang Y,Ye S

    更新日期:2018-09-01 00:00:00

  • β2 adrenoceptor signaling regulates ion transport in 16HBE14o- human airway epithelial cells.

    abstract::We investigated the regulation of Cl- secretion by adrenoceptors in polarized 16HBE14o- human bronchial epithelial cells. Treatment with the nonselective β adrenoceptor agonist isoprenaline stimulated an increase in short-circuit current (ISC ), which was inhibited by the β adrenoceptor blocker propranolol. Treatment ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zhang RG,Yip CY,Pan KW,Cai MY,Ko WH

    更新日期:2020-11-01 00:00:00

  • Atypical PKC-zeta and PKC-iota mediate opposing effects on MCF-7 Na+/K+ATPase activity.

    abstract::We demonstrated previously that in serum-starved MCF-7 breast cancer cell line, Ang II increased Na+/K+ATPase activity and activated the protein kinase C zeta (PKC-zeta) (Muscella et al., 2002 J Endocrinol 173:315-323; 2003 J Cell Physiol 197:61-68.). The aim of the present study was to investigate the modulation of t...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Muscella A,Storelli C,Marsigliante S

    更新日期:2005-11-01 00:00:00

  • Adenosine uptake, transport, and metabolism in human erythrocytes.

    abstract::Using rapid kinetic techniques, we have determined the kinetics of zero-trans influx and equilibrium exchange of adenosine, and its uptake and in situ phosphorylation at 25 degrees C in human erythrocytes which were pretreated with 2'-deoxycoformycin to inhibit deamination of adenosine. Both the Km and Vmax for adenos...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Plagemann PG,Wohlhueter RM,Kraupp M

    更新日期:1985-11-01 00:00:00

  • Glucose intolerance in monosodium glutamate obesity is linked to hyperglucagonemia and insulin resistance in α cells.

    abstract::Obesity predisposes to glucose intolerance and type 2 diabetes (T2D). This disease is often characterized by insulin resistance, changes in insulin clearance, and β-cell dysfunction. However, studies indicate that, for T2D development, disruptions in glucagon physiology also occur. Herein, we investigated the involvem...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Araujo TR,da Silva JA,Vettorazzi JF,Freitas IN,Lubaczeuski C,Magalhães EA,Silva JN,Ribeiro ES,Boschero AC,Carneiro EM,Bonfleur ML,Ribeiro RA

    更新日期:2019-05-01 00:00:00

  • Exosomes derived from acute myeloid leukemia cells promote chemoresistance by enhancing glycolysis-mediated vascular remodeling.

    abstract::Acute myeloid leukemia (AML) is the most common type of leukemia in adults. AML cells secrete angiogenic factors to remodel vasculature and acquire chemoresistance; however, antiangiogenic drugs are often ineffective in AML treatment. Cancer cell-derived exosomes can induce angiogenesis, but their role in vascular rem...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Wang B,Wang X,Hou D,Huang Q,Zhan W,Chen C,Liu J,You R,Xie J,Chen P,Huang H

    更新日期:2019-07-01 00:00:00

  • HER2 signaling enhances 5'UTR-mediated translation of c-Myc mRNA.

    abstract::The increased levels of c-Myc protein observed previously in an ovarian carcinoma cell line stably transfected to express HER2 has suggested a role for the HER2 pathway in c-Myc expression. Analysis of HER2-transfected cells stimulated with heregulin beta1 (HRG) revealed increased c-Myc protein levels but not a corres...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Galmozzi E,Casalini P,Iorio MV,Casati B,Olgiati C,Ménard S

    更新日期:2004-07-01 00:00:00

  • Uptake of alpha-aminoisobutyric acid and phosphate by membrane vesicles derived from growing and quiescent fibroblasts.

    abstract::Membrane vesicles derived principally from the plasma membrane and endoplasmic reticulum of mouse 3T3 cells transformed by Simian virus 40 take up alpha-aminoisobutyric acid (AIB) and phosphate (Pi). When NaCl is added simultaneously with AIB or Pi, uptake rises two- to three-times above the equilibrium to accumulate ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Nilsen-Hamilton M,Hamilton RT

    更新日期:1976-12-01 00:00:00

  • WISP1 promotes non-alcoholic fatty liver disease and skeletal muscle insulin resistance via TLR4/JNK signaling.

    abstract::Wnt1-inducible signaling pathway protein-1 (WISP1) is a Cyr61/CTGF/NOV (CCN) family matricellular protein involved in adipogenesis and low-grade inflammation in obesity. However, the roles of WISP1 in hepatic steatosis and insulin resistance in skeletal muscle remain elusive. Mouse primary hepatocytes and differentiat...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Jung TW,Kang C,Goh J,Chae SI,Kim HC,Lee TJ,Abd El-Aty AM,Jeong JH

    更新日期:2018-08-01 00:00:00

  • MALAT1 via microRNA-17 regulation of insulin transcription is involved in the dysfunction of pancreatic β-cells induced by cigarette smoke extract.

    abstract::Cigarettes contain various chemicals with the potential to influence metabolic health. Exposure to cigarette smoke causes a dysfunction in pancreatic β-cells and impairs insulin production. However, the mechanisms for cigarette smoke-induced reduction of insulin remain largely unclear. Data from 558 patients with diab...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Sun Q,Xu H,Xue J,Yang Q,Chen C,Yang P,Han A,Tu Q,Lu J,Gao X,Xiang Q,Liu Q

    更新日期:2018-11-01 00:00:00

  • Interrelationship between the Na+/glucose cotransporter and CFTR in Caco-2 cells: relevance to cystic fibrosis.

    abstract::Both the Na+-dependent glucose cotransporter (SGLT1) and the cystic fibrosis transmembrane conductance regulator (CFTR) modulate Na+ and fluid movement, although in opposite directions. Yet few studies have investigated a possible interrelationship between these two transporters. By using the Caco-2 human colon carcin...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Mailleau C,Capeau J,Brahimi-Horn MC

    更新日期:1998-09-01 00:00:00

  • Effects of temperature on the degradation and biliary secretion of asialoorosomucoid by the perfused rat liver: evidence for two intracellular pathways.

    abstract::We have utilized the in situ perfused rat liver under nonrecirculating conditions to examine the effect of temperature on the metabolism and biliary secretion of [125I]-asialoorosomucid (ASOR). In this manner we were able to follow the fate of a single round of internalized ligand. In control livers perfused at 37 deg...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Kloppel TM

    更新日期:1989-03-01 00:00:00

  • SIRT1 interacts with p73 and suppresses p73-dependent transcriptional activity.

    abstract::The tumor suppressor p53-related p73 shares significant amino-acid sequence identity with p53. Like p53, p73 recognizes canonical p53 DNA-binding sites and activates p53-responsive target genes and induces apoptosis. Moreover, SIRT1 binds to p53 while repressing the expression of their target genes. Here, we report th...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Dai JM,Wang ZY,Sun DC,Lin RX,Wang SQ

    更新日期:2007-01-01 00:00:00

  • An anti-apoptotic protein, Hax-1, inhibits the HIV-1 rev function by altering its sub-cellular localization.

    abstract::The human immunodeficiency virus type 1 (HIV-1) Rev protein facilitates the nuclear export of viral mRNA containing the Rev response element (RRE). Although several host proteins co-operating with Rev in viral RNA export have been reported, little is known about the innate host defense factors that Rev overcomes to me...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Modem S,Reddy TR

    更新日期:2008-01-01 00:00:00

  • Journal of Cellular Physiology: Volume 230, Number 2, February 2015.

    abstract::Cover: Diagram illustrating the chromatic median analysis. See article by Pliss et al. on pages 427-439. ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章



    更新日期:2015-02-01 00:00:00

  • Beta adrenergic receptor repopulation of C6 glioma cells after irreversible blockade and down regulation.

    abstract::C6 glioma cells possess beta adrenergic receptors coupled with adenylate cyclase which can be irreversibly blocked by bromoacetylaminomethylpindolol (Br-AAM-pindolol), a beta adrenergic antagonist. With 1 microM Br-AAM-pindolol, more than 80% of beta adrenergic receptors, labeled by (3H)-dihydroalprenolol [3H)-DHA), w...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Homburger V,Pantaloni C,Lucas M,Gozlan H,Bockaert J

    更新日期:1984-12-01 00:00:00

  • Rapid cell surface-related stimulation of alkaline phosphatase in HeLa cells by dimethyl DL-2,3-distearoylotypropyl-2'-hydroxyethylammonium acetate (Rosenthal's inhibitor).

    abstract::Cultures of HeLa cells, strains S3G (HeLa65) and S3K (HeLa71) were grown in plastic dishes until firmly attached and were then treated with sonic dispersions of Rosenthal's phospholipase inhibitor. A rapid increase in alkaline phosphatase activity occurred following this treatment in the S3G strain (low inducible alka...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Melnykovych G,Lopez IC

    更新日期:1977-07-01 00:00:00

  • Comparative genomics of NAD(P) biosynthesis and novel antibiotic drug targets.

    abstract::NAD(P) is an indispensable cofactor for all organisms and its biosynthetic pathways are proposed as promising novel antibiotics targets against pathogens such as Mycobacterium tuberculosis. Six NAD(P) biosynthetic pathways were reconstructed by comparative genomics: de novo pathway (Asp), de novo pathway (Try), NmR pa...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Bi J,Wang H,Xie J

    更新日期:2011-02-01 00:00:00

  • Signal transducer and activator of transcription 3 mediates apoptosis inhibition through reducing mitochondrial ROS and activating Bcl-2 in gemcitabine-resistant lung cancer A549 cells.

    abstract::Lung cancer is a leading cause of cancer-related death worldwide. In this study, we used lung adenocarcinoma cells as a model, as lung adenocarcinoma has the highest mortality rate among all lung cancers. For the past few years, medical treatments or lung cancer have been limited because of chemotherapy resistance. Th...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Liu JS,Yeh CA,Huang IC,Huang GY,Chiu CH,Mahalakshmi B,Wen SY,Huang CY,Kuo WW

    更新日期:2020-12-07 00:00:00

  • Transforming activity of mutant human p53 alleles.

    abstract::Mutant forms of the p53 gene have been shown to cooperate with an activated ras gene in transforming primary cells in culture. The aberrant proteins encoded by p53 mutants are thought to act in a dominant negative manner in these assays. In vivo data, however, reveal that where p53 has undergone genetic change in tumo...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Slingerland JM,Benchimol S

    更新日期:1991-09-01 00:00:00

  • Cdk5 mediates vimentin Ser56 phosphorylation during GTP-induced secretion by neutrophils.

    abstract::Secretion by neutrophils contributes to acute inflammation following injury or infection. Vimentin has been shown to be important for secretion by neutrophils but little is known about its dynamics during secretion, which is regulated by cyclin-dependent kinase 5 (Cdk5). In this study, we sought to examine the vimenti...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Lee KY,Liu L,Jin Y,Fu SB,Rosales JL

    更新日期:2012-02-01 00:00:00

  • Caffeine induces G2/M arrest and apoptosis via a novel p53-dependent pathway in NB4 promyelocytic leukemia cells.

    abstract::Methylxantine derivative, caffeine, is known to prevent the p53-dependent apoptosis pathway via inhibition of ATM (ataxia telangiectasia mutated) kinase, which activates p53 by phosphorylation of the Ser-15 residue. In contrast, it has been reported that caffeine induces p53-mediated apoptosis through Bax protein in n...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ito K,Nakazato T,Miyakawa Y,Yamato K,Ikeda Y,Kizaki M

    更新日期:2003-08-01 00:00:00

  • Effects of Helicobacter pylori infection on cell cycle progression and the expression of cell cycle regulatory proteins.

    abstract::Helicobacter pylori lives in the stomach lumen adhering and specifically interacting with gastric epithelial cells. H. pylori infection can cause a broad range of diseases. Although most infected individuals only develop a chronic inflammation of the stomach, some patients progress to chronic gastritis, duodenal ulcer...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: De Luca A,De Falco M,Iaquinto S,Iaquinto G

    更新日期:2004-09-01 00:00:00