SIRT6 overexpression inhibits cementogenesis by suppressing glucose transporter 1.


:Cementum, which shares common features with bone in terms of biochemical composition, is important for the homeostasis of periodontium during periodontitis and orthodontic treatment. Sirtuin 6 (SIRT6), as a member of the sirtuin family, plays key roles in the osteogenic differentiation of bone marrow mesenchymal stem cells. However, the involvement of SIRT6 in cementoblast differentiation and mineralization and the underlying mechanisms remain unknown. In this study, we observed that the expression of SIRT6 increased during cementoblast differentiation initially. Analysis of the gain- and loss-of-function indicated that overexpressing SIRT6 in OCCM-30 cells suppresses cementoblast differentiation and mineralization and downregulating SIRT6 promotes cementogenesis. GLUT1, a glucose transporter necessary in cementogenesis, was inhibited by SIRT6. Overexpressing GLUT1 in SIRT6-overexpressed OCCM-30 cells rescued the inhibitory effect of SIRT6 on cementoblast differentiation and mineralization. Moreover, AMPK was activated after overexpressing SIRT6 and inhibited cementoblast differentiation and mineralization. Downregulating the expression of SIRT6 inhibited AMPK activity. Meanwhile, GLUT1 overexpression significantly decreased AMPK activity. Overall, on one hand, SIRT6 inhibited cementoblast differentiation and mineralization by suppressing GLUT1. On the other hand, SIRT6 inhibited cementoblast differentiation and mineralization by activating the AMPK pathway. GLUT1 overexpression also rescued the increased AMPK pathway activated by SIRT6.


J Cell Physiol


Huang L,Sun H,Song F,Cao Z,Jiang X,Zhang L,Li Z,Huang C




Has Abstract


2019-04-01 00:00:00












  • Urinary 8-OHdG elevations in a partial lesion rat model of Parkinson's disease correlate with behavioral symptoms and nigrostriatal dopaminergic depletion.

    abstract::Increased oxidative stress contributes to pathogenesis of Parkinson's disease (PD). 8-hydroxy-2'-deoxyguanosine (8-OHdG) is the oxidation product most frequently measured as an indicator of oxidative DNA damage. Several studies have shown increased 8-OHdG in PD patients. There are few basic laboratory data examining 8...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Kikuchi Y,Yasuhara T,Agari T,Kondo A,Kuramoto S,Kameda M,Kadota T,Baba T,Tajiri N,Wang F,Tayra JT,Liang H,Miyoshi Y,Borlongan CV,Date I

    更新日期:2011-05-01 00:00:00

  • Endothelial cell injury in vitro is associated with increased secretion of an Mr 43,000 glycoprotein ligand.

    abstract::A novel, serum albumin-binding glycoprotein of molecular weight (mw) 43,000 (43K protein) was initially purified from the culture medium of bovine aortic endothelial (BAE) cells (Sage, H., Johnson, C., and Bornstein, P., J. Biol. Chem. 259:3993-4007, 1984). Its secretion by normal mesenchymal cells and by transformed ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Sage H,Tupper J,Bramson R

    更新日期:1986-06-01 00:00:00

  • Timing of protooncogene expression varies in toxin-induced liver regeneration.

    abstract::Hepatic expression of the protooncogenes c-fos and c-myc occurs within 2 h after partial hepatectomy, and these immediate early genes are thought to prime the hepatocytes for subsequent proliferation. To examine whether such gene activation occurred in the setting of hepatocyte proliferation after toxic liver injury, ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Schmiedeberg P,Biempica L,Czaja MJ

    更新日期:1993-02-01 00:00:00

  • Functional discontinuities in prothymosin alpha caused by caspase cleavage in apoptotic cells.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Enkemann SA,Wang RH,Trumbore MW,Berger SL

    更新日期:2000-02-01 00:00:00

  • Exogenous nitric oxide elicits chemotaxis of neutrophils in vitro.

    abstract::Nitric oxide (NO) has been shown to be both an intercellular and intracellular messenger. We propose here that exogenous NO induces chemotactic locomotion of human neutrophils. Indeed, when human neutrophils were placed in a gradient of a nitric oxide donor (S-nitroso-N-acetylpenicillamine; SNAP), a directed locomotio...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Beauvais F,Michel L,Dubertret L

    更新日期:1995-12-01 00:00:00

  • Heat shock proteins within the mammalian cell cycle: relationship to thermal sensitivity, thermal tolerance, and cell cycle progression.

    abstract::We have measured endogenous and induced rates of 70-kD, 89-kD, and 110-kD heat shock proteins in highly pure G1-, S-, or G2-M phase fractions of Chinese hamster fibroblasts (CHO) separated by fluorescence-activated cell sorting (FACS). Relative rates of synthesis of all three polypeptides as measured by two-dimensiona...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


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  • Na+/H+ exchange is inactivated during mouse oocyte meiosis, facilitating glycine accumulation that maintains embryo cell volume.

    abstract::The coupled action of the Na(+)/H(+) exchanger NHE1 and the HCO3(-)/Cl(-) exchanger AE2 constitutes the principal mechanism for acute correction of decreased cell volume in mammalian somatic cells, while, when acting separately, they regulate intracellular pH. It was previously found that AE2 becomes inactivated durin...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zhou C,Fitzharris G,Alper SL,Baltz JM

    更新日期:2013-10-01 00:00:00

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    abstract::While retinoids have been demonstrated to inhibit growth of many tumor cells, including SCC cells, the molecular mechanism by which retinoids suppress growth has not been elucidated. We previously found that the growth of SCC cells was significantly inhibited by all-trans-retinoic acid (all-trans-RA) treatment, and th...

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    abstract::Vitamin C is an essential micronutrient for the development of male germ cells. In the gonad, the germ cells are isolated from the systemic circulation by the blood-testis barrier, which consists of a basal layer of Sertoli cells that communicate through an extensive array of tight junction complexes. To study the beh...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


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  • Biochemical genetic analysis of pyrimidine biosynthesis in mammalian cells. II. Isolation and characterization of a mutant of Chinese hamster ovary cells with defective dihydroorotate dehydrogenase (E.C. activity.

    abstract::A mutant (A204) of Chinese hamster ovary cells (CHO-K1), which is deficient in dihydroorotate (DHO) dehydrogenase (E.C. 1.3,3.1) activity, has been isolated by a replica plating procedure. The mutant does not show a requirement for exogenously added pyrimidines. Examination of intact cells shows that the mutant accumu...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


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  • Down-modulation of EGF receptors in cells transformed by the src oncogene.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


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    更新日期:1987-06-01 00:00:00

  • BMP-5 expression increases during chondrocyte differentiation in vivo and in vitro and promotes proliferation and cartilage matrix synthesis in primary chondrocyte cultures.

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    journal_title:Journal of cellular physiology

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  • Extinction of the human insulin gene expression in insulinoma x fibroblast somatic cell hybrids involves cis-acting DNA elements.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Besnard C,Monthioux E,Loràs P,Jami J,Daegelen D

    更新日期:1991-03-01 00:00:00

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    abstract::Conditioned medium from Sertoli cells, prepared from testes of 20-day-old rats, contains component(s) that inhibit the incorporation of [3H]-thymidine into DNA of peritubular myoid cells (PMC) and inhibit the proliferation of PMC. These components are trypsin-resistant, heat-stable compounds having a molecular weight ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Tung PS,Fritz IB

    更新日期:1991-06-01 00:00:00

  • Ceramide inhibits LPS-induced production of IL-5, IL-10, and IL-13 from mast cells.

    abstract::Mast cells are central regulators of allergic inflammation through production of various chemical mediators and cytokines. Bacterial infection occasionally worsens allergic inflammation. Although the exact mechanism of this phenomenon remains unclear, we have previously reported that LPS stimulates mast cells to produ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


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  • Loss of proline-rich tyrosine kinase 2 function induces spreading and motility of epithelial prostate cells.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


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    更新日期:2006-10-01 00:00:00

  • Phenotypic characterization of ouabain-resistant Aedes albopictus cells.

    abstract::The phenotype of a ouabain-resistant Aedes albopictus cell line has been partially characterized. Treatment of ouabain-sensitive cells with 0.005-1.0 mM ouabain resulted in an 80% reduction in the uptake of 86rubidium (86Rb+), an ion with an affinity for the K+ pump binding site; ouabain-resistant cells showed only a ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Mento SJ,Malinoski F,Stollar V

    更新日期:1979-12-01 00:00:00

  • Overexpression of the type-1 insulin-like growth factor receptor increases ligand-dependent proliferation and differentiation in bovine skeletal myogenic cultures.

    abstract::Previous studies demonstrated that overexpression of the type-1 insulin-like growth factor (IGF) receptor (IGF-1R) in skeletal myogenic cell lines increased proliferation and differentiation responses to IGF. However, it was unclear if such manipulations in primary, untransformed skeletal myogenic cells would result i...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Quinn LS,Haugk KL

    更新日期:1996-07-01 00:00:00

  • Inhibition of late endosome-lysosome fusion: studies on the mechanism by which isotonic-K+ buffers alter intracellular ligand movement.

    abstract::Incubation of alveolar macrophages or hepatocytes in media in which Na+ is replaced by K+ ("isotonic-K buffer") inhibited the movement of internalized ligand from late endosomes to lysosomes (Ward et al.: Journal of Cell Biology 110:1013-1022, 1990). In this study we investigate the mechanism responsible for the isoto...

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    pub_type: 杂志文章


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    更新日期:1990-12-01 00:00:00

  • Induction of plasminogen activator inhibitor 1 biosynthesis by hyperthermia.

    abstract::Hyperthermia is a clinical sign of inflammation and constitutes in itself an adaptive defense mechanism. The fibrinolytic system, a highly regulated proteolytic system, is involved in inflammatory processes. Plasminogen activator inhibitor 1 (PAI-1) is the principal inhibitor of the two activators of the fibrinolytic ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Bergonzelli GE,Kruithof EK

    更新日期:1991-08-01 00:00:00

  • The effects of training on hormonal concentrations in young soccer players.

    abstract::To test the hypothesis that football training would be accompanied by physiological adaptations and hormonal changes, we analyzed the effects of a whole football season on physical fitness and hormonal concentrations in youth football players. Male football players (n = 29, age 16.51 ± 0.7 years) in a regional profess...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Muscella A,Vetrugno C,Spedicato M,Stefàno E,Marsigliante S

    更新日期:2019-11-01 00:00:00

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    abstract::BA10-IR transformed cells, obtained by treating Syrian hamster embryo fibroblasts (HEF) with 7-methylbenz(a)anthracene and cultivated for a long period, are highly tumorigenic and grow in suspension as aggregates (spheroids) (Levy et al., 1976). They also grow in attached form or as spheroids in serum-free (S-) synthe...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


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    更新日期:1987-05-01 00:00:00

  • Effect of oxygen and endotoxin on lactate dehydrogenase release, 5-hydroxytryptamine uptake, and antioxidant enzyme activities in endothelial cells.

    abstract::We compared the effects of 95% O2 (hyperoxia) alone, endotoxin (20 ng/ml) alone, and 95% O2 plus endotoxin on the release of lactate dehydrogenase (LDH), uptake of 5-hydroxytryptamine (5-HT), and antioxidant enzyme activities in porcine pulmonary arterial and aortic endothelial cells in monolayer culture. Hyperoxia in...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Block ER,Patel JM,Sheridan NP

    更新日期:1985-02-01 00:00:00

  • Expression of P2 Purinergic Receptors in Mesenchymal Stem Cells and Their Roles in Extracellular Nucleotide Regulation of Cell Functions.

    abstract::Extracellular ATP and other nucleotides induce autocrine and/or paracrine purinergic signalling via activation of the P2 receptors on the cell surface, which represents one of the most common signalling mechanisms. Mesenchymal stem cells (MSC) are a type of multipotent adult stem cells that have many promising applica...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Jiang LH,Hao Y,Mousawi F,Peng H,Yang X

    更新日期:2017-02-01 00:00:00

  • C/EBPα and the Vitamin D Receptor Cooperate in the Regulation of Cathelicidin in Lung Epithelial Cells.

    abstract::1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) and the vitamin D receptor (VDR) have been reported to have an important role in the regulation of innate immunity. We earlier reported that the antimicrobial peptide cathelicidin is induced by 1,25(OH)2D3 in normal human bronchial epithelial cells with a resultant increase in an...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Dhawan P,Wei R,Sun C,Gombart AF,Koeffler HP,Diamond G,Christakos S

    更新日期:2015-02-01 00:00:00

  • PBK promotes aggressive phenotypes of cervical cancer through ERK/c-Myc signaling pathway.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ma H,Han F,Yan X,Qi G,Li Y,Li R,Yan S,Yuan C,Song K,Kong B

    更新日期:2020-11-13 00:00:00

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    abstract::The components of the insulin-like growth factor (IGF) axis and their roles in regulating proliferation and differentiation of the human colon adenocarcinoma cell line, Caco-2, have been investigated. Caco-2 cells proliferated in serum-free medium at 75% the rate observed in medium containing 10% fetal bovine serum. I...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


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    更新日期:1996-02-01 00:00:00

  • Role of diacylglycerol (DAG) in hormonal induction of S phase in hepatocytes: the DAG-dependent protein kinase C pathway is not activated by epidermal growth factor (EGF), but is involved in mediating the enhancement of responsiveness to EGF by vasopressi

    abstract::The role of diacylglycerol (DAG) in hormonal induction of S phase was investigated in primary cultures of rat hepatocytes. In this model, several agonists that bind to G protein-coupled receptors act as comitogens when added to the cells soon after plating (i.e., in Go/early Gl phase), while the cells are most respons...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


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    更新日期:1999-08-01 00:00:00

  • What role does pyroptosis play in microbial infection?

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Xia X,Wang X,Zheng Y,Jiang J,Hu J

    更新日期:2019-06-01 00:00:00

  • Effect of an alkaline shift on induction of the heat shock response in human fibroblasts.

    abstract::The simultaneous exposure of WI-38 human fibroblasts (HF) to a heat shock (45 degrees C, 30 min) and an alkaline shift (> or = pH 8.0) in the incubation medium increased and extended the expression of heat shock proteins (hsps). Hsp70 was the most prominent inducible hsp synthesized during the recovery phase after the...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Petronini PG,Alfieri R,Campanini C,Borghetti AF

    更新日期:1995-03-01 00:00:00