Abstract:
:Cementum, which shares common features with bone in terms of biochemical composition, is important for the homeostasis of periodontium during periodontitis and orthodontic treatment. Sirtuin 6 (SIRT6), as a member of the sirtuin family, plays key roles in the osteogenic differentiation of bone marrow mesenchymal stem cells. However, the involvement of SIRT6 in cementoblast differentiation and mineralization and the underlying mechanisms remain unknown. In this study, we observed that the expression of SIRT6 increased during cementoblast differentiation initially. Analysis of the gain- and loss-of-function indicated that overexpressing SIRT6 in OCCM-30 cells suppresses cementoblast differentiation and mineralization and downregulating SIRT6 promotes cementogenesis. GLUT1, a glucose transporter necessary in cementogenesis, was inhibited by SIRT6. Overexpressing GLUT1 in SIRT6-overexpressed OCCM-30 cells rescued the inhibitory effect of SIRT6 on cementoblast differentiation and mineralization. Moreover, AMPK was activated after overexpressing SIRT6 and inhibited cementoblast differentiation and mineralization. Downregulating the expression of SIRT6 inhibited AMPK activity. Meanwhile, GLUT1 overexpression significantly decreased AMPK activity. Overall, on one hand, SIRT6 inhibited cementoblast differentiation and mineralization by suppressing GLUT1. On the other hand, SIRT6 inhibited cementoblast differentiation and mineralization by activating the AMPK pathway. GLUT1 overexpression also rescued the increased AMPK pathway activated by SIRT6.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Huang L,Sun H,Song F,Cao Z,Jiang X,Zhang L,Li Z,Huang Cdoi
10.1002/jcp.27213subject
Has Abstractpub_date
2019-04-01 00:00:00pages
4005-4014issue
4eissn
0021-9541issn
1097-4652journal_volume
234pub_type
杂志文章abstract::The insulin-like growth factors (IGFs) are potent mitogens for malignant cell proliferation. The majority of secreted IGFs are bound to specific IGF-binding proteins (IGFBPs) that are secreted by a large number of cells. These proteins may either inhibit or enhance IGF actions. Breast carcinoma cells secrete a variety...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041580110
更新日期:1994-01-01 00:00:00
abstract::The involvement of Ras-GTPases in the development of renal fibrosis has been addressed in the last decade. We have previously shown that H- and N-Ras isoforms participate in the regulation of fibrosis. Herein, we assessed the role of K-Ras in cellular processes involved in the development of fibrosis: proliferation, m...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25340
更新日期:2016-10-01 00:00:00
abstract::Five-week-old female mice supplemented with estradiol and progesterone are able to respond to epidermal growth factor (EGF) and EGF-like growth factors (alpha-transforming growth factor [alpha-TGF] and crude mammary-derived growth factor) with local lobuloalveolar development when these growth factors are directly int...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041320324
更新日期:1987-09-01 00:00:00
abstract::Cell- and matrix-related parameters, which characterize the aging and differentiation process of cartilage in vivo, were measured in cultured chick epiphyseal chondrocytes during maintenance in a suspension culture for 34 days. A gradual decrease in the rates of proliferation and an increase in the size of the cells w...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041440204
更新日期:1990-08-01 00:00:00
abstract::We examined the interaction between OSU-03012 (also called AR-12) with phosphodiesterase 5 (PDE5) inhibitors to determine the role of the chaperone glucose-regulated protein (GRP78)/BiP/HSPA5 in the cellular response. Sildenafil (Viagra) interacted in a greater than additive fashion with OSU-03012 to kill stem-like GB...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24977
更新日期:2015-08-01 00:00:00
abstract::Nowadays, fat tissue transplantation is widely used in regenerative and reconstructive surgery. However, a shared method of lipoaspirate handling for ensuring a good quality fat transplant has not yet been established. The study was to identify a method to recover from the lipoaspirate samples the highest number of hu...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24965
更新日期:2015-08-01 00:00:00
abstract::Very little is known about the effects of pressure within the vascular system on EC phenotype. To study this, bovine aortic EC were seeded on rat type I collagen plates (2,000/cm2) and allowed to attach for 24 hours. The cells were exposed to either atmospheric, 40, 80, or 120 mm Hg pressure by placing them in a plexi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041580117
更新日期:1994-01-01 00:00:00
abstract::Males and females differ across a broad spectrum of morphological, physiological, and behavioral characters. In fact, sexually dimorphic traits typically contribute the largest component of phenotypic variance in most taxa that use sex to reproduce. However, we know very little about the mechanisms that maintain these...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.24979
更新日期:2015-10-01 00:00:00
abstract::Primary porcine bronchial epithelial cells (PBECs) are an ideal model to study the molecular and pathogenic mechanisms of various porcine respiratory pathogens. However, the short lifespan of primary PBECs greatly limit their application. Here, we isolated and cultured primary PBECs and established immortalized PBECs ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26942
更新日期:2018-12-01 00:00:00
abstract::Differentiation commitment events are essential for the initiation of hemopoiesis and, in one form or another, occur continuously during adult hemopoiesis. The most studied type of differentiation commitment decision a hemopoietic cell can make involves the alternative choice of self-renewal versus the formation of pr...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-4652(199711)173:2<131::AID-JCP9
更新日期:1997-11-01 00:00:00
abstract::Gastric cancer prognoses are persistently poor due to cancer's penchant to metastasize. As a crucial regulator of signal transducer and activator of transcription (STAT3) signaling, sirtuin 1's (SIRT1) function in gastric cancer has not been well understood. Here, we report upregulated expression of SIRT1 in tissues i...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28186
更新日期:2019-09-01 00:00:00
abstract::The expression of parathyroid hormone-related protein (PTHrP) was studied in a range of cell cultures representative of the osteoblast lineage and in rat calvarial sections. Primary newborn rat calvarial cells, a rat preosteoblastic cell line (UMR 201), a mouse stromal cell line (ST 2), a mouse calvaria-derived osteob...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199601)166:1<94::AID-JCP11
更新日期:1996-01-01 00:00:00
abstract::Butyrate and acetate are bacterial metabolites present in the large intestine lumen. Although butyrate is well known to inhibit the in vitro proliferation of human colon carcinoma cells in a process involving the hyperacetylation of specific nuclear histones, little is known about the possible link between butyrate me...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21556
更新日期:2009-01-01 00:00:00
abstract::We have recently described a novel nuclear antigen, AF-2, which is related to cell cycle dependent alterations of chromatin structure. We show by two parameter flow cytometry on a cell by cell basis that the antigen is accessible to specific monoclonal antibodies only in mitotic and postmitotic early G1-phase cells. T...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041490328
更新日期:1991-12-01 00:00:00
abstract::Accumulating evidence demonstrates that PKCι is an oncogene and prognostic marker that is frequently targeted for genetic alteration in many major forms of human cancer. Functional data demonstrate that PKCι is required for the transformed phenotype of lung, pancreatic, ovarian, prostate, colon, and brain cancer cells...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.22463
更新日期:2011-04-01 00:00:00
abstract::The main aim of this investigation was to determine whether a functional relationship existed between epidermal growth factor (EGF) and voltage-gated sodium channel (VGSC) upregulation, both associated with strongly metastatic prostate cancer cells. Incubation with EGF for 24 h more than doubled VGSC current density. ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21289
更新日期:2008-04-01 00:00:00
abstract::We have previously observed major differences in the phosphorylation of membrane proteins in sparse, proliferating versus confluent, quiescent pig aortic endothelial cells (EC) (Kazlauskas and DiCorleto, 1987). In the present study we examined whether EC growth state can influence the activity of a specific phosphoryl...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041360306
更新日期:1988-09-01 00:00:00
abstract::At 37 degrees C, undifferentiated murine teratocarcinoma cells (PCC4) are resistant to infection with SV40 and Polyoma virus. When infection is carried out at 31 degrees C, these cells become fully susceptible to a variety of polyoma virus strains, including wt, ts-a, and hr-t; they also display an increased susceptib...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041050104
更新日期:1980-10-01 00:00:00
abstract::During wound healing, myofibroblasts play a central role in matrix formation and wound contraction. At the end of healing, there is evidence that myofibroblasts disappear via apoptotic pathways. Hypertrophic scars are a fibroproliferative disorder that leads to considerable morbidity. It has been postulated that a def...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10415
更新日期:2004-03-01 00:00:00
abstract::In higher eukaryotes there is a link between time of replication and transcription. It is generally accepted that genes that are actively transcribed are replicated in the first half of S phase while inactive genes replicate in the second half of S phase. We have recently reported that in normal human fibroblasts ther...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.21156
更新日期:2007-11-01 00:00:00
abstract::Hepatocyte growth factor (HGF) plays a crucial role in the differentiation of skeletal muscle cells, where a process in which the retinoblastoma protein (pRb) has been implicated. We addressed the role of pRb in HGF-mediated effects on the proliferation and differentiation of adult skeletal muscle myoblasts. HGF shift...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10089
更新日期:2002-05-01 00:00:00
abstract::Interleukin (IL)-18 is a pro-inflammatory cytokine belonging to the IL-1 family, first identified for its interferon-γ-inducing properties. IL-18 regulates both T helper (Th) 1 and Th2 responses. It acts synergistically with IL-12 in the Th1 paradigm, whereas with IL-2 and without IL-12 it can induce Th2 cytokine prod...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.30008
更新日期:2021-03-01 00:00:00
abstract::Regulation of proliferation and differentiation in keratinocyte is a complex and dynamic process that involves activation of multiple signaling pathways triggered by different growth factors. Keratinocyte growth factor (KGF) is not only a potent mitogen, but differently from other growth factors, is a potent inducer o...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21056
更新日期:2007-09-01 00:00:00
abstract::Fetal brown adipocytes expressed uncoupling protein 1 (UCP1) mRNA, this expression being blunted throughout culture for 24 h in a serum-free medium. At physiological doses, either insulin-like growth factor I (IGF-I) or insulin turned out to be as potent as dibutyryl cAMP (dbcAMP) in increasing UCP1 gene transcription...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199807)176:1<99::AID-JCP12
更新日期:1998-07-01 00:00:00
abstract::Cover: The cover image, by Michaela R. Reagan et al., is based on the Perspective A Perspective on Malignancy in the Marrow, DOI 10.1002/jcp.25860. ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25563
更新日期:2017-12-01 00:00:00
abstract::The human chronic myeloid leukemia cell line K562 acquires several megakaryoblastoid features when cultured in the presence of the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA). We observed strongly increased secretion of several proteins into the culture media of K562 cells within a few hours of TPA trea...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041400115
更新日期:1989-07-01 00:00:00
abstract::Three-dimensional (3D) culture platforms have been explored to establish physiologically relevant cell culture environment and permit expansion scalability; however, little is known about the mechanisms underlying the regulation of pluripotency of human induced pluripotent stem cells (hiPSCs). This study elucidated ep...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.30211
更新日期:2020-12-10 00:00:00
abstract::Early biochemical changes associated with adjuvant stimulation of macrophage protein synthesis were studied using two murine macrophage cell lines, PU5-1.8 and J774.1. An induction of ornithine decarboxylase (ODC) was detected 2 hours after exposure of PU5-1.8 and J774.1 cells to two crude immunoadjuvants, BCG cell wa...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041200111
更新日期:1984-07-01 00:00:00
abstract::Breast cancer is a common cancer leading to many deaths among females. Cyclooxygenase-2 (COX-2) and interleukin-8 (IL-8) are two highly expressed inflammatory mediators to be induced by the protein kinase C (PKC) signaling via various inflammatory stimuli and both contribute significantly to cancer metastasis/progress...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24955
更新日期:2015-09-01 00:00:00
abstract::Although muscle satellite cells were identified almost 40 years ago, little is known about the induction of their proliferation and differentiation in response to physiological/pathological stimuli or to growth factors/cytokines. In order to investigate the role of the insulin-like growth factor (IGF)/IGF binding prot...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10227
更新日期:2003-04-01 00:00:00