Abstract：
:Dishevelled-associated activator of morphogenesis 1 (DAAM1) is a formin-family protein involved in nucleation of unbranched actin filaments and in cytoskeletal organization through Wnt-Dishevelled PCP pathway, which participates in essential biological processes, such as cell polarity, movement, and adhesion during morphogenesis and organogenesis. While its role has been investigated during development and in somatic cells, its potential association with the germinal compartment and reproduction is still unexplored. In this work, we assessed the possible association of DAAM1 with the morphogenesis of rat testis. We studied its expression and profiled its localization versus actin and tubulin, during the first wave of spermatogenesis and in the adult gonad (from 7 to 60 dpp). We show that, in mitotic phases, DAAM1 shares its localization with actin in Sertoli cells, gonocytes, and spermatogonia. Later, during meiosis, both proteins are found in spermatocytes, while only actin is detectable at the forming blood-testis barrier. DAAM1, then, follows the development of the acrosome system throughout spermiogenesis, and it is finally retained inside the cytoplasmic droplet in mature gametes, as corroborated by additional immunolocalization data on both rat and human sperm. Unlike the DAAM1, actin keeps its localization in Sertoli cells, and tubulin is associated with their protruding cytoplasm during the process. Our data support, for the first time, the hypothesis of a role for DAAM1 in cytoskeletal organization during Mammalian testis morphogenesis and gamete progression, while also hinting at its possible investigation as a morphological marker of germ cell and sperm physiology. J. Cell. Physiol. 231: 2172-2184, 2016. © 2016 Wiley Periodicals, Inc.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Pariante P,Dotolo R,Venditti M,Ferrara D,Donizetti A,Aniello F,Minucci Sdoi
10.1002/jcp.25330subject
Has Abstractpub_date
2016-10-01 00:00:00pages
2172-84issue
10eissn
0021-9541issn
1097-4652journal_volume
231pub_type
杂志文章abstract：:Oxidized-low density lipoprotein (Ox-LDL) has been shown to play an important role in impaired surfactant metabolism and transforming growth factor-β1 (TGF-β1) is a critical mediator in the pathogenesis of acute lung injury (ALI)/acute respiratory distress syndrome (ARDS). In this study, we investigated whether Ox-LDL...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.24005
更新日期：2012-09-01 00:00:00
abstract：:The ratio of mouse to rat albumin secreted by mouse hepatoma x rat hepatoma hybrid cells is constant (of the order of 5.0) irrespective of the total amounts produced. The present results establish for seven independent hybrid clones that the coordination in the ratio of mouse to rat product applies also at the level o...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041150211
更新日期：1983-05-01 00:00:00
abstract：:Uptake and phosphorylation of externally supplied [3H]-thymidine are fully stimulated in fertilized sea urchin eggs exposed to 5.0 micrograms/ml aphidicolin. As in untreated controls, the rate of uptake in aphidicolin-treated eggs increases greater than 50-fold shortly after fertilization, and greater than 85% of the ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041180107
更新日期：1984-01-01 00:00:00
abstract：:Human neutrophils labeled with chlortetracycline (CTC), commonly used as a probe of membrane-bound calcium, release lysosomal enzymes and exhibit a rapid decrease in fluorescence when exposed to the chemotactic peptide fMet-Leu-Phe or the lectin Con A. This decrease has been attributed to the release of calcium from a...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041170317
更新日期：1983-12-01 00:00:00
abstract：:Marrow stromal cells (MSCs) are stem-like cells having a striking somatic plasticity. In fact, besides differentiating into mesenchymal lineages (bone, cartilage, and fat), they are capable of differentiating into neurons and astrocytes in vitro and in vivo. The RB and RB2/P130 genes, belonging to the retinoblastoma g...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20026
更新日期：2004-08-01 00:00:00
abstract：:Four temperature-sensitive (ts) mutants of rat 3Y1 fibroblasts, representing independent complementation groups, cease to proliferate predominantly with a 2n DNA content, at the restrictive temperature (39.8 degrees C) (temperature arrest) or at the permissive temperature (33.8 degrees C) at a confluent cell density (...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041230310
更新日期：1985-06-01 00:00:00
abstract：:Breast cancer is a common cancer leading to many deaths among females. Cyclooxygenase-2 (COX-2) and interleukin-8 (IL-8) are two highly expressed inflammatory mediators to be induced by the protein kinase C (PKC) signaling via various inflammatory stimuli and both contribute significantly to cancer metastasis/progress...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.24955
更新日期：2015-09-01 00:00:00
abstract：:Glutathione S-transferases (GSTs) are enzymes that are involved in the detoxification of harmful electrophilic endogenous and exogenous compounds by conjugating with glutathione (GSH). The liver fluke GSTs have multifunctional roles in the host-parasite interaction, such as general detoxification and bile acid sequest...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27253
更新日期：2019-04-01 00:00:00
abstract：:We determined the mitogenic and comitogenic properties of tumor promoters in the indole alkaloid series; agents that differ structurally from 12-0-tetradecanoylphorbol-13-acetate (TPA), which is known to be a lymphocyte mitogen. Teleocidin and dihydroteleocidin B were mitogenic for human lymphocytes, and lyngbyatoxin ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041200106
更新日期：1984-07-01 00:00:00
abstract：:Long noncoding RNAs have been reported to be important regulators in numerous cancers. In this study, we found that HOXC13 antisense RNA (HOXC13-AS) was highly expressed in head and neck squamous carcinoma (HNSC) tissues in The Cancer Genome Atlas database. Nasopharyngeal carcinoma (NPC) belongs to HNSC. Therefore, we...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27915
更新日期：2019-08-01 00:00:00
abstract：:Mitochondrial transcription factor A (TFAM) is responsible for stability, maintenance, and transcriptional control of mitochondrial DNA (mtDNA). We have studied the expression and distribution of TFAM in the gametes and preimplantation embryos of the domestic pig (Sus scrofa). We hypothesized that TFAM is not present ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21528
更新日期：2008-11-01 00:00:00
abstract：:The cellular basis of the membrane-limited state of glucose utilization and the mechanism of the endogenous regulation of hexose uptake in dense monolayers of C6 glioma cells were investigated. In an earlier study, it was shown that at high rates of glucose transport and phosphorylation combined with the inhibition of...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041400105
更新日期：1989-07-01 00:00:00
abstract：:Although glucocorticoids strongly affect numerous biological processes including cell growth, development, and homeostasis, their effects on migration of human mesenchymal stem cells (hMSCs) are unclear. Therefore, we investigated the role of dexamethasone (DEX) and its related signaling pathways on migration of hMSCs...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.22383
更新日期：2011-03-01 00:00:00
abstract：:Exosomes contain regulatory signals such as lipids, proteins, and nucleic acids which can be transferred to adjacent or remote cells to mediate cell-to-cell communication. Exercise is a positive lifestyle for metabolic health and a nonpharmacological treatment of insulin resistance and metabolic diseases. Moreover, ex...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.28319
更新日期：2019-02-12 00:00:00
abstract：:Parkinsonism is one of the most common aging neurodegenerative disorders. This study aims to compare the therapeutic effect of stem cell versus its conditioned medium in the Parkinsonism model. Parkinsonism was induced by daily subcutaneous injection of 0.5 mg/kg of rotenone dissolved in dimethyl sulfoxide for 28 days...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29872
更新日期：2021-01-01 00:00:00
abstract：:Despite being densely packed with chromatin, nuclear bodies and a nucleoskeletal network, the nucleus is a remarkably dynamic organelle. Chromatin loops form and relax, RNA transcripts and transcription factors move diffusively, and nuclear bodies move. We show here that RNA splicing speckled domains (splicing speckle...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.25224
更新日期：2016-06-01 00:00:00
abstract：:The actions of prostaglandins (PG) on cAMP in dispersed chief cells from guinea pig stomach were examined and compared to the actions of these agents on pepsinogen secretion. Maximal concentrations of A, B, or E prostaglandins caused a 2-5-fold increase in pepsinogen secretion and cellular cAMP. The relative order of ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041320119
更新日期：1987-07-01 00:00:00
abstract：:Current osteoinductive protein therapy utilizes bolus administration of large doses of bone morphogenetic proteins (BMPs), which is costly, and may not replicate normal bone healing. The limited in vivo biologic activity of BMPs requires the investigation of growth factors that may enhance this activity. In this study...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.22639
更新日期：2011-11-01 00:00:00
abstract：:We have recently described a novel nuclear antigen, AF-2, which is related to cell cycle dependent alterations of chromatin structure. We show by two parameter flow cytometry on a cell by cell basis that the antigen is accessible to specific monoclonal antibodies only in mitotic and postmitotic early G1-phase cells. T...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041490328
更新日期：1991-12-01 00:00:00
abstract：:Bladder cancer (BCa) is one of the most prevalent cancers of the urinary system worldwide. Accumulating evidence suggests that long noncoding RNAs (lncRNAs) perform a vital function in the pathogenesis and progression of BCa. In the current study, we identified a novel lncRNA OXCT1-AS1 and investigated its role and po...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28037
更新日期：2019-08-01 00:00:00
abstract：:In order to promote cell attachment, fibronectin must first undergo activation by a suitable substrate. In this study, 52 materials have been surveyed for their ability a) to bind fibronectin, b) to activate the cell-adhesive property of fibronectin, and c) to support the growth of cells. Many plastics, polysaccharide...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041090314
更新日期：1981-12-01 00:00:00
abstract：:Using the Madin Darby canine kidney (MDCK) cell "calcium switch," we have previously demonstrated that, as MDCK cells establish contact and ultimately form tight junctions, there are marked global and localized changes in intracellular calcium at the sites of cell-cell contact (Nigam et al., 1992, Proc. Natl. Acad. Sc...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041590306
更新日期：1994-06-01 00:00:00
abstract：:The effects of ouabain on the growth of murine lymphoblasts in vitro have been studied. Exposure of cells to ouabain (0.1 mM) initially inhibited 86Rb+ uptake rate, reduced the intracellular potassium concentration, and decreased population growth rates. Continued exposure to the same ouabain concentration resulted in...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040850208
更新日期：1975-04-01 00:00:00
abstract：:We have previously isolated 3T3 cell variants unable to respond to specific mitogens. In this report we analyze the dominant and/or recessive nature of these variants. Two independently isolated EGF nonproliferative variants are unable to bind EGF. Hybrids between 3T3R5 cells (thymidine kinase deficient, ouabain-resis...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041230305
更新日期：1985-06-01 00:00:00
abstract：:It is known that down-regulation of cell surface platelet-derived growth factor (PDGF) receptors accompanies transformation by SV40. In this work human embryonic lung fibroblasts were used as a model system to study the effects of SV 40 on PDGF receptor expression. It is shown that transformation by SV 40 early region...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199601)166:1<12::AID-JCP2>
更新日期：1996-01-01 00:00:00
abstract：:The purpose of this study was to investigate the potential mechanism of interleukin-6 (IL-6) on the stimulation of excessive androgen secretion in human NCI-H295R adrenocortical cells. We performed transcriptome sequencing of cancer and paracancerous tissues obtained from functional adrenal cortical adenomas. The secr...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29748
更新日期：2020-12-01 00:00:00
abstract：:A new system for studying growth of normal human mammary epithelial cells in an in vivo environment using athymic nude mice is described. Human mammary epithelial cells dissociated from reduction mammoplasty specimens were embedded within collagen gels and subsequently transplanted subcutaneously into nude mice. Histo...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041630107
更新日期：1995-04-01 00:00:00
abstract：:The matricellular protein periostin is strongly expressed in collagen-rich connective tissues such as periodontal ligaments (PDLs), skeletal muscle, adipose tissue, tendons, skin, and bone. It is prominent in tumorigenesis, angiogenesis, and cardiac repair. It is localized in the periosteum and PDL, where it is seen i...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.24407
更新日期：2014-01-01 00:00:00
abstract：:Mitofusin 2 (MFN2) is a regulatory protein participating in mitochondria dynamics, cell proliferation, death, differentiation, and so on. This study aims at revealing the functional role of MFN2 in the pluripotency maintenance and primitive differetiation of embryonic stem cell (ESCs). A dox inducible silencing and ro...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29020
更新日期：2020-02-01 00:00:00
abstract：:To test the hypothesis that football training would be accompanied by physiological adaptations and hormonal changes, we analyzed the effects of a whole football season on physical fitness and hormonal concentrations in youth football players. Male football players (n = 29, age 16.51 ± 0.7 years) in a regional profess...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28673
更新日期：2019-11-01 00:00:00
