Properties and separation of T lymphocyte growth stimulatory activity (TL-GSA) and of granulocyte-macrophage colony stimulatory activity (GM-CSA) produced separately from two human T lymphocyte subpopulations.

Abstract:

:We have previously identified two stimulatory activities affecting blood cell maturation in PHA-stimulated human lymphocytes conditioned medium (PHA-LyCM). One was granulocyte-macrophage colony stimulatory activity (GM-CSA), and the other was T lymphocyte growth stimulatory activity (TL-GSA) in suspension culture. In this paper we have shown that although both activities can be produced from purified non-adherent human T lymphocytes, they are produced from two distinct subpopulations. The production of these activities was greatly enhanced by T cell mitogens. Both protein factors were relatively heat stable (56 degrees, 30 minutes), were sensitive to trypsin treatment and were specific for primate blood cells. These two activities were fractionated by means of ammonium sulfate precipitation, Sephadex G-150 gel filtration, DEAE cellulose and Con A-Sepharose column chromatographies. MW of the major peak estimated from the elution volume of gel filtration in the presence of 0.5 M NaCl was 40,000 for GM-CSA and 13,000 for TL-GSA. Results from Con A-Sepharose column showed that while about 70% of TL-GSA was bound to Con A, less than 25% of GM-CSA was bound. These observations show that the majority of TL-GSA and GM-CSA were separable by these two conventional column chromatographic methods.

journal_name

J Cell Physiol

authors

Wu AM

doi

10.1002/jcp.1041010206

subject

Has Abstract

pub_date

1979-11-01 00:00:00

pages

237-50

issue

2

eissn

0021-9541

issn

1097-4652

journal_volume

101

pub_type

杂志文章
  • Transglutaminase activity regulates differentiation, migration and fusion of osteoclasts via affecting actin dynamics.

    abstract::Osteoclasts, bone resorbing cells, derive from monocyte/macrophage cell lineage. Increased osteoclast activity is responsible for bone destruction in diseases such as osteoporosis, periodontitis and rheumatoid arthritis. Transglutaminases (TGs), protein crosslinking enzymes, were recently found involved in osteoclasto...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.26603

    authors: Sun H,Kaartinen MT

    更新日期:2018-09-01 00:00:00

  • High resolution analysis of cell cycle-correlated vimentin expression in asynchronously grown, TPA-treated MPC-11 cells by the novel flow cytometric multiparameter BrdU-Hoechst/PI and immunolabeling technique.

    abstract::High resolution, multiparameter analysis using the flow cytometric BrdU/Hoechst quenching technique has been applied to study cell cycle kinetics and vimentin expression in individual cells of asynchronously grown MPC-11 mouse plasmacytoma cell cultures treated with 12-O-tetradecanoylphorbol-13-acetate (TPA) to induce...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041610204

    authors: Giese G,Kubbies M,Traub P

    更新日期:1994-11-01 00:00:00

  • AMD3100 inhibits epithelial-mesenchymal transition, cell invasion, and metastasis in the liver and the lung through blocking the SDF-1α/CXCR4 signaling pathway in prostate cancer.

    abstract::Stromal cell-derived factor-1 (SDF-1) and CXC chemokine receptor 4 (CXCR4) have been found to be tightly correlated with the progression of prostate cancer (PC). In this study, we investigated the effects of an SDF-1α/CXCR4 inhibitor, AMD3100, on cell progression and metastasis potential of human PC cells. Human PC ce...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.27831

    authors: Zhu WB,Zhao ZF,Zhou X

    更新日期:2019-07-01 00:00:00

  • Decoupled syndecan 1 mRNA and protein expression is differentially regulated by angiotensin II in macrophages.

    abstract::It has been established that syndecan-1 is an important modulator of events relevant to acute tissue repair and chronic injury responses. The current studies were designed to examine syndecan-1 expression during atherosclerotic lesion formation and whether angiotensin II influences syndecan-1 expression in macrophages...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.21271

    authors: Wang W,Haller CA,Wen J,Wang P,Chaikof EL

    更新日期:2008-03-01 00:00:00

  • Differential extracellular matrix gene expression by fibroblasts during their proliferative life span in vitro and at senescence.

    abstract::Increasing evidence supports the idea that the finite proliferative life span of normal fibroblasts is a differentiation-like phenomenon. If this were correct, an ordered sequence of differential gene expression should be associated with the in vitro progression of cells from low passage to high passage (senescence). ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041510119

    authors: Choi AM,Olsen DR,Cook KG,Deamond SF,Uitto J,Bruce SA

    更新日期:1992-04-01 00:00:00

  • OSU-03012 and Viagra Treatment Inhibits the Activity of Multiple Chaperone Proteins and Disrupts the Blood-Brain Barrier: Implications for Anti-Cancer Therapies.

    abstract::We examined the interaction between OSU-03012 (also called AR-12) with phosphodiesterase 5 (PDE5) inhibitors to determine the role of the chaperone glucose-regulated protein (GRP78)/BiP/HSPA5 in the cellular response. Sildenafil (Viagra) interacted in a greater than additive fashion with OSU-03012 to kill stem-like GB...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.24977

    authors: Booth L,Roberts JL,Tavallai M,Nourbakhsh A,Chuckalovcak J,Carter J,Poklepovic A,Dent P

    更新日期:2015-08-01 00:00:00

  • Salidroside promotes rat spinal cord injury recovery by inhibiting inflammatory cytokine expression and NF-κB and MAPK signaling pathways.

    abstract::Spinal cord injury (SCI) is a public health problem in the world. The SCI usually triggers an excessive inflammatory response that brings about a secondary tissue wreck leading to further cellular and organ dysfunction. Hence, there is great potential of reducing inflammation for therapeutic strategies of SCI. In this...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.28124

    authors: Su Y,Zong S,Wei C,Song F,Feng H,Qin A,Lian Z,Fu F,Shao S,Fang F,Wu T,Xu J,Liu Q,Zhao J

    更新日期:2019-08-01 00:00:00

  • Acetylcholine Attenuates Hypoxia/Reoxygenation Injury by Inducing Mitophagy Through PINK1/Parkin Signal Pathway in H9c2 Cells.

    abstract::Acetylcholine (ACh) protected against cardiac injury via promoting autophagy and mitochondrial biogenesis, however, the involvement of mitophagy in ACh-elicited cardioprotection remains unknown. In the present study, H9c2 cardiomyocytes were subjected to hypoxia/reoxygenation (H/R) and ACh treatment during reoxygenati...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25215

    authors: Sun L,Zhao M,Yang Y,Xue RQ,Yu XJ,Liu JK,Zang WJ

    更新日期:2016-05-01 00:00:00

  • Control of alkaline phosphatase activity in C3H10T1/2 cells: role of retinoic acid and cell density.

    abstract::The enzyme alkaline phosphatase (AP) has been shown to be lost or inappropriately expressed during carcinogenesis in some tissues. Because retinoic acid (RA) appears to play a role in the normal regulation of the enzyme (RA up-regulates AP in a variety of cell types) we have suggested that altered AP expression in som...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041510204

    authors: Reese DH,Larsen RA,Hornicek FJ

    更新日期:1992-05-01 00:00:00

  • Homogentisic acid affects human osteoblastic functionality by oxidative stress and alteration of the Wnt/β-catenin signaling pathway.

    abstract::Alkaptonuria (AKU) is a rare disease correlated with deficiency of the enzyme homogentisate 1,2 dioxygenase, which causes homogentisic acid (HGA) accumulation. HGA is subjected to oxidation/polymerization reactions, leading to the production of a peculiar melanin-like pigmentation (ochronosis) after chronic inflammati...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.29575

    authors: Schiavone ML,Millucci L,Bernardini G,Giustarini D,Rossi R,Marzocchi B,Santucci A

    更新日期:2020-10-01 00:00:00

  • Analysis of the influences of the E5 transforming protein on kinetic parameters of epidermal growth factor binding and metabolism.

    abstract::The E5 protein of the bovine papillomavirus induces cellular transformation when transfected into NIH 3T3 cells, and the extent of focal transformation is enhanced by cotransfection with the epidermal growth factor (EGF) receptor (Martin et al., Cell 59:21-32, 1989). To determine whether E5 affects EGF:receptor intera...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041520206

    authors: Waters CM,Overholser KA,Sorkin A,Carpenter G

    更新日期:1992-08-01 00:00:00

  • Stem cells in lung repair and regeneration: Current applications and future promise.

    abstract::Lung diseases are major cause of morbidity and mortality worldwide. The progress in regenerative medicine and stem cell research in the lung are currently a fast-growing research topic that can provide solutions to these major health problems. Under normal conditions, the rate of cellular proliferation is relatively l...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.26414

    authors: Zhu Y,Chen X,Yang X,El-Hashash A

    更新日期:2018-10-01 00:00:00

  • Sertoli cells in culture secrete paracrine factor(s) that inhibit peritubular myoid cell proliferation: identification of heparinoids as likely candidates.

    abstract::Conditioned medium from Sertoli cells, prepared from testes of 20-day-old rats, contains component(s) that inhibit the incorporation of [3H]-thymidine into DNA of peritubular myoid cells (PMC) and inhibit the proliferation of PMC. These components are trypsin-resistant, heat-stable compounds having a molecular weight ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041470313

    authors: Tung PS,Fritz IB

    更新日期:1991-06-01 00:00:00

  • Gradients of natriuretic peptide precursor A (NPPA) in oviduct and of natriuretic peptide receptor 1 (NPR1) in spermatozoon are involved in mouse sperm chemotaxis and fertilization.

    abstract::Selective spermatozoa movement from storage of the oviduct to fertilization site is suggested to be a result of chemotaxis. In the present study, Natriuretic peptide precursor A (NPPA) induced sperm chemotaxis in capillaries and enhanced intracellular Ca(2+) level, both of which could be blocked by the Natriuretic Pep...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.22962

    authors: Bian F,Mao G,Guo M,Mao G,Wang J,Li J,Han Y,Chen X,Zhang M,Xia G

    更新日期:2012-05-01 00:00:00

  • Differential behavior of E-cadherin and occludin in their colocalization with ZO-1 during the establishment of epithelial cell polarity.

    abstract::At the initial stage of cell-cell contact of epithelial cells, primordial spot-like junctions are formed at the tips of thin cellular protrusions radiating from adjacent cells, where E-cadherin and ZO-1 are precisely coconcentrated (Yonemura et al., 1995, J. Cell Sci. 108:127-142). In fully polarized epithelial cells,...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199905)179:2<115::AID-JCP1

    authors: Ando-Akatsuka Y,Yonemura S,Itoh M,Furuse M,Tsukita S

    更新日期:1999-05-01 00:00:00

  • S100B causes apoptosis in a myoblast cell line in a RAGE-independent manner.

    abstract::S100B, a Ca(2+)-modulated protein with both intracellular and extracellular regulatory roles, is most abundant in astrocytes, is expressed in various amounts in several non-nervous cells and is also found in normal serum. Astrocytes secrete S100B, and extracellular S100B exerts trophic and toxic effects on neurons dep...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.10462

    authors: Sorci G,Riuzzi F,Agneletti AL,Marchetti C,Donato R

    更新日期:2004-05-01 00:00:00

  • Inhibition of late endosome-lysosome fusion: studies on the mechanism by which isotonic-K+ buffers alter intracellular ligand movement.

    abstract::Incubation of alveolar macrophages or hepatocytes in media in which Na+ is replaced by K+ ("isotonic-K buffer") inhibited the movement of internalized ligand from late endosomes to lysosomes (Ward et al.: Journal of Cell Biology 110:1013-1022, 1990). In this study we investigate the mechanism responsible for the isoto...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041450319

    authors: Ward DM,Hackenyos DP,Davis-Kaplan S,Kaplan J

    更新日期:1990-12-01 00:00:00

  • Effect of insoluble extracellular matrix molecules on Fas expression in epithelial cells.

    abstract::Fas, which functions to initiate a signal causing apoptosis, is expressed in epithelia, thus, suggesting a role in controlling cell number during states of cell and matrix turnover. In view of this, we hypothesized that cell-matrix interactions may be an important determinant of Fas expression in epithelial cells. To ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199803)174:3<285::AID-JCP2

    authors: Fine A,Miranda K,Farmer SR,Anderson NL

    更新日期:1998-03-01 00:00:00

  • Tuftsin-macrophage interaction: specific binding and augmentation of phagocytosis.

    abstract::The binding of [3H]tuftsin to normal and in vivo stimulated mouse peritoneal macrophage populations was studied at 22 degrees C. The [3H]tuftsin binding to thioglycollate-stimulated macrophages was shown to be rapid and saturable, with an equilibrium dissociation constant (K(D)) (calculated from a Scatchard plot) of 5...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041000106

    authors: Bar-Shavit Z,Stabinsky Y,Fridkin M,Goldman R

    更新日期:1979-07-01 00:00:00

  • LPS Induces Hyper-Permeability of Intestinal Epithelial Cells.

    abstract::Necrotizing Enterocolitis (NEC) is a severe inflammatory disorder leading to high morbidity and mortality rates. A growing body of evidence demonstrate the key role of the Toll like receptor 4 (TLR4) in NEC. This membranal receptor recognizes lipopolysaccharides (LPS) from the bacterial wall and triggers an inflammato...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25435

    authors: Bein A,Zilbershtein A,Golosovsky M,Davidov D,Schwartz B

    更新日期:2017-02-01 00:00:00

  • Membrane transport by guinea pig peritoneal exudate leukocytes: effect of phagocytosis on hexose and amino acid transport.

    abstract::Short term, carrier mediated transport of D-glucose, L-leucine and L-lysine by guinea pig peritoneal macrophages was characterized. Analysis of the amino acid transport demonstrated two-limbed double reciprocal plots suggesting two transport systems for each amino acid. The low concentration limb of the curves establi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1040930114

    authors: Straus DC,Imhoff JG,Bonventre PF

    更新日期:1977-10-01 00:00:00

  • Inhibition of PI 3-kinase and RAS blocks IGF-I and insulin-induced uncoupling protein 1 gene expression in brown adipocytes.

    abstract::Fetal brown adipocytes expressed uncoupling protein 1 (UCP1) mRNA, this expression being blunted throughout culture for 24 h in a serum-free medium. At physiological doses, either insulin-like growth factor I (IGF-I) or insulin turned out to be as potent as dibutyryl cAMP (dbcAMP) in increasing UCP1 gene transcription...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199807)176:1<99::AID-JCP12

    authors: Teruel T,Valverde AM,Navarro P,Benito M,Lorenzo M

    更新日期:1998-07-01 00:00:00

  • Regulatory role of microRNA-320a in the proliferation, migration, invasion, and apoptosis of trophoblasts and endothelial cells by targeting estrogen-related receptor γ.

    abstract::Recently, microRNAs (miRNAs) have been demonstrated to participate in many physiological and biological processes, especially by acting as circulating biomarkers or modulators in cell differentiation. Therefore, the aim of the current study was to clarify whether microRNA-320a (miR-320a) regulates the proliferation, m...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.26842

    authors: Liu RH,Meng Q,Shi YP,Xu HS

    更新日期:2018-01-01 00:00:00

  • Rspo1-LGR4 axis in BMSCs protects bone against radiation-induced injury through the mTOR-dependent autophagy pathway.

    abstract::While mesenchymal stem cells (MSCs) have been widely used to repair radiation-induced bone damage, the molecular mechanism underlying the effects of MSCs in the maintenance of bone homeostasis under radiation stress remains largely unknown. In this study, the role and mechanisms of R-spondin 1 (Rspo1)-leucine-rich rep...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.30051

    authors: Chen X,Chen L,Tan J,Zhang L,Xia J,Cheng B,Zhang W

    更新日期:2021-01-16 00:00:00

  • Restoration of microRNA-30b expression alleviates vascular calcification through the mTOR signaling pathway and autophagy.

    abstract::Pathological calcification represents an event that consequently leads to a distinct elevation in the morbidity and mortality of patients with chronic kidney disease (CKD) in addition to strengthening its correlation with hyperphosphatemia. Epigenomic regulation by specific microRNAs (miRNAs) is reported to be involve...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.28130

    authors: Xu TH,Qiu XB,Sheng ZT,Han YR,Wang J,Tian BY,Yao L

    更新日期:2019-08-01 00:00:00

  • Fluoro-Sorafenib (Regorafenib) effects on hepatoma cells: growth inhibition, quiescence, and recovery.

    abstract::To evaluate the growth-inhibitory properties of the potent multi-kinase antagonist Regorafenib (Fluoro-Sorafenib), which was synthesized as a more potent Sorafenib, a Raf inhibitor and to determine whether similar mechanisms were involved, human hepatoma cell lines were grown in the presence or absence of Regorafanib ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.24148

    authors: Carr BI,Cavallini A,Lippolis C,D'Alessandro R,Messa C,Refolo MG,Tafaro A

    更新日期:2013-02-01 00:00:00

  • Embryologic origin of endometriosis: analysis of 101 human female fetuses.

    abstract::The etiology of endometriosis, a gynecological disease characterized by the presence of endometrial glands and stroma outside the uterine cavity, is still unknown. Our research group has recently demonstrated the presence of ectopic endometrium in human female fetuses at different gestational ages. In this manuscript ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.22888

    authors: Signorile PG,Baldi F,Bussani R,Viceconte R,Bulzomi P,D'Armiento M,D'Avino A,Baldi A

    更新日期:2012-04-01 00:00:00

  • Light Sheet Microscopy to Measure Protein Dynamics.

    abstract::Visualizing protein dynamics is the key to a quantitative understanding of molecular mechanisms in biological systems. Recent developments in fluorescent microscopy techniques allow for novel experimental approaches to study stochastic localization, distribution, and movement of fluorescently labeled molecules in high...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25451

    authors: Rieckher M

    更新日期:2017-01-01 00:00:00

  • Coordinate secretion of rat and mouse albumin by mouse hepatoma x rat hepatoma hybrid cells directly reflects the intracellular concentration of the corresponding mRNAs.

    abstract::The ratio of mouse to rat albumin secreted by mouse hepatoma x rat hepatoma hybrid cells is constant (of the order of 5.0) irrespective of the total amounts produced. The present results establish for seven independent hybrid clones that the coordination in the ratio of mouse to rat product applies also at the level o...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041150211

    authors: Sellem CH,Cassio D,Sala-Trepat JM,Weiss MC

    更新日期:1983-05-01 00:00:00

  • Regulation of myelin basic protein gene transcription: identification of a distal cis-acting regulatory element.

    abstract::The myelin basic protein (MBP) gene contains sequences located upstream of its transcription start site which play a key role in glial-specific transcription of the MBP promoter. Earlier analysis of the 320 bp upstream regulatory sequence of MBP has revealed multiple cis-acting regulatory motifs which differentially r...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041630213

    authors: Haque NS,Haas S,Knobler RL,Khalili K

    更新日期:1995-05-01 00:00:00