Abstract:
:A method, based on the differing capacities of cells to adhere to a column of polyester fibres, has been described for separating human bone marrow cells into a nonadherent and an adherent fraction. The effect of this cell separation procedure on colony formation by erythroid progenitor cells was investigated. In contrast to the unseparated population, it was found that erythropoietin-dependent erythroid colony formation by nonadherent cells could be considerably enhanced by the addition of leukocyte conditioned medium to the cultures. Similar erythroid enhancing activity was also detected in a partially purified preparation of granulocytic colony stimulating activity obtained from human embryo kidney culture supernatants. Erythroid colony formation in the absence of added erythropoietin, by non-adherent bone marrow cells from patients with polycythemia rubra vera, were also enhanced by the addition of LCM to the cultures. This finding suggests that the enhancing factor in LCM may not be dependent on the presence of erythropoietin in the cultures for its activity. While the cellular mechanisms by which leukocyte conditioned medium enhances erythroid growth remain to be determined, the data presented provides strong evidence for the view that the plating efficiency of erythroid progenitor cells is determined not only be the concentration of erythropoietin, but also by the presence of leukocyte conditioned medium in the cultures.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Aye MTdoi
10.1002/jcp.1040910108subject
Has Abstractpub_date
1977-04-01 00:00:00pages
69-77issue
1eissn
0021-9541issn
1097-4652journal_volume
91pub_type
杂志文章abstract::Baculoviral IAP repeat containing 5 (BIRC5) gene encodes the important protein as survivin, a multifunctional protein, which is involved in cellular and molecular networks, progression of cell cycle, homeostasis, developmental morphogenesis, and apoptosis. The proximal BIRC5 promoter possesses specific binding sites f...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28725
更新日期:2019-11-01 00:00:00
abstract::Ankylosing spondylitis (AS) is a chronic immune-mediated inflammatory disease that affects both axial and peripheral skeletons as well as soft tissues. Recent investigations offer that disease pathogenesis is ascribed to a complex interplay of genetic, environmental, and immunological factors. Until now, there is no a...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.26500
更新日期:2018-08-01 00:00:00
abstract::Previous study suggested that the receptor component protein (RCP), one of the components of calcitonin gene-related peptide (CGRP) receptor, plays a multiple role in the cellular signal transduction. The study was designed to investigate whether or not the RCP involved in the regulation of caveolin-1/extracellular si...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26582
更新日期:2018-10-01 00:00:00
abstract::Non-small-cell lung cancer (NSCLC) remains the leading cause of cancer death worldwide. As a platinum-based chemotherapeutic drug, cisplatin has been used for over 30 years in NSCLC treatment while its effects are diminished by drug resistance. Therefore, we aimed to study the potential role of UCA1 in the development...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29266
更新日期:2020-04-01 00:00:00
abstract::Rat embryo fibroblasts were grown in medium containing 14C-leucine and 3H-thymidine. After a 24-hour chase in nonlabeled medium, cultures were placed in either fresh growth medium or medium containing 10-20 microgram/ml cycloheximide. Cell monolayers were processed at daily intervals for three days. Four hours prior t...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040970302
更新日期:1978-12-01 00:00:00
abstract::Serum-free mouse embryo cells, cultured in basal nutrient medium supplemented with insulin, transferrin, epidermal growth factor, fibronectin, and high-density lipoprotein, do not exhibit growth crisis, lack detectable chromosomal aberrations, are nontumorigenic in vivo, are dependent on epidermal growth factor for su...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041440110
更新日期:1990-07-01 00:00:00
abstract::Cell division, neurite formation and acetylcholinesterase activity were examined in a clone (NBA2) of mouse neuroblastoma cells maintained for up to 120 hours in medium with pH values between 6.6 and 8.0. Growth rate decreased as pH was reduced from 7.8 to 6.6. Generation time at pH 7.4 was 25 hours, while the rate of...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040910107
更新日期:1977-04-01 00:00:00
abstract::CRM197 is a naturally nontoxic diphtheria toxin mutant that binds and inhibits heparin-binding epidermal growth factor-like growth factor. CRM197 serves as carrier protein for vaccine and other therapeutic agents. CRM197 also inhibits the growth, migration, invasion, and induces apoptosis in various tumors. Vascular c...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24798
更新日期:2015-08-01 00:00:00
abstract::A neuroblastoma cell line was assessed for its capacity to bind tetanus toxin (TT) by using immunofluorescence and flow cytometry to analyze cells on a single cell basis. A clone of Neuro 2a, N2AB-1, was shown to bind variable amounts of TT per cell and this binding could be saturated by increasing doses of the toxin....
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041250317
更新日期:1985-12-01 00:00:00
abstract::Tissue expansion, a technique to enlarge the skin surface area with an expandable balloon, has been widely used in reconstructive surgery. Although the effect of tissue expansion on in vivo skin physiology and histology has been well documented, it remains unclear whether keratinocytes or other cell types are responsi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199710)173:1<64::AID-JCP8>
更新日期:1997-10-01 00:00:00
abstract::To test the hypothesis that football training would be accompanied by physiological adaptations and hormonal changes, we analyzed the effects of a whole football season on physical fitness and hormonal concentrations in youth football players. Male football players (n = 29, age 16.51 ± 0.7 years) in a regional profess...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28673
更新日期:2019-11-01 00:00:00
abstract::Osteoclasts mediate bone resorption by secretion at the site of bone attachment. This process depends on calmodulin concentrated at a specialized acid-secreting membrane. We hypothesized that increased calmodulin and bone attachment were required for acid secretion. We tested this by studying calmodulin, bone attachme...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199612)169:3<411::AID-JCP1
更新日期:1996-12-01 00:00:00
abstract::Aberrations of DNA methylation are early events in the development of tumors. In this study, we investigated the DNA methylation status of growth hormone secretagogue receptor (GHSR), a promising pan-cancer biomarker, in gastric cancer (GC). Initially, data sets from DNA methylation and gene expression studies availab...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28179
更新日期:2019-01-24 00:00:00
abstract::Electrophysiologic properties of cultured human keratinocytes were studied using the patch voltage-clamp technique. Undifferentiated, proliferative keratinocytes grown in low Ca2+ medium had an average resting membrane potential of -24 mV. Voltage-clamp experiments showed that these cells had two membrane ionic curren...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041430103
更新日期:1990-04-01 00:00:00
abstract::Small hepatocytes (SHs) are a subpopulation of hepatocytes that have high growth potential in culture and can differentiate into mature hepatocytes (MHs). The activin (Act)/follistatin (Fst) system critically contributes to homeostasis of cell growth in the normal liver. ActA and ActB consist of two disulfide-linked I...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22971
更新日期:2012-06-01 00:00:00
abstract::Mammalian sperm require to spend a limited period of time in the female reproductive tract to become competent to fertilize in a process called capacitation. It is well established that HCO3- is essential for capacitation because it activates the atypical soluble adenylate cyclase ADCY10 leading to cAMP production, an...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25634
更新日期:2017-06-01 00:00:00
abstract::The increasing rate of mortality and morbidity because of cardiac diseases has called for efficient therapeutic needs. With the advancement in cell-based therapies, stem cells are abundantly studied in this area. Nearly, all sources of stem cells are experimented to treat cardiac injuries. Tissue engineering has also ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.28330
更新日期:2019-02-27 00:00:00
abstract::Progesterone is an endogenous immunomodulator and can suppress T-cell activation during pregnancy. We have previously shown that the non-genomic effects of progesterone, especially acidification, are exerted via plasma membrane sites and suppress cellular genomic responses to mitogens. This study aimed to show that ac...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20962
更新日期:2007-05-01 00:00:00
abstract::In culture, B16/C3 murine melanoma cells grown in the presence of serum undergo melanogenesis at a specific time after plating. At this time, melanin is synthesized intracellularly and then secreted into the extracellular culture fluid. We have found that melanin secretion is dependent on the presence of serum in the ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041130318
更新日期:1982-12-01 00:00:00
abstract::Nowadays, fat tissue transplantation is widely used in regenerative and reconstructive surgery. However, a shared method of lipoaspirate handling for ensuring a good quality fat transplant has not yet been established. The study was to identify a method to recover from the lipoaspirate samples the highest number of hu...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24965
更新日期:2015-08-01 00:00:00
abstract::Cardiac hypertrophy (CH), characterized by the enlargement of cardiomyocytes, fibrosis and apoptosis, is one of the leading causes of death worldwide. Despite the advances in cardiovascular research, there remains a need to further investigate the signaling pathways that mediate CH in order to identify novel therapeut...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.25884
更新日期:2017-12-01 00:00:00
abstract::NGF appears to be involved in spermatogenesis. However, mice lacking NGF or TrkA genes do not survive more than a few days whereas p75(NTR) knockout mice are viable and fertile. Therefore, we addressed the effect of betaNGF on spermatogenesis by using the systems of rat germ cell culture we established previously. bet...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20805
更新日期:2007-01-01 00:00:00
abstract::The 10th annual meeting of the Italian Society for Virology (SIV) comprised seven plenary sessions focused on: General virology and viral genetics; Virus-Host interaction and pathogenesis; Viral oncology; Emerging viruses and zoonotic, foodborne and environmental pathways of transmission; Viral immunology and vaccines...
journal_title:Journal of cellular physiology
pub_type: 临床试验,杂志文章
doi:10.1002/jcp.24055
更新日期:2012-07-01 00:00:00
abstract::Neurodegenerative diseases, including Alzheimer's disease (AD), Parkinson's disease (PD), and Huntington's disease (HD), are incurable diseases characterized by progressive loss of cognitive or motor function, which construct a serious threat to the life quality of aging populations and their life spans. Apelin is an ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.29001
更新日期:2020-02-01 00:00:00
abstract::Several papers report a hypoxia-induced upregulation of the endothelial nitric oxide synthase (eNOS) mRNA expression. Since there is no known hypoxia-sensitive element binding site in the eNOS promoter, we reasoned that the effect of hypoxia could be simulated by a metabolically elicited alteration of the redox state....
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1092
更新日期:2001-07-01 00:00:00
abstract::Pain can vary over the estrous cycle as a result of changes in estradiol concentration but the mechanism causing this variation is unclear. Because the thalamus is important in pain control, gene expression in the lateral thalamus (ventral posteromedial, ventral posterolateral, reticular thalamic nuclei) was screened ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25198
更新日期:2016-05-01 00:00:00
abstract::Invasion of tumor cells is the primary cause of therapeutic failure in the treatment of malignant chondrosarcomas. Glial cell-derived neurotrophic factor (GDNF) plays a crucial role in migration and metastasis of human cancer cells. Integrins are the major adhesive molecules in mammalian cells. Here we found that GDNF...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21802
更新日期:2009-08-01 00:00:00
abstract::Interleukin (IL)-8 is released both in visceral adipose tissue and in contracting skeletal muscles. In this study, we examined cellular pathways associated with muscle hypertrophy, chosen on the basis of microRNA profiling, in differentiating rat primary skeletal muscle cells (RSkMC) treated with IL-8 (1 ng/ml) for 11...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28568
更新日期:2019-11-01 00:00:00
abstract:BACKGROUND:Surgical stress has been suggested to facilitate colon cancer growth and metastasis. However, the precise mechanisms by which surgical trauma promotes colon cancer progression remain poorly understood. METHODS:To unravel the mechanisms underlying surgery-induced colon cancer progression, a syngenic transpla...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27245
更新日期:2019-04-01 00:00:00
abstract::Stromal cell-derived factor-1 (SDF-1) and CXC chemokine receptor 4 (CXCR4) have been found to be tightly correlated with the progression of prostate cancer (PC). In this study, we investigated the effects of an SDF-1α/CXCR4 inhibitor, AMD3100, on cell progression and metastasis potential of human PC cells. Human PC ce...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27831
更新日期:2019-07-01 00:00:00