Involvement of CGRP-RCP in the caveolin-1/ERK1/2 signal pathway in the static pressure-induced proliferation of vascular smooth muscle cells.


:Previous study suggested that the receptor component protein (RCP), one of the components of calcitonin gene-related peptide (CGRP) receptor, plays a multiple role in the cellular signal transduction. The study was designed to investigate whether or not the RCP involved in the regulation of caveolin-1/extracellular signal-regulated kinases-1 and -2 (ERK1/2) signal pathway in the vascular smooth muscle cells (VSMCs) proliferation induced by static pressure. Mouse-derived VSMCs line A10 (A10 VSMCs) was served as project in this experiment. Results showed that the A10 VSMCs viability and proliferating cell nuclear antigen (PCNA) expression which were increased by static pressure were inhibited by pretreatment of CGRP. In like manner, the expressions of the decreased-caveolin-1 and the increased-phosphorylated ERK1/2 (p-ERK1/2) induced by static pressure were significantly reversed by pretreatment of CGRP, respectively. Meanwhile, the expression of RCP was up-regulated by the static pressure. Silence of RCP gene with the small interrupt RNA (siRNA) not only significantly increased A10 VSMC proliferation but also increased the expression of p-ERK1/2 in response to static pressure. When treatment of A10 VSMCs with 120-mmHg static pressure for different time, however, the protein band of caveolin-1 and RCP was the least at time point of 10 min, but the p-ERK1/2 expression was the most maximum. In conclusion, RCP maybe involved in the static pressure-induced A10 VSMCs proliferation by regulation of caveolin-1/ERK1/2 signal pathway.


J Cell Physiol


Guo F,Yang L,Luo J,Quan H,Wang Z,Peng H,Hong C,Li J,Jiang Z,Zhang L,Qin X




Has Abstract


2018-10-01 00:00:00












  • Curcumin, hemostasis, thrombosis, and coagulation.

    abstract::Atherothrombotic cardiovascular disease is a major cause of mortality throughout the world. Platelet activation and aggregation play a central role in hemostasis and thrombosis. Herbal medicines have been traditionally used in the management of cardiovascular disease and can help in modifying its progression, particul...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Keihanian F,Saeidinia A,Bagheri RK,Johnston TP,Sahebkar A

    更新日期:2018-06-01 00:00:00

  • Dexamethasone interferes with osteoblasts formation during osteogenesis through altering IGF-1-mediated angiogenesis.

    abstract::Dexamethasone (Dex), a synthetic glucocorticoid (GC) with long-lasting treatment effects, has been proved to exert a modulatory effect on osteoblast proliferation and differentiation during embryonic osteogenesis. However, it is still controversial if Dex exposure influences endochondral ossification and the underlyin...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Xu S,Guo R,Li PZ,Li K,Yan Y,Chen J,Wang G,Brand-Saberi B,Yang X,Cheng X

    更新日期:2019-01-22 00:00:00

  • In vitro evaluation of different methods of handling human liposuction aspirate and their effect on adipocytes and adipose derived stem cells.

    abstract::Nowadays, fat tissue transplantation is widely used in regenerative and reconstructive surgery. However, a shared method of lipoaspirate handling for ensuring a good quality fat transplant has not yet been established. The study was to identify a method to recover from the lipoaspirate samples the highest number of hu...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Palumbo P,Miconi G,Cinque B,La Torre C,Lombardi F,Zoccali G,Orsini G,Leocata P,Giuliani M,Cifone MG

    更新日期:2015-08-01 00:00:00

  • The calcineurin-nuclear factor of activated T cells signaling pathway mediates the effect of corticotropin releasing factor and urocortins on catecholamine synthesis.

    abstract::The biological effects of the Corticotropin-releasing factor (CRF) family of neuropeptides are mediated by mobilization of [Ca(2+)]. Aim of the current work was to examine if the calcineurin/NFAT (nuclear factor of activated T-cells) signaling pathway is involved in the effect of CRF peptides in catecholamine synthesi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Dermitzaki E,Tsatsanis C,Gravanis A,Margioris AN

    更新日期:2012-05-01 00:00:00

  • A fractionation procedure of mouse bone marrow cells yielding exclusively pluripotent stem cells and committed progenitors.

    abstract::The cell surface phenotype of pluripotent hemopoietic stem cells (CFU-S) and committed progenitors (CFU-C1, CFU-C2, BFU-E) of mouse bone marrow was analyzed with respect to their binding of wheat germ agglutinin (WGA) and two monoclonal antibodies, anti-GM-1.2 and anti-PGP-1. Stained cells were fractionated on the bas...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Bauman JG,Wagemaker G,Visser JW

    更新日期:1986-07-01 00:00:00

  • Rapamycin attenuates BAFF-extended proliferation and survival via disruption of mTORC1/2 signaling in normal and neoplastic B-lymphoid cells.

    abstract::B cell activating factor from the TNF family (BAFF) stimulates B-cell proliferation and survival, but excessive BAFF promotes the development of aggressive B cells leading to malignant and autoimmune diseases. Recently, we have reported that rapamycin, a macrocyclic lactone, attenuates human soluble BAFF (hsBAFF)-stim...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zeng Q,Qin S,Zhang H,Liu B,Qin J,Wang X,Zhang R,Liu C,Dong X,Zhang S,Huang S,Chen L

    更新日期:2018-01-01 00:00:00

  • Diminished calcium influx in lectin-stimulated T cells from old mice.

    abstract::T lymphocytes from aged donors function poorly, but the biochemical basis for the defect remains uncertain. We tested the hypothesis that T cells from old mice had a diminished ability to transmit extracellular signals into the cytoplasm, by measuring intracellular free calcium concentrations (Cai) in T cells stimulat...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Miller RA,Jacobson B,Weil G,Simons ER

    更新日期:1987-08-01 00:00:00

  • Effects of IGFBP3 gene silencing mediated inhibition of ERK/MAPK signaling pathway on proliferation, apoptosis, autophagy, and cell senescence in rats nucleus pulposus cells.

    abstract:OBJECTIVE:Disc degeneration is the common life-threatening disease characterized by flank pain. The gene expression of insulin-like growth factor binding protein 3 (IGFBP3) is increased in patients with disc degeneration, however, its mechanism is still unknown. This study aimed to investigate the influence of IGFBP3 g...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Chen G,Zhou X,Xu Z

    更新日期:2019-06-01 00:00:00

  • P2Y2 receptor promotes intestinal microtubule stabilization and mucosal re-epithelization in experimental colitis.

    abstract::P2Y(2) receptor expression is increased in intestinal epithelial cells (IECs) during inflammatory bowel diseases (IBDs). In this context, P2Y(2) stimulates PGE(2) release by IECs, suggesting a role in wound healing. For this study, we have used the non-cancerous IEC-6 cell line. IEC-6 cell migration was determined usi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Degagné E,Degrandmaison J,Grbic DM,Vinette V,Arguin G,Gendron FP

    更新日期:2013-01-01 00:00:00

  • Altering microtubule stability affects microtubule clearance and nuclear extrusion during erythropoiesis.

    abstract::Mammalian erythrocytes are highly specialized cells that have adapted to lose their nuclei and cellular components during maturation to ensure oxygen delivery. Nuclear extrusion, the most critical event during erythropoiesis, represents an extreme case of asymmetric partitioning that requires a dramatic reorganization...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Xie S,Yan B,Feng J,Wu Y,He N,Sun L,Zhou J,Li D,Liu M

    更新日期:2019-11-01 00:00:00

  • Porphyromonas gingivalis lipopolysaccharide induces shedding of syndecan-1 expressed by gingival epithelial cells.

    abstract::Syndecans are constitutively shed from growing epithelial cells as the part of normal cell surface turnover. However, increased serum levels of the soluble syndecan ectodomain have been reported to occur during bacterial infections. The aim of this study was to evaluate the potential of lipopolysaccharide (LPS) from t...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Andrian E,Grenier D,Rouabhia M

    更新日期:2005-07-01 00:00:00

  • Downregulation of uPAR promotes urokinase translocation into the nucleus and epithelial to mesenchymal transition in neuroblastoma.

    abstract::The urokinase system is involved in a variety of physiological processes, such as fibrinolysis, matrix remodeling, wound healing, and regeneration. Upon binding to its cognate receptor urokinase-type plasminogen activator receptor (uPAR), urokinase-type plasminogen activator (uPA) catalyzes the conversion of plasminog...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Semina EV,Rubina KA,Shmakova AA,Rysenkova KD,Klimovich PS,Aleksanrushkina NA,Sysoeva VY,Karagyaur MN,Tkachuk VA

    更新日期:2020-09-01 00:00:00

  • Cucurbitacin B suppresses proliferation of pancreatic cancer cells by ceRNA: Effect of miR-146b-5p and lncRNA-AFAP1-AS1.

    abstract::Cucurbitacin B (CuB) is a natural tetracyclic triterpene product that displays antitumor activity against a wide variety of cancers. In this study, we explored the antipancreatic cancer activity of CuB via the inhibition of expression of the cancer-related long noncoding RNA, actin filament-associated protein 1-antise...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zhou J,Liu M,Chen Y,Xu S,Guo Y,Zhao L

    更新日期:2019-04-01 00:00:00

  • Over-expression of DEC1 inhibits myogenic differentiation by modulating MyoG activity in bovine satellite cell.

    abstract::Differentiated embryo chondrocyte 1 (DEC1), a member of basic-helix-loop-helix transcription factor Bhlhe40, also called stimulated by retinoic acid 13, STRA13, plays an important role in the regulation of adipogenesis, tumorigenesis, peripheral circadian output, response to hypoxia, and development of metabolic syndr...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Huang Y,Lai X,Hu L,Lei C,Lan X,Zhang C,Ma Y,Zheng L,Bai YY,Lin F,Chen H

    更新日期:2018-12-01 00:00:00

  • MALAT1 via microRNA-17 regulation of insulin transcription is involved in the dysfunction of pancreatic β-cells induced by cigarette smoke extract.

    abstract::Cigarettes contain various chemicals with the potential to influence metabolic health. Exposure to cigarette smoke causes a dysfunction in pancreatic β-cells and impairs insulin production. However, the mechanisms for cigarette smoke-induced reduction of insulin remain largely unclear. Data from 558 patients with diab...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Sun Q,Xu H,Xue J,Yang Q,Chen C,Yang P,Han A,Tu Q,Lu J,Gao X,Xiang Q,Liu Q

    更新日期:2018-11-01 00:00:00

  • Human mesenchymal stromal cells in adhesion to cell-derived extracellular matrix and titanium: Comparative kinome profile analysis.

    abstract::The extracellular matrix (ECM) physically supports cells and influences stem cell behaviour, modulating kinase-mediated signalling cascades. Cell-derived ECMs have emerged in bone regeneration as they reproduce physiological tissue-architecture and ameliorate mesenchymal stromal cell (MSC) properties. Titanium scaffol...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Baroncelli M,Fuhler GM,van de Peppel J,Zambuzzi WF,van Leeuwen JP,van der Eerden BCJ,Peppelenbosch MP

    更新日期:2019-03-01 00:00:00

  • Comprehensive landscape of STEAP family functions and prognostic prediction value in glioblastoma.

    abstract::Glioblastoma (GBM) is the most common, malignant, and deadly primary glioma. Six-transmembrane epithelial antigen of prostate (STEAP) family is involved in tumorigenesis; here, we have explored the biological function and the prognostic value of the STEAP family in GBM. Differentially expressed STEAP genes in tumor an...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Chen H,Xu C,Yu Q,Zhong C,Peng Y,Chen J,Chen G

    更新日期:2020-09-23 00:00:00

  • Comparative genomics of NAD(P) biosynthesis and novel antibiotic drug targets.

    abstract::NAD(P) is an indispensable cofactor for all organisms and its biosynthetic pathways are proposed as promising novel antibiotics targets against pathogens such as Mycobacterium tuberculosis. Six NAD(P) biosynthetic pathways were reconstructed by comparative genomics: de novo pathway (Asp), de novo pathway (Try), NmR pa...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Bi J,Wang H,Xie J

    更新日期:2011-02-01 00:00:00

  • Recombinant non-collagenous domain of alpha2(IV) collagen causes involution of choroidal neovascularization by inducing apoptosis.

    abstract::Vascular endothelial cells receive proangiogenic or antiangiogenic signals from components of extracellular matrix (ECM) depending upon the situation and many molecular signals can have opposite effects in different vascular beds. Tissue inhibitor of metalloproteinase 1 is antiangiogenic in several tissues, but promot...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Lima E Silva R,Kachi S,Akiyama H,Shen J,Aslam S,Yuan Gong Y,Khu NH,Hatara MC,Boutaud A,Peterson R,Campochiaro PA

    更新日期:2006-07-01 00:00:00

  • Increased epidermal growth factor receptor in an estrogen-responsive, adriamycin-resistant MCF-7 cell line.

    abstract::We examined the expression of the estrogen and epidermal growth factor (EGF) receptors in a drug-resistant subline of MCF-7 cells in order to study potential alterations in hormone dependence or in the growth factor pathway that could be related to the development of drug resistance in human breast cancer. The drug-re...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Dickstein B,Valverius EM,Wosikowski K,Saceda M,Pearson JW,Martin MB,Bates SE

    更新日期:1993-10-01 00:00:00

  • Stimulation of LDL receptor activity in Hep-G2 cells by a serum factor(s).

    abstract::The regulation of low-density lipoprotein (LDL) receptor activity in the human hepatoma cell line Hep-G2 by serum components was examined. Incubation of dense monolayers of Hep-G2 cells with fresh medium containing 10% fetal calf serum (FM) produced a time-dependent increase in LDL receptor activity. Uptake and degrad...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ellsworth JL,Brown C,Cooper AD

    更新日期:1988-05-01 00:00:00

  • Isolation, Characterization, and Establishment of Spontaneously Immortalized Cell Line HRPE-2S With Stem Cell Properties.

    abstract::The retinal pigment epithelium is a monolayer of highly specialized pigmented cells located between the neural retina and the Bruch's membrane of the choroid. RPE cells play a crucial role in the maintenance and function of the underlying photoreceptors. This study introduces a spontaneously arising human retinal pigm...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Shams Najafabadi H,Soheili ZS,Samiei S,Ahmadieh H,Ranaei Pirmardan E,Masoumi M

    更新日期:2017-10-01 00:00:00

  • Characterizing the multiple roles of FGF-2 in SOD1G93A ALS mice in vivo and in vitro.

    abstract::We have previously shown that knockout of fibroblast growth factor-2 (FGF-2) and potential compensatory effects of other growth factors result in amelioration of disease symptoms in a transgenic mouse model of amyotrophic lateral sclerosis (ALS). ALS is a rapidly progressive neurological disorder leading to degenerati...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Kefalakes E,Böselt S,Sarikidi A,Ettcheto M,Bursch F,Naujock M,Stanslowsky N,Schmuck M,Barenys M,Wegner F,Grothe C,Petri S

    更新日期:2019-05-01 00:00:00

  • Nestin expression is dynamically regulated in cardiomyocytes during embryogenesis.

    abstract::The transcriptional factors implicated in the expression of the intermediate filament protein nestin in cardiomyocytes during embryogenesis remain undefined. In the heart of 9,5-10,5 day embryonic mice, nestin staining was detected in atrial and ventricular cardiomyocytes and a subpopulation co-expressed Tbx5. At late...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Hertig V,Matos-Nieves A,Garg V,Villeneuve L,Mamarbachi M,Caland L,Calderone A

    更新日期:2018-04-01 00:00:00

  • Induction of angiogenesis by smooth muscle cell-derived factor: possible role in neovascularization in atherosclerotic plaque.

    abstract::The development of atherosclerotic plaque is associated with neovascularization in the thickened intima and media of vascular walls. Neovascularization may have a role in the progression of atherosclerotic plaque as well as in the development of intraplaque hemorrhage. However, the mechanism and stimulus for neovascul...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Kuzuya M,Satake S,Esaki T,Yamada K,Hayashi T,Naito M,Asai K,Iguchi A

    更新日期:1995-09-01 00:00:00

  • Temporal studies on the tissue compartmentalization of bone sialoprotein (BSP), osteopontin (OPN), and SPARC protein during bone formation in vitro.

    abstract::To study the role of noncollagenous proteins in bone formation, the synthesis and tissue distribution of BSP (bone sialoprotein), OPN (osteopontin) and SPARC (secreted protein acidic and rich in cysteine) were analyzed using pulse-chase and continuous labeling protocols during bone formation by cultures of rat calvari...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Kasugai S,Nagata T,Sodek J

    更新日期:1992-09-01 00:00:00

  • Combined treatment with systemic resveratrol and resveratrol preconditioned mesenchymal stem cells, maximizes antifibrotic action in diabetic cardiomyopathy.

    abstract::Wnt/β-catenin signaling pathway plays a crucial role in diabetic cardiomyopathy (DCM), thus we aimed at investigating the effect of one therapeutic approach with resveratrol (RSV) given systemically and combined treatment of RSV with mesenchymal stem cells (MSCs) that was either RSV-preconditioned or not on Wnt/β-cate...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: ShamsEldeen AM,Ashour H,Shoukry HS,Fadel M,Kamar SS,Aabdelbaset M,Rashed LA,Ammar HI

    更新日期:2019-07-01 00:00:00

  • Biosensors for real-time monitoring of physiological processes in the musculoskeletal system: A systematic review.

    abstract::Biosensors are composed of (bio)receptors, transducers, and detection systems and are able to convert the biological stimulus into a measurable signal. This systematic review evaluates the current state of the art of innovation and research in this field, identifying the biosensors that in vitro monitor the musculoske...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Veronesi F,Tschon M,Visani A,Fini M

    更新日期:2019-12-01 00:00:00

  • The origin of chicken hematopoietic colonies as assayed in semisolid agar.

    abstract::This investigation was undertaken to determine whether primitive stem cells and/or fully differentiated macrophages were the source of in vitro colonies derived from hematopoietic tissues. The chicken colony-forming cell (CFC) present in uncultured yolk sac was a nonadherent, presumably undifferentiated cell. The effi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Dodge WH,Silva RF,Moscovici C

    更新日期:1975-02-01 00:00:00

  • Acute exercise reduces insulin resistance-induced TRB3 expression and amelioration of the hepatic production of glucose in the liver of diabetic mice.

    abstract::TRB3 (a mammalian homolog of Drosophila) is emerging as an important player in the regulation of insulin signaling. TRB3 can directly bind to Ser/Thr protein kinase Akt, the major downstream kinase of insulin signaling. Conversely, physical exercise has been linked to improved glucose homeostasis and enhanced insulin ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Lima AF,Ropelle ER,Pauli JR,Cintra DE,Frederico MJ,Pinho RA,Velloso LA,De Souza CT

    更新日期:2009-10-01 00:00:00