Abstract:
:Many critical factors such as hypoxia, nutrient deficiency, activation of glycolytic pathway/Warburg effect contribute to the observed low pH in tumors compared to normal tissue. Studies suggest that such tumor specific acidic environment can be exploited for the development of therapeutic strategies against cancer. Independent observations show reduction in pH of mammalian cells undergoing internucleosomal DNA fragmentation and apoptosis. As such, our group has extensively demonstrated that anticancer mechanisms of different plant polyphenols involve mobilization of endogenous copper and consequent internucleosomal DNA breakage. Copper is redox active metal, an essential component of chromatin and is sensitive to subtle pH changes in its microenvironment. Here we explored whether, acidic pH promotes growth inhibition, apoptosis, and DNA damaging capacity of chemopreventive agent resveratrol. Our results reveal that growth inhibition and internucleosomal DNA fragmentation induced apoptosis in Capan-2 and Panc-28 pancreatic cancer cell lines (and not in normal HPDE cells) by resveratrol is enhanced at lower pH. Using comet assay, we further demonstrate that DNA breakage by resveratrol is enhanced with acidification. Membrane permeable copper specific chelator neocuproine (and not iron chelator orthophenanthroline) abrogated growth inhibition and apoptosis by resveratrol. Western blot results show enhanced activation of DNA laddering marker H2.aX by resveratrol at acidic pH that was reversed by neocuproine and not by orthophenanthroline. Our findings provide irrevocable proof that low pH environment can be turned into tumor weakness and assist in eradication of cancer cells by resveratrol.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Shamim U,Hanif S,Albanyan A,Beck FW,Bao B,Wang Z,Banerjee S,Sarkar FH,Mohammad RM,Hadi SM,Azmi ASdoi
10.1002/jcp.22865subject
Has Abstractpub_date
2012-04-01 00:00:00pages
1493-500issue
4eissn
0021-9541issn
1097-4652journal_volume
227pub_type
杂志文章abstract::We have studied expression and function of neurotrophins and their receptors during myogenic differentiation of C2C12 cells, a clonal cell line derived from mouse muscle that is capable of in vitro differentiation. The genes coding for nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and their commo...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199807)176:1<10::AID-JCP2>
更新日期:1998-07-01 00:00:00
abstract::In this study, we examine whether an anti-inflammatory thiourea derivative, compound #326, actions on ion channels. The effects of compound #326 on Ca2+ -activated K+ channels were evaluated by patch-clamp recordings obtained in cell-attached, inside-out or whole-cell configuration. In pituitary GH3 cells, compound #3...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25788
更新日期:2017-12-01 00:00:00
abstract::The peroxisome proliferation-activated receptor gamma (PPARgamma) is mainly expressed in the adipose tissue and integrates the control of energy, lipid, and glucose homeostasis. The present study, by means of RT-PCR, Western blot, and immunofluorescence techniques, demonstrates that human sperm express the PPARgamma. ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20807
更新日期:2006-12-01 00:00:00
abstract::Diabetic retinopathy (DR) is the most common ocular complication caused by diabetes mellitus and is the main cause of visual impairment in working-age people. Reactive gliosis and pro-inflammatory cytokine production by Müller cells contribute to the progression of DR. Melatonin is a strong anti-inflammatory hormone, ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29716
更新日期:2020-11-01 00:00:00
abstract::A method, based on the differing capacities of cells to adhere to a column of polyester fibres, has been described for separating human bone marrow cells into a nonadherent and an adherent fraction. The effect of this cell separation procedure on colony formation by erythroid progenitor cells was investigated. In cont...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040910108
更新日期:1977-04-01 00:00:00
abstract::We previously revealed that epithelial-to-mesenchymal transition (EMT) was mediated by ΔNp63β, a splicing variant of ΔNp63, in oral squamous cell carcinoma (OSCC). Recent studies have highlighted the involvement of microRNA (miRNA) in EMT of cancer cells, though the mechanism remains unclear. To identify miRNAs respon...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26267
更新日期:2018-10-01 00:00:00
abstract::We report that cultured vascular endothelial cells release into the culture medium a vasoconstrictor peptide, a substance we call an endothelium-derived constricting factor (EDCF). Conditioned medium from cultured bovine aortic and pulmonary artery endothelial cells caused sustained, dose-dependent isometric constrict...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041320210
更新日期:1987-08-01 00:00:00
abstract::Human promyelocytic leukemia (HL-60) cells display a novel voltage-dependent outward current under voltage clamp. This current is present at low levels in the proliferative state and in granulocytes derived from HL-60 cells which were induced to differentiate with retinoic acid. It is elevated in macrophages derived f...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041320227
更新日期:1987-08-01 00:00:00
abstract::Glutamine (gln) is the most abundant free amino acid in the blood. It is involved in important metabolic and biochemical processes, like cell proliferation and oxidative stress. Previous studies have demonstrated that gln concentration in human plasma decreases in several conditions such as sepsis, ischemia-reperfusio...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22928
更新日期:2012-05-01 00:00:00
abstract::The Giemsa banding pattern of the chromosomes has been analyzed in a line of transformed golden hamster cells, revertant and re-revertant cells and their tumors. The transformed and re-revertant cells were malignant in vivo and had gained an additional chromosome 5(7). Revertants with a suppression of malignancy lost ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040870112
更新日期:1976-01-01 00:00:00
abstract::The cell surface phenotype of pluripotent hemopoietic stem cells (CFU-S) and committed progenitors (CFU-C1, CFU-C2, BFU-E) of mouse bone marrow was analyzed with respect to their binding of wheat germ agglutinin (WGA) and two monoclonal antibodies, anti-GM-1.2 and anti-PGP-1. Stained cells were fractionated on the bas...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041280120
更新日期:1986-07-01 00:00:00
abstract::Hydrocortisone and dexamethasone produced a time-dependent increase [125I]epidermal growth factor [( 125I]EGF) binding in primary cultures of isolated rat hepatocytes. Maximally effective doses of glucocorticoids resulted in a 70-100% increase in binding. The effect was similar when hepatocytes were maintained on coll...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041190304
更新日期:1984-06-01 00:00:00
abstract::The role of cyclic adenosine monophosphate (cAMP) is poorly understood in the regulation of normal and abnormal hepatic cell growth. In this study, we examined the regulation of intracellular cAMP levels and its effect on nuclear cAMP responsive elements (CREs) in a rat model of hepatocellular carcinoma (HCC). Tumorig...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20474
更新日期:2006-02-01 00:00:00
abstract::Interleukin-10 (IL-10) has been shown to exert anti-inflammatory effects by suppressing macrophage proliferation and inhibiting cytokine production. In this study we show that in the presence of erythropoietin (EPO), the addition of IL-10 results in a significant dose-dependent increase in both Burst Forming Unit-Eryt...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199602)166:2<305::AID-JCP8
更新日期:1996-02-01 00:00:00
abstract::TRPV4 (transient receptor potential vanilloid 4) is a cation channel activated by hypotonicity, moderate heat, or shear stress. We describe the expression of TRPV4 during the differentiation of a corneal epithelial cell model, RCE1(5T5) cells. TRPV4 is a late differentiation feature that is concentrated in the apical ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25698
更新日期:2017-07-01 00:00:00
abstract::Insulin-like growth factor-I (IGF-I) (13 nM) can replace insulin (0.8 microM) in a serum-free medium containing epidermal growth factor (EGF) (16 nM) and dexamethasone (DEX) (140 nM) and stimulate DNA synthesis in young cultures of WI-38 cells, similar to the stimulation of serum-supplemented medium. By contrast, sene...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041330117
更新日期:1987-10-01 00:00:00
abstract::Peroxisome proliferator-activated receptor-γ coactivator-1 deficient (Pgc-1β-/- ) murine hearts model the increased, age-dependent, ventricular arrhythmic risks attributed to clinical conditions associated with mitochondrial energetic dysfunction. These were accompanied by compromised action potential (AP) upstroke ra...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27183
更新日期:2019-04-01 00:00:00
abstract::Mice of the C3H/HeJ strain exhibit low inflammatory and immunological responses to certain bacterial lipopolysaccharide (LPS) preparations. Lymphocytes from C3H/HeJ mice also show defective LPS-induced mitogenesis. We tested the colony stimulating activity (CSA) response of C3H/HeJ mice. As controls we used mice of th...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040920219
更新日期:1977-08-01 00:00:00
abstract::PI3K/AKT signaling is essential in regulating pathophysiology of osteoarthritis (OA). However, its potential modulatory role in early OA progression has not been investigated yet. Here, a mouse destabilization OA model in the tibia was used to investigate roles of PI3K/AKT signaling in the early subchondral bone chang...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26460
更新日期:2018-08-01 00:00:00
abstract::Transient receptor potential vanilloid 4 (TRPV4) channels are mechanosensitive ion channels that regulate systemic endothelial cell (EC) functions such as vasodilation, permeability, and angiogenesis. TRPV4 is expressed in retinal ganglion cells, Müller glia, pigment epithelium, microvascular ECs, and modulates cell v...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.30116
更新日期:2020-10-20 00:00:00
abstract::Multicolor immunostaining analysis is often a desirable tool in cell biology for most researchers. Nonetheless, this is not an easy task and often not affordable by many laboratories as it might require expensive instrumentation and sophisticated analysis software. Here, we describe a simple protocol for performing se...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24506
更新日期:2014-06-01 00:00:00
abstract::Epoxyeicosatrienoic acids (EETs) are arachidonic acid metabolites formed endogenously via the cytochrome P450 pathway in rat, rabbit, and human kidney. We characterized the effects of the four regioisomeric EETs on ion transport in the renal epithelial cell line, LLC-PK1. Among the EETs, 14,15-EET was the most potent ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041600109
更新日期:1994-07-01 00:00:00
abstract::Dexamethasone (Dex), a synthetic glucocorticoid (GC) with long-lasting treatment effects, has been proved to exert a modulatory effect on osteoblast proliferation and differentiation during embryonic osteogenesis. However, it is still controversial if Dex exposure influences endochondral ossification and the underlyin...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28157
更新日期:2019-01-22 00:00:00
abstract::In this present study, Oreochromis mossambicus tilapia were transferred to cold water at 12°C for various time intervals (1, 4, 8, 24, and 48 hr) and its innate immune response was analyzed by studying cellular and humoral parameters. In vivo, alternative complement pathway activity in blood plasma was rapidly increas...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28270
更新日期:2019-02-11 00:00:00
abstract::We demonstrated previously that in serum-starved MCF-7 breast cancer cell line, Ang II increased Na+/K+ATPase activity and activated the protein kinase C zeta (PKC-zeta) (Muscella et al., 2002 J Endocrinol 173:315-323; 2003 J Cell Physiol 197:61-68.). The aim of the present study was to investigate the modulation of t...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20396
更新日期:2005-11-01 00:00:00
abstract::Glycochenodeoxycholate (GCDA) is closely associated with carcinogenesis and chemoresistance of hepatocellular carcinoma (HCC). Signal transducer and activator of transcription 3 (STAT3), a transcription factor, is involved in various human tumors. Whether GCDA induces chemoresistance through STAT3 and the mechanism of...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29159
更新日期:2020-03-01 00:00:00
abstract::These are the reflections of a retired physician who was introduced to cellular physiology in 1947 at the University of Pennsylvania. I majored in Zoology. I took the required major courses, which were interesting but not exciting. I was a premedical student at the time. Fortunately I was introduced to cellular physio...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25007
更新日期:2015-11-01 00:00:00
abstract::A sensitive serum-free culture system was used to demonstrate that cells derived from normal human skin release soluble mediators which can modulate keratinocyte, melanocyte, and fibroblast growth in vitro. In M199 supplemented with epidermal growth factor, hydrocortisone, insulin, transferrin, triidothyronine, bovine...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041170215
更新日期:1983-11-01 00:00:00
abstract::In order to study the generation, factors controlling endogenous folate pools, and their functional importance, Friend erythroleukemia cells were grown in media containing 100; 1,000; and 10,000 ng/ml of tritiated pteroylglutamic acid (3H)PteGlu1 and then studied in unlabeled media with varying amounts of PteGlu1. The...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041140216
更新日期:1983-02-01 00:00:00
abstract::The human neuroblastoma cell line SK-N-BE, after incubation with 10 microM retinoic acid (RA) or 20 nM phorbol 12-myristate 13-acetate (PMA), underwent biochemical and morphological signs of differentiation within 10-14 days. In parallel, SK-N-BE cells produced significantly higher amounts of nitric oxide (NO) in comp...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199801)174:1<99::AID-JCP11
更新日期:1998-01-01 00:00:00