Abstract:
:Epoxyeicosatrienoic acids (EETs) are arachidonic acid metabolites formed endogenously via the cytochrome P450 pathway in rat, rabbit, and human kidney. We characterized the effects of the four regioisomeric EETs on ion transport in the renal epithelial cell line, LLC-PK1. Among the EETs, 14,15-EET was the most potent inhibitor of 86Rb uptake. Its effect was concentration-dependent (IC50 = 75 nM) and stereoselective to the 14S, 15R-EET. Experiments measuring 14,15-EET-induced 86Rb uptake inhibition in the presence of inhibitors of Na(+)-K(+)-ATPase activity (ouabain), Na(+)-K(+)-Cl- cotransporter (furosemide), and Na(+)-H+ exchanger (amiloride) suggested that 14,15-EET inhibits ion transport via an amiloride-sensitive mechanism. These results, together with previous reports demonstrating their endogenous production in the kidney, suggest an important role for EETs, specifically 14,15-EET, in the regulation of ion and water reabsorption in the kidney and implicate their function in renal pathophysiology.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Staudinger R,Escalante B,Schwartzman ML,Abraham NGdoi
10.1002/jcp.1041600109subject
Has Abstractpub_date
1994-07-01 00:00:00pages
69-74issue
1eissn
0021-9541issn
1097-4652journal_volume
160pub_type
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