Evidence suggesting a stimulatory role for interleukin-10 in erythropoiesis in vitro.


:Interleukin-10 (IL-10) has been shown to exert anti-inflammatory effects by suppressing macrophage proliferation and inhibiting cytokine production. In this study we show that in the presence of erythropoietin (EPO), the addition of IL-10 results in a significant dose-dependent increase in both Burst Forming Unit-Erythroid (BFU-E) and Colony Forming Unit-Erythroid (CFU-E) colony growth in both serum-containing and serum-free murine cultures in vitro. IL-10 acts at the later stages of erythroid cell proliferation and differentiation as the increase in colony number was greater in CFU-E than in BFU-E, and was similar when IL-10 was added to BFU-E cultures at the time of culture initiation as when its addition to culture was delayed for 7 days. Furthermore, no increase in BFU-E colony number was noted when IL-10, added at the time of culture initiation, was neutralized by the addition to culture of a monoclonal anti-IL-10 antibody up to 7 days later. The increases in BFU-E by IL-10 addition were not the result of prolongation of BFU-E colony lifespan, which was not significantly different in IL-10 treated and control cultures, respectively. Rather IL-10 stimulated the proliferation of erythroid clusters that were now large enough to be recognized as colonies. IL-10-induced stimulation of erythropoiesis appeared to be independent of its inhibitory effects on macrophage function, as stimulation of erythroid colony growth was similar in macrophage-containing and depleted cultures. Studies to determine if the IL-10 effect was direct or indirect yielded equivocal results. A limiting dilution assay suggested a direct effect. However, a log/log dose response curve with IL-10 did not pass through the origin suggesting an indirect effect. These studies indicate that IL-10 acts synergistically with EPO to significantly increase stimulation of erythroid differentiation and proliferation in vitro and may be involved in the regulation of normal erythropoiesis in vivo.


J Cell Physiol


Wang CQ,Udupa KB,Lipschitz DA




Has Abstract


1996-02-01 00:00:00














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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Matheson LA,Santerre JP,Labow RS

    更新日期:2004-04-01 00:00:00

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Antelman J,Manandhar G,Yi YJ,Li R,Whitworth KM,Sutovsky M,Agca C,Prather RS,Sutovsky P

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  • The effects and mechanisms of myeloid differentiation protein 2 on intestinal mucosal permeability in mice with chronic colitis.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Han C,Guan Q,Guo L,Yang Y,Ruan S,Zhang X

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  • Interleukin-6 increases adrenal androgen release by regulating the expression of steroidogenic proteins in NCI-H295R cells.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Wei X,Zhang J,Peng W,Xu H,Wei Z,Pang L,Liu J,Wang T

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ehrhardt AG,Sluder G

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: De Cristan G,Morbidelli L,Alessandri G,Ziche M,Cappa AP,Gullino PM

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    authors: Yu X,Wei L,Lu P,Shen T,Liu X,Li T,Zhang B,Yu H,Zhu D

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    authors: Kelemen E,Gulya E,Vass K

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    authors: Chen JC,Shao ZM,Sheikh MS,Hussain A,LeRoith D,Roberts CT Jr,Fontana JA

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    authors: Piiper A,Stryjek-Kaminska D,Gebhardt R,Zeuzem S

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    journal_title:Journal of cellular physiology

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  • Non-Metabolic Role of PKM2 in Regulation of the HIV-1 LTR.

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    journal_title:Journal of cellular physiology

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    authors: Petch D,Butler M

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Charollais RH,Buquet C,Mester J

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    pub_type: 杂志文章


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