Abstract:
:Abdominal aortic aneurysm (AAA) is a potentially lethal disease featured by focal dilatation in the aorta. The transition of vascular smooth muscle cells (SMCs) from a contractile/differentiated to a synthetic/dedifferentiated phenotype is considered to contribute to AAA formation and expansion. Our previous gene microarray data showed that Ventricular Zone Expressed PH Domain Containing 1 (VEPH1) expression increased in angiotensin II (Ang II)-infused aortic tissues. This study was thus performed to further explore the role of VEPH1. Herein, we first demonstrate that VEPH1 increases in the SMCs of Ang II-treated abdominal aortas. As in vivo, Ang II also upregulated VEPH1 expression in cultured hAoSMCs. The dedifferentiation of human aortic SMCs (hAoSMCs) was induced by a 24-hr stimulation of Ang II (1 μM)-the expression of contractile SMC markers, MYH11 and α-smooth muscle actin (α-SMA) decreased and that of synthetic markers, proliferating cell nuclear antigen and Vimentin increased. Inhibition of VEPH1 prevented Ang II-induced pathological dedifferentiation of hAoSMCs as indicated by the restored expression of MYH11 and α-SMA. In contrast, the forced overexpression of VEPH1 aggravated Ang II's effects. Furthermore, we demonstrated that VEPH1 and transforming growth factor-β1 (TGF-β1), a key regulator responsible for vascular SMC differentiation, negatively regulated each other's transcription. In contrast to VEPH1 silencing, its overexpression inhibited recombinant TGF-β1-induced increases in MYH11 and α-SMA and suppressed Smad3 phosphorylation and nuclear accumulation. Collectively, our study demonstrates that VEPH1 elevation promotes the synthetic phenotype switching of AoSMCs and suppressed the TGF-β1/Smad3 signaling pathway. Identification of VEPH1 as a pathogenic molecule for AAA formation provides novel insights into this disease.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Shi X,Xu C,Li Y,Wang H,Ma W,Tian Y,Yang H,Li Ldoi
10.1002/jcp.29736subject
Has Abstractpub_date
2020-12-01 00:00:00pages
9336-9346issue
12eissn
0021-9541issn
1097-4652journal_volume
235pub_type
杂志文章abstract::The exposure to non-thermal microwave electromagnetic field (MW-EMF) at 1.95 MHz, a frequency used in mobile communication, affects the refolding kinetics of eukaryotic proteins (Mancinelli et al., 2004). On these basis we have evaluated the in vivo effect of MW-EMF in human epidermoid cancer KB cells. We have found t...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20327
更新日期:2005-08-01 00:00:00
abstract::Progesterone is an endogenous immunomodulator and can suppress T-cell activation during pregnancy. We have previously shown that the non-genomic effects of progesterone, especially acidification, are exerted via plasma membrane sites and suppress cellular genomic responses to mitogens. This study aimed to show that ac...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20962
更新日期:2007-05-01 00:00:00
abstract:OBJECTIVE:Disc degeneration is the common life-threatening disease characterized by flank pain. The gene expression of insulin-like growth factor binding protein 3 (IGFBP3) is increased in patients with disc degeneration, however, its mechanism is still unknown. This study aimed to investigate the influence of IGFBP3 g...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27613
更新日期:2019-06-01 00:00:00
abstract::Apoptosis constitutes a response of organisms to various physiological or pathological stimuli, and to different stresses. The ability of thermotolerance induced at a mild temperature of 40 degrees C to protect against activation of the apoptotic cascade by heat shock was investigated. When Chinese hamster ovary and h...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20386
更新日期:2005-10-01 00:00:00
abstract::Obesity is a risk factor for endometrial cancer in pre- and post-menopausal women. Leptin, an adipocyte-derived hormone, in addition to the control weight homeostasis, is implicated in multiple biological actions. A recent study demonstrated that leptin promotes endometrial cancer growth and invasiveness through STAT/...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21622
更新日期:2009-03-01 00:00:00
abstract::Male breast cancer (MBC) is an uncommon malignancy. We have previously reported that the expression of the Hippo transducers TAZ/YAP and their target CTGF was associated with inferior survival in MBC patients. Preclinical evidence demonstrated that Axl is a transcriptional target of TAZ/YAP. Thus, we herein assessed A...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25745
更新日期:2017-08-01 00:00:00
abstract::Hyaluronate degradation was examined in cultures of vascular wall cells (bovine aortic endothelial cells, rat aortic smooth muscle cells) and in nonvascular cells (chick embryo fibroblasts). The three cell types examined all produced hyaluronidase activity in culture which had a strict acidic pH requirement for activi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041330210
更新日期:1987-11-01 00:00:00
abstract::Evolving data suggest that marrow hematopoietic stem cells show reversible changes in homing, engraftment, and differentiation phenotype with cell cycle progression. Furthermore, marrow stem cells are a cycling population. Traditional concepts hold that the system is hierarchical, but the information on the lability o...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21273
更新日期:2008-03-01 00:00:00
abstract::Nuclear factor-κB (NF-κB), a family of master regulated dimeric transcription factors, signaling transduction pathways are active players in the cell signaling that control vital cellular processes, including cell growth, proliferation, differentiation, apoptosis, morphogenesis, angiogenesis, and immune responses. Nev...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.28249
更新日期:2019-02-11 00:00:00
abstract::Numerous studies have suggested that urothelial cancer-associated 1 (UCA1) acts as a suppressor gene affecting cell proliferation and migration. However, the biological role and the potential mechanism of UCA1 in the progression of pre-eclampsia (PE) remains unclear. The UCA1 level was markedly upregulated in PE pregn...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29643
更新日期:2020-10-01 00:00:00
abstract::Myocardium infarction is one of the most deathly cardiovascular diseases. It is characterized by myocardium ischemia as a result of nutrients depletion and hypoxia. The cell can respond to this injury by autophagy or apoptosis, which determines the evolution and possible recovery of the myocardium infarction. Lipids p...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25344
更新日期:2016-10-01 00:00:00
abstract::The role of the plasma membrane potential (delta psi p) in the commitment to differentiation of murine erythroleukemia (MEL) cells has been studied by analyzing the ionic basis and the time course of this potential in the absence or the presence of different types of inducers. delta psi p was determined by measuring t...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041320302
更新日期:1987-09-01 00:00:00
abstract::We have previously demonstrated that kidney embryonic structures are present in rats, and are still developing until postnatal Day 20. Consequently, at postnatal Day 10, the rat renal papilla contains newly formed collecting duct (CD) cells and others in a more mature stage. Performing primary cultures, combined with ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28016
更新日期:2019-08-01 00:00:00
abstract::The possibility that one or both of the synthetic triamines, 1,3,6-triaminohexane and 1,4,7-triaminoheptane, could substitute for the naturally occurring polyamines in the growth of SV-3T3 cells was investigated. It was found that these triamines did lead to a restoration of growth in cells in which spermidine content...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041270219
更新日期:1986-05-01 00:00:00
abstract::The relationship between replication and the synthesis of matrix sulfated proteoglycans was investigated with fetal rat chondrocytes grown in monolayer culture. The effect of N6 O2' dibutyryl adenosine 3', 5' cyclic monophosphate (DBcAMP), adenosine 3', 5' cyclic monophosphate (cAMP), 8 Bromo adenosine 3', 5' cyclic m...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041000107
更新日期:1979-07-01 00:00:00
abstract::Neurofibromatosis type 1 (NF1) is characterized by the formation of neurofibromas, benign tumors of the peripheral nerve consisting essentially of Schwann cells, which can sometimes turn malignant to form neurofibrosarcomas. The mechanism of progression toward a malignant phenotype remains largely unknown. In this rep...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199811)177:2<334::AID-JCP1
更新日期:1998-11-01 00:00:00
abstract::The effects of kinin B1 receptor (B1 R) deletion were examined on femur bone regeneration in streptozotocin (STZ)-type 1 diabetes. Diabetes induction in wild-type C57/BL6 (WTC57BL6) mice led to decrease in body weight and hyperglycemia, compared to the non-diabetic group of the same strain. The lack of B1 R did not af...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25034
更新日期:2015-12-01 00:00:00
abstract::Mast cells are central regulators of allergic inflammation through production of various chemical mediators and cytokines. Bacterial infection occasionally worsens allergic inflammation. Although the exact mechanism of this phenomenon remains unclear, we have previously reported that LPS stimulates mast cells to produ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21101
更新日期:2007-10-01 00:00:00
abstract::Both mammary gland development and mammary carcinogenesis involve extensive remodeling of the mammary gland extracellular matrix. The expression of four membrane-type matrix metalloproteinases (MT-MMPs) with matrix remodeling potential in development and tumorigenesis was evaluated by in-situ hybridization on mouse ma...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20385
更新日期:2005-10-01 00:00:00
abstract::rac-1-O-Myristoyl-2-O-acetylglycerol, rac-1-O-palmitoyl-2-O-acetylglycerol, and rac-1-O-oleoyl-2-O-acetylglycerol acted like phorbol myristate acetate and mezerein in stimulating human neutrophil aggregation. Responses to these agents were equally influenced by cytochalasin B, extracellular calcium and magnesium, arac...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041250204
更新日期:1985-11-01 00:00:00
abstract::High temperature requirement A1 (HTRA1) is a serine protease playing a modulatory role in various cell processes, particularly in the regulation of transforming growth factor-β (TGF-β) signaling. A deleterious role in late-onset cerebral small vessel diseases (CSVDs) of heterozygous HTRA1 mutations, otherwise causativ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29609
更新日期:2020-10-01 00:00:00
abstract::Hyperthermia is a clinical sign of inflammation and constitutes in itself an adaptive defense mechanism. The fibrinolytic system, a highly regulated proteolytic system, is involved in inflammatory processes. Plasminogen activator inhibitor 1 (PAI-1) is the principal inhibitor of the two activators of the fibrinolytic ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041480217
更新日期:1991-08-01 00:00:00
abstract::CCN1 is highly expressed in cancer cells and has been identified in the secretome of bone marrow-derived mesenchymal stem cells (BM-MSC). Although secreted CCN1 is known to promote angiogenesis, its underlying mechanism remains unclear. Here, we examined whether our recently-established tonsil-derived MSC (T-MSC) secr...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24690
更新日期:2015-01-01 00:00:00
abstract::The cardiac glyoside, ouabain, normally kills HeLa cells at concentrations of about 10-7 M or greater. By treating a population of HeLa cells with increasingly higher concentrations of the drug, a vaiant population was obtained of HeLa cells capable of growing in medium containing 10-4 M ouabain. Inhibition of volume ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040850114
更新日期:1975-02-01 00:00:00
abstract::There is compelling in vitro and in vivo evidence to implicate mast cells in the development of fibrosis. However, an important question remains as to the mechanisms by which mast cells mediate fibrosis. Recent evidence from our laboratory (Gruber et al., 1997, J. Immunol. , 158:2310-2317) has revealed that tryptase, ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199911)181:2<312::AID-JCP1
更新日期:1999-11-01 00:00:00
abstract::Cadmium (Cd) is a toxic metal with multiple effects on cell signaling and cell death. We studied the effects of Cd(2+) on quiescent mouse mesangial cells in serum-free conditions. Cadmium induces cell death over 6 h through annexin V+ states without or with causing uptake of propidium iodide, termed apoptotic and apop...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21499
更新日期:2008-11-01 00:00:00
abstract::In the present study, we have employed a unique breast cancer cell line (Met-1, which was derived from a high metastatic potential tumor in transgenic mice expressing polyomavirus middle T oncogene) to study the role of CD44 variant isoform(s) in the regulation of metastatic breast tumor cell behavior. The results of ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199807)176:1<206::AID-JCP2
更新日期:1998-07-01 00:00:00
abstract::Hemopoietic cell proliferation is controlled by a set of polypeptide growth factors and regulatory molecules that bind to cell surface receptors inducing cellular responses. Maintenance of a viable state, cell growth, DNA synthesis and mitosis are basic properties of proliferating cells, but links between growth facto...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041630306
更新日期:1995-06-01 00:00:00
abstract::RB loss has long been recognized as the causative genetic alteration underlying retinoblastoma but it is increasingly evident that other alterations are required for the tumor to develop. Therefore, we set out to identify additional inheritable susceptibility markers and new potential preventive and therapeutic target...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22019
更新日期:2010-04-01 00:00:00
abstract::P2Y(2) receptor expression is increased in intestinal epithelial cells (IECs) during inflammatory bowel diseases (IBDs). In this context, P2Y(2) stimulates PGE(2) release by IECs, suggesting a role in wound healing. For this study, we have used the non-cancerous IEC-6 cell line. IEC-6 cell migration was determined usi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24109
更新日期:2013-01-01 00:00:00