Abstract:
:Proteinuria in diabetic nephropathy has been correlated with reduction in heparan sulfate proteoglycan (HSPG) content of the glomerular basement membrane. We have previously shown that the underlying mechanism probably involves reduction in the synthesis by glomerular epithelial cells. In this study we explored whether high glucose medium regulates basement membrane HSPG gene expression. Northern analysis demonstrated that rat glomerular epithelial cells in vitro constitutively express mRNA for basement membrane HSPG, similar to that observed in rat kidney glomerulus. RNase protection assay showed that incubation of glomerular epithelial cells with 30 mM glucose for 24 h and 7 days resulted in reduction in HSPG mRNA abundance. The decrease in mRNA abundance correlated with reduction in the synthesis of 35SO4-labeled basement membrane HSPG as measured by immunoprecipitation. Reduction in synthesis of HSPG could not be entirely accounted for by decrease in mRNA abundance, suggesting both transcriptional and posttranscriptional mechanisms may be involved in reduction of glomerular basement membrane HSPG synthesis by glomerular epithelial cells in diabetic nephropathy.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Kasinath BS,Grellier P,Choudhury GG,Abboud SLdoi
10.1002/(SICI)1097-4652(199604)167:1<131::AID-JCP1subject
Has Abstractpub_date
1996-04-01 00:00:00pages
131-6issue
1eissn
0021-9541issn
1097-4652pii
10.1002/(SICI)1097-4652(199604)167:1<131::AID-JCP1journal_volume
167pub_type
杂志文章abstract::The effect of retinoic acid on human fibroblasts was studied in a cell culture model of chronologic aging and photoaging. During early exponential phase, all trans-retinoic acid significantly stimulated growth rate of adult arm-derived dermal fibroblasts but not of newborn or adult foreskin-derived fibroblasts. Retino...
journal_title:Journal of cellular physiology
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journal_title:Journal of cellular physiology
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journal_title:Journal of cellular physiology
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pub_type: 杂志文章,评审
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
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更新日期:1996-12-01 00:00:00
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journal_title:Journal of cellular physiology
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journal_title:Journal of cellular physiology
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journal_title:Journal of cellular physiology
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更新日期:2011-11-01 00:00:00
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journal_title:Journal of cellular physiology
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