Abstract:
:Rat 3Y1 cells arrested at early S by hydroxyurea traversed the remainder of S and G2 and completed mitosis after removal of the drug, irrespective of the absence of serum from the culture medium. When cells were deprived of serum for a period between early S and mitosis after removal of hydroxyurea, the cells delayed entry into S in the presence of serum in the second generation for the time length approximately equal to that of serum deprivation. When mitotic cells, which had been continuously exposed to serum after removal of hydroxyurea, were deprived of serum for the next 24 hours and then were reexposed to serum, the cells delayed entry into S for more than 24 hours (more than the time length of serum deprivation). On the other hand, the cells already deprived of serum between early S and G2 in the first generation were less delayed in entry into S after postmitotic 24-hour serum deprivation than were the cells exposed to serum between early S and G2 in the first generation. These results suggest that serum-dependent events continue to occur in the first generation for on-time entry into S in the next generation, and that these premitotic events (the potential for entry into S) decay if serum is absent for a long period of time after mitosis.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Okuda A,Kimura Gdoi
10.1002/jcp.1041100308subject
Has Abstractpub_date
1982-03-01 00:00:00pages
267-70issue
3eissn
0021-9541issn
1097-4652journal_volume
110pub_type
杂志文章abstract::The Na+ pump (Na+, K+-ATPase) has been implicated in the regulation of many cellular functions, including cell volume regulation. The effects of inhibiting Na+ pump activity on cell volume and taurine efflux were evaluated in the human neuroblastoma cell line CHP-100. Cell volume changes monitored with the Coulter Mul...
journal_title:Journal of cellular physiology
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更新日期:1983-09-01 00:00:00
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更新日期:2005-07-01 00:00:00
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journal_title:Journal of cellular physiology
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更新日期:1979-07-01 00:00:00
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更新日期:1991-12-01 00:00:00
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journal_title:Journal of cellular physiology
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更新日期:2008-03-01 00:00:00
abstract::The increased levels of c-Myc protein observed previously in an ovarian carcinoma cell line stably transfected to express HER2 has suggested a role for the HER2 pathway in c-Myc expression. Analysis of HER2-transfected cells stimulated with heregulin beta1 (HRG) revealed increased c-Myc protein levels but not a corres...
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更新日期:2018-05-01 00:00:00
abstract::Ack1 (also known as ACK, TNK2, or activated Cdc42 kinase) is a structurally unique non-receptor tyrosine kinase that is expressed in diverse cell types. It integrates signals from plethora of ligand-activated receptor tyrosine kinases (RTKs), for example, MERTK, EGFR, HER2, PDGFR and insulin receptor to initiate intra...
journal_title:Journal of cellular physiology
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更新日期:2010-08-01 00:00:00
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更新日期:1989-07-01 00:00:00
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journal_title:Journal of cellular physiology
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更新日期:2018-09-01 00:00:00
abstract::We have previously shown that extracellular ATP acts as a mitogen via protein kinase C (PKC)-dependent and independent pathways (Wang, D., Huang, N., Gonzalez, F.A., and Heppel, L.A. Multiple signal transduction pathways lead to extracellular ATP-stimulated mitogenesis in mammalian cells. I. Involvement of protein kin...
journal_title:Journal of cellular physiology
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doi:10.1002/jcp.1041460320
更新日期:1991-03-01 00:00:00
abstract::Recently, ATP has gained much interest as an extracellular messenger involved in the communication of calcium signals between cells. The mechanism of ATP release is, however, still a matter of debate. In the present study we investigated the possible contribution of connexin hemichannels or ion channels in the release...
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doi:10.1002/jcp.10365
更新日期:2003-11-01 00:00:00