Abstract:
:Normal human keratinocytes (NHK) were cultured in serum-free medium, containing low (0.1 mM) or high (2 mM) calcium, to obtain proliferating and differentiating cultures, respectively. Proteoglycan (PG) synthesis of proliferating and differentiating NHK was investigated. Cultures were labeled with 35S-sulfate, and the PGs were extracted from medium and cell layer. The newly synthesized PGs were isolated by ion-exchange chromatography on a column of DEAE-Sephacel. The molecular properties of the PGs and the size and composition of glycosaminoglycans (GAGs) were determined. In general, the PGs are relatively small size (Mr 70,000-120,000). The PGs of proliferating cultures are larger in molecular size than the PGs of differentiating cultures, and this is due to the degradation of the GAG chains. The molecular weight of the GAG chains of proliferating NHK ranged from 4,800 to 22,000, and the range for GAGs from differentiating cultures varied from 2,800 to 9,600. By compositional analysis, these PGs proved to contain heparan sulfate, chondroitin sulfate, and dermatan sulfate as determined by nitrous acid degradation, and chondroitinase ACII and ABC digestion. No significant differences were found in the overall GAG composition of the medium secreted PGs of proliferating and differentiating cultures. In contrast, cell-associated PGs of differentiating cells had higher levels of heparan sulfate than those of proliferating cells.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Rahemtulla F,Moorer CM,Wille JJ Jrdoi
10.1002/jcp.1041400113subject
Has Abstractpub_date
1989-07-01 00:00:00pages
98-106issue
1eissn
0021-9541issn
1097-4652journal_volume
140pub_type
杂志文章abstract::The p53 protein directly regulates the expression of the WAF1 (wild-type p53-activated fragment 1) protein which is a cyclin-dependent kinase inhibitor (CDK1). DNA damaging agents such as ionizing or UV radiation, and some chemical agents induce WAF1 in wild-type p53 containing cells, thereby halting cell cycle progre...
journal_title:Journal of cellular physiology
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
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更新日期:1988-07-01 00:00:00
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更新日期:2013-02-01 00:00:00
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journal_title:Journal of cellular physiology
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更新日期:2012-08-01 00:00:00
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journal_title:Journal of cellular physiology
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更新日期:2012-05-01 00:00:00
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更新日期:2018-04-01 00:00:00
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更新日期:2015-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-06-01 00:00:00