当前位置： SCI文献检索 > JOURNAL OF CELLULAR PHYSIOLOGY期刊下所有文献 > Persistent effects of phorbol 12-myristate 13-acetate: possible implication of vesicle traffic.

Persistent effects of phorbol 12-myristate 13-acetate: possible implication of vesicle traffic.

Abstract：

:Relative to their normal counterparts, transformed epithelial cells have a distinctive and quantifiable three-dimensional shape. Biophysical and mathematical methods are used to distinguish these extremes in cells from two lines, cultured from rat liver and tracheal epithelium, respectively. Cells adopted a more transformed-looking configuration transiently when exposed to phorbol 12-myristate 13-acetate (PMA) (Plummer and Heckman, [1990] Exp. Cell Res., 188:66-74). The purpose of the present work was to dissect the physiological processes involved in the shape change. Ruffling activity, known to be PMA-stimulated in other cells, was investigated. Although the ruffles appeared less robust than normal, PMA stimulated ruffling activity over a 5 h period. The number of sites where ruffling was initiated declined by 5 h, however, and suppression was seen by 10 h. Cells from both lines adopted the transformed shape configuration when exposed for 2 h to monensin. When the subset of shape features changed by this treatment was compared with those originally changed during transformation, it was found that monensin-treated cells mimicked the features of transformed cells. Its effect on ruffling was, however, unlike PMA's. Thus, the phenotype was unlikely to arise from ruffling itself but might be a process driven by ruffling. Chloroquine also stimulated cells to assume characteristics of transformed cells. Since both it and monensin could interfere with endosomes and with the processing of endocytosed contents, this was a likely site of action. Experiments were done to determine whether PMA also affected the processing of extracellular fluids. When the accumulation of horseradish peroxidase (HRP) was measured, the rate was found to be higher in PMA-treated cells from 5 min, the earliest time assayed, onward. The results suggest that the transformed type of cell in these cell lines showed a constitutive dilation and/or reorganization of some portion of the endosomal pathway.

journal_name

J Cell Physiol

journal_title

Journal of cellular physiology

authors

Heckman CA,Plummer HK 3rd,Runyeon CS

doi

10.1002/(SICI)1097-4652(199601)166:1<217::AID-JCP2

subject

Has Abstract

pub_date

1996-01-01 00:00:00

pages

217-30

issue

eissn

0021-9541

issn

1097-4652

pii

10.1002/(SICI)1097-4652(199601)166:1<217::AID-JCP2

journal_volume

166

pub_type

杂志文章

在线工具

Persistent effects of phorbol 12-myristate 13-acetate: possible implication of vesicle traffic.