Proper E-cadherin membrane location in colon requires Dab2 and it modifies by inflammation and cancer.

Abstract:

:We reported that Disabled-2 (Dab2) is located at the apical membrane in suckling rat intestine. Here, we discovered that, in colon of suckling and adult mouse and of adult human, Dab2 is only at lateral crypt cell membrane and colocalized with E-cadherin. Dab2 depletion in Caco-2 cells led to E-cadherin internalization indicating that its membrane location requires Dab2. In mice, we found that 3 days of dextran sulfate sodium-induced colitis increased Dab2/E-cadherin colocalization, which was decreased as colitis progressed to 6 and 9 days. In agreement, Dab2/E-cadherin colocalization increased in human mild and severe ulcerative colitis and in polyps, being reduced in colon adenocarcinomas, which even showed epithelial Dab2 absence and E-cadherin delocalization. Epithelial Dab2 decrement preceded that of E-cadherin. We suggest that Dab2, by inhibiting E-cadherin internalization, stabilizes adherens junctions, and its absence from the epithelium may contribute to development of colon inflammation and cancer.

journal_name

J Cell Physiol

authors

Vazquez-Carretero MD,García-Miranda P,Balda MS,Matter K,Ilundáin AA,Peral MJ

doi

10.1002/jcp.29917

subject

Has Abstract

pub_date

2021-02-01 00:00:00

pages

1083-1093

issue

2

eissn

0021-9541

issn

1097-4652

journal_volume

236

pub_type

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