Abstract:
BACKGROUND:Acute pancreatitis is among the few inflammatory diseases for which no specific pharmacological treatment is available. It has previously been shown that bile acids alter pancreatic ductal secretion and these effects are probably involved in the pathogenesis of bile-induced pancreatitis. OBJECTIVE:To understand the mechanism responsible for bile-induced hypersecretion and, in particular, to identify the molecular target for bile acids in native pancreatic duct epithelial cells (PDECs). METHODS:Patch clamp recordings and spectrofluorimetry were used to measure whole cell currents and rates of HCO₃⁻ secretion, respectively, from isolated guinea pig pancreatic ducts. Expression of ion channels and receptors was investigated by immunohistochemistry/immunofluorescence of intact pancreatic tissue. RESULTS:Exposing PDECs to chenodeoxycholate (CDC, 100 μM) reversibly increased whole cell K(+) currents and hyperpolarised cell membrane potential. Bile acid-stimulated K(+) currents were inhibited by Ba²(+) (2 mM), iberiotoxin (100 nM), and suppressed by strong intracellular Ca²(+) buffering. Luminally applied iberiotoxin also blocked CDC-stimulated HCO₃⁻secretion from microperfused ducts; however, the inhibitor did not influence the stimulatory effect of secretin, carbachol or luminally applied ATP. The specific large-conductance Ca²(+)-activated potassium (BK) channel activator, NS11021, induced a similar increase in HCO₃⁻secretion to CDC. Immunohistochemical analysis showed strong BK channel protein expression on the apical membrane of PDECs, while the G-protein-coupled bile acid receptor-1 was not detected in PDECs, but was present in acinar cells. CONCLUSION:It was shown for the first time that BK channels (i) are expressed at the apical membrane of guinea pig PDECs; (ii) have a crucial role in regulating HCO₃⁻ secretion and (iii) are also essential for the bile acid-induced hypersecretion and, therefore, underlie the response of the pancreas to this noxious agent.
journal_name
Gutjournal_title
Gutauthors
Venglovecz V,Hegyi P,Rakonczay Z Jr,Tiszlavicz L,Nardi A,Grunnet M,Gray MAdoi
10.1136/gut.2010.214213subject
Has Abstractpub_date
2011-03-01 00:00:00pages
361-9issue
3eissn
0017-5749issn
1468-3288pii
gut.2010.214213journal_volume
60pub_type
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