Abstract:
BACKGROUND AND AIMS:Crohn's disease (CD) and ulcerative colitis (UC) are chronic multifactorial inflammatory bowel diseases (IBDs) with unknown aetiology, but a deregulated mucosal immune response to gut-derived bacterial antigens is thought to be involved. Toll-like receptor ligands, especially lipopolysaccharide (LPS), contribute to the maintenance of the disease. It has previously been shown that the enzyme alkaline phosphatase (AP) is able to detoxify LPS, and the aim of this study was to examine a possible role in IBDs. METHODS:Intestinal AP (iAP) mRNA expression and LPS dephosphorylation in intestinal biopsies of control subjects and patients with IBD were examined, and the effect of orally administered iAP tablets on the progression of dextran sodium sulfate-induced colitis in rats was subsequently studied. RESULTS:In healthy persons, iAP mRNA and enzyme activity was high in the ileum relative to the colon. In patients with UC and CD, iAP mRNA expression was found to be markedly reduced when inflamed tissue was compared with non-inflamed tissue. Oral administration of iAP tablets to colitic rats resulted in a significant attenuation of colonic inflammation as reflected by reduced mRNA levels for tumour necrosis factor alpha, interleukin 1 beta, interleukin 6 and inducible nitric oxide synthase NOS (iNOS), a reduced iNOS staining and inflammatory cell influx, and a significantly improved morphology of the intestinal wall. CONCLUSIONS:The present study shows that epithelial iAP mRNA expression is reduced in patients with UC and CD. The rat model demonstrates that oral administration of active iAP enzymes in the intestinal tract results in a significant reduction of inflammation. This provides new insight on IBD pathology and a novel treatment approach to this severe inflammatory disease.
journal_name
Gutjournal_title
Gutauthors
Tuin A,Poelstra K,de Jager-Krikken A,Bok L,Raaben W,Velders MP,Dijkstra Gdoi
10.1136/gut.2007.128868subject
Has Abstractpub_date
2009-03-01 00:00:00pages
379-87issue
3eissn
0017-5749issn
1468-3288pii
gut.2007.128868journal_volume
58pub_type
杂志文章相关文献
GUT文献大全abstract::Carbenoxolone (Biogastrone, Berk) has been shown to reduce the peptic activity and total acidity of gastric juice obtained from anaesthetized pylorus-ligated rats without affecting significantly the volume of gastric juice secreted or the K(+) concentration. Glycyrrhetinic acid was less potent in reducing peptic activ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.11.4.344
更新日期:1970-04-01 00:00:00
abstract::Marked decreases in 24-hour Heidenhain pouch secretion, lasting from nine to 18 days, were observed in five mongrel bitches during periods of naturally occurring oestrus. Neither oophorectomy nor control celiotomy caused significant change in Heidenhain pouch secretion in bitches who were not experiencing oestrus. The...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.11.10.855
更新日期:1970-10-01 00:00:00
abstract::Infection with the delta agent can only occur in the context of coexistent hepatitis B virus infection. We describe a patient in whom the clinical features of acute delta hepatitis developed when seroconversion from hepatitis B surface antigen to antibody had already occurred and diagnosis of recent acute hepatitis B ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.26.2.212
更新日期:1985-02-01 00:00:00
abstract::The influence of ischaemia and revascularisation on lipid peroxidation and phospholipid metabolism in the rat small intestinal mucosa was investigated. Two hours of total ischaemia followed by five minutes of revascularisation caused not only accumulation of malondialdehyde in the mucosa, but also increased activity o...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.28.11.1445
更新日期:1987-11-01 00:00:00
abstract:BACKGROUND:The diagnosis and classification of oesophageal motility disorders is currently based on assessment of the phasic contractile activity of the oesophagus. Tonic muscular contraction of the oesophageal body (oesophageal tone) has not been well characterised. AIM:To quantify oesophageal tonic activity in healt...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.41.3.291
更新日期:1997-09-01 00:00:00
abstract:BACKGROUND:Experimental, clinical, and epidemiological studies have implicated mitogenic metabolites of arachidonic acid such as prostaglandin E(2) (PGE(2)) in colorectal carcinogenesis. Recently, cyclooxygenase 2 (COX-2) which catalyses the conversion of arachidonic acid to PGE(2), has displayed increased levels in hu...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.45.5.730
更新日期:1999-11-01 00:00:00
abstract:BACKGROUND:Integrins mediate cell matrix adhesion and regulate cell growth and survival. In colonic epithelial cells, alpha(2)beta(1) integrin controls glandular differentiation and proliferation. Butyrate stimulates differentiation and induces apoptosis in vitro. AIMS:We investigated whether butyrate induction of apo...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.52.5.729
更新日期:2003-05-01 00:00:00
abstract:BACKGROUND:Lamina propria (LPLs) and intraepithelial (IELs) lymphocytes are markedly increased in coeliac mucosa, and are thought to play a crucial role in the generation of villous atrophy in coeliac disease (CD). However, the mechanisms by which they mediate the killing of enterocytes in this condition are still poor...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.49.3.380
更新日期:2001-09-01 00:00:00
abstract::The injection of rabbits' ileum with homogenates of both normal and Crohn's affected human bowel tissue gave Crohn's-like changes in 11 of 27 animals after six months, but 12 months after injection the rabbit bowel had reverted to normal. The addition of ampicillin to the homogenates prevented the appearance of these ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.18.5.360
更新日期:1977-05-01 00:00:00
abstract::The peroxisome proliferator activated receptor gamma(PPARgamma) is a nuclear receptor highly expressed in the colon and playing a key role in bacterial induced inflammation. Regulation of colon inflammation by this receptor has been well demonstrated in many experimental models of colitis but also in patients with ulc...
journal_title:Gut
pub_type: 杂志文章,评审
doi:10.1136/gut.2006.093484
更新日期:2006-09-01 00:00:00
abstract:BACKGROUND:Although Helicobacter pylori has been implicated in the pathogenesis of gastric mucosa associated lymphoid tissue (MALT) and MALT lymphoma, it is not known how it may trigger these lesions and whether there is an identifiable pre-neoplastic stage. AIMS:To investigate the relation between MALT, H pylori infe...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.38.6.837
更新日期:1996-06-01 00:00:00
abstract::In this study a perfusion technique has been used to investigate jejunal secretion in response to the dibasic amino acid L-arginine. L-arginine at 5, 15, and 40 mmol/l in isotonic saline solutions induced net intestinal secretion of water and Na+. The structurally similar dibasic amino acid L-lysine caused net absorpt...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.22.2.108
更新日期:1981-02-01 00:00:00
abstract:BACKGROUND:Tumour vascularisation is a determinant of the development of metastases. AIMS:To measure blood flow in normal stomach and gastric adenocarcinomas by laser Doppler flowmetry and correlate blood flow with vascularisation after immunohistochemical staining of resected specimens for CD31 and von Willebrand fac...
journal_title:Gut
pub_type: 临床试验,杂志文章
doi:10.1136/gut.45.3.341
更新日期:1999-09-01 00:00:00
abstract::Three cases are presented of lymphocytic colitis with chronic diarrhoea concurrent with longterm use of Cyclo 3 Fort, a phlebotonic drug used in France. The histological and immunopathological features of lymphocytic colitis are described. We show that lymphocytic colitis is drug induced, particularly in one patient w...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.35.3.426
更新日期:1994-03-01 00:00:00
abstract::A multidisciplinary panel of 18 physicians and 3 non-physicians from eight countries (Sweden, UK, Argentina, Australia, Italy, Finland, Norway and the USA) reviewed the literature on diagnosis and management of adult coeliac disease (CD). This paper presents the recommendations of the British Society of Gastroenterolo...
journal_title:Gut
pub_type: 杂志文章,实务指引
doi:10.1136/gutjnl-2013-306578
更新日期:2014-08-01 00:00:00
abstract::Pathogenic E coli have adhesive properties which are mirrored by an increase in their surface hydrophobicity. E coli isolated from patients with ulcerative colitis possess a mannose resistant adhesin similar to that found in pathogenic E coli. In this study 42 E coli isolates from patients with colitis have been compa...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.29.1.41
更新日期:1988-01-01 00:00:00
abstract::On three occasions, a 63 year old housewife with hyperthyroidism developed a reaction which included fever, pruritus, malaise, and, on one occasion, jaundice one to 17 days after taking carbimazole. Challenge with carbimazole was followed within 12 hours by abdominal pain, pruritus, and increased serum transaminase le...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.16.11.913
更新日期:1975-11-01 00:00:00
abstract:OBJECTIVE:Natural history of paediatric-onset ulcerative proctitis (UP) is poorly described. Our aim was to describe the phenotype and disease course of incident UP in a population-based study of paediatric-onset UC. PATIENTS AND METHODS:All patients with UC diagnosed <17 years from 1988 to 2004, and followed during >...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gutjnl-2016-311970
更新日期:2017-11-01 00:00:00
abstract::Two West Indian patients with Kveim-biopsy proven sarcoidosis developed chronic cholestatic liver disease, clinically and biochemically similar to primary biliary cirrhosis. Liver histology revealed multiple granulomas with reduction in bile ducts and, in one patient, progression to biliary cirrhosis. Portal hypertens...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.23.5.417
更新日期:1982-05-01 00:00:00
abstract::Probiotics are derived from traditional fermented foods, from beneficial commensals or from the environment. They act through diverse mechanisms affecting the composition or function of the commensal microbiota and by altering host epithelial and immunological responses. Certain probiotic interventions have shown prom...
journal_title:Gut
pub_type: 杂志文章,评审
doi:10.1136/gutjnl-2012-302504
更新日期:2013-05-01 00:00:00
abstract::Little is known about chronic constipation in infants, toddlers, and preschool children and longterm outcome after treatment. The symptoms of 174 children < or = 4 years of age, who were evaluated for chronic constipation, are reported in this study together with the long-term outcome in 90 of them. Initial symptoms w...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.34.10.1400
更新日期:1993-10-01 00:00:00
abstract::The possible relationship between enteroglucagon and cellular proliferation in a rat model of intestinal adaptation after suppression and stimulation of enteroglucagon by somatostatin and bombesin has been investigated. Forty eight rats were divided into three groups of 16 animals, each group being further sub-divided...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.26.1.89
更新日期:1985-01-01 00:00:00
abstract::Progress in the management of hepatocellular carcinoma (HCC) has been slow and has limited impact on outcome. Most patients with HCC have two diseases--chronic liver disease and HCC--and complex interactions between the two have major implications for diagnosis and prognosis as well as the management of HCC. The disea...
journal_title:Gut
pub_type: 杂志文章,评审
doi:10.1136/gut.51.4.459
更新日期:2002-10-01 00:00:00
abstract::The effect of octreotide, a synthetic analogue of somatostatin, on the modulation of the acetic acid model of experimental colitis was examined. Colitis was induced by intracolonic administration of 2 ml of 5% acetic acid. The inflammatory response elicited by the acetic acid resulted in increased colonic synthesis of...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.34.2.264
更新日期:1993-02-01 00:00:00
abstract::Although the development of endoscopic methods of treatment for biliary obstruction has proceeded rapidly in recent years, endoscopic retrograde cholangiopancreatographic (ERCP) services are patchily distributed. A recent survey by the British Society of Gastroenterology has shown that almost half the district general...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.31.10.1150
更新日期:1990-10-01 00:00:00
abstract:BACKGROUND:Somatostatin has been used to prevent pancreatitis after endoscopic retrograde cholangiopancreatography but its effect on acute non-biliary pancreatitis is still unclear. AIM:The purpose of this study was to evaluate the function of the sphincter of Oddi (SO) and the effect of somatostatin on patients with ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.49.6.843
更新日期:2001-12-01 00:00:00
abstract:BACKGROUND:Dyspepsia and irritable bowel syndrome are suitable conditions for assessment of quality of life. Their similarities justify the elaboration of a single specific questionnaire for the two conditions. AIMS:To examine the process leading to the validation of the psychometric properties of the functional diges...
journal_title:Gut
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1136/gut.44.4.527
更新日期:1999-04-01 00:00:00
abstract:BACKGROUND:Recently identified ABCG5/8 transporters are responsible in part for the different absorption rates of campesterol, sitosterol, and cholesterol. These transporters are also expressed in the liver and might regulate biliary sterol secretion. AIMS:This study was therefore conducted to determine the biliary se...
journal_title:Gut
pub_type: 临床试验,杂志文章
doi:10.1136/gut.51.6.860
更新日期:2002-12-01 00:00:00
abstract::Endoscopic sphincterotomy was undertaken in 186 patients with common bile duct stones and an intact gall bladder who were considered unfit for surgery. One hundred and seventy one patients had jaundice of whom 18 also had clinical cholangitis. The mean age of treated patients was 79.7 years (range 27-92) and only 13 w...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.30.8.1129
更新日期:1989-08-01 00:00:00
abstract::Glucosamine synthetase, the first enzyme in glycoprotein synthesis, has been measured in serial rectal biopsies in four patients with membranous colitis. In two of the patients the levels of the enzyme were very high initially and in the other two patients the enzyme levels rose to a peak above the normal range after ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.18.3.229
更新日期:1977-03-01 00:00:00