Serum zonulin and its diagnostic performance in non-coeliac gluten sensitivity.


OBJECTIVE:Non-coeliac gluten sensitivity (NCGS) is characterised by intestinal and extraintestinal symptoms related to the ingestion of gluten-containing foods, in the absence of coeliac disease (CD) and wheat allergy. No biomarkers are available to diagnose NCGS and the gold standard double-blind placebo-controlled gluten challenge is clinically impractical. The aim of our work was to investigate the role of serum zonulin as a diagnostic biomarker of NCGS and to develop a diagnostic algorithm. DESIGN:In a multicentre study, we enrolled 86 patients with either self-reported or double-blind confirmed NCGS, 59 patients with diarrhoea-predominant IBS (IBS-D), 15 patients with CD and 25 asymptomatic controls (AC). Zonulin serum levels were assessed and the associated diagnostic power calculated. Clinical and symptomatic data were recorded. The effect of diet on zonulin levels was evaluated in a subgroup of patients with NCGS. RESULTS:Compared with ACs, the NCGS, irrespective of modality of diagnosis, and patients with CD had significantly increased levels of zonulin, as did both NCGS and patients with CD compared with participants with IBS-D. Self-reported NCGS showed increased zonulin levels compared with double-blind confirmed and not-confirmed NCGS. Six-month wheat avoidance significantly reduced zonulin levels only in HLA-DQ2/8-positive participants with NCGS. The diagnostic accuracy of zonulin levels in distinguishing NCGS from IBS-D was 81%. After exclusion of CD, a diagnostic algorithm combining zonulin levels, symptoms and gender improved the accuracy to 89%. CONCLUSION:Zonulin can be considered a diagnostic biomarker in NCGS and combined with demographic and clinical data differentiates NCGS from IBS-D with high accuracy. Wheat withdrawal was associated with a reduction in zonulin levels only in NCGS carrying HLA genotype.






Barbaro MR,Cremon C,Morselli-Labate AM,Di Sabatino A,Giuffrida P,Corazza GR,Di Stefano M,Caio G,Latella G,Ciacci C,Fuschi D,Mastroroberto M,Bellacosa L,Stanghellini V,Volta U,Barbara G




Has Abstract


2020-11-01 00:00:00
















  • Clinical relevance of an unfused pancreatic duct system.

    abstract::In man, the main pancreatic duct is normally derived from ventral and dorsal embryological buds of the pancreas. In a minority of people, failure of fusion of the two buds results in separate drainage of the dorsal and ventral pancreas, so that the accessory duct provides the main drainage for the gland. Patients with...


    pub_type: 杂志文章


    authors: Mitchell CJ,Lintott DJ,Ruddell WS,Losowsky MS,Axon AT

    更新日期:1979-12-01 00:00:00

  • Clinical significance of SNORA42 as an oncogene and a prognostic biomarker in colorectal cancer.

    abstract:PURPOSE:Despite recent advances in colorectal cancer (CRC) treatment, the prognosis of patients suffering from this malignancy still remains substandard, and metastatic recurrence following curative surgery is the leading cause of mortality. Therefore, it is imperative to identify prognostic markers to predict the clin...


    pub_type: 杂志文章


    authors: Okugawa Y,Toiyama Y,Toden S,Mitoma H,Nagasaka T,Tanaka K,Inoue Y,Kusunoki M,Boland CR,Goel A

    更新日期:2017-01-01 00:00:00

  • Glucosamine synthetase activity of the colonic mucosa in membranous colitis.

    abstract::Glucosamine synthetase, the first enzyme in glycoprotein synthesis, has been measured in serial rectal biopsies in four patients with membranous colitis. In two of the patients the levels of the enzyme were very high initially and in the other two patients the enzyme levels rose to a peak above the normal range after ...


    pub_type: 杂志文章


    authors: Goodman MJ,Kent PW,Truelove SC

    更新日期:1977-03-01 00:00:00

  • International trends in oesophageal cancer survival by histological subtype between 1995 and 2014.

    abstract:INTRODUCTION:Survival from oesophageal cancer remains poor, even across high-income countries. Ongoing changes in the epidemiology of the disease highlight the need for survival assessments by its two main histological subtypes, adenocarcinoma (AC) and squamous cell carcinoma (SCC). METHODS:The ICBP SURVMARK-2 project...


    pub_type: 杂志文章


    authors: Morgan E,Soerjomataram I,Gavin AT,Rutherford MJ,Gatenby P,Bardot A,Ferlay J,Bucher O,De P,Engholm G,Jackson C,Kozie S,Little A,Møller B,Shack L,Tervonen H,Thursfield V,Vernon S,Walsh PM,Woods RR,Finley C,Merrett

    更新日期:2021-02-01 00:00:00

  • Rectal absorption of short chain fatty acids in the absence of chloride.

    abstract::The absorption of short chain fatty acids by the human rectum in the absence of chloride has been studied using a dialysis bag technique. Absorption rates of sodium, water, and short chain fatty acids were unchanged on replacing chloride with sulphate. Final concentrations of bicarbonate were 35.4 +/- 3.4 mmol/l in th...


    pub_type: 杂志文章


    authors: McNeil NI,Cummings JH,James WP

    更新日期:1979-05-01 00:00:00


    abstract::A clinical, biochemical, and pathological study is recorded of 50 patients in whom a diagnosis of idiopathic steatorrhea had been made and who had ;flat' jejunal biopsies. It is suggested that there is an underlying constitutional defect, not yet clearly defined, and that possibly secondary intestinal infection allows...


    pub_type: 杂志文章



    更新日期:1963-09-01 00:00:00

  • Timing of ileocolonic resection for symptomatic Crohn's disease--the patient's view.

    abstract::Eighty patients were asked if they would have preferred their ileocolonic resection and anastomosis for Crohn's disease, to have been carried out sooner, later or at the same time as it was done. Seventy of the patients replied (88%). No patient would have preferred their operation to have been later, while 74% though...


    pub_type: 杂志文章


    authors: Scott NA,Hughes LE

    更新日期:1994-05-01 00:00:00

  • JAK selectivity for inflammatory bowel disease treatment: does it clinically matter?

    abstract::The two major forms of inflammatory bowel disease (IBD), encompassing Crohn's disease (CD) and ulcerative colitis (UC), are chronic immune-mediated conditions characterised by an increased production of pro-inflammatory cytokines that act as critical drivers of intestinal inflammation. Anti-cytokine therapy has been s...


    pub_type: 杂志文章,评审


    authors: Danese S,Argollo M,Le Berre C,Peyrin-Biroulet L

    更新日期:2019-10-01 00:00:00

  • Nippostrongylus brasiliensis infection in the rat: effect of iron and protein deficiency on the anthelmintic efficacy of mebendazole, pyrantel, piperazine, and levamisole.

    abstract::The benzimidazole anthelmintics mebendazole and fenbendazole have been shown to be much less effective against Nippostrongylus brasiliensis infections in the rat on a combined iron and protein deficient diet. In the present experiments it was shown that the anthelmintic efficacy of mebendazole was significantly impair...


    pub_type: 杂志文章


    authors: Duncombe VM,Bolin TD,Davis AE,Fagan MR,Kelly JD

    更新日期:1979-04-01 00:00:00

  • Significance of systemic endotoxaemia in inflammatory bowel disease.

    abstract::Quantitative and qualitative disturbances in faecal flora suggest a role for enteric bacteria and their products in the pathogenesis of inflammatory bowel disease (IBD). This study investigated the hypothesis that systemically circulating endotoxins are of pathogenic significance in IBD by measuring antibody, cytokine...


    pub_type: 杂志文章


    authors: Gardiner KR,Halliday MI,Barclay GR,Milne L,Brown D,Stephens S,Maxwell RJ,Rowlands BJ

    更新日期:1995-06-01 00:00:00

  • Cannabinoids and the gastrointestinal tract.

    abstract::The enteric nervous system of several species, including the mouse, rat, guinea pig and humans, contains cannabinoid CB1 receptors that depress gastrointestinal motility, mainly by inhibiting ongoing contractile transmitter release. Signs of this depressant effect are, in the whole organism, delayed gastric emptying a...


    pub_type: 杂志文章,评审


    authors: Pertwee RG

    更新日期:2001-06-01 00:00:00

  • Randomised, double blind, placebo controlled trial of intravenous antioxidant (n-acetylcysteine, selenium, vitamin C) therapy in severe acute pancreatitis.

    abstract:BACKGROUND:Based on equivocal clinical data, intravenous antioxidant therapy has been used for the treatment of severe acute pancreatitis. To date there is no randomised comparison of this therapy in severe acute pancreatitis. METHODS:We conducted a randomised, double blind, placebo controlled trial of intravenous ant...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Siriwardena AK,Mason JM,Balachandra S,Bagul A,Galloway S,Formela L,Hardman JG,Jamdar S

    更新日期:2007-10-01 00:00:00

  • Endotoxin, cytokines, and endotoxin binding proteins in obstructive jaundice and after preoperative biliary drainage.

    abstract:BACKGROUND:Obstructive jaundice is associated with postoperative complications related to increased endotoxaemia and the inflammatory response. In animals obstructive jaundice is associated with endotoxaemia and cytokine induction, which are reversed by internal biliary drainage. AIMS:To study endotoxaemia and the sub...


    pub_type: 杂志文章


    authors: Kimmings AN,van Deventer SJ,Obertop H,Rauws EA,Huibregtse K,Gouma DJ

    更新日期:2000-05-01 00:00:00

  • Increased interleukin 8 expression in the colon mucosa of patients with inflammatory bowel disease.

    abstract::To test whether there is a difference in the expression of interleukin 8 (IL8) between Crohn's disease and ulcerative colitis and to determine the main site of its synthesis this study analysed IL8 in mucosal biopsy specimens of patients with Crohn's disease and ulcerative colitis by enzyme linked immunosorbent assay ...


    pub_type: 杂志文章


    authors: Daig R,Andus T,Aschenbrenner E,Falk W,Schölmerich J,Gross V

    更新日期:1996-02-01 00:00:00

  • Effectiveness of AFP and ultrasound tests on hepatocellular carcinoma mortality in HCV-infected patients in the USA.

    abstract:BACKGROUND AND AIMS:The effectiveness of surveillance for hepatocellular carcinoma (HCC) in the USA is largely unknown. The objective of this study was to evaluate the effectiveness of HCC surveillance in a national Veterans Administration (VA) practice setting, using the national VA hepatitis C virus (HCV) Clinical Ca...


    pub_type: 杂志文章


    authors: El-Serag HB,Kramer JR,Chen GJ,Duan Z,Richardson PA,Davila JA

    更新日期:2011-07-01 00:00:00

  • RSPO2 gene rearrangement: a powerful driver of β-catenin activation in liver tumours.

    abstract:OBJECTIVE:We aimed at the identification of genetic alterations that may functionally substitute for CTNNB1 mutation in ß-catenin-activated hepatocellular adenomas (HCAs) and hepatocellular carcinoma (HCC). DESIGN:Large cohorts of HCA (n=185) and HCC (n=468) were classified using immunohistochemistry. The mutational s...


    pub_type: 杂志文章


    authors: Longerich T,Endris V,Neumann O,Rempel E,Kirchner M,Abadi Z,Uhrig S,Kriegsmann M,Weiss KH,Breuhahn K,Mehrabi A,Weber TF,Wilkens L,Straub BK,Rosenwald A,Schulze F,Brors B,Froehling S,Pellegrino R,Budczies J,Schirmac

    更新日期:2019-07-01 00:00:00

  • Hepatitis C virus: epidemiology and genotypes in the north east of England.

    abstract::The epidemiology of hepatitis C virus (HCV) infection was studied in an English teaching hospital over an 18 month period. A total of 104 HCV antibody positive patients were referred for further investigation. They were divided into those diagnosed through screening (blood donors and intravenous drug abusers) and thos...


    pub_type: 杂志文章


    authors: Watson JP,Brind AM,Chapman CE,Bates CL,Gould FK,Johnson SJ,Burt AD,Ferguson J,Simmonds P,Bassendine MF

    更新日期:1996-02-01 00:00:00

  • Inhibitory effect of a cholecystokinin antagonist on the proliferative response of the pancreas to pancreatobiliary diversion.

    abstract::Since pancreatobiliary diversion probably stimulates pancreatic growth by increasing cholecystokinin secretion, the effect of the cholecystokinin antagonist CR-1409 on this adaptive response was tested. Male Wistar rats (n = 108) weighing 220-250g were randomised to receive either pancreatobiliary diversion (n = 60) o...


    pub_type: 杂志文章


    authors: Watanapa P,Efa EF,Beardshall K,Calam J,Sarraf CE,Alison MR,Williamson RC

    更新日期:1991-09-01 00:00:00

  • Claudin-4-targeted optical imaging detects pancreatic cancer and its precursor lesions.

    abstract:OBJECTIVES:Novel imaging methods based on specific molecular targets to detect both established neoplasms and their precursor lesions are highly desirable in cancer medicine. Previously, we identified claudin-4, an integral constituent of tight junctions, as highly expressed in various gastrointestinal tumours includin...


    pub_type: 杂志文章


    authors: Neesse A,Hahnenkamp A,Griesmann H,Buchholz M,Hahn SA,Maghnouj A,Fendrich V,Ring J,Sipos B,Tuveson DA,Bremer C,Gress TM,Michl P

    更新日期:2013-07-01 00:00:00

  • Rôle of calcitonin in diarrhoea associated with medullary carcinoma of the thyroid.

    abstract::Severe watery diarrhoea in a patient with medullary thryoid carcinoma and high calcitonin levels was associated with increased distal ileal flow. Plasma from the patient inhibited small intestinal fluid absorption in dogs without affecting mucosal cyclic AMP levels. When calcitonin was removed from the plasma, the eff...


    pub_type: 杂志文章


    authors: Cox TM,Fagan EA,Hillyard CJ,Allison DJ,Chadwick VS

    更新日期:1979-07-01 00:00:00

  • Mucosal sulfhydryl compounds evaluation by in vivo electron spin resonance spectroscopy in mice with experimental colitis.

    abstract:BACKGROUND:Sulfhydryl (SH) compounds are essential in maintaining mucosal integrity in the gastrointestinal tract. A decrease in colonic mucosal SH compounds affects the redox status of the mucosa, resulting in vulnerability to further attacks. Therefore, there is a strong need for in vivo evaluation of SH compounds in...


    pub_type: 杂志文章


    authors: Togashi H,Oikawa K,Adachi T,Sugahara K,Ito J,Takeda T,Watanabe H,Saito K,Saito T,Fukui T,Takeda H,Ohya H,Kawata S

    更新日期:2003-09-01 00:00:00

  • Long-term instability of the intestinal microbiome is associated with metabolic liver disease, low microbiota diversity, diabetes mellitus and impaired exocrine pancreatic function.

    abstract:OBJECTIVE:The intestinal microbiome affects the prevalence and pathophysiology of a variety of diseases ranging from inflammation to cancer. A reduced taxonomic or functional diversity of the microbiome was often observed in association with poorer health outcomes or disease in general. Conversely, factors or manifest ...


    pub_type: 杂志文章


    authors: Frost F,Kacprowski T,Rühlemann M,Pietzner M,Bang C,Franke A,Nauck M,Völker U,Völzke H,Dörr M,Baumbach J,Sendler M,Schulz C,Mayerle J,Weiss FU,Homuth G,Lerch MM

    更新日期:2020-11-09 00:00:00

  • Abnormalities of B-cell activation and immunoregulation in patients with Crohn's disease.

    abstract::We have studied B-lymphocyte function in 39 patients with Crohn's disease and 35 normal individuals using a reverse haemolytic plaque assay as the effector system. Ten patients had active Crohn's disease, the others being in an inactive state of the disease. Compared with normal individuals, the Crohn's disease patien...


    pub_type: 杂志文章


    authors: Sieber G,Herrmann F,Zeitz M,Teichmann H,Rühl H

    更新日期:1984-11-01 00:00:00

  • Benign lymphangioma of the jejunal mesentery: an unusual cause of small bowel obstruction.

    abstract::Lymphangiomas are rare tumours of lymphatic vessels, most commonly found in children. We present the unusual case of small bowel obstruction caused by benign lymphangioma in a middle aged woman. ...


    pub_type: 杂志文章


    authors: Campbell WJ,Irwin ST,Biggart JD

    更新日期:1991-12-01 00:00:00

  • Laparoscopic biliary and gastric bypass: a useful adjunct in the treatment of carcinoma of the pancreas.

    abstract::Over 90% of patients with inoperable carcinoma of the pancreas are successfully palliated by endoscopic retrograde cholangiopancreatography and stent insertion. Treatment of the residual 10% of patients often entails a laparotomy, which is difficult to justify when median survival of these patients is only 150 days. L...


    pub_type: 杂志文章


    authors: Rhodes M,Nathanson L,Fielding G

    更新日期:1995-05-01 00:00:00

  • Evaluation of indium scintigraphy in patients with active inflammatory bowel disease.

    abstract::Recent reports have indicated a possible role for 111Indium leucocyte scintigraphy in the management of patients with acute inflammatory bowel disease. Our experience with this technique in 15 patients (nine with ulcerative colitis, six with Crohn's disease) is described. Perfect agreement was not obtained between sci...


    pub_type: 杂志文章


    authors: Buxton-Thomas MS,Dickinson RJ,Maltby P,Hunter JO,Wraight EP

    更新日期:1984-12-01 00:00:00

  • Potentiation of TRPV4 signalling by histamine and serotonin: an important mechanism for visceral hypersensitivity.

    abstract:BACKGROUND:Although evidence points to a role for histamine and serotonin in visceral hypersensitivity, activation of calcium channels such as transient receptor potential vanilloid 4 (TRPV4) also causes visceral hypersensitivity. We hypothesised that TRPV4 is important for the generation of hypersensitivity, mediating...


    pub_type: 杂志文章


    authors: Cenac N,Altier C,Motta JP,d'Aldebert E,Galeano S,Zamponi GW,Vergnolle N

    更新日期:2010-04-01 00:00:00

  • Anti-inflammatory role of sympathetic nerves in chronic intestinal inflammation.

    abstract:BACKGROUND:Substance P (SP) is a pro-inflammatory neuropeptide in colitis, whereas sympathetic neurotransmitters are anti-inflammatory at high concentrations. AIM AND METHODS:In all layers of the colon, nerve fibre densities of SP(+) and sympathetic nerve fibres were investigated (22 Crohn's disease, six diverticuliti...


    pub_type: 杂志文章


    authors: Straub RH,Grum F,Strauch U,Capellino S,Bataille F,Bleich A,Falk W,Schölmerich J,Obermeier F

    更新日期:2008-07-01 00:00:00

  • Long-term performance of colorectal cancerscreening programmes based on the faecal immunochemical test.

    abstract:BACKGROUND:The long-term performance of colorectal cancer (CRC) screening programmes based on a 2-year faecal immunochemical test (FIT) is still unclear. METHODS:In a sample of 50 to 69-year-olds repeatedly screened with the FIT (OC-Hemodia latex agglutination test; cut-off: 20 µg haemoglobin/g faeces), we examined: (...


    pub_type: 杂志文章


    authors: Zorzi M,Hassan C,Capodaglio G,Fedato C,Montaguti A,Turrin A,Rosano A,Monetti D,Stocco C,Baracco S,Russo F,Repici A,Rugge M

    更新日期:2018-12-01 00:00:00

  • Clq metabolism in ulcerative colitis and Crohn's disease.

    abstract::The metabolism of pure radioiodine labelled Clq has been observed in five patients with ulcerative colitis, five patients with Crohn's disease, and in five control subjects. Both the fractional catabolic rate and the synthesis rate of Clq were increased in the five patients with Crohn's disease and in four of the five...


    pub_type: 杂志文章


    authors: Potter BJ,Hodgson HJ,Mee AS,Jewell DP

    更新日期:1979-11-01 00:00:00