Serum zonulin and its diagnostic performance in non-coeliac gluten sensitivity.


OBJECTIVE:Non-coeliac gluten sensitivity (NCGS) is characterised by intestinal and extraintestinal symptoms related to the ingestion of gluten-containing foods, in the absence of coeliac disease (CD) and wheat allergy. No biomarkers are available to diagnose NCGS and the gold standard double-blind placebo-controlled gluten challenge is clinically impractical. The aim of our work was to investigate the role of serum zonulin as a diagnostic biomarker of NCGS and to develop a diagnostic algorithm. DESIGN:In a multicentre study, we enrolled 86 patients with either self-reported or double-blind confirmed NCGS, 59 patients with diarrhoea-predominant IBS (IBS-D), 15 patients with CD and 25 asymptomatic controls (AC). Zonulin serum levels were assessed and the associated diagnostic power calculated. Clinical and symptomatic data were recorded. The effect of diet on zonulin levels was evaluated in a subgroup of patients with NCGS. RESULTS:Compared with ACs, the NCGS, irrespective of modality of diagnosis, and patients with CD had significantly increased levels of zonulin, as did both NCGS and patients with CD compared with participants with IBS-D. Self-reported NCGS showed increased zonulin levels compared with double-blind confirmed and not-confirmed NCGS. Six-month wheat avoidance significantly reduced zonulin levels only in HLA-DQ2/8-positive participants with NCGS. The diagnostic accuracy of zonulin levels in distinguishing NCGS from IBS-D was 81%. After exclusion of CD, a diagnostic algorithm combining zonulin levels, symptoms and gender improved the accuracy to 89%. CONCLUSION:Zonulin can be considered a diagnostic biomarker in NCGS and combined with demographic and clinical data differentiates NCGS from IBS-D with high accuracy. Wheat withdrawal was associated with a reduction in zonulin levels only in NCGS carrying HLA genotype.






Barbaro MR,Cremon C,Morselli-Labate AM,Di Sabatino A,Giuffrida P,Corazza GR,Di Stefano M,Caio G,Latella G,Ciacci C,Fuschi D,Mastroroberto M,Bellacosa L,Stanghellini V,Volta U,Barbara G




Has Abstract


2020-11-01 00:00:00
















  • Detection of subclinical autonomic neuropathy in constipated patients using a sweat test.

    abstract::Chronic idiopathic constipation may be the result of an autonomic neuropathy. This hypothesis was tested in 23 constipated patients and 17 age matched controls, using the acetylcholine sweat spot test devised to test autonomic integrity in diabetes. Acetylcholine (0.01%) was injected in the dorsum of the foot painted ...


    pub_type: 杂志文章


    authors: Altomare D,Pilot MA,Scott M,Williams N,Rubino M,Ilincic L,Waldron D

    更新日期:1992-11-01 00:00:00

  • Use of an inert marker (phenol red) to improve accuracy in gastric secretion studies.

    abstract::Gastric secretion from 24 subjects was collected by continuous nasogastric aspiration during the plateau of secretion evoked by intravenous histamine diphosphate, 0.01 mg/kg/hr. Simultaneously, phenol red was instilled at a constant rate into the proximal part of the stomach. Concentrations of phenol red in 15-minute ...


    pub_type: 杂志文章


    authors: Hobsley M,Silen W

    更新日期:1969-10-01 00:00:00

  • In healthy volunteers, immunohistochemistry supports squamous to columnar metaplasia as mechanism of expansion of cardia, aggravated by central obesity.

    abstract:INTRODUCTION:Recently, we showed that the length of cardiac mucosa in healthy volunteers correlated with age and obesity. We have now examined the immunohistological characteristics of this expanded cardia to determine whether it may be due to columnar metaplasia of the distal oesophagus. METHODS:We used the squamocol...


    pub_type: 杂志文章


    authors: Derakhshan MH,Robertson EV,Yeh Lee Y,Harvey T,Ferrier RK,Wirz AA,Orange C,Ballantyne SA,Hanvey SL,Going JJ,McColl KE

    更新日期:2015-11-01 00:00:00

  • Batf-dependent Th17 cells critically regulate IL-23 driven colitis-associated colon cancer.

    abstract:OBJECTIVES:IBDs have an increased risk for development of colorectal cancer (CRC). Here, we aimed at the characterisation of the functional role of Th17-associated transcription factors in sporadic and colitis-associated colon cancer in vivo. DESIGN:We used mice deficient or transgenic for the activating protein 1 fam...


    pub_type: 杂志文章


    authors: Punkenburg E,Vogler T,Büttner M,Amann K,Waldner M,Atreya R,Abendroth B,Mudter J,Merkel S,Gallmeier E,Rose-John S,Neurath MF,Hildner K

    更新日期:2016-07-01 00:00:00

  • Opportunistic infections in patients with inflammatory bowel disease: prevention and diagnosis.

    abstract::Because of the increasing use of immunosuppressive and biological drugs, the occurrence of opportunistic infections has become a key safety issue for patients with inflammatory bowel disease (IBD). Consequently, improvement of healthcare workers' knowledge of this domain is urgent. In this review, the preventive measu...


    pub_type: 杂志文章,评审


    authors: Viget N,Vernier-Massouille G,Salmon-Ceron D,Yazdanpanah Y,Colombel JF

    更新日期:2008-04-01 00:00:00

  • Consequences of partial and subtotal colectomy in the rat.

    abstract::Rats were subjects to right hemicolectomy (including removal of the caecum), left hemicolectomy or subtotal colectomy. Body weight resumed and maintained a rate of increase very similar to that in control rats. After hemicolectomy, food intake showed no change. Faecal weight increased by about one-third after right he...


    pub_type: 杂志文章


    authors: Masesa PC,Forrester JM

    更新日期:1977-01-01 00:00:00

  • Genomic ERBB2/ERBB3 mutations promote PD-L1-mediated immune escape in gallbladder cancer: a whole-exome sequencing analysis.

    abstract:OBJECTIVES:Patients with gallbladder carcinoma (GBC) lack effective treatment methods largely due to the inadequacy of both molecular characterisation and potential therapeutic targets. We previously uncovered a spectrum of genomic alterations and identified recurrent mutations in the ErbB pathway in GBC. Here, we aime...


    pub_type: 杂志文章


    authors: Li M,Liu F,Zhang F,Zhou W,Jiang X,Yang Y,Qu K,Wang Y,Ma Q,Wang T,Bai L,Wang Z,Song X,Zhu Y,Yuan R,Gao Y,Liu Y,Jin Y,Li H,Xiang S,Ye Y,Zhang Y,Jiang L,Hu Y,Hao Y,Lu W,Chen S,Gu J,Zhou J,Gong W,Z

    更新日期:2019-06-01 00:00:00

  • Mucus degradation by pepsin: comparison of mucolytic activity of human pepsin 1 and pepsin 3: implications in peptic ulceration.

    abstract::The ability to digest mucus, mucolytic activity of isolated pepsins and samples of human gastric juice has been assayed by measuring the fall in viscosity when incubated with purified pig gastric mucus glycoprotein. Pure human pepsin 1, the peptic ulcer associated pepsin, digested gastric mucus glycoprotein at a faste...


    pub_type: 杂志文章


    authors: Pearson JP,Ward R,Allen A,Roberts NB,Taylor WH

    更新日期:1986-03-01 00:00:00

  • Micrometastases: marker of metastatic potential or evidence of residual disease?

    abstract:BACKGROUND:Micrometastases within bone marrow have been shown to indicate a poor prognosis in patients with epithelial tumours. However, the degree to which micrometastases represent true residual disease or cell shedding and metastatic potential, is unclear. AIM:To explore whether micrometastases represent residual d...


    pub_type: 杂志文章


    authors: O'Sullivan GC,Collins JK,Kelly J,Morgan J,Madden M,Shanahan F

    更新日期:1997-04-01 00:00:00

  • Trials and tribulations with artificial liver support.

    abstract::This paper reviews the experience gained over the past few years in two techniques of liver support, charcoal haemoperfusion and polyacrylonitrile membrane haemodialysis. Problems with charcoal haemoperfusion have centred around platelet aggregation and hypertension, and new carbon preparations with a variety of diffe...


    pub_type: 杂志文章,评审


    authors: Williams R

    更新日期:1978-06-01 00:00:00

  • Malignant histiocytosis of the intestine: the early histological lesion.

    abstract::A histological study of peroral jejunal biopsies and resection specimens from patients with malignant histiocytosis of the intestine (MHI) has revealed characteristic lesions consisting of intramucosal histiocytic aggregations which invade surface and crypt epithelium. These lesions are found in 'uninvolved' jejunal m...


    pub_type: 杂志文章


    authors: Isaacson P

    更新日期:1980-05-01 00:00:00

  • Eradication of Helicobacter pylori with clarithromycin and omeprazole.

    abstract::Clarithromycin, a new and well tolerated, acid stable macrolide antibiotic, has a similar antimicrobial spectrum to erythromycin but a better in vitro MIC90 (0.03 microgram/l-1) against Helicobacter pylori (H pylori). This study aimed at determining the eradication rate using clarithromycin 500 mg thrice daily and ome...


    pub_type: 杂志文章


    authors: Logan RP,Gummett PA,Schaufelberger HD,Greaves RR,Mendelson GM,Walker MM,Thomas PH,Baron JH,Misiewicz JJ

    更新日期:1994-03-01 00:00:00

  • Hydrophobic adhesin of E coli in ulcerative colitis.

    abstract::Pathogenic E coli have adhesive properties which are mirrored by an increase in their surface hydrophobicity. E coli isolated from patients with ulcerative colitis possess a mannose resistant adhesin similar to that found in pathogenic E coli. In this study 42 E coli isolates from patients with colitis have been compa...


    pub_type: 杂志文章


    authors: Burke DA,Axon AT

    更新日期:1988-01-01 00:00:00

  • Global DNA hypomethylation occurs in the early stages of intestinal type gastric carcinoma.

    abstract:BACKGROUND:Global DNA hypomethylation has been found in the premalignant stages of some neoplasms and has been implicated as an important factor for tumour progression. AIMS:The aim of this study was to evaluate whether DNA hypomethylation occurs during the process of gastric carcinogenesis. METHODS:Gastric specimens...


    pub_type: 杂志文章


    authors: Cravo M,Pinto R,Fidalgo P,Chaves P,Glória L,Nobre-Leitão C,Costa Mira F

    更新日期:1996-09-01 00:00:00

  • Aminoacid constitution of two gastrins isolated from Zollinger-Ellison tumour tissue.

    abstract::Two peptides which are potent stimulants of gastric acid secretion are isolated from Zollinger-Ellison tumour tissue. They have aminoacid constitutions identical with those of human gastrin types I and II as isolated from human antral mucosa, and they are present in similar proportions. Their electrophoretic and chrom...


    pub_type: 杂志文章


    authors: Gregory RA,Tracy HJ,Agarwal KL,Grossman MI

    更新日期:1969-08-01 00:00:00

  • Clinical and laboratory study of postvagotomy diarrhoea.

    abstract::Thirty-two patients with diarrhoea, on average four years following truncal vagotomy and drainage, were studied. A comparison was made with 24 patients without postvagotomy diarrhoea. The incidence of bacterial colonization of the upper small intestine was no different in the two groups, though patients with a gastroe...


    pub_type: 杂志文章


    authors: Browning GG,Buchan KA,Mackay C

    更新日期:1974-08-01 00:00:00

  • Comparison of the clinical prediction model PREMM(1,2,6) and molecular testing for the systematic identification of Lynch syndrome in colorectal cancer.

    abstract:BACKGROUND:Lynch syndrome is caused by germline mismatch repair (MMR) gene mutations. The PREMM(1,2,6) model predicts the likelihood of a MMR gene mutation based on personal and family cancer history. OBJECTIVE:To compare strategies using PREMM(1,2,6) and tumour testing (microsatellite instability (MSI) and/or immunoh...


    pub_type: 杂志文章,多中心研究


    authors: Kastrinos F,Steyerberg EW,Balmaña J,Mercado R,Gallinger S,Haile R,Casey G,Hopper JL,LeMarchand L,Lindor NM,Newcomb PA,Thibodeau SN,Syngal S,Colon Cancer Family Registry.

    更新日期:2013-02-01 00:00:00

  • Elevated plasma osteopontin associated with gastric cancer development, invasion and survival.

    abstract:OBJECTIVE:Osteopontin (OPN) has been found to be valuable in diagnosis and predicting the prognosis of a variety of malignancies. The aims of the present study are to evaluate the usefulness of plasma OPN level for predicting gastric cancer development, invasion and survival. PATIENTS AND METHODS:One hundred and thirt...


    pub_type: 杂志文章


    authors: Wu CY,Wu MS,Chiang EP,Wu CC,Chen YJ,Chen CJ,Chi NH,Chen GH,Lin JT

    更新日期:2007-06-01 00:00:00

  • B-cell clonality and infection with Helicobacter pylori: implications for development of gastric lymphoma.

    abstract:BACKGROUND:Although Helicobacter pylori has been implicated in the pathogenesis of gastric mucosa associated lymphoid tissue (MALT) and MALT lymphoma, it is not known how it may trigger these lesions and whether there is an identifiable pre-neoplastic stage. AIMS:To investigate the relation between MALT, H pylori infe...


    pub_type: 杂志文章


    authors: Sorrentino D,Ferraccioli GF,DeVita S,Avellini C,Beltrami CA,Labombarda A,Bernardis V,De Biase F,Trevisi A,Pivetta B,Boiocchi M,Bartoli E

    更新日期:1996-06-01 00:00:00

  • GATA6 regulates EMT and tumour dissemination, and is a marker of response to adjuvant chemotherapy in pancreatic cancer.

    abstract:BACKGROUND AND AIMS:The role of GATA factors in cancer has gained increasing attention recently, but the function of GATA6 in pancreatic ductal adenocarcinoma (PDAC) is controversial. GATA6 is amplified in a subset of tumours and was proposed to be oncogenic, but high GATA6 levels are found in well-differentiated tumou...


    pub_type: 杂志文章


    authors: Martinelli P,Carrillo-de Santa Pau E,Cox T,Sainz B Jr,Dusetti N,Greenhalf W,Rinaldi L,Costello E,Ghaneh P,Malats N,Büchler M,Pajic M,Biankin AV,Iovanna J,Neoptolemos J,Real FX

    更新日期:2017-09-01 00:00:00

  • Insulin-like growth factor I receptor blockade enhances chemotherapy and radiation responses and inhibits tumour growth in human gastric cancer xenografts.

    abstract:BACKGROUND AND AIMS:Insulin-like growth factor (IGF) I receptor (IGF-Ir) signalling is required for carcinogenicity and proliferation of many tumours but this pathway has not been studied in detail in gastric cancer. We have previously shown successful therapy for colorectal, pancreatic, and lung cancer using recombina...


    pub_type: 杂志文章


    authors: Min Y,Adachi Y,Yamamoto H,Imsumran A,Arimura Y,Endo T,Hinoda Y,Lee CT,Nadaf S,Carbone DP,Imai K

    更新日期:2005-05-01 00:00:00

  • Gastric motility and emptying in normal and post-vagotomy subjects.

    abstract::The effects of proximal gastric vagotomy (PGV) and vagotomy with pyloroplasty (V and P) on gastric motility were studied using a solid meal labelled with a radiopharmaceutical agent. In having on-line computer facilities it was possible not only to record the rate of emptying but also to analyse the relative roles of ...


    pub_type: 杂志文章


    authors: Sheiner HJ,Quinlan MF,Thompson IJ

    更新日期:1980-09-01 00:00:00

  • Evidence of a systemic phenomenon for oxidative stress in cholestatic liver disease.

    abstract:BACKGROUND:There is considerable evidence indicating that the severity of hepatic damage in individuals with cholestatic liver disease is causally associated with the extent of intrahepatic oxidative stress. Increased levels or accelerated generation of reactive oxygen species and toxic degradative products of lipid pe...


    pub_type: 杂志文章


    authors: Ljubuncic P,Tanne Z,Bomzon A

    更新日期:2000-11-01 00:00:00

  • Pancreatic islet cell tumour with watery diarrhoea and hypokalaemia.

    abstract::A further patient with watery diarrhoea and hypokalaemia in association with a non-beta islet cell tumour of the pancreas is reported. The mechanism by which fluid and electrolyte losses are produced is discussed and cases previously reported are reviewed. ...


    pub_type: 杂志文章


    authors: Stoker DJ,Wynn V

    更新日期:1970-11-01 00:00:00

  • Yellowish lesions in the oesophagus.

    abstract::CLINICAL PRESENTATION: A 51-year-old man, who had no previous disease, underwent a screening oesophagogastroduodenoscopy, which revealed multiple yellowish lesions in the middle thoracic oesophagus. All lesions were ≤3 mm in size and slightly elevated. Each lesion showed one or two white protrusions on the surface (fi...


    pub_type: 杂志文章


    authors: Hamada K,Yamasaki Y,Kubota JI,Okada H

    更新日期:2019-01-01 00:00:00

  • Increased cell membrane arachidonic acid in experimental colorectal tumours.

    abstract::Tumour cell membrane fatty acid composition was investigated using an animal model of colorectal carcinogenesis. Eighty six male Wistar rats were fed experimental diets containing either 5% saturated fat or 20% saturated fat. Colorectal tumours were induced by intraperitoneal injection of azoxymethane, and control rat...


    pub_type: 杂志文章


    authors: Nicholson ML,Neoptolemos JP,Clayton HA,Talbot IC,Bell PR

    更新日期:1991-04-01 00:00:00

  • Refractory pouchitis: does it reflect underlying Crohn's disease?

    abstract::Typical 'pouchitis' is a well recognised complication of ileal pouches in ulcerative colitis. Infrequently, a refractory pouchitis (RP) presents with certain clinical, endoscopic, and pathological features resembling Crohn's disease and is often ascribed to misdiagnosis of the initial colitis. To test that hypothesis ...


    pub_type: 杂志文章


    authors: Subramani K,Harpaz N,Bilotta J,Bodian C,Rubin PH,Janowitz HD,Gelernt IM,Sachar DB

    更新日期:1993-11-01 00:00:00

  • Potential difference across the normal and the abnormal gastric mucosa in man.

    abstract::The mucosal potential difference in the body of the stomach was measured in 18 patients with gastric ulcers, five with gastritis, and three with pernicious anaemia. The results were compared with those of 12 normal volunteers. Significantly lower than normal levels were found in the groups of patients with gastric ulc...


    pub_type: 杂志文章


    authors: Hossenbocus A,Fitzpatrick P,Colin-Jones DG

    更新日期:1976-12-01 00:00:00

  • Role of TLR4 in the gut-brain axis in Parkinson's disease: a translational study from men to mice.

    abstract:OBJECTIVE:Recent evidence suggesting an important role of gut-derived inflammation in brain disorders has opened up new directions to explore the possible role of the gut-brain axis in neurodegenerative diseases. Given the prominence of dysbiosis and colonic dysfunction in patients with Parkinson's disease (PD), we pro...


    pub_type: 杂志文章


    authors: Perez-Pardo P,Dodiya HB,Engen PA,Forsyth CB,Huschens AM,Shaikh M,Voigt RM,Naqib A,Green SJ,Kordower JH,Shannon KM,Garssen J,Kraneveld AD,Keshavarzian A

    更新日期:2019-05-01 00:00:00

  • Management of bleeding oesophageal varices by draining lymph from the thoracic duct.

    abstract::The thoracic duct was cannulated in six patients who were actively bleeding from varices and had not responded to conservative treatment but draining lymph from the thoracic duct did not control bleeding in five patients. There was no drop in intrasplenic pulp pressure following this procedure when recorded in three o...


    pub_type: 杂志文章


    authors: Datta DV,Singh SA,Patra BS,Saini VK,Chhuttani PN

    更新日期:1971-01-01 00:00:00