Abstract：
:Nerve guide implants approved for human application in the peripheral nervous system generally fail to bridge lesion gaps longer than 2 cm and cannot match the clinical performance of autologous nerve transplants. Since current synthetic implants are simply hollow tubes, we aim to recreate the native microarchitecture of nerves inside the tubular implants. Most importantly, in the regenerating nerve, dedifferentiated Schwann cells align to form thousands of long glial strands, which act as guiding structures for the regrowing axons. In order to artificially induce the formation of Schwann cell strands, 28 μm thick, endless poly-p-dioxanone filaments (PDO) were synthesized with longitudinal grooves. A polycationic coating on the PDO filaments rendered the polymer surface cell-permissive and induced the growth of highly oriented Schwann cells with polarized expression of N-cadherin at cell-cell contact sites. In vitro cell proliferation on three-dimensional PDO filaments was significantly increased in comparison to planar PDO substrates. Time lapse video recordings revealed high Schwann cell motility, which is advantageous for the repopulation of cell-free implants after implantation. In a pilot study we employed a novel microsurgical technique in vivo. All axon fascicles were selectively dissected from sciatic rat nerves, and the remaining epineural tube was filled with hundreds of PDO filaments. Histological analysis 6 weeks postoperatively showed no fibrosis or encapsulation but instead longitudinal cell alignment and axonal regrowth. The data suggest that the addition of microstructured PDO filaments to the lumen of synthetic tubular implants might significantly improve performance.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Nichterwitz S,Hoffmann N,Hajosch R,Oberhoffner S,Schlosshauer Bdoi
10.1016/j.neulet.2010.08.028subject
Has Abstractpub_date
2010-10-29 00:00:00pages
118-22issue
2eissn
0304-3940issn
1872-7972pii
S0304-3940(10)01074-8journal_volume
484pub_type
杂志文章abstract：:The spontaneous electrical activity of single neurons of the midbrain dorsal raphe nucleus in the rat was recorded in vitro in 400 mum thick slices. The majority of raphe neurons exhibited the slow and regular discharge pattern that characterizes the activity of raphe neurons when recorded in vivo. The viability of th...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(76)90014-8
更新日期：1976-06-01 00:00:00
abstract：:Vascular endothelial growth factor (VEGF) is a major regulator of angiogenesis and blood vessel function. Recent evidence indicates that VEGF facilitates memory and learning through stimulating angiogenesis and neurogenesis in the rat hippocampal dendate gyrus. Abnormal regulation of VEGF expression has been reported ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2006.03.020
更新日期：2006-06-19 00:00:00
abstract：:A tight coupling between lexico-semantic access and motor control has been established on the basis of neuropsychological, neurophysiological, and behavioral evidence. For example, sensory and motor cortices have been shown to be active when subjects listen to words denoting bodily actions. Kinematic analyses of subje...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2013.09.021
更新日期：2013-10-25 00:00:00
abstract：:We investigated the role of local cutaneous signals and of co-contraction of antagonists in repeated judgements of heaviness of weights lifted by flexor pollicis longus. A reference weight lifted by right thumb flexion was matched to a weight lifted by left thumb flexion. Anaesthesia of the right thumb, or of the supe...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(95)12179-x
更新日期：1995-12-15 00:00:00
abstract：:A calcium-dependent potassium channel K(Ca) has been isolated in mouse motoneurons. With physiological concentrations of potassium across inside-out patches, a 100 pS K(Ca) channel was activated when the bath solution of Ca2+ was in excess of 1 microM. Introduction of the drug tedisamil, a blocker of repolarizing pota...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(92)90746-t
更新日期：1992-09-14 00:00:00
abstract：:The serotonergic dorsal raphé nucleus (DRN) expresses glucocorticoid receptors (GR), and systemic glucocorticoids have been shown to regulate expression and activity of tryptophan hydroxylase isoform 2, the rate-limiting enzyme for serotonin synthesis in brain. We have used intra-DRN injection of pseudotyped adeno-ass...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2017.11.041
更新日期：2018-02-05 00:00:00
abstract：:The epsilon4 allele of the apolipoprotein E gene (APOE) has repeatedly been associated with increased risk for Alzheimer's disease (AD). Bullido and colleagues recently identified a polymorphism in the promoter region of the APOE gene (-491A/T) and found that -491A homozygosity predicted AD independently of APOE epsil...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(98)00567-9
更新日期：1998-08-14 00:00:00
abstract：:Three populations of opiate binding sites (mu, delta and kappa) could be distinguished by displacement studies with the mu-agonist (D)-Ala2-MePhe4-Glyol5-enkephalin and the kappa-agonist [3H](-)-bremazocine in brain membranes of different species. Whereas brain membranes from pig or guinea-pig and human cortical membr...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(82)90417-7
更新日期：1982-06-30 00:00:00
abstract：:Recent findings from our and other laboratories indicate that cell cycle-related phenomena may play a key role in the formation of Alzheimer-type pathology and neuronal cell death in both Alzheimer's and cerebro-vascular diseases. In this study we examine the expression patterns of cyclins A, B1, D1 and E in neuronal ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(99)00509-1
更新日期：1999-08-13 00:00:00
abstract：:Using specific radioimmunoassays, melatonin was quantified in the aqueous humor and serum of male pigmented rabbits adapted to 12L:12D with lights on at 06.00 h. Melatonin concentrations in the aqueous humor at 08.00 and 16.00 h were similar (mean: 5.23 pg/ml) and significantly lower than those at 22.00 and 01.00 h (m...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(90)90092-n
更新日期：1990-08-24 00:00:00
abstract：:Light microscopic autoradiographic localization of specific recognition sites for thyrotropin-releasing hormone (TRH) was determined on thin sections of rat brain using the potent analogue [3H](3-Me-His2)-TRH ([3H]MeTRH). Microdensitometric analysis of the relative optical densities of TRH receptor labelling revealed ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(85)90600-7
更新日期：1985-10-10 00:00:00
abstract：:The longest central nervous system (CNS) specific isoform of the major myelin-associated inhibitor of neurite growth, Nogo-A, has previously been shown to be expressed largely in oligodendrocytes. Using an antibody raised against a recombinant fusion protein comprising amino acids 223-399 of Nogo-A, we show in this re...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(02)00528-1
更新日期：2002-08-16 00:00:00
abstract：:The possible involvement of 2-amino-4-phosphonobutyrate (APB) recognition sites in mechanisms enabling the maintenance of long-term potentiation (LTP) was investigated in rat hippocampal slices. The action of D(-)- and L(+)-isomers of APB was tested on orthodromic EPSP and spike responses recorded extracellularly from...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(89)90504-1
更新日期：1989-03-27 00:00:00
abstract：:N-Methyl-D-aspartate (NMDA)-evoked release of [3H]acetylcholine (ACh) from slices of rat brain medial septum/vertical limb of the diagonal band (ms/vdB) was examined. NMDA increased the release of tritium in a concentration-dependent manner and the specific non-competitive NMDA antagonist, MK-801, and the competitive ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(90)90465-l
更新日期：1990-07-31 00:00:00
abstract：:The contribution of NMDA receptors to neuronal hyperexcitability following in vitro ethanol withdrawal was examined using afterdischarges (ADs) in rat dentate gyrus as a model system. ADs were evoked by high frequency stimuli and blocked by N-methyl-D-aspartate (NMDA) receptor antagonists. Ethanol (75 mM) inhibited AD...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(94)91055-3
更新日期：1994-02-14 00:00:00
abstract：:Gangliosides are sialic acid-containing sphingolipids that have promoting effects on the survival, growth, and functions of neurons in the central nervous system. Glial cells including astrocytes assist neurons with regard to their proliferation, maintenance, and function. In the present study, we found that astrocyte...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2010.11.029
更新日期：2011-01-20 00:00:00
abstract：:One of the clinical characteristics of psychogenic tremors (PT) is the disruption or transient cessation of tremor with distractive manoeuvres, including those involving the performance of voluntary movements with the contralateral hand. Seven patients with PT, 11 patients with Parkinson's disease (PD), 10 patients wi...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2004.08.009
更新日期：2004-11-11 00:00:00
abstract：:Silver staining (Bodian procedure) in the nucleus of the sublenticular substantia innominata (SI), also referred to as the nucleus basalis of Meynert, was evaluated in autopsy material from patients with Alzheimer's disease or senile dementia of the Alzheimer type (age at death: mean 69 years, range 63 to 81 years; ti...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(85)90267-8
更新日期：1985-05-23 00:00:00
abstract：:We raised polyclonal antibodies against a gene product responsible for late infantile neuronal ceroid lipofuscinosis (CLN2). By Western blotting, all three antisera recognized the CLN2 protein at approximately 49 kDa in human brain homogenates. Immunohistochemistry using the antisera demonstrated the granular labellin...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(98)00787-3
更新日期：1998-11-27 00:00:00
abstract：:To investigate the working mechanism of the conflict processing system, pairs of colored numbers were sequentially presented on a screen to subjects. They were asked to determine if the magnitude or the color of the two numbers was identical. Event-related potentials (ERPs) were recorded at the same time. A negative p...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(00)01757-2
更新日期：2001-02-09 00:00:00
abstract：:To assess cortical activity during pain perception, regional cerebral blood flow (rCBF) studies were done in humans using single photon emission computed tomography (SPECT) with the radiotracer Tc99m-HMPAO and magnetic resonance imaging localization. Normalized SPECT data were analyzed by region of interest and change...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(92)90088-o
更新日期：1992-06-22 00:00:00
abstract：:The effects of the N-methyl-D-aspartate (NMDA) and non-NMDA receptor antagonists MK-801 and NBQX, respectively, on salivary secretion and retching induced by vagal stimulation were studied in decerebrate dogs. Vagal stimulation induced an increase in salivary secretion and fictive retching. Intra-4th ventricular appli...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(98)00859-3
更新日期：1998-12-11 00:00:00
abstract：:The three tracers horseradish peroxidase (HRP), 4',6-diamidino-2-phenylindol-2HCl (DAPI) and Fast Blue (FB) differ in retrograde labeling of striato-nigral neurons. After a 24 h survival, injection of DAPI into the ventral tegmentum labeled numerous cells throughout the neostriatum, whereas an identical amount of HRP ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(83)90025-3
更新日期：1983-07-29 00:00:00
abstract：:We recently showed that dopamine (DA) and serotonin (5-HT) exert anticonvulsant effects against limbic seizures in rats mediated by hippocampal D(2) and 5-HT(1A) receptor stimulation. For exogenously administered monoamines, anticonvulsant activity was only observed following 70--400% and 80--350% increases in baselin...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2005.07.049
更新日期：2005-12-16 00:00:00
abstract：:Immunohistochemical fluorescent double labeling revealed the coexistence of galanin and substance P in nerve fibers in the mouse nasal mucosa. At the base of and in the epithelium, all galanin fibers also contained substance P, but around the blood vessels and glands, most of them did not. Since substance P fibers in ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(94)90148-1
更新日期：1994-05-23 00:00:00
abstract：:Telmisartan is an angiotensin II type 1 receptor blocker and partial agonist of peroxisome proliferator-activated receptor gamma (PPAR-γ). Here, we investigated the protective capacity of telmisartan against high glucose (HG)-elicited oxidative damage in PC12 cells. The activity of lactate dehydrogenase (LDH), NADPH o...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2013.10.057
更新日期：2014-01-13 00:00:00
abstract：:A peptide consisting of residues 25-35 of the amyloid beta protein was applied to single neurons while monitoring membrane current by whole cell voltage clamp recording. Within minutes of direct exposure of a neuron to the amyloid beta peptide, a paroxysmal increase in neuronal membrane conductance was observed. This ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(93)90519-q
更新日期：1993-02-19 00:00:00
abstract：:Previous neuroimaging studies raised the hypothesis that enhanced activity in the ipsilateral motor cortex (M1) plays a contributing role in the compensation for the motor deficits resulting from a spinal cord injury (SCI). However, it is still unknown whether the activity in the ipsilateral M1 directly contributes to...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2013.07.011
更新日期：2013-09-27 00:00:00
abstract：:Dopamine metabolism and release were determined in the striata of rats sustaining varying damage to the nigrostriatal dopamine (DA) projection. DA metabolism, inferred from concentrations of dihydroxy-phenylacetic acid (DOPAC) or homovanillic acid (HVA), decreased with DA denervation of more than 20%. Dopamine release...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(89)90400-x
更新日期：1989-01-30 00:00:00
abstract：:We have investigated the effect of ethanol upon vasopressin (AVP) content in brain and upon in-vitro release of AVP from the rat median eminence. In-vitro ethanol concentrations (5-25 mM), comparable to behaviorally relevant blood ethanol levels, induce a substantial inhibition of AVP release from the median eminence,...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(86)90400-3
更新日期：1986-06-18 00:00:00
