Abstract:
:We raised polyclonal antibodies against a gene product responsible for late infantile neuronal ceroid lipofuscinosis (CLN2). By Western blotting, all three antisera recognized the CLN2 protein at approximately 49 kDa in human brain homogenates. Immunohistochemistry using the antisera demonstrated the granular labelling in the cytoplasm of cerebral neurons and glial cells. The immunoreactivity on Western blots was absent from the brain of a patient with CLN2. Our results suggest the usefulness of these antibodies for the diagnosis of CLN2, which currently requires demonstration of characteristic ultrastructure by electron microscopy.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Oka A,Kurachi Y,Mizuguchi M,Hayashi M,Takashima Sdoi
10.1016/s0304-3940(98)00787-3subject
Has Abstractpub_date
1998-11-27 00:00:00pages
113-5issue
2eissn
0304-3940issn
1872-7972pii
S0304-3940(98)00787-3journal_volume
257pub_type
杂志文章abstract::Olivary hypertrophy, a combined retrograde and transneuronal reaction, is found in cells of the inferior olive after cerebellar ablation in the cat. Its chief features are central chromatolysis and hypertrophy of the peripherally located granular endoplasmatic reticulum with accumulation of electron-dense material in ...
journal_title:Neuroscience letters
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
pub_type: 杂志文章
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
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更新日期:2005-10-14 00:00:00
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
pub_type: 杂志文章
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更新日期:2012-12-07 00:00:00
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
pub_type: 杂志文章
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更新日期:2004-11-23 00:00:00
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journal_title:Neuroscience letters
pub_type: 杂志文章
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journal_title:Neuroscience letters
pub_type: 杂志文章
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更新日期:2011-01-20 00:00:00