Abstract：
:Restoration of autophagy represents a potential therapeutic target for neurodegenerative disorders, but factors that regulate autophagic flux are largely unknown. When deprived of trophic factors, cultured Purkinje neurons die by an autophagy associated cell death mechanism. The accumulation of autophagic vesicles and cell death of Purkinje neurons is inhibited by insulin-like growth factor, by a mechanism that enhances autophagic vesicle turnover. In this report, we identify Rab7 as an IGF-I regulated target during neuronal autophagy. Purkinje neurons transfected with EGFP-Rab7-WT and constitutively active EGFP-Rab7-Q67L contained few RFP-LC3 positive autophagosomes and little co-localization with GFP-Rab7 under control conditions. Upon induction of autophagy, RFP-LC3 positive autophagosomes increased and co-localized with GFP-Rab7. Conversely, expression of the dominant negative mutant EGFP-Rab7-T22N increased the accumulation of autophagosomes under control conditions, which accumulated even further during trophic factor withdrawal. There was no vesicular co-localization between Rab7-T22N and RFP-LC3 under control or trophic factor withdrawal conditions. During prolonged trophic factor withdrawal, a condition that leads to the accumulation of autophagic vesicles and cell death, Rab7 activity decreased significantly. IGF-I, added at the time of trophic factor withdrawal, prevented the deactivation of Rab7 and increased the interaction of Rab7 with its interacting protein (RILP), restoring autophagic flux. These results provide a novel mechanism by which IGF-I regulates autophagic flux during neuronal stress.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Bains M,Zaegel V,Mize-Berge J,Heidenreich KAdoi
10.1016/j.neulet.2010.09.018subject
Has Abstractpub_date
2011-01-20 00:00:00pages
112-7issue
2eissn
0304-3940issn
1872-7972pii
S0304-3940(10)01209-7journal_volume
488pub_type
杂志文章abstract：:The molecular regulation of seizure-induced neuronal death may involve interactions between proteins of the Bcl-2 and 14-3-3 families. To further examine these pathways we performed subcellular fractionation on hippocampi obtained following a brief period of status epilepticus in the rat. Western blotting determined s...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2003.11.048
更新日期：2004-02-19 00:00:00
abstract：:The effects of gonadal steroids on serotonin (5-HT) synthesis and metabolism in the early developing brain were investigated. Seven-day-old (7 days post-hatch) tilapia, Oreochromis mossambicus were continuously treated with 17beta-estradiol (E2), methyltestosterone (MT) and para-chlorophenylalanine (p-PCA) up to the a...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(99)00160-3
更新日期：1999-04-02 00:00:00
abstract：:(1) This study shows the effects of bicuculline pretreatment on the GABAergic system in certain areas of the rat brain after acute ether and cold stress. (2) The spontaneous locomotor activity was diminished by either ether stress or cold stress or bicuculline. (3) Acute ether stress enhanced GABA concentration in the...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(94)90863-x
更新日期：1994-07-18 00:00:00
abstract：:The combination of ethanol and cocaine is commonly abused by human addicts which has serious clinical consequences. Here, the effects of separately and concurrently administered ethanol and 'binge' cocaine on kappa opioid receptor (KOR) mRNA in the ventral tegmental area (VTA) and nucleus accumbens (NAc) of rats were ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(99)00675-8
更新日期：1999-11-05 00:00:00
abstract：:Recent studies have shown that aging, psychiatric and neurologic diseases, and dopaminergic blockade all result in altered brain network efficiency. We investigated the efficiency of human brain functional networks as measured by fMRI in individuals with idiopathic Parkinson's disease (N=14) compared to healthy age-ma...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2011.05.030
更新日期：2011-07-15 00:00:00
abstract：:Immunoreaction for vasoactive intestinal polypeptide (VIP) in the ependymal cells was investigated using two different commercially available polyclonal antisera for VIP. Immunostaining with an anti-VIP serum showed strong reaction products in the ependymal cells of the central canal and the third ventricle, in additi...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(89)90171-7
更新日期：1989-12-04 00:00:00
abstract：:Mutations in DJ-1 cause familial Parkinson's disease (PD). The expression pattern of DJ-1 in the brain remains controversial. In the present study, we used DJ-1 deficient mice as negative controls and examined DJ-1 mRNA expression in mouse brains. In sequential double labeling on the same sections, in situ hybridizati...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2009.09.024
更新日期：2009-11-20 00:00:00
abstract：:To determine how position sense depends on the functional state of the receptors involved, we assessed the accuracy of knee position sense before and after a moderate exercise on a cycle ergometer. Measurements were done on 32 healthy subjects with four protocols combining two tasks (intramodal: using the contralatera...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(00)01297-0
更新日期：2000-08-04 00:00:00
abstract：:Although the mechanisms of visual short term memory (VSTM) are extensively investigated in the recent decade, how we compare the representation stored in VSTM to the perceptual input to detect and process the mismatch information remains largely unclear. The current study explored whether there is an ERP component tra...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2011.01.001
更新日期：2011-03-10 00:00:00
abstract：:New psychoactive substances (NPSs), i.e., newly designed substances with chemical residues that are slightly different from those of known psychoactive substances, have been emerging since the late 2000s, and social problems related to the use of these substances are increasing globally. Two such NPSs are 4-chloro-2,5...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2018.04.009
更新日期：2018-05-29 00:00:00
abstract：:Antidepressive medication and electroconvulsive therapy (ECT) increase hippocampal neurogenesis by promoting expression of trophic factors, including brain-derived neurotrophic factor (BDNF) and vascular endothelial growth factor (VEGF). The aims were to test for an association between the VEGF 2578 C/A polymorphism a...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2010.04.039
更新日期：2010-06-25 00:00:00
abstract：:To investigate the capacity of the central nervous system to integrate and differentiate two different muscular fatigue states, the present study examines the changes on multi-joint movement organization following muscular fatigue of elbow extensor muscles (triceps brachii) induced by voluntary versus electrically ind...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2006.04.025
更新日期：2006-07-31 00:00:00
abstract：:Whole-cell patch-clamp recordings (WCR) were made from sympathetic preganglionic neurons (SPN) in neonate rat spinal cord slices. SPN were identified histologically by filling them with the fluorescent dye Lucifer Yellow contained within the patch pipette solution. Current clamp recordings were obtained from SPN with ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(91)90405-i
更新日期：1991-09-16 00:00:00
abstract：:8-OH-DPAT is a 5-HT(1A/7) receptor agonist that enhances behavioral recovery after traumatic brain injury (TBI). This study is a first attempt to decipher whether the benefits induced by 8-OH-DPAT after TBI are mediated by 5-HT(1A) or 5-HT(7) receptors. A single i.p. injection of 8-OH-DPAT (0.5 mg/kg) alone or co-admi...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2012.03.033
更新日期：2012-05-02 00:00:00
abstract：:The present study evaluated the relationship between asymmetry of the prefrontal cortex activity and the automatic nervous system (ANS) response during a mental arithmetic (MA) task. Employing near infrared spectroscopy, we compared cerebral blood oxygenation changes in the right and left prefrontal cortices during a ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2004.07.076
更新日期：2004-10-07 00:00:00
abstract：:Based on its proposed ability to block the effect of selective serotonin reuptake inhibitors (SSRIs) on the firing rate of serotonergic neurons, the 5-HT1A partial agonist/beta-adrenergic antagonist pindolol has been examined in clinical trials for its ability to enhance the efficacy of SSRIs. However, varying results...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2003.10.039
更新日期：2004-01-23 00:00:00
abstract：:Alpha-tocopherol and its derivatives have been shown to be effective in reducing cerebral ischemia-induced brain damage. However, the effects of other vitamin E isoforms have not been characterized. In the present study, we investigated the effects of six different isoforms of vitamin E on the ischemic brain damage in...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(02)01293-4
更新日期：2003-01-30 00:00:00
abstract：:Surround inhibition (SI) is a neural mechanism to focus neuronal activity and facilitate selective motor execution (ME). The aim of the present study was to investigate whether SI is also generated during motor imagery (MI). Furthermore, we investigated whether the extent of SI during MI depends on the strength of SI ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2016.07.012
更新日期：2016-08-26 00:00:00
abstract：:The presence and distribution of the presumed pan-neural marker protein gene product 9.5 (PGP)- and peptide-immunoreactive (ir) nerve fibers in alveolar walls of various species was investigated by light microscopic single and double staining immunohistochemistry. PGP-, tachykinin (TK)-, and calcitonin gene-related pe...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(91)90498-i
更新日期：1991-12-16 00:00:00
abstract：:Peroxisome proliferator-activated receptors (PPARs) are ligand-dependent transcription factors which belong to the nuclear receptor family. We examined whether PPARalpha agonists and resveratrol, a polyphenol contained in grapes, protect the brain against ischemia. To investigate whether resveratrol activates PPARs, w...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2003.09.001
更新日期：2003-12-11 00:00:00
abstract：:Adult mouse sciatic nerves (SNs) with attached dorsal root ganglia (DRG) were analysed for the presence of mitogen activated protein kinase (MAP-K) during normal and regenerative conditions. By immunohistochemistry, MAP-K was found to be present in the normal nerve at low levels in both Schwann cells and DRG nerve cel...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(95)12066-d
更新日期：1995-11-10 00:00:00
abstract：:Alzheimer's disease has become one of the most impacting disorders since world population is rapidly aging. MicroRNA-125b plays a crucial role in many cellular processes and pathologies, but, to date, its role in Alzheimer's disease is controversial. In this study, we demonstrated, for the first time, that the down re...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2016.05.049
更新日期：2016-08-26 00:00:00
abstract：:Immunolocalization of 14-3-3 protein isoforms in relation to Pick bodies in Pick body disease (PBD) brains was investigated. Weakly granular immunoreactivity of 14-3-3 proteins was found in neurons in control subjects and in Pick body disease brains. In addition to this granular immunoreactivity, many Pick bodies were...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2004.08.079
更新日期：2004-11-23 00:00:00
abstract：:Previously published data have indicated that in the rat, unlike other species examined, the kidney is not supplied by sensory nerves containing substance P (SP). As part of a study of reflex control of renal function in the rat, we have now reassessed this situation. Many fine, varicose, SP-immunoreactive nerve fiber...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(85)90241-1
更新日期：1985-09-30 00:00:00
abstract：:The aim of the present study was to determine if pre-exposure to high doses of cocaine can subsequently alter the rewarding effects of this drug. Adult male mice received a pretreatment of physiological saline, or 12.5 or 25 mg/kg of cocaine (one injection a day for five days). After an interval of six days without in...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2012.03.044
更新日期：2012-05-10 00:00:00
abstract：:Huntingtin-associated protein 1 (HAP1) interacts with the product of the Huntington's disease gene. To investigate the function of Hap1 in development and in the adult mouse, we have examined the expression of Hap1 by northern analysis and in situ hybridization histochemistry. Hap1 expression is first detected in the ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(00)00872-7
更新日期：2000-03-17 00:00:00
abstract：:Using an antiserum (AS) raised against dynorphin B (DYN B), we revealed immunoreactive neurons in different nuclei of the rat hypothalamus as well as a population of neurons scattered in the lateral areas of the posterior hypothalamus. This population corresponds to that previously shown to be specifically recognized ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(93)90447-s
更新日期：1993-06-25 00:00:00
abstract：:Occurrence of neurocalcin, a calcium-binding protein with three EF hand motifs, was examined immunohistochemically in the rat olfactory epithelium and vomeronasal organ epithelium. Immunoreactivity was detected in receptor cells in these epithelia. Immunoreactions were distributed in cytoplasm associated with outer mi...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(95)11568-2
更新日期：1995-05-19 00:00:00
abstract：:Interleukin-6 (IL-6) has been shown to have potent neurotrophic activity on peripheral and central neurons in vitro. However, it remains to be determined whether or not IL-6 rescues hippocampal CA1 neurons from lethal ischemia and prevents ischemia-induced learning disability. In the present in vivo study, we infused ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(96)12340-5
更新日期：1996-02-02 00:00:00
abstract：:It has been well documented that the activation of Akt1 and JNK pathways are involved in the neuronal cell death in cerebral ischemia. In this study, we describe a novel interaction between Akt1 and JNK interacting protein 1 (JIP-1). We first detected the interaction of Akt1 and JIP-1 in hippocampus at various time po...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2005.08.057
更新日期：2006-01-09 00:00:00
