Abstract:
:Whole-cell patch-clamp recordings (WCR) were made from sympathetic preganglionic neurons (SPN) in neonate rat spinal cord slices. SPN were identified histologically by filling them with the fluorescent dye Lucifer Yellow contained within the patch pipette solution. Current clamp recordings were obtained from SPN with a potassium based pipette solution. The cells exhibited many of the characteristic properties of SPN seen previously with intracellular recordings in both the rat and the cat. However, we found an order of magnitude increase in both cell input resistance (950 M omega) and time constant (118 ms) over those seen with conventional recordings. We believe these values approximate better the situation in intact cells, and will have a vital bearing upon how SPN integrate inputs. We conclude that WCR in spinal cord slices provides a powerful tool for investigating the cellular properties of SPN.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Pickering AE,Spanswick D,Logan SDdoi
10.1016/0304-3940(91)90405-isubject
Has Abstractpub_date
1991-09-16 00:00:00pages
237-42issue
2eissn
0304-3940issn
1872-7972pii
0304-3940(91)90405-Ijournal_volume
130pub_type
杂志文章abstract::Small iontophoretic applications of wheat germ agglutinin-conjugated to horseradish peroxidase have been used to simultaneously observe, in the rat, the afferent and efferent connections of small parabrachial subregions in the same animal. The results demonstrate that a restricted region centered on the external porti...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(89)90664-2
更新日期:1989-05-22 00:00:00
abstract::Canine degenerative myelopathy (DM) is an adult-onset progressive and fatal neurodegenerative disorder. Superoxide dismutase 1 (SOD1) mutations have been reported in affected dogs and immunohistochemical analyses revealed the accumulation of mutant SOD1 (E40K) in spinal neurons and astrocytes. Therefore, this disease ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2018.09.040
更新日期:2018-11-20 00:00:00
abstract::Painful vincristine (VCR) neuropathy is a frequent and dose-limiting problem in cancer treatment. Here, we investigated how pain behavior is modulated in mice lacking the serotonin transporter (5-HTT-/- mice) after inducing neuropathy by intraperitoneal injections of VCR. We used standard tests for evoked pain, high p...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2011.03.035
更新日期:2011-05-16 00:00:00
abstract::This investigation was designed to determine the responses of neurons in the posterior group of nuclei (PO) to tooth pulp stimulation. Eighteen tooth pulp-driven (TPD) neurons were recorded in 9 cats anesthetized with nitrous oxide and halothane, 14 of them in the medial part (POM) and the remainder in the lateral par...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(88)90091-2
更新日期:1988-11-11 00:00:00
abstract::Several studies have suggested that the activity of nitric oxide synthase (NOS) may be involved in the regulation of food intake in the genetically obese Zucker rats. In the present study, we investigated the expression of NOS in various hypothalamic regions of obese and lean Zucker rats using nicotinamide adenine din...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(00)01428-2
更新日期:2000-09-29 00:00:00
abstract::We investigated the relation between visual feedback and the degree of structure versus randomness in the variability of single-digit, isometric force output. Participants were instructed to maintain a constant level of force during the presence or absence of visual feedback about force output. The structure of force ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2012.02.002
更新日期:2012-03-28 00:00:00
abstract::Three dopamine receptor mRNAs were detected by reverse polymerase chain reaction in the rat forebrain during ontogeny. The D1 receptor mRNA gave a faint signal from gestational day 11 (G11) on, while the D1b receptor was not detectable before G12. The D3 receptor mRNA was slightly expressed at G11 and clearly present ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(93)90680-j
更新日期:1993-05-28 00:00:00
abstract::Vigabatrin (VGB, gamma-vinyl-gamma-aminobutyric acid (GABA)), an irreversible inhibitor of GABA transaminase, increases regional inhibitory effects by elevating GABA concentration and reducing glutamate synthesis. In the present study, changes in glutamate dehydrogenase (GDH) activity and its immunoreactivity in the s...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(03)00072-7
更新日期:2003-04-10 00:00:00
abstract::Intracellular recordings were made from primary sensory hair cells located on the dorsal side of the anterior crista segment of the squid statocyst. These hair cells were electrophysiologically identified by the occurrence of an antidromic action potential after electrical stimulation of the crista nerve. Two types of...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(93)90300-a
更新日期:1993-10-29 00:00:00
abstract::Gabaculine, a conformationally restricted analogue of GABA, is (i) a moderately potent inhibitor (IC(50) 69 muM) of the sodium-dependent uptake of GABA in rat brain slices, (ii) ineffective at 100 muM as an inhibitor of the sodium-independent binding of GABA to membranes from rat brain, (iii) a relatively weak inhibit...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(77)90124-0
更新日期:1977-01-01 00:00:00
abstract::We evaluated the neuroprotective effects of systemically administered magnesium against N-methyl-D-aspartate (NMDA)-mediated brain injury in perinatal rats. Postnatal day (PND) 7 rats received unilateral intrastriatal injections of 25 nmol NMDA followed 15 min later by single or multiple doses of magnesium intraperito...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(90)90569-u
更新日期:1990-02-05 00:00:00
abstract::Remacemide is a potential anticonvulsant drug with an active metabolite, desglycinyl-remacemide (DGR). Both moieties have been reported to block neuronal Na(+) channels and the N-methyl-D-aspartate (NMDA) subtype of glutamate receptor. The effects of remacemide and DGR on zero Mg(2+)/4-aminopyridine-induced epileptifo...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(01)02511-3
更新日期:2002-03-15 00:00:00
abstract::Alpha-synuclein has been implicated in the pathology of certain neurodegenerative diseases, including Parkinson disease (PD) and dementia with Lewy bodies (LBs). Overexpression of human alpha-synuclein in neuronal cells reduces cell viability, but the precise cellular and molecular mechanisms remain poorly understood....
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2007.02.025
更新日期:2007-04-18 00:00:00
abstract::Rat neocortical slices maintained in Mg2(+)-free Krebs medium developed spontaneous paroxysmal discharges which were attenuated or suppressed by the gamma-aminobutyric acid-B (GABAB) receptor agonist baclofen, occasionally accompanied by a slight hyperpolarisation, and antagonised by the specific GABAB-receptor antago...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(90)90557-p
更新日期:1990-02-05 00:00:00
abstract::Using the novel substituted benzamide drug [3H]raclopride in combination with in vitro receptor autoradiography, the distribution of dopamine D-2 receptors was studied in the monkey brain. Highest densities of D-2 receptors are present in dopamine-rich areas and the distribution shows the following rank order: caudatu...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(86)90170-9
更新日期:1986-05-06 00:00:00
abstract::Cholinergic influences on attention are well documented and recent evidence indicates that genetic variation in the alpha4beta2 nicotinic receptor affects attentional networks of the brain. Several behavioral and electrophysiological studies have shown that a common polymorphism in the CHRNA4 gene (rs1044396) affects ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2009.02.029
更新日期:2009-04-10 00:00:00
abstract::Microinjection into the dorsolateral pontine tegmentum of the muscarinic agonist bethanechol, leading to activation of cholinoceptive pontine reticular formation (pRF) neurons and the related medullary inhibitory reticulospinal system, did not modify the threshold, latency and amplitude of the forelimb flexion elicite...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(90)90431-8
更新日期:1990-06-22 00:00:00
abstract::Amphiphysin II (Amph2) is known to undergo rapid dephosphorylation and phosphorylation at nerve terminals. After in vivo electroconvulsive shock (ECS) in the rat cerebellum, we found an electrophoretic mobility retardation of Amph2, which suggested an increased degree of phosphorylation above the non-stimulated level....
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(02)00748-6
更新日期:2002-09-20 00:00:00
abstract::Single photon-induced transient currents, called quantum bumps were stimulated by short flashes in dark-adapted ventral nerve photoreceptors of Limulus. Flash intensities were set to activate 3 or more bumps. In most cases, current bumps were activated with a constant rate. The frequency of bump occurrence was between...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(92)90725-m
更新日期:1992-09-14 00:00:00
abstract::125I-Staphylococcal protein A was used to visualize immunoreactive cell antigen in rat brain and pituitary by autoradiography. Autoradiograms of rat brain sections generated with 125I-protein A were clear and showed low background signals. We were able to visualize neural structures containing tyrosine hydroxylase or ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(88)90368-0
更新日期:1988-06-29 00:00:00
abstract::Pineal gland N-acetyltransferase (NAT) activity and pineal and serum levels of melatonin declined linearly in albino rats exposed to different irradiances (low, 170 microW/cm2; moderate, 420 microW/cm2; high, 1040 microW/cm2) red light during the middle of the night. High intensity red light (1040 microW/cm2) was as e...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(93)90346-m
更新日期:1993-01-04 00:00:00
abstract::Axons in chronically demyelinated spinal cord lesions, induced by ethidium bromide injection, display patches of intramembranous particles and indentations resembling nodal and paranodal axolemmal specializations respectively. Both occur in the marginal region of the lesions where the demyelinated axons are intimately...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(84)90015-6
更新日期:1984-07-27 00:00:00
abstract::Using collision tests of orthodromically and antidromically generated spikes, we studied the axonal pathways, axonal projection levels, and soma location of anterior semicircular canal (AC) nerve-activated vestibulospinal neurons in decerebrate cats. AC nerve-activated vestibulospinal neurons (n=74) were mainly locate...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2006.06.024
更新日期:2006-10-02 00:00:00
abstract::Interleukin-6 (IL-6) has been shown to have potent neurotrophic activity on peripheral and central neurons in vitro. However, it remains to be determined whether or not IL-6 rescues hippocampal CA1 neurons from lethal ischemia and prevents ischemia-induced learning disability. In the present in vivo study, we infused ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(96)12340-5
更新日期:1996-02-02 00:00:00
abstract::Methylated beta-carboline compounds are mammalian indole metabolites that we have proposed to be endogenous neurotoxins due to their structural similarity to MPP+, the active oxidized product of the dopaminergic toxin, N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Several laboratories have demonstrated that MPP...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(89)90645-9
更新日期:1989-11-06 00:00:00
abstract::It is known that 5HTR2A (rs6311) receptors have high concentration in the cortical layer-5 pyramidal neurons and that these receptors play an important role in the modulation of neurocognitive functions. For example, layer-5 pyramidal neurons mediate cellular level integrative interaction of primary sensory afferent s...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2014.10.015
更新日期:2015-01-01 00:00:00
abstract::UBB+1 protein is an aberrant ubiquitin associated with progressive supranuclear palsy (PSP). It leads to proteasome inhibition, heat-shock protein (HSP) expression and apoptosis in cell cultures. Despite UBB+1 polyubiquitination (an indication of proteasome inhibition), we demonstrate that UBB+1 and HSP40/HSP70 immuno...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2003.12.127
更新日期:2004-04-08 00:00:00
abstract::Amyloid beta-peptide (Abeta) deposition in brain is important in the development of Alzheimer's disease (AD). Neprilysin (NEP) appears to be the major Abeta degrading enzyme in vivo and reduced mRNA levels of NEP correlates with increased plaque density. We hypothesized that alterations in the NEP promoter region may ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(02)01300-9
更新日期:2003-02-06 00:00:00
abstract::Parkinson's disease (PD) is characterized by the selective loss of dopaminergic (DAergic) neurons in the substantia nigra and the subsequent depletion of dopamine (DA). This study assessed the protective effects of costunolide on DA-induced apoptosis in human DAergic SH-SY5Y cells, and its regulation of DA metabolism-...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2011.10.037
更新日期:2012-01-24 00:00:00
abstract::Motor and sensory neuropathies with the clinical features of HMSN III (Dejerine-Sottas syndrome, DSS) are etiologically related to heterozygous mutations in either peripheral myelin protein-22 (PMP22) or myelin protein zero (MPZ). Heterozygous mutations in either of these two genes are also responsible for other hered...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(97)00887-2
更新日期:1998-01-02 00:00:00