Abstract:
:In the molecular farming area, transient expression approaches for pharmaceutical proteins production, mainly recombinant monoclonal antibodies and vaccines, were developed almost two decades ago and, to date, these systems basically depend on Agrobacterium-mediated delivery and virus expression machinery. We survey here the current state-of-the-art of this research field. Several vectors have been designed on the basis of DNA- and RNA-based plant virus genomes and viral vectors are used both as single- and multicomponent expression systems in different combinations depending on the protein of interest. The obvious advantages of these systems are ease of manipulation, speed, low cost and high yield of proteins. In addition, Agrobacterium-mediated expression also allows the production in plants of complex proteins assembled from subunits. Currently, the transient expression methods are preferential over any other transgenic system for the exploitation of large and unrestricted numbers of plants in a contained environment. By designing optimal constructs and related means of delivery into plant cells, the overall technology plan considers scenarios that envisage high yield of bioproducts and ease in monitoring the whole spectrum of upstream production, before entering good manufacturing practice facilities. In this way, plant-derived bioproducts show promise of high competitiveness towards classical eukaryotic cell factory systems.
journal_name
Expert Rev Vaccinesjournal_title
Expert review of vaccinesauthors
Komarova TV,Baschieri S,Donini M,Marusic C,Benvenuto E,Dorokhov YLdoi
10.1586/erv.10.85subject
Has Abstractpub_date
2010-08-01 00:00:00pages
859-76issue
8eissn
1476-0584issn
1744-8395journal_volume
9pub_type
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journal_title:Expert review of vaccines
pub_type: 杂志文章,meta分析
doi:10.1586/erv.09.161
更新日期:2010-02-01 00:00:00
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journal_title:Expert review of vaccines
pub_type: 杂志文章,评审
doi:10.1586/14760584.1.2.215
更新日期:2002-08-01 00:00:00
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journal_title:Expert review of vaccines
pub_type: 杂志文章,评审
doi:10.1586/14760584.4.6.843
更新日期:2005-12-01 00:00:00
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journal_title:Expert review of vaccines
pub_type: 杂志文章,评审
doi:10.1586/14760584.2.6.763
更新日期:2003-12-01 00:00:00
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journal_title:Expert review of vaccines
pub_type: 杂志文章,评审
doi:10.1586/14760584.7.2.209
更新日期:2008-03-01 00:00:00
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journal_title:Expert review of vaccines
pub_type: 杂志文章,评审
doi:10.1586/erv.11.66
更新日期:2011-08-01 00:00:00
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journal_title:Expert review of vaccines
pub_type: 杂志文章,评审
doi:10.1586/14760584.4.2.167
更新日期:2005-04-01 00:00:00
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journal_title:Expert review of vaccines
pub_type: 杂志文章,评审
doi:10.1586/14760584.6.5.809
更新日期:2007-10-01 00:00:00
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journal_title:Expert review of vaccines
pub_type: 杂志文章,评审
doi:10.1586/14760584.2.1.85
更新日期:2003-02-01 00:00:00
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journal_title:Expert review of vaccines
pub_type: 传,历史文章,面试
doi:10.1586/14760584.2013.836913
更新日期:2013-10-01 00:00:00
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journal_title:Expert review of vaccines
pub_type: 杂志文章,评审
doi:10.1586/14760584.3.4.339
更新日期:2004-08-01 00:00:00
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journal_title:Expert review of vaccines
pub_type: 杂志文章,评审
doi:10.1586/14760584.5.5.669
更新日期:2006-10-01 00:00:00
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journal_title:Expert review of vaccines
pub_type: 杂志文章,评审
doi:10.1586/14760584.2015.981533
更新日期:2015-03-01 00:00:00
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journal_title:Expert review of vaccines
pub_type: 杂志文章,评审
doi:10.1586/erv.11.21
更新日期:2011-04-01 00:00:00
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journal_title:Expert review of vaccines
pub_type: 杂志文章,评审
doi:10.1080/14760584.2019.1580578
更新日期:2019-03-01 00:00:00
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journal_title:Expert review of vaccines
pub_type: 杂志文章,评审
doi:10.1586/erv.11.166
更新日期:2012-01-01 00:00:00
abstract::Conjugate vaccines using diphtheria toxoid variant (CRM(197)), diphtheria toxoid and tetanus toxoid (TT) as carrier protein may induce immune interactions (interference or impairment as measured by lower antibody levels, or enhancement [higher antibody levels]) when coadministered with other vaccines. Immune enhanceme...
journal_title:Expert review of vaccines
pub_type: 杂志文章,评审
doi:10.1586/erv.11.142
更新日期:2011-11-01 00:00:00
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journal_title:Expert review of vaccines
pub_type: 杂志文章,评审
doi:10.1586/14760584.2016.1130628
更新日期:2016-05-01 00:00:00
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journal_title:Expert review of vaccines
pub_type: 杂志文章,评审
doi:10.1586/erv.10.17
更新日期:2010-04-01 00:00:00
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journal_title:Expert review of vaccines
pub_type: 杂志文章,评审
doi:10.1586/14760584.2015.1026808
更新日期:2015-06-01 00:00:00
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journal_title:Expert review of vaccines
pub_type: 杂志文章,评审
doi:10.1586/14760584.2015.1037744
更新日期:2015-07-01 00:00:00
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journal_title:Expert review of vaccines
pub_type:
doi:10.1586/14760584.7.6.721
更新日期:2008-08-01 00:00:00
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journal_title:Expert review of vaccines
pub_type: 杂志文章,评审
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更新日期:2006-02-01 00:00:00
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journal_title:Expert review of vaccines
pub_type: 杂志文章,评审
doi:10.1080/14760584.2019.1580579
更新日期:2019-04-01 00:00:00
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journal_title:Expert review of vaccines
pub_type: 杂志文章,评审
doi:10.1080/14760584.2018.1445530
更新日期:2018-04-01 00:00:00
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journal_title:Expert review of vaccines
pub_type: 杂志文章,评审
doi:10.1080/14760584.2016.1176533
更新日期:2016-10-01 00:00:00
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journal_title:Expert review of vaccines
pub_type: 杂志文章,评审
doi:10.1586/14760584.3.2.141
更新日期:2004-04-01 00:00:00
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journal_title:Expert review of vaccines
pub_type: 杂志文章,评审
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更新日期:2016-01-01 00:00:00
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journal_title:Expert review of vaccines
pub_type: 杂志文章,评审
doi:10.1586/14760584.2.2.305
更新日期:2003-04-01 00:00:00
abstract::Efforts to develop a meningococcal vaccine that will include coverage against serogroup B disease have largely focused on outer membrane protein antigens. The protection offered by currently licensed outer membrane vesicle (OMV) vaccines is specific to epidemic strains as their immunodominant antigens are highly varia...
journal_title:Expert review of vaccines
pub_type: 杂志文章,评审
doi:10.1586/erv.11.10
更新日期:2011-03-01 00:00:00