Abstract：
:Neural stem cells are a multipotent population of tissue-specific stem cells with a broad but limited differentiation potential. However, recent studies have shown that over-expression of the pluripotency gene, Oct4, alone is sufficient to initiate a process by which these can form 'induced pluripotent stem cells' (iPS cells) with the same broad potential as embryonic stem cells. This led us to examine the expression of Oct4 in endogenous neural stem cells, as data regarding its expression in neural stem cells in vivo are contradictory and incomplete. In this study we have therefore analysed the expression of Oct4 and other genes associated with pluripotency throughout development of the mouse CNS and in neural stem cells grown in vitro. We find that Oct4 is still expressed in the CNS by E8.5, but that this expression declines rapidly until it is undetectable by E15.5. This decline is coincident with the gradual methylation of the Oct4 promoter and proximal enhancer. Immunostaining suggests that the Oct4 protein is predominantly cytoplasmic in location. We also found that neural stem cells from all ages expressed the pluripotency associated genes, Sox2, c-Myc, Klf4 and Nanog. These data provide an explanation for the varying behaviour of cells from the early neuroepithelium at different stages of development. The expression of these genes also provides an indication of why Oct4 alone is sufficient to induce iPS formation in neural stem cells at later stages.
journal_name
J Anatjournal_title
Journal of anatomyauthors
Lee SH,Jeyapalan JN,Appleby V,Mohamed Noor DA,Sottile V,Scotting PJdoi
10.1111/j.1469-7580.2010.01269.xsubject
Has Abstractpub_date
2010-09-01 00:00:00pages
203-13issue
3eissn
0021-8782issn
1469-7580pii
JOA1269journal_volume
217pub_type
杂志文章abstract：:The study of the underlying mechanisms by which cells respond to mechanical stimuli, i.e. the link between the mechanical stimulus and gene expression, represents a new and important area in the morphological sciences. Several cell types ('mechanocytes'), e.g. osteoblasts and fibroblasts as well as smooth, cardiac and...
journal_title：Journal of anatomy
pub_type： 杂志文章,评审
doi：10.1046/j.1469-7580.1999.19430323.x
更新日期：1999-04-01 00:00:00
abstract：:The occurrence of pneumatisation in the skull of the adult domestic fowl was investigated in eight birds by gross and histological examination. It was found to occur regularly throughout the neurocranium and in the quadrate and variably in the mandible, but to be absent in the facial skeleton. Close agreement was foun...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1984-06-01 00:00:00
abstract：:Hydrocortisone administration in vivo to neonatal mice for seven days led to a significant increase in both the size and the labelling index of extra-adrenal chromaffin tissue (as represented by the para-aortic body) of 8 days old mice. In untreated animals at this age, the para-aortic body was in most cases too small...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1986-02-01 00:00:00
abstract：:Anatomical specimens used in human or veterinary anatomy laboratories are usually prepared with formaldehyde (a cancerous and teratogenic substance), glycerin (an expensive and viscous fluid), or ethanol (which is flammable). This research aimed to verify the viability of an aqueous 30% sodium chloride solution for pr...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.12185
更新日期：2014-07-01 00:00:00
abstract：:The role of the neural arch in weight transmission in the cervical and upper thoracic regions of the vertebral column has been investigated. Measurements at the levels of C2, C4, C6, C7, T1 and T5 vertebrae were made in 44 adult male vertebral columns. At each level, the area of the inferior surface of the body was co...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1986-10-01 00:00:00
abstract：:The morphology of the cartilage-marrow interface in chick embryo tibiae has been studied from Day 11 to Day 14. The cartilage-marrow interface did not present a uniform aspect and three different areas could be defined. Most of the interface was lined by fibroblast-like cells, macrophage-like cells and multinucleate g...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1988-10-01 00:00:00
abstract：:The unusual occurrence of skeletal muscle in the pineal gland of the bat Rhinopoma microphyllum is described. Individual muscle fibres were centrally placed and were incompletely encapsulated by a labyrinthine network of widened spaces filled with electron-dense material. The 2.4 micron long sarcomeres consisted of we...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：:The number of large neurons in the lateral vestibular nucleus of the mouse brain decreases in number after 25 months of age, from 488 at 25 months to 298 at 31 months. The number of small neurons in the nucleus remains constant up to 31 months of age. Large neurons accumulate lipofuscin from 25 months and their mean n...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1989-10-01 00:00:00
abstract：:We sought to study the effect of synovectomy on the surface morphology of articular cartilages of the rabbit knee using scanning electron microscopy (SEM). Fifteen rabbits were surgically synovectomised and allowed to regenerate their synovia during time intervals ranging from 3 to 44 wk. Cartilage specimens were shav...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1046/j.1469-7580.1998.19230343.x
更新日期：1998-04-01 00:00:00
abstract：:The present paper describes a novel structure, termed the peri-albumen layer, in the egg-envelopes of the quail Coturnix japonica. It reacts with Alcian blue and exists between the egg white and the shell membrane. Ultrastructurally, it is of fine granular structure and forms a fenestrate sheet, the width of which is ...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1046/j.1469-7580.2003.00197.x
更新日期：2003-07-01 00:00:00
abstract：:Scales of snakes contain hard proteins (beta-keratins), now referred to as keratin-associated beta-proteins. In the present study we report the isolation, sequencing, and expression of a new group of these proteins from snake epidermis, designated cysteine-glycine-proline-rich proteins. One deduced protein from expres...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/j.1469-7580.2009.01192.x
更新日期：2010-03-01 00:00:00
abstract：:Providing formative assessment opportunities has been recognised as a significant benefit to student learning. The outcome of any formative assessment should be one that ultimately helps improve student learning through familiarising students with the levels of learning required, informing them about gaps in their lea...
journal_title：Journal of anatomy
pub_type： 杂志文章,评审
doi：10.1111/joa.12117
更新日期：2014-03-01 00:00:00
abstract：:The sheep is a commonly used animal model for human lumbar spine surgery, but only in vitro investigations comparing the human and ovine spine exist. Spinal musculature has previously not been compared between man and sheep. This additional knowledge could further indicate to what extent these species are biomechanica...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.12354
更新日期：2015-10-01 00:00:00
abstract：:Primates have shoulders adapted to a wide range of locomotor functions from terrestrial pronograde quadrupedalism to highly arboreal suspensory behaviours. The shape of the scapula tightly follows these functional differences. Previous analyses of primate postcrania, including the scapula, indicate that quadrupedal mo...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/j.1469-7580.2006.00639.x
更新日期：2006-11-01 00:00:00
abstract：:Fresh specimens of human peritoneum collected from heart-beating cadaver organ donors have been examined by transmission electron microscopy. Samples were taken from the anterior abdominal wall and from the surfaces of the liver, stomach and diaphragm. The mesothelium consisted of a single layer of flattened cells gen...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1989-12-01 00:00:00
abstract：:This study set out to determine the pattern of development and distribution of the interstitial cells of Cajal (ICC) in the intestinal tract of the equine fetus and neonate. Intestinal tissue samples from 12 naturally aborted equine fetuses and three euthanized neonates were collected and fixed in formalin prior to ap...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/j.0021-8782.2004.00315.x
更新日期：2004-07-01 00:00:00
abstract：:Mucins are high molecular weight glycoproteins which constitute the major component of the mucus layer and are produce by many epithelial tissues in vertebrates. Osteopontin (OPN) is an adhesive phosphorylated glycoprotein that is expressed by a broad range of tissues and cells. Although gastric mucins MUC1, MUC5AC, M...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/j.1469-7580.2010.01243.x
更新日期：2010-07-01 00:00:00
abstract：:The aortic plexus is a network of sympathetic nerves positioned along the infrarenal abdominal aorta. Recently, we characterized the aortic plexus and its ganglia (inferior mesenteric, left/right spermatic, and prehypogastric ganglion) in males; however, the literature minimally describes its anatomy in females. In th...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.12436
更新日期：2016-05-01 00:00:00
abstract：:It is well known that large pyroninophilic cell proliferation in regional lymphoid tissue preceds and accompanies cell-mediated immunological reactions. In the present study of lymph node responses in the pregnant rat it was found that there were no significant differences between large pyronionphilic cell counts from...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1975-07-01 00:00:00
abstract：:In rats of 40, 49 and 59 days of age the positions of the femoral and tibial nutrient foramina were determined by direct measurement, using a travelling microscope. The femoral nutrient foramen remained constant in position with increasing age, whereas the tibial nutrient foramen moved relatively nearer to the distal ...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1978-03-01 00:00:00
abstract：:The study of human anatomy can be found throughout the rich history of Persia. For thousands of years, morphological descriptions derived from this part of the world have contributed to and have helped form our current anatomical knowledge base. In this article we review the major influential Persian periods and the i...
journal_title：Journal of anatomy
pub_type： 历史文章,杂志文章
doi：10.1111/j.1469-7580.2007.00711.x
更新日期：2007-04-01 00:00:00
abstract：:Continuous tooth replacement is common for tetrapods, but some groups of acrodont lepidosaurs have lost the ability to replace their dentition (monophyodonty). Acrodonty, where the tooth attaches to the apex of the jawbone, is an unusual form of tooth attachment that has been associated with the highly autapomorphic c...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.12754
更新日期：2018-03-01 00:00:00
abstract：:Histochemical fibre types classified in sections stained for succinic dehydrogenase (sdh) and myosin ATPase at pH 9.4, were found to be distributed in a consistent manner within the extensor digitorum longus (EDL) and 4 soleus muscles of the adult rat. Simple morphometric techniques applied to complete transverse sect...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1977-04-01 00:00:00
abstract：:In the intestine, changes of sugar concentration generated in the lumen during digestion induce adaptive responses of glucose transporters in the epithelium. A close matching between the intestinal expression of glucose transporters and the composition and amount of the diet has been provided by several experiments. F...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/j.1469-7580.2011.01385.x
更新日期：2011-08-01 00:00:00
abstract：:The effects of a chronic administration of angiotensin II on the zona fasciculata of rats treated with dexamethasone and maintenance doses of ACTH were investigated by morphometric methods applied to electron microscopy. It was found that angiotensin induced a significant increase in the volume of cells, nuclei, mitoc...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1981-03-01 00:00:00
abstract：:Immunohistochemistry of normal eccrine sweat glands was performed on paraffin sections of human skin. Immunoreactivity (ir) for neuron specific enolase, S100 protein (S100), regulatory peptides, nitric oxide synthase type I (NOS-I) and choline-acetyltransferase (ChAT) was found in small nerve bundles close to sweat gl...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1046/j.1469-7580.1999.19430433.x
更新日期：1999-04-01 00:00:00
abstract：:Fluorescence microscopy and transmission electron microscopy were used to study peribronchial ganglia of cats. An osmiophilic substance that is selectively taken up into the synaptic vesicles of adrenergic nerves (5-hydroxydopamine) was used to help differentiate adrenergic and non-adrenergic terminals. Peribronchial ...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1980-10-01 00:00:00
abstract：:The proposal that the cytoplasmic granules of glomerular peripolar cells (PPCs) of the adult sheep and lamb contain a kallikrein-like substance has been re-examined. After extensive characterisation and purification of anti-kallikrein antibodies, it was concluded that PPCs do not contain immunohistochemically detectab...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1989-02-01 00:00:00
abstract：:This study describes the degenerative changes of the axon terminals making synaptic contacts with the neurons in the submucous ganglia of guinea pig small and large intestines following left or right cervical vagotomy. There were no noticeable ultrastructural changes 1 d after the operation. Beginning at the 3rd posto...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1995-12-01 00:00:00
abstract：:The mammalian cochlea is a remarkable sensory organ, capable of perceiving sound over a range of 10(12) in pressure, and discriminating both infrasonic and ultrasonic frequencies in different species. The sensory hair cells of the mammalian cochlea are exquisitely sensitive, responding to atomic-level deflections at s...
journal_title：Journal of anatomy
pub_type： 杂志文章,评审
doi：10.1111/joa.12314
更新日期：2016-02-01 00:00:00
