Abstract：
BACKGROUND:Wheat protein and its derivatives can cause protein contact dermatitis (PCD), which mainly occurs in bakers. Few studies have attempted to identify the allergens responsible for wheat PCD. OBJECTIVES:The aim of this study was to identify allergenic wheat proteins in patients with wheat PCD. METHODS:Water-soluble and water-insoluble wheat flour proteins were separated by 1- or 2-dimensional gel electrophoresis. IgE-binding proteins were detected by immunoblotting with sera from 3 wheat PCD patients and identified by N-terminal amino acid sequence analysis. The IgE-binding proteins were recombinantly expressed in Escherichia coli and tested against patients' sera. RESULTS:IgE antibodies from the patients' sera reacted with water-soluble proteins rather than water-insoluble proteins, and the 2-dimensional electrophoresis and immunoblotting produced individual IgE-binding patterns. Analysis of the N-terminal amino acid sequence of the IgE-binding proteins from the 2-dimensional gel led to the identification of three glycoproteins, wheat 27-kDa allergen, peroxidase, and purple acid phosphatase. No specific IgE antibodies to their non-glycosylated recombinant proteins were observed. CONCLUSIONS:We identified wheat 27-kDa allergen, peroxidase and purple acid phosphatase as candidate allergens for wheat PCD. Our results suggest that glycan moieties in these proteins are involved in IgE binding.
journal_name
Contact Dermatitisjournal_title
Contact dermatitisauthors
Matsuo H,Uemura M,Yorozuya M,Adachi A,Morita Edoi
10.1111/j.1600-0536.2010.01741.xsubject
Has Abstractpub_date
2010-07-01 00:00:00pages
23-30issue
1eissn
0105-1873issn
1600-0536pii
COD1741journal_volume
63pub_type
杂志文章abstract：:A patient was found to have a delayed positive patch test to hydrocortisone on multiple testing. This proved to be due to a photoallergy with an action spectrum in the UV-A range. The patient coincidently had a polymorphous light eruption in the UV-B range. ...
journal_title：Contact dermatitis
pub_type： 杂志文章
doi：10.1111/j.1600-0536.1978.tb03845.x
更新日期：1978-12-01 00:00:00
abstract：BACKGROUND:The former 'EU Nickel Directive' and, since 2009, the REACH Regulation (item 27 of Annex XVII) do not include all metallic objects. The nickel content of tools is not regulated by the REACH Regulation, even if they may come into in prolonged contact with the skin. Tools might be possible sources of nickel an...
journal_title：Contact dermatitis
pub_type： 杂志文章
doi：10.1111/cod.12462
更新日期：2015-11-01 00:00:00
abstract：BACKGROUND:Protein contact dermatitis is a frequent disorder among hand eczema patients who have occupational food contact. Knowledge about the consequences of having protein contact dermatitis is lacking. OBJECTIVES:To investigate the consequences of having occupational skin disease on the hands resulting from food h...
journal_title：Contact dermatitis
pub_type： 杂志文章
doi：10.1111/j.1600-0536.2012.02101.x
更新日期：2012-12-01 00:00:00
abstract：:Cyclosporine (CSA) is an effective immunosuppressive agent and is used in tissue transplantation. The present investigation was undertaken to determine the effect of topical delivery of CSA on allergic contact dermatitis in guinea pigs. Topical 15% CSA in an azone (1-dodecylazacycloheptan-2-one)-containing vehicle blo...
journal_title：Contact dermatitis
pub_type： 杂志文章
doi：10.1111/j.1600-0536.1989.tb03088.x
更新日期：1989-01-01 00:00:00
abstract：:It has been known since the 1940s that nail polishes contain allergenic ingredients. The aim of this study was to clarify whether the nail polishes on the market today contain significant amounts of allergens, and what the solvents are. The following ingredients were determined: toluene, toluene sulfonamide formaldehy...
journal_title：Contact dermatitis
pub_type： 杂志文章
doi：10.1111/j.1600-0536.1997.tb00189.x
更新日期：1997-10-01 00:00:00
abstract：:Nickel release from Danish one krone coins and metal buttons from jeans has been measured at 20 degrees C in distilled water and at 35 degrees C in distilled water and synthetic sweat. The temperature elevation increased the nickel release from the coins and two of the nine metal buttons investigated. Obviously the se...
journal_title：Contact dermatitis
pub_type： 杂志文章
doi：10.1111/j.1600-0536.1979.tb04803.x
更新日期：1979-03-01 00:00:00
abstract：:The case of a 40-year-old woman with severe edema of the face and neck after the injection of a local dental anesthetic is presented. The reaction is attributed to the presence of sodium metabisulfite, and antioxidant, in the local anesthetic. Both the anesthetic and the sodium metabisulfite gave a delayed positive pa...
journal_title：Contact dermatitis
pub_type： 杂志文章
doi：10.1111/j.1600-0536.1989.tb03120.x
更新日期：1989-02-01 00:00:00
abstract：:Several factors may be responsible for stomatitis in general and the burning mouth syndrome in particular. The results of patch testing are reported in 4 patients with burning mouth symptoms thought to be due to sensitization to denture material. 2 patients reacted to substances in Luxene dentures, but the allergens w...
journal_title：Contact dermatitis
pub_type： 杂志文章
doi：10.1111/j.1600-0536.1988.tb02748.x
更新日期：1988-02-01 00:00:00
abstract：:Among patients consecutively patch tested at St John's from 1984-1989, the prevalence rate of formaldehyde sensitivity was 2.2% for men and 3.7% for women. During these 6 years, 117 women were primarily sensitized by formaldehyde, of whom 61 (52%) had hand eczema. Exposure was occupational in 12% and domestic in 88%. ...
journal_title：Contact dermatitis
pub_type： 杂志文章
doi：10.1111/j.1600-0536.1991.tb01874.x
更新日期：1991-11-01 00:00:00
abstract：:The laser Doppler perfusion scanning technique is an objective, non-invasive assessment method that may be used to assess patch tests. The purpose of this paper is to focus on its clinical use in individuals with light skin, tested with non-pigmented test materials. A laser Doppler perfusion imager PIM 1.0 (LDPI) is u...
journal_title：Contact dermatitis
pub_type： 杂志文章
doi：10.1111/j.1600-0536.1999.tb06059.x
更新日期：1999-05-01 00:00:00
abstract：:Bullous dermatitis, which resolved leaving hyperpigmentation and which was clinically consistent with phytophotodermatitis , affected the skin of the neck of 2 individuals who wore Hawaiian leis (as neck garlands ) made of the fruits of Pelea anisata . In a Daniels culture plate system portions of the fruits showed ph...
journal_title：Contact dermatitis
pub_type： 杂志文章
doi：10.1111/j.1600-0536.1984.tb00104.x
更新日期：1984-04-01 00:00:00
abstract：:Skin compatibility of anionic surfactants may often but not always be predicted by in vitro tests. For instance, the correlation between in vivo and in vitro data is classically hampered in the presence of magnesium. This ion is known to interfere with in vitro skin irritation predictive tests based on protein denatur...
journal_title：Contact dermatitis
pub_type： 杂志文章
doi：10.1111/j.1600-0536.1995.tb00445.x
更新日期：1995-07-01 00:00:00
abstract：:Repeated open application tests (ROATs) were performed with common ingredients of vehicles in 86 patients with contact dermatitis. The substances were applied twice daily for 7 days to the flexor aspect of the forearm near the cubital fossa, unless dermatitis appeared earlier. Of the patients with a questionable (?+) ...
journal_title：Contact dermatitis
pub_type： 杂志文章
doi：10.1111/j.1600-0536.1986.tb01229.x
更新日期：1986-04-01 00:00:00
abstract：:Erythema caused by occlusion of 3 concentrations of surfactant was quantified in vivo using a modified spectrophotometer. Standard chamber irritation test sites were evaluated objectively using the spectrophotometer and subjectively by trained observers. There was a good relationship between the instrumental and visua...
journal_title：Contact dermatitis
pub_type： 杂志文章
doi：10.1111/j.1600-0536.1988.tb02893.x
更新日期：1988-09-01 00:00:00
abstract：:Contact urticaria to potato was confirmed by skin testing in a 26-year-old male with atopic dermatitis, birch-pollen rhinoconjunctivitis and a history of immediate finger itching upon handling raw potato. The potato peel was non-reactive. The urticarial reactivity to potato could be transferred by the patient's serum ...
journal_title：Contact dermatitis
pub_type： 杂志文章
doi：10.1111/j.1600-0536.1983.tb04315.x
更新日期：1983-03-01 00:00:00
abstract：:Tween 80 is an ethoxylated hydrophilic non-ionic surfactant, which is used for the preparation of oil-in-water emulsions in pharmaceutical products, cosmetics, and industrial detergents. Ethoxylated surfactants are polyethers and are thus easily oxidized by atmospheric oxygen to a variety of hydroperoxides, peroxides ...
journal_title：Contact dermatitis
pub_type： 杂志文章
doi：10.1111/j.1600-0536.1997.tb00368.x
更新日期：1997-07-01 00:00:00
abstract：:Predictive guinea pig sensitization studies have been performed with commercial and purified 1H-benzotriazole. In only 3 animals, induced and challenged according to the maximization protocol with more than 100 times the normal use concentration, were slight erythemas elicited with the commercial grade of benzotriazol...
journal_title：Contact dermatitis
pub_type： 杂志文章
doi：10.1111/j.1600-0536.1984.tb00024.x
更新日期：1984-03-01 00:00:00
abstract：:The phenomenon of 'sensitive skin' is a relatively recent complaint in which certain individuals report more intense and frequent adverse sensory effects than the normal population upon use of cosmetic (personal-care) products. Originally defined as a minority complaint, sensitive skin is now claimed by a majority of ...
journal_title：Contact dermatitis
pub_type： 杂志文章,评审
doi：10.1111/j.1600-0536.2009.01697.x
更新日期：2010-03-01 00:00:00
abstract：:In some patients with atopic dermatitis (AD), delayed-type allergy to aeroallergens, such as house dust mite, can be demonstrated by patch testing. Positive patch tests can be expected only in patients with AD and, in them, only to aeroallergens to which type 1 reactions have also been demonstrated. In an as yet unkno...
journal_title：Contact dermatitis
pub_type： 杂志文章,评审
doi：10.1111/j.1600-0536.1989.tb03197.x
更新日期：1989-10-01 00:00:00
abstract：:Henna (Lawsonia inermis, family Lythraceae) is a shrub cultivated in India, Sri Lanka and North Africa and contains the active dye lawsone (2-hydroxy-1,4-naphthoquinone). Henna dye is obtained from the dried leaves, which are powdered and mixed with oil or water and are used to prepare hair and body dyes. Temporary he...
journal_title：Contact dermatitis
pub_type： 杂志文章
doi：10.1111/j.0105-1873.2006.00845.x
更新日期：2006-07-01 00:00:00
abstract：BACKGROUND:An inverse relationship between disease severity and health-related quality of life (HR-QoL) in patients with hand eczema is well established, but modifying effects of demographic variables have been less well studied. OBJECTIVE:To identify the influence of metropolitan versus non-metropolitan residence on ...
journal_title：Contact dermatitis
pub_type： 杂志文章
doi：10.1111/cod.12962
更新日期：2018-05-01 00:00:00
abstract：:Skin protection measures - barrier creams, protective gloves - and skin care are widely recommended for the prevention of occupational hand dermatitis (HD) in skin risk professions, but there is hardly anything known about uptake levels of the measures. The objective of this controlled intervention study was to quanti...
journal_title：Contact dermatitis
pub_type： 杂志文章
doi：10.1034/j.1600-0536.2002.460204.x
更新日期：2002-02-01 00:00:00
abstract：:Bibliographic sources for the complex subject of aroma chemicals are reviewed. The references are not comprehensive but are those which we found to be of value in finding out some of the opportunities for contact with a sensitizing chemical compound, cinnamic aldehyde. ...
journal_title：Contact dermatitis
pub_type： 杂志文章
doi：10.1111/j.1600-0536.1975.tb05310.x
更新日期：1975-01-01 00:00:00
abstract：:This report reviews individual-related variables, environment-related variables and instrument-related variables, with a focus on the Evaporimeter EP1 (ServoMed). Start-up and use is described, and guidelines for good laboratory practice given. ...
journal_title：Contact dermatitis
pub_type： 杂志文章
doi：10.1111/j.1600-0536.1990.tb01553.x
更新日期：1990-03-01 00:00:00
abstract：:The purpose of this study was to investigate the role of contact hypersensitivity (CHS) in adult widespread eczema (WE) and to analyse the aetiology of WE. 108 consecutive adult WE patients were patch tested. 352 patients with suspected localized allergic contact dermatitis (ACD) tested in the same period served as a ...
journal_title：Contact dermatitis
pub_type： 杂志文章
doi：10.1034/j.1600-0536.2002.470605.x
更新日期：2002-12-01 00:00:00
abstract：BACKGROUND:Only a few epidemiological studies on hand eczema (HE) in the metalworking industry have been conducted, and no study has attempted a long-term follow-up. OBJECTIVES:In the Prospective Audi Cohort (PACO) II follow-up study, we aimed to estimate burden and prognosis of HE in a car industry setting (follow-up...
journal_title：Contact dermatitis
pub_type： 杂志文章
doi：10.1111/j.1600-0536.2008.01329.x
更新日期：2008-06-01 00:00:00
abstract：BACKGROUND:The preservative methyldibromo glutaronitrile (MDBGN) has been banned, first from stay-on, and later from rinse-off cosmetics, in the EU countries because of increasing rates of contact allergy. OBJECTIVES:To evaluate the frequency of contact allergy to MDBGN among patients patch tested by the Danish Contac...
journal_title：Contact dermatitis
pub_type： 杂志文章
doi：10.1111/j.1600-0536.2008.01364.x
更新日期：2008-07-01 00:00:00
abstract：:Thirty-six of 37 veterinary surgeons with incapacitating dermatitis had allergic contact dermatitis, mainly from antibiotics such as spiramycin, Penethamate BP and tylosin. Eight were sensitive to rubber materials. Twenty of 36 had positive prick or scratch tests, mainly to animal hair. The atopic sensitivities were r...
journal_title：Contact dermatitis
pub_type： 杂志文章
doi：10.1111/j.1600-0536.1980.tb03885.x
更新日期：1980-01-01 00:00:00
abstract：:16 children with atopic dermatitis and 4 nonatopics were skin tested with flavourings and perfumes. Immediate reactions to balsam of Peru and fragrance-mix were found in 9 atopics, and none among nonatopics. An irritant is more probable than an immunologic mechanism. Allergen solutions should probably be assayed at a ...
journal_title：Contact dermatitis
pub_type： 杂志文章
doi：10.1111/j.1600-0536.1992.tb05196.x
更新日期：1992-07-01 00:00:00
abstract：:Serious adverse skin reactions to permanent hair dyes and temporary black tattoos have been reported. As temporary tattoos have become fashionable among adolescents, the risk profile for p-phenylenediamine (PPD) sensitization of the population has changed simultaneously with an increasing use of hair dyes in this age ...
journal_title：Contact dermatitis
pub_type： 杂志文章,多中心研究
doi：10.1111/j.0105-1873.2006.00746.x
更新日期：2006-02-01 00:00:00
