Butyrate stimulates IL-32alpha expression in human intestinal epithelial cell lines.

Abstract:

AIM:To investigate the effects of butyrate on interleukin (IL)-32alpha expression in epithelial cell lines. METHODS:The human intestinal epithelial cell lines HT-29, SW480, and T84 were used. Intracellular IL-32alpha was determined by Western blotting analyses. IL-32alpha mRNA expression was analyzed by real-time polymerase chain reaction. RESULTS:Acetate and propionate had no effects on IL-32alpha mRNA expression. Butyrate significantly enhanced IL-32alpha expression in all cell lines. Butyrate also up-regulated IL-1beta-induced IL-32alpha mRNA expression. Butyrate did not modulate the activation of phosphatidylinositol 3-kinase (PI3K), a mediator of IL-32alpha expression. Like butyrate, trichostatin A, a histone deacetylase inhibitor, also enhanced IL-1beta-induced IL-32alpha mRNA expression. CONCLUSION:Butyrate stimulated IL-32alpha expression in epithelial cell lines. An epigenetic mechanism, such as histone hyperacetylation, might be involved in the action of butyrate on IL-32alpha expression.

journal_name

World J Gastroenterol

authors

Kobori A,Bamba S,Imaeda H,Ban H,Tsujikawa T,Saito Y,Fujiyama Y,Andoh A

doi

10.3748/wjg.v16.i19.2355

subject

Has Abstract

pub_date

2010-05-21 00:00:00

pages

2355-61

issue

19

eissn

1007-9327

issn

2219-2840

journal_volume

16

pub_type

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