Abstract:
AIM:To evaluate the best diagnostic technique and risk factors of the human Cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) infection in inflammatory bowel disease (IBD). METHODS:A cohort of 40 IBD patients (17 refractory) and 40 controls underwent peripheral blood and endoscopic colonic mucosal sample harvest. Viral infection was assessed by quantitative real-time polymerase chain reaction and immunohistochemistry, and correlations with clinical and endoscopic indexes of activity, and risk factors were investigated. RESULTS:All refractory patients carried detectable levels of HCMV and/or EBV mucosal load as compared to 13/23 (56.5%) non-refractory and 13/40 (32.5%) controls. The median DNA value was significantly higher in refractory (HCMV 286 and EBV 5.440 copies/10(5) cells) than in non-refractory (HCMV 0 and EBV 6 copies/10(5) cells; P < 0.05 and < 0.001) IBD patients and controls (HCMV and EBV 0 copies/10(5) cells; P < 0.001 for both). Refractory patients showed DNA peak values ≥ 10(3) copies/10(5) cells in diseased mucosa in comparison to non-diseased mucosa (P < 0.0121 for HCMV and < 0.0004 for EBV), while non-refractory patients and controls invariably displayed levels below this threshold, thus allowing us to differentiate viral colitis from mucosal infection. Moreover, the mucosal load positively correlated with the values found in the peripheral blood, whilst no correlation with the number of positive cells at immunohistochemistry was found. Steroid use was identified as a significant risk factor for both HCMV (P = 0.018) and EBV (P = 0.002) colitis. Finally, a course of specific antiviral therapy with ganciclovir was successful in all refractory patients with HCMV colitis, whilst refractory patients with EBV colitis did not show any improvement despite steroid tapering and discontinuation of the other medications. CONCLUSION:Viral colitis appeared to contribute to mucosal lesions in refractory IBD, and its correct diagnosis and management require quantitative real-time polymerase chain reaction assay of mucosal specimens.
journal_name
World J Gastroenteroljournal_title
World journal of gastroenterologyauthors
Ciccocioppo R,Racca F,Paolucci S,Campanini G,Pozzi L,Betti E,Riboni R,Vanoli A,Baldanti F,Corazza GRdoi
10.3748/wjg.v21.i6.1915subject
Has Abstractpub_date
2015-02-14 00:00:00pages
1915-26issue
6eissn
1007-9327issn
2219-2840journal_volume
21pub_type
杂志文章abstract:AIM:To sum up the clinical characteristics of patients with central pontine myelinolysis (CPM) after orthotopic liver transplantation (OLT) and to document the possible causes of CPM. METHODS:Data of 142 patients undergoing OLT between January 1999 to May 2003 were analyzed retrospectively. Following risk factors duri...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v10.i17.2540
更新日期:2004-09-01 00:00:00
abstract:AIM:To investigate whether ANNs and LDA could recognize patients with ABG in a database, containing only clinical and biochemical variables, of a pool of patients with and without ABG, by selecting the most predictive variables and by reducing input data to the minimum. METHODS:Data was collected from 350 consecutive ...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v11.i37.5867
更新日期:2005-10-07 00:00:00
abstract::A 67-year-old male underwent endoscopic submucosal dissection (ESD) to treat early gastric cancer (EGC) in 2001. The lesion (50 mm × 25 mm diameter) was histologically diagnosed as poorly differentiated adenocarcinoma, with an ulcer finding. Although the tumor was confined to the mucosa with no evidence of lymphovascu...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v19.i30.5016
更新日期:2013-08-14 00:00:00
abstract:AIM:To evaluate the effect of sitagliptin vs placebo on histologic and non-histologic parameters of non-alcoholic steatohepatitis (NASH). METHODS:Twelve patients with biopsy-proven NASH were randomized to sitagliptin (100 mg daily) (n = 6) or placebo (n = 6) for 24 wk. The primary outcome was improvement in liver fibr...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,随机对照试验
doi:10.3748/wjg.v23.i1.141
更新日期:2017-01-07 00:00:00
abstract::Ulcerative colitis (UC) is a chronic disease characterized by diffuse inflammation of the mucosa of the colon and rectum. The hallmark clinical symptom of UC is bloody diarrhea. The clinical course is marked by exacerbations and remissions, which may occur spontaneously or in response to treatment changes or intercurr...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v20.i28.9458
更新日期:2014-07-28 00:00:00
abstract::Benign multicystic peritoneal mesothelioma (BMPM) is a rare cystic mesothelial lesion that occurs predominantly in reproductive aged women. A 56-year-old Caucasian male was admitted to our surgical department with a chief complaint of a painful mass in his right lower abdomen for almost 2 years. The physical examinati...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v19.i39.6689
更新日期:2013-10-21 00:00:00
abstract:AIM:To analyze RASSF6 expression in pancreatic ductal adenocarcinoma (PDAC) and to determine whether RASSF6 has an independent prognostic value in PDAC. METHODS:We studied RASSF6 expression in 96 histologically confirmed PDAC samples and 20 chronic pancreatitis specimens using immunohistochemistry and real-time quanti...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v21.i21.6621
更新日期:2015-06-07 00:00:00
abstract:AIM:To evaluate the value of ABC (D) stratification [combination of serum pepsinogen and Helicobacter pylori (H. pylori) antibody] of patients with gastric cancer. METHODS:Ninety-five consecutive patients with gastric cancer were enrolled into the study. The serum pepsinogen I (PG I)/pepsinogen II (PG II) and H. pylor...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v17.i43.4793
更新日期:2011-11-21 00:00:00
abstract:AIM:A single-chain antibody fragment, ND-1scFv, against human colorectal carcinoma was constructed and expressed in E.coli, and its biodistribution and pharmacokinetic properties were studied in mice bearing tumor. METHODS:V(H) and V(L) genes were amplified from hybridoma cell IC-2, secreting monoclonal antibody ND-1,...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v9.i4.726
更新日期:2003-04-01 00:00:00
abstract::Pancreatic cancer is a leading cause of cancer mortality and the incidence of this disease is expected to continue increasing. While patients with pancreatic cancer have traditionally faced a dismal prognosis, over the past several years various advances in diagnosis and treatment have begun to positively impact this ...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v20.i28.9354
更新日期:2014-07-28 00:00:00
abstract:BACKGROUND:Progressive familial intrahepatic cholestasis (PFIC) encompasses a group of autosomal recessive disorders with high morbidity and mortality. Variants in the gene encoding tight junction protein-2 (TJP2) have been linked to PFIC type 4 (PFIC4), which predominantly presents in childhood. However, there are onl...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v26.i5.550
更新日期:2020-02-07 00:00:00
abstract::Hepatocellular carcinoma (HCC) is one of the 10 most common cancers worldwide. There is no ideal treatment for HCC yet and many researchers are trying to improve the effects of treatment by changing therapeutic strategies. As the majority of human cancers seem to exhibit either abnormal p53 gene or disrupted p53 gene ...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v13.i7.985
更新日期:2007-02-21 00:00:00
abstract::In this review, we discuss recent advances in nucleic acid-based therapeutic technologies that target hepatitis C virus (HCV) infection. Because the HCV genome is present exclusively in RNA form during replication, various nucleic acid-based therapeutic approaches targeting the HCV genome, such as ribozymes, aptamers,...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v19.i47.8949
更新日期:2013-12-21 00:00:00
abstract:AIM:To identify the prevalence of hepatitis B e antigen (HBeAg) and to assess the association of hepatitis B virus (HBV) core promoter mutations and viral load in Indonesian patients. METHODS:Sixty-four patients with chronic hepatitis, 65 with liver cirrhosis and 50 with hepatocellular carcinoma were included in this ...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v17.i6.708
更新日期:2011-02-14 00:00:00
abstract::Hepatitis C virus (HCV) affects 130-210 million people worldwide and is one of the major risk factors for hepatocellular carcinoma. Globally, at least one third of hepatocellular carcinoma cases are attributed to HCV infection, and 350000 people died from HCV related diseases per year. There is a great geographical va...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v20.i28.9270
更新日期:2014-07-28 00:00:00
abstract::It is reported that a pancreatic disease may precede the diagnosis of inflammatory bowel disease (IBD) both in children and in adults. Idiopathic chronic pancreatitis, however, occasionally co-exists with the IBD, mainly at pediatric age. We report a case of a patient who progressively developed the features of a chro...
journal_title:World journal of gastroenterology
pub_type: 信件
doi:10.3748/wjg.v19.i31.5204
更新日期:2013-08-21 00:00:00
abstract:AIM:To assess the risk of relapse in ulcerative colitis (UC) patients in clinical remission using mucosal status and fecal immunochemical test (FIT) results. METHODS:The clinical outcomes of 194 UC patients in clinical remission who underwent colonoscopy were based on evaluations of Mayo endoscopic subscores (MESs) an...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v22.i21.5079
更新日期:2016-06-07 00:00:00
abstract:AIM:To study the role of gastric mucosal ascorbic acid (AA) in the progression of acute gastric mucosal lesions induced by compound 48/80 (C48/80), a mast cell degranulator, in rats. METHODS:C48/80 (0.75 mg/kg) was intraperitoneally injected to fasted Wistar rats. Oral administration of AA (10, 50 or 100 mg/kg) was pe...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v11.i9.1324
更新日期:2005-03-07 00:00:00
abstract:AIM:To investigate the effects of guggulsterone on the proliferation and apoptosis of human hepatoma HepG2 cells in vitro and relevant mechanisms. METHODS:Human hepatocellular carcinoma HepG2 cells and normal human liver L-02 cells were treated with different concentrations of guggulsterone (5-100 μmol/L) for 24-72 h....
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v21.i47.13277
更新日期:2015-12-21 00:00:00
abstract::Epidemiologic studies have suggested a relation between hepatitis C virus (HCV) infection and diabetes mellitus. HCV infection is emerging as a metabolic disease, and diabetes mellitus as a risk factor for HCV infection. However, some data on the prevalence of antibodies to HCV in patients with diabetes are conflictin...
journal_title:World journal of gastroenterology
pub_type: 评论,信件
doi:10.3748/wjg.15.5114
更新日期:2009-10-28 00:00:00
abstract:AIM:To evaluate the safety of lamivudine (LAM) treatment for chronic hepatitis B in early pregnancy. METHODS:A total of 92 pregnant women who received LAM treatment either before pregnancy or in early pregnancy were enrolled in this study. All of the pregnant women volunteered to take lamivudine during pregnancy and w...
journal_title:World journal of gastroenterology
pub_type: 临床试验,杂志文章
doi:10.3748/wjg.v18.i45.6645
更新日期:2012-12-07 00:00:00
abstract::Despite progress in the treatment of advanced and metastatic pancreatic cancer (PC), the outcome of this disease remains dismal for the majority of patients. Given the moderate efficacy of treatment, prognostic factors may help to guide treatment decisions. Several trials identified baseline performance status as an i...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v13.i2.224
更新日期:2007-01-14 00:00:00
abstract:AIM:To investigate the value of ultrasound elastography combined with serological indexes in diagnosing liver fibrosis and assessing its severity. METHODS:A total of 338 chronic hepatitis B (CHB) patients were divided into a disease group (patients with hepatic fibrosis) and control group (subjects without hepatic fib...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v24.i37.4272
更新日期:2018-10-07 00:00:00
abstract::A case of pedunculated Brunner's gland hamartoma (BGH) of the duodenum causing upper gastrointestinal (GI) hemorrhage is reported. The patient was a 47-year-old man who visited our hospital for further evaluation of tarry stools and shortness of breath. Endoscopic examination of the upper digestive tract revealed a la...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.15.373
更新日期:2009-01-21 00:00:00
abstract::We report a case of pancreatic hemolymphangioma. Hemolymphangioma is a malformation of both lymphatic vessels and blood vessels. The incidence of this disease in the pancreas is extremely rare. To the best of our knowledge, only seven cases have been reported worldwide (PubMed). A 39-year-old woman with a one-day hist...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v19.i22.3520
更新日期:2013-06-14 00:00:00
abstract:AIM:To investigate in patients with functional dyspepsia (FD) after an every-day meal whether (1) gastrointestinal (GI) and extra-GI symptoms had any relation with the degree of antral volume, (2) the onset of postprandial symptoms was associated with, and may predict, delayed gastric emptying. METHODS:In 94 symptomat...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v12.i43.6982
更新日期:2006-11-21 00:00:00
abstract::Eosinophilic gastroenteritis (EG) is an uncommon disease characterized by focal or diffuse eosinophilic infiltration of the gastrointestinal tract, and is usually associated with dyspepsia, diarrhea and peripheral eosinophilia. Diffuse gastrointestinal tract and colonic involvement are uncommon. The endoscopic appeara...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.15.2156
更新日期:2009-05-07 00:00:00
abstract::Casais et al have reported an inverse correlation between serum phosphate and body weight after administration of sodium phosphate at a dose of 60 g. Our group has already described the relationship between body weight and hyperphosphatemia with these preparations, although our study was not quoted by Casais. We perfo...
journal_title:World journal of gastroenterology
pub_type: 评论,信件
doi:10.3748/wjg.v17.i21.2681
更新日期:2011-06-07 00:00:00
abstract:AIM:To explore the grade and stage of pathology and the relationship between grading and staging of hepatic fibrosis and noninvasive diagnostic parameters. METHODS:Inflammatory activity and fibrosis of consecutive liver biopsies from 200 patients with chronic liver disease were determined according to the Diagnostic C...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v9.i11.2574
更新日期:2003-11-01 00:00:00
abstract:AIM:To establish the safety and efficacy of an indigenously developed r-hepatitis B vaccine using an accelerated schedule and to highlight the social awareness and commitment in preventing the spreading of hepatitis B virus infection. METHODS:The study was a multicentric, double blind, randomized (3:1) study using thr...
journal_title:World journal of gastroenterology
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.3748/wjg.v11.i7.1037
更新日期:2005-02-21 00:00:00