Abstract:
:Agilent GC-MS MSD Chemstation offers automated library search report for toxicological screening using total ion chromatogram (TIC) and mass spectroscopy in normal mode. Numerous peaks appear in the chromatogram of biological specimen such as blood or urine and often large migrating peaks obscure small target peaks, in addition, any target peaks of low abundance regularly give wrong library search result or low matching score. As a result, retention time and mass spectrum of all the peaks in the chromatogram have to be checked to see if they are relevant. These repeated actions are very tedious and time-consuming to toxicologists. MSD Chemstation software operates using a number of macro files which give commands and instructions on how to work on and extract data from the chromatogram and spectroscopy. These macro files are developed by the own compiler of the software. All the original macro files can be modified and new macro files can be added to the original software by users. To get more accurate results with more convenient method and to save time for data analysis, we developed new macro files for reports generation and inserted new menus in the Enhanced Data Analysis program. Toxicological screening reports generated by these new macro files are in text mode or graphic mode and these reports can be generated with three different automated subtraction options. Text reports have Brief mode and Full mode and graphic reports have the option with or without mass spectrum mode. Matched mass spectrum and matching score for detected compounds are printed in reports by modified library searching modules. We have also developed an independent application program named DrugMan. This program manages drug groups, lists and parameters that are in use in MSD Chemstation. The incorporation of DrugMan with modified macro modules provides a powerful tool for toxicological screening and save a lot of valuable time on toxicological work.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Choe S,Kim S,Choi H,Choi H,Chung H,Hwang Bdoi
10.1016/j.forsciint.2010.03.003subject
Has Abstractpub_date
2010-06-15 00:00:00pages
50-7issue
1-3eissn
0379-0738issn
1872-6283pii
S0379-0738(10)00095-2journal_volume
199pub_type
杂志文章abstract::Fungi colonizing cadavers are capable of drug metabolism and may thus change the metabolite pattern or concentration of drugs in forensic postmortem samples. The purpose of this study was to check for the presence of such changes by searching fungi-specific metabolites of four model drugs (amitriptyline, metoprolol, m...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2016.03.006
更新日期:2016-05-01 00:00:00
abstract::The aim of the present study was to investigate stress gene expression in cultured primary fibroblasts established from Achilles tendons collected during autopsies from sudden infant death syndrome (SIDS) cases, and age-matched controls (infants dying in a traumatic event). Expression of 4 stress responsive genes, HSP...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2013.06.003
更新日期:2013-10-10 00:00:00
abstract::A new solid-phase extraction procedure for the determination of cocaine and some of its metabolites in brain tissue, using high-flow co-polymeric sorbents is reported as a substantial improvement on our recently reported procedure. The recovery of cocaine, norcocaine and cocaethylene was excellent as was the reproduci...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(92)90197-5
更新日期:1992-03-01 00:00:00
abstract::Forensic pathologists are familiar with alcohol abusers, who are found dead and in whom the cause of death cannot be ascertained. In order to examine the possible role of ketoacidosis for the cause of death in this group of alcohol abusers, the concentrations of ketone bodies (acetone, acetoacetate, D-beta-hydroxybuty...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(95)01783-6
更新日期:1995-10-30 00:00:00
abstract::The identification and the quantitative estimation of cannabis constituents is important for forensic purposes. High resolution gas-chromatography gives better results than gas-liquid chromatography with packed columns, as it shows a better resolution and higher number of constituents. Different quantitative values we...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(83)90101-9
更新日期:1983-03-01 00:00:00
abstract::Allele- and genotype frequencies of the two short tandem repeat (STR) systems VWA and TH01 were determined in an Austrian population sample by polymerase chain reaction (PCR) analysis. A total of 9 alleles for VWA and 6 alleles for TH01 could be observed in a population of 278 (VWA) and 276 (TH01) individuals. Both sy...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(97)00053-4
更新日期:1997-06-23 00:00:00
abstract::A duplex primer set for the amplification of mitochondrial DNA HVI and HVII control regions was evaluated for the optimization of a DNA sequencing protocol suitable for forensic casework. HVI and HVII products, with the absence of non-specific products, could be detected by agarose gel electrophoresis when as little a...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2004.09.128
更新日期:2005-11-25 00:00:00
abstract::The causes of sudden cardiac death are diverse and are function of age. In young people, coronary anomalies, hypertrophic cardiomyopathy are the most common findings at autopsies; in adults, coronary atherosclerosis and acquired forms of cardiomyopathy are frequent findings. In many of non-ischemia related cases autop...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2004.09.011
更新日期:2004-12-02 00:00:00
abstract::A high-performance liquid chromatography-tandem mass spectrometry (LC/MS/MS) technique was developed for the simultaneous determination of five non-steroidal anti-inflammatory oxicam drugs (ampiroxicam, tenoxicam, piroxicam, meloxicam and lornoxicam) in human plasma. These five oxicam drugs and isoxicam (internal stan...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2012.11.016
更新日期:2013-04-10 00:00:00
abstract::Reported are various techniques to determine the early postmortem period by examining the eye. These include corneal opacity, retinal vessel segmentation, pupil reaction, retinal changes and intraocular pressure. All are subjective, requiring experience to implement the techniques. ...
journal_title:Forensic science international
pub_type: 杂志文章,评审
doi:10.1016/0379-0738(94)90230-5
更新日期:1994-02-01 00:00:00
abstract::We have identified a new compound in capsules marketed as plant feeders available from internet suppliers. It is apparent from internet forums that these so-called plant feeders are being used as recreational drugs. The material is identified as being 3'-fluoromethcathinone. The compound in the capsule was identified ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2008.11.013
更新日期:2009-03-10 00:00:00
abstract::The exposition to heavy metal-rich airborne due to fire practicing has forced to the development of heavy metal-free environmental ammunition primers all over the world. Here we characterize the GSR elements present in the Brazilian lead-free ammunition produced by Companhia Brasileira de Cartuchos (CBC) and commercia...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2007.07.005
更新日期:2008-05-02 00:00:00
abstract::The hairy maggot blow fly, Chrysomya rufifacies (Diptera: Calliphoridae), is a forensically important fly often encountered on human and other vertebrate remains in temperate and tropic regions throughout the world including Australia, Asia, Central America and North America. C. rufifacies was reared under controlled ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2014.09.023
更新日期:2014-12-01 00:00:00
abstract::The article presents research on the methods of crash velocity determination based on the Compact car class. The database used in the research is provided by the NHTSA (National Highway Traffic Safety Administration) and includes numerous frontal crash tests, which allowed the determination of the mathematical model p...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2018.11.023
更新日期:2019-02-01 00:00:00
abstract::Establishing a person's identity can be a very difficult process. Dental, fingerprint and DNA comparisons are probably the most common techniques used in this context, allowing fast and secure identification processes. However, since they cannot always be used, sometimes it is necessary to apply different and less kno...
journal_title:Forensic science international
pub_type: 杂志文章,评审
doi:10.1016/j.forsciint.2006.04.010
更新日期:2007-01-05 00:00:00
abstract:BACKGROUND:We report two cases of drug-facilitated sexual assault which presented with CNS depression rendering the victims vulnerable to sexual assault and unable to recall the events surrounding the crimes. In each tetrahydrozoline (THZ) was quantified. CASE REPORT:Case #1 ingested an unknown amount of eye drops con...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2012.04.004
更新日期:2012-09-10 00:00:00
abstract::A 76-year-old male with a previous history of heart arrhythmia and decompensation was treated for a complicated thoraco-abdominal trauma. Accidentally, a pleural drain was inserted in his heart through the ventricular septum, perforating the left ventricle, the drain tip being buried in its posterior wall. This injury...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(81)90186-9
更新日期:1981-01-01 00:00:00
abstract::The increasing use of the potent hallucinogenic drug D-lysergic acid diethylamide (LSD) makes the application of highly sensitive analytical techniques necessary. For an effective analysis of biological specimens a limit of detection in the sub-ng/ml range is required. Using capillary electrophoresis (CE), with laser-...
journal_title:Forensic science international
pub_type: 杂志文章,评审
doi:10.1016/s0379-0738(98)00017-6
更新日期:1998-04-05 00:00:00
abstract::Suicide attempts can be described to include all actions taken by an individual to end their life as a result of acute desperation. In parallel with the changes in the make-up of human societies both globally and in this country in recent years, suicide attempts are receiving attention in ever increasing proportions. ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(00)00237-1
更新日期:2000-09-11 00:00:00
abstract:AIM:To validate a method for determining equivalent drug cutoff concentrations for tetrahydrocannabinol and amphetamine in blood and oral fluid, which ensures that the drug prevalence in samples of blood and oral fluid taken simultaneously is equal. METHODS:A method using regression analysis of drug concentrations for...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2011.06.024
更新日期:2011-10-10 00:00:00
abstract::A partial DNA sequence of cytochrome b gene was used to identify the remains of endangered animals and species endemic to Taiwan. The conservation of animals species included in this study were: the formosan gem-faced civets, leopard cats, tigers, clouded leopards, lion, formosan muntjacs, formosan sika deers, formosa...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(01)00403-0
更新日期:2001-10-15 00:00:00
abstract::Fatalities due to an extreme ambient temperature might present with poor or nonspecific pathologies; thus, the diagnosis of the cause of death in such cases is one of the most difficult tasks in forensic pathology. The present study investigated the molecular pathology of alveolar damage involving pulmonary edema with...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2013.03.007
更新日期:2013-05-10 00:00:00
abstract::Biochemical characterization in alveolar lavage fluids of rats which had inhaled 5% oxygen, carbon dioxide and carbon monoxide was studied in comparison with control rats. The protein content, consisting mainly of serum albumin, markedly increased in the hypoxic states. The phospholipid content also increased one and ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(89)90088-1
更新日期:1989-08-01 00:00:00
abstract::The authors describe a technique for the gross examination of postmortem eyes of children who are suspected to have been the victims of deliberate trauma. By removing the anterior segment (cornea, iris, lens and pars plicata of the ciliary body) en bloc by a coronal incision through the pars plana just anterior to the...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(92)90149-q
更新日期:1992-09-01 00:00:00
abstract::This is the first report in the forensic literature of a combination of fatal digoxin poisoning with endocardial fibroelastosis (EFE). Typical morphological features of EFE as the cause of clinically diagnosed cardiomyopathy were present in the autopsy of a 3-year-old girl, including cardiac hypertrophy and marked thi...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(97)00134-5
更新日期:1997-10-06 00:00:00
abstract:AIMS:The aim of this work is to test the stability of mephedrone added to whole blood collected from alive and dead mephedrone free-users and stored at three different temperatures (-20, +4 and +20°C) with and without preservatives up to 6 months, trying to establish the best storage condition in order to reduce possib...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2015.07.021
更新日期:2015-11-01 00:00:00
abstract::In the field, the muscid flies Muscina stabulans (Fallén) and Muscina prolapsa (Harris) only colonised buried baits in June, July and August. The two-species co-occurred on baits buried at 5cm but only M. prolapsa colonised baits buried at 10cm. Other species of insect were seldom recovered from buried baits regardles...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2016.06.013
更新日期:2016-09-01 00:00:00
abstract::The main challenge for the identification and differentiation of lipsticks for forensic purposes is the fact that they have a similar chemical composition - in particular, samples of the same hue. The objective of the presented research was to solve this problem using a nondestructive analytical technique - attenuated...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2017.09.019
更新日期:2017-11-01 00:00:00
abstract::Stature estimation is a standard procedure in the fields of forensic and biological anthropology, bio-archaeology and paleoanthropology, in order to gain biological insights into the individuals/populations studied. The most accurate stature estimation method is based on anatomical reconstruction (i.e., the Fully meth...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2013.01.009
更新日期:2013-03-10 00:00:00
abstract::A new series of fingerprint developing membrane were prepared using ninhydrin as the developing agent, and pressure-sensitive emulsifiers as the encapsulated chemicals. The type of emulsifier, plastic film, concentration of the developing agent, modifying ions and thickness of the membrane were studied in order to get...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2014.06.036
更新日期:2014-09-01 00:00:00