Abstract:
:A partial DNA sequence of cytochrome b gene was used to identify the remains of endangered animals and species endemic to Taiwan. The conservation of animals species included in this study were: the formosan gem-faced civets, leopard cats, tigers, clouded leopards, lion, formosan muntjacs, formosan sika deers, formosan sambars, formosan serows, water buffalo, formosan pangolins and formosan macaques. The control species used included domestic cats, domestic dogs, domestic sheeps, domestic cattles, domestic pigs and humans. Heteroplasmy was detected in the formosan macaque, domestic pig and domestic cats. The frequencies of heteroplasmy in these animals were about 0.25% (1 in 402bp). Sequences were aligned by Pileup program of GCG computer package, and the phylogenetic tree was constructed by the neighbor-joining method. The results of sequence comparison showed that the percentage range of sequence diversity in the same species was from 0.25 to 2.74%, and that between the different species was from 5.97 to 34.83%. The results of phylogenetic analysis showed that the genetic distance between the different species was from 6.33 to 40.59. Animals of the same species, both the endangered animal species and domestic animals, were clustered together in the neighbor-joining tree. Three unknown samples of animal remains were identified by this system. The partial sequence of cytochrome b gene adopted in this study proved to be usable for animal identification.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Hsieh HM,Chiang HL,Tsai LC,Lai SY,Huang NE,Linacre A,Lee JCdoi
10.1016/s0379-0738(01)00403-0keywords:
subject
Has Abstractpub_date
2001-10-15 00:00:00pages
7-18issue
1eissn
0379-0738issn
1872-6283pii
S0379-0738(01)00403-0journal_volume
122pub_type
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abstract::Around 27,000 people were killed in the province of Asturias during the Spanish Civil War, with several thousands killed after the war ended. There are currently over 2,000 known mass burial locations throughout Spain, but many more are unknown. Geophysics is a useful tool employed to help in the active attempts to do...
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pub_type: 历史文章,杂志文章
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2018.08.019
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journal_title:Forensic science international
pub_type: 杂志文章
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更新日期:1999-11-08 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
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doi:10.1016/j.forsciint.2005.02.010
更新日期:2005-08-11 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.forsciint.2014.10.029
更新日期:2014-12-01 00:00:00
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journal_title:Forensic science international
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journal_title:Forensic science international
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