当前位置： SCI文献检索 > EUROPEAN JOURNAL OF PHARMACOLOGY期刊下所有文献 > Stimulation of the T-cell receptors CD3 and CD2 with OKT3 and OKT11 antibodies activates a common pertussis toxin-insensitive G-protein.

Stimulation of the T-cell receptors CD3 and CD2 with OKT3 and OKT11 antibodies activates a common pertussis toxin-insensitive G-protein.

Abstract：

:The association of G-proteins with the T-cell-specific receptor structures CD3 and CD2 was investigated. High-affinity GTPase activity in membrane preparations of the human leukemic T-cell line Jurkat could be induced by the monoclonal antibodies OKT3 (anti-CD3) and OKT11 (anti-CD2). When combining maximally active concentrations of OKT3 and OKT11, no additive effect was seen on GTPase activity. In mutant Jurkat cells lacking the CD3 complex but with an intact CD2 receptor, neither OKT3 nor OKT11 could stimulate GTPase activity. Activation of CD3 and CD2 by monoclonal antibodies also stimulated phospholipase C activity as measured by breakdown of membrane phosphoinositides in wild-type but not in mutant Jurkat cells. Neither GTPase nor phospholipase C activation was sensitive to pretreatment with doses of pertussis toxin (PTX) that caused ADP ribosylation of a sensitive G-protein. Our data show that the CD3 complex and the CD2 receptor may activate a common PTX-insensitive G-protein. The CD2 receptor appears to stimulate the G-protein by interacting with the CD3 complex. The data are compatible with, but do not prove, that this G-protein is involved in the activation of phospholipase C by the two receptors.

journal_name

Eur J Pharmacol

journal_title

European journal of pharmacology

authors

Kvanta A,Gerwins P,van der Ploeg I,Nordstedt C,Jondal M,Fredholm BB

doi

10.1016/0922-4106(90)90033-t

subject

Has Abstract

pub_date

1990-12-15 00:00:00

pages

363-72

issue

6

eissn

0014-2999

issn

1879-0712

journal_volume

189

pub_type

杂志文章

相关文献

EUROPEAN JOURNAL OF PHARMACOLOGY文献大全
  • The sigma receptor ligand rimcazole alters secretion of prolactin and alpha-melanocyte stimulating hormone by dopaminergic and non-dopaminergic mechanisms.

    abstract：:The role of tuberoinfundibular and periventricular-hypophysial dopaminergic neurons in mediating rimcazole-induced decreases in plasma concentrations of prolactin and alpha-melanocyte stimulating hormone was assessed. Dopaminergic neuronal activity was estimated by measuring concentrations of 3,4-dihydroxyphenylacetic...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(95)00841-1

    authors： Eaton MJ,Lookingland KJ,Moore KE

    更新日期：1996-03-28 00:00:00

  • Glucocorticoids induce endothelin release from vascular smooth muscle cells but not endothelial cells.

    abstract：:Vascular smooth muscle cells in culture are capable of secreting endothelin which is a vasoconstrictor and mitogenic peptide. The effect of glucocorticoids on endothelin release from vascular smooth muscle cells of the rat and rabbit aortas was investigated. Micromolar concentrations of dexamethasone and cortisol caus...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(91)90641-3

    authors： Kanse SM,Takahashi K,Warren JB,Ghatei M,Bloom SR

    更新日期：1991-06-18 00:00:00

  • KDR-5169, a new gastrointestinal prokinetic agent, enhances gastric contractile and emptying activities in dogs and rats.

    abstract：:KDR-5169, 4-amino-5-chloro-N-[1-(3-fluoro-4-methoxybenzyl)piperidin-4-yl]-2-(2-hydroxyethoxy)benzamide hydrochloride dihydrate, is a new prokinetic with a dual action, i.e., stimulation of the 5-HT4 receptor and antagonism of the dopamine D2 receptor. In this study, we determined in vitro activities of KDR-5169 toward...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0014-2999(01)01543-6

    authors： Tazawa S,Masuda N,Koizumi T,Kitazawa M,Nakane T,Miyata H

    更新日期：2002-01-11 00:00:00

  • Tissue-specific localization of mitochondrial imidazoline-guanidinium receptive sites.

    abstract：:In the present report, we studied the distribution of the imidazoline-guanidinium receptive site in mitochondrial fractions from different rabbit and human tissues. Binding studies of the imidazoline-guanidinium receptive site ligand [3H]idazoxan, allowed to distinguish two groups of tissues: the first one, including ...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(92)90316-v

    authors： Tesson F,Limon I,Parini A

    更新日期：1992-08-25 00:00:00

  • Epinephrine as a potent releaser of immunoreactive beta-endorphin in rats.

    abstract：:The intravenous infusion of catecholamines to rats induced a dose dependent increase of immunoreactive beta-endorphin (beta-Endi) in plasma. The ED50 values of 1-epinephrine and 1-isoproterenol were 110 and 100 ng/kg X min respectively. 1-Propranolol, but not d-propranolol prevented the effect of 1-epinephrine. Infusi...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(81)90303-4

    authors： Berkenbosch F,Vermes I,Tilders FJ

    更新日期：1981-06-10 00:00:00

  • Novel arylaminopyridazine-GABA receptor antagonists examined electrophysiologically in Ascaris suum.

    abstract：:The structure-activity relationships of 35 novel derivatives of 2-(carboxypropyl)-3-amino-4-methyl-6-phenyl pyridazine (SR 95103) were examined as gamma-aminobutyric acid (GABA) antagonists in the flap preparation of the parasitic nematode, Ascaris suum, using a two-microelectrode current-clamp technique. All but one ...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(94)00778-6

    authors： Martin RJ,Sitamze JM,Duittoz AH,Wermuth CG

    更新日期：1995-03-24 00:00:00

  • The 5-HT1-like receptor mediating reduction of porcine carotid arteriovenous shunting by RU 24969 is not related to either the 5-HT1A or the 5-HT1B subtype.

    abstract：:Using the radioactive microsphere technique in anaesthetized pigs, we studied the systemic and carotid haemodynamic effects of intracarotid infusions (0.3, 1, 3 and 10 micrograms/kg.min) of 5-methoxy-3-(1,2,3,6-tetrahydro-4-pyridinyl)-1H-indole succinate (RU 24969), a drug with high affinity for 5-HT1A and 5-HT1B reco...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(89)90432-9

    authors： Bom AH,Villalón CM,Verdouw PD,Saxena PR

    更新日期：1989-11-14 00:00:00

  • Pharmacological characterisation of postjunctional 5-HT receptors in cerebral arteries from the sheep.

    abstract：:In the sheep cerebral vasculature 5-hydroxytryptamine (5-HT) caused a contraction of which ketanserin was found to be an effective antagonist (Basilar artery, pA2 = 9.33 +/- 0.16; middle cerebral artery, pA2 = 9.19 +/- 0.16; pial artery, pA2 = 9.47 +/- 0.12). Sumatriptan (GR 43175), a selective 5-HT1-like receptor ago...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(90)90399-q

    authors： Gaw AJ,Wadsworth RM,Humphrey PP

    更新日期：1990-04-10 00:00:00

  • Endothelin ET(A) receptor antagonist reverses the inhibitory effect of platelet-derived growth factor on cytokine-induced nitric oxide production.

    abstract：:Cytokines and cytokine-induced nitric oxide (NO) play important roles in inflammatory glomerular diseases, and both platelet-derived growth factor and transforming growth factor-beta inhibit cytokine-induced NO production. In this study, we demonstrated that a selective endothelin ET(A) receptor antagonist, BQ-485 (He...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0014-2999(98)00849-8

    authors： Hirahashi J,Nakaki T,Hishikawa K,Marumo T,Hayashi M,Saruta T

    更新日期：1999-01-15 00:00:00

  • Acetylsalicylic acid inhibits α,β-meATP-induced facilitation of neck muscle nociception in mice--implications for acute treatment of tension-type headache.

    abstract：:Infusion of α,β-methylene ATP (α,β-meATP) into murine neck muscle facilitates brainstem nociception. This animal experimental model is suggested to be appropriate for investigating pathophysiological mechanisms in tension-type headache. It was hypothesized that d-lysine acetylsalicylic acid (ASA, aspirin®) reverses th...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2011.10.008

    authors： Ristic D,Spangenberg P,Ellrich J

    更新日期：2011-12-30 00:00:00

  • PCA 50941, a novel Ca2+ channel agonist.

    abstract：:PCA 50941 is a novel 1,4-dihydropyridine derivative. Its vasoconstricting effects prompted a systematic comparison with the prototypic Ca2+ channel activator, Bay K 8644. The two compounds exhibit marked analogies and differences in their cardiovascular profiles. PCA 50941 exhibits a pronounced vascular over cardiac s...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(93)90163-c

    authors： Priego J,González-Morales MA,Cillero FJ,Villarroya M,Sunkel C,de Casa-Juana MF,López MG,Artalejo CR,de Pascual R,García AG

    更新日期：1993-10-12 00:00:00

  • Stimulation of chondrogenesis in ATDC5 chondroprogenitor cells and hypertrophy in mouse by Genkwadaphnin.

    abstract：:The growth in height of the bone plate is a result of endochondral proliferation in epiphyseal growth plates and the conversion of chondrocytes into new bone. The control of chondrogenic differentiation and hypertrophy is critical for these processes. The present study was aimed to demonstrate the chondromodulating ac...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2011.01.012

    authors： Choi HJ,Nepal M,Park YR,Lee HK,Oh SR,Soh Y

    更新日期：2011-03-25 00:00:00

  • Capsaicin-induced desensitization is prevented by capsazepine but not by ruthenium red in guinea pig bronchi.

    abstract：:In isolated guinea pig bronchi, the influence of ruthenium red, capsazepine and extracellular Ca2+ on capsaicin-induced desensitization was examined to investigate whether this desensitization was mediated via a specific receptor coupled with an ion channel. Pre-exposure of tissues to capsaicin (1, 3 or 10 microM) cau...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0014-2999(98)00786-9

    authors： Jung YS,Cho TS,Moon CH,Shin HS

    更新日期：1998-12-04 00:00:00

  • Action of calcitonin gene-related peptide on rat aortic smooth muscle.

    abstract：:The effects of rat calcitonin gene-related peptide (CGRP) on superfused rat aortic smooth muscle cells in cell culture were investigated. Exposure of the cells for 10 min to CGRP (10(-7) M), with or without pretreatment with pertussis toxin, stimulated the release of cyclic AMP but not of prostacyclin, as judged by ra...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(89)90062-9

    authors： Vallotton MB,Dolci W,Gerber-Wicht C,Fischer JA

    更新日期：1989-07-18 00:00:00

  • Effects of KC 2450 on the lower esophageal sphincter in vivo and in vitro.

    abstract：:The effect of KC 2450 (racemic 3,5-cis-3-methylamino-2,3,4,5-tetrahydro-1-benzoxepine-5-ol hydrochloride) on lower esophageal sphincter pressure in pentobarbital-anesthetized dogs was determined and compared to the effect of metoclopramide. The ED20 value (i.e. the dose that increased lower esophageal sphincter pressu...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(87)90111-7

    authors： Fowler PJ,Nelson AH,Price WJ,Sarau HM,Grous M,Lombardi DM,Barone FC,Ormsbee HS 3rd

    更新日期：1987-10-13 00:00:00

  • Inhibition of nucleoside uptake in human erythrocytes by a new series of compounds related to lidoflazine and mioflazine.

    abstract：:The zero-trans influx of uridine in human erythrocytes is inhibited by lidoflazine and analogs thereof. The concentrations required for inhibition of nucleoside transport were higher when the compounds were simultaneously added with uridine than upon preincubation of the inhibitors with the erythrocytes. R70380 proved...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0922-4106(90)90040-5

    authors： Pirovano IM,Van Belle H,Ijzerman AP

    更新日期：1990-12-15 00:00:00

  • Effect of nifedipine on cyclosporine A-induced nephrotoxicity, urinary endothelin excretion and renal endothelin receptor number.

    abstract：:The aim of the present study was to determine the effect of a calcium channel blocker on renal function, urinary endothelin excretion and endothelin receptor number in rats. Administration of cyclosporine resulted in a significant impairment of renal function when measured by either [14C]inulin or 24 h creatinine clea...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(91)90132-a

    authors： Brooks DP,Ohlstein EH,Contino LC,Storer B,Pullen M,Caltabiano M,Nambi P

    更新日期：1991-02-26 00:00:00

  • Myocardial and pulmonary uptake of S-1'-[18F]fluorocarazolol in intact rats reflects radioligand binding to beta-adrenoceptors.

    abstract：:The biodistribution of S-(-)-4-(2-hydroxy-3-(1'-[18F]fluoroisopropyl)- aminopropoxy)carbazole ([18F]S-fluorocarazolol, a non-selective beta-adrenoceptor antagonist) was studied in rats (60 min after 18F injection when specific binding in peripheral organs was maximal). 18F uptake in brain, erythrocytes, heart and lung...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(94)00636-l

    authors： Van Waarde A,Elsinga PH,Brodde OE,Visser GM,Vaalburg W

    更新日期：1995-01-16 00:00:00

  • Inhibitory effect of spinal mGlu(5) receptor antisense oligonucleotide on the up-regulated expression of spinal G protein associated with chronic morphine treatment.

    abstract：:Knockdown of spinal metabotropic glutamate 5 (mGlu5) receptor was shown to inhibit the development of intrathecal morphine antinociceptive tolerance. The present work was designed to evaluate the expression of spinal G-protein during morphine tolerance and knockdown of spinal mGlu5 receptor with antisense oligonucleot...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2013.11.024

    authors： Chen M,Zhang X,Xu H,Ma X,Jiang W,Xu T

    更新日期：2014-01-15 00:00:00

  • Mitomycin C induces fibroblasts apoptosis and reduces epidural fibrosis by regulating miR-200b and its targeting of RhoE.

    abstract：:Mitomycin C (MMC) is known to reduce epidural fibrosis, but the underlying mechanisms have not yet been elucidated. Aberrant miR-200b expressions have been reported in multiple types of fibrotic tissues from many diseases. The aim of this study was to clarify the mechanism by which MMC induces fibroblasts apoptosis an...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2015.08.002

    authors： Sun Y,Ge Y,Fu Y,Yan L,Cai J,Shi K,Cao X,Lu C

    更新日期：2015-10-15 00:00:00

  • Effects of THA on ionic currents in myelinated axons of Xenopus laevis.

    abstract：:In voltage-clamp experiments with the myelinated nerve fibre of Xenopus laevis, 9-amino-1,2,3,4-tetrahydroacridine (THA) decreased both Na+ and K+ currents and shifted the steady state inactivation potential curve in a negative direction. The effects may be described as (a) a decrease of the permeability constant PNa,...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(89)90272-0

    authors： Elinder F,Mohammed AK,Winblad B,Arhem P

    更新日期：1989-05-30 00:00:00

  • (-)-Trans-epsilon-viniferin, a polyphenol present in wines, is an inhibitor of noradrenaline and 5-hydroxytryptamine uptake and of monoamine oxidase activity.

    abstract：:(-)-Trans-epsilon-viniferin (epsilon-viniferin, 5-200 microM), a dimer of resveratrol, concentration-dependently inhibited the uptake of [3H]noradrenaline and [3H]5-HT by synaptosomes from rat brain (being slightly but significantly more selective against [3H]noradrenaline) and the uptake of [3H]5-HT by human platelet...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2006.06.005

    authors： Yáñez M,Fraiz N,Cano E,Orallo F

    更新日期：2006-08-07 00:00:00

  • GABA modulates the release of dopamine and acetylcholine from rat caudate nucleus slices.

    abstract：:The effects of GABA on depolarization-induced (26 mM K+) release of radiolabeled dopamine (DA) and acetylcholine (ACh) from slices of rat caudate nucleus were examined with a superfusion method. GABA, in concentrations of 10(-5)--10(-3) M, dose-dependently enhanced the release of DA, either accumulated by high-affinit...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(79)90101-8

    authors： Stoof JC,Den Breejen EJ,Mulder AH

    更新日期：1979-07-15 00:00:00

  • Cyclic AMP increase in canine parotid gland mediated by histamine H2-receptors.

    abstract：:Histamine, 4-methylhistamine and dimaprit induced a dose-dependent increase in the cyclic AMP content of chopped canine parotid gland in the presence of 3-isobutyl-1-methylxanthine. Metiamide, but not mepyramine, inhibited the effect of these agonists dose-dependently. The cyclic AMP content was also increased by 2-(2...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(81)90101-1

    authors： Nishibori M

    更新日期：1981-12-17 00:00:00

  • Mouse models rarely mimic the transcriptome of human neurodegenerative diseases: A systematic bioinformatics-based critique of preclinical models.

    abstract：:Translational research for neurodegenerative disease depends intimately upon animal models. Unfortunately, promising therapies developed using mouse models mostly fail in clinical trials, highlighting uncertainty about how well mouse models mimic human neurodegenerative disease at the molecular level. We compared the ...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2015.03.021

    authors： Burns TC,Li MD,Mehta S,Awad AJ,Morgan AA

    更新日期：2015-07-15 00:00:00

  • Ryanodine-induced contraction in femoral artery from spontaneously hypertensive rats.

    abstract：:The mechanisms of ryanodine-induced contractions were studied in strips of femoral arteries from spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto rats (WKY). Ryanodine (30 nM to 30 microM) alone contracted arterial strips in a dose-dependent manner. The maximum contraction in SHR was about 5 times g...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(94)90383-2

    authors： Kojima M,Dohi Y,Sato K

    更新日期：1994-03-11 00:00:00

  • Submaxillary secretion in rats treated with guanethidine.

    abstract：:The composition of final submaxillary saliva and of whole gland homogenates were compared in rats treated with 14 daily doses of guanethidine (20 mg/kg body weight) and in untreated controls after stimulation with pilocarpine (10 mg/kg) or carbamylcholine (50-100 mg/kg). A 44% reduction in the volume of saliva and ele...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/0014-2999(77)90200-x

    authors： Eccher S,Dodson C,Quissell DO,Martinez JR

    更新日期：1977-01-21 00:00:00

  • Isobolographic analysis of interactions between 1-methyl-1,2,3,4-tetrahydroisoquinoline and four conventional antiepileptic drugs in the mouse maximal electroshock-induced seizure model.

    abstract：:The anticonvulsant activity of 1-methyl-1,2,3,4-tetrahydroisoquinoline (MeTHIQ--an endogenous parkinsonism-preventing substance) administered alone and in combination with four conventional antiepileptic drugs (carbamazepine, phenytoin, phenobarbital and valproate) was determined in the mouse maximal electroshock-indu...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2008.11.049

    authors： Luszczki JJ,Antkiewicz-Michaluk L,Czuczwar SJ

    更新日期：2009-01-14 00:00:00

  • Clomipramine block of the hERG K+ channel: accessibility to F656 and Y652.

    abstract：:Clomipramine is a tricyclic antidepressant for psychiatric disorders that can induce QT prolongation, which may lead to torsades de pointes. Since blockade of cardiac human ether-a-go-go-related gene (hERG) channels is an important cause of acquired long QT syndrome, we investigated the acute effects of clomipramine o...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.ejphar.2008.06.094

    authors： Jo SH,Hong HK,Chong SH,Won KH,Jung SJ,Choe H

    更新日期：2008-09-11 00:00:00

  • PPARalpha and PPARdelta activators inhibit cytokine-induced nuclear translocation of NF-kappaB and expression of VCAM-1 in EAhy926 endothelial cells.

    abstract：:Endothelium injury is a primary event in atherogenesis, which is followed by monocyte infiltration, macrophage differentiation, and smooth muscle cell migration. Peroxisome proliferator-activated receptors (PPARs) are transcription factors now recognized as important mediators in the inflammatory response. The aim of ...

    journal_title：European journal of pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0014-2999(01)01589-8

    authors： Rival Y,Benéteau N,Taillandier T,Pezet M,Dupont-Passelaigue E,Patoiseau JF,Junquéro D,Colpaert FC,Delhon A

    更新日期：2002-01-25 00:00:00