Abstract：
:A number of aptamers have been selected against cell surface biomarkers or against eukaryotic tissue culture cells themselves. To determine the general utility of aptamers for assessing the cell surface proteome, we developed a standardized flow cytometry assay and carried out a comprehensive study with 7 different aptamers and 14 different cell lines. By examining how aptamers performed with a variety of cell lines, we identified difficulties in using aptamers for cell typing. While there are some aptamers that show excellent correlation between cell surface binding and the expression of a biomarker on the cell surface, other aptamers showed nonspecific binding by flow cytometry. For example, it has recently been claimed that an anti-PTK7 (protein tyrosine kinase 7) aptamer identified a new biomarker for leukemia cells, but data with the additional cell lines shows that it is possible that the aptamer instead identifies a propensity for adherence. Better understanding and controlling for the role of background and nonspecific binding to cells should open the way to using arrays of aptamers for describing and quantifying the cell surface proteome.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Li N,Ebright JN,Stovall GM,Chen X,Nguyen HH,Singh A,Syrett A,Ellington ADdoi
10.1021/pr801048zsubject
Has Abstractpub_date
2009-05-01 00:00:00pages
2438-48issue
5eissn
1535-3893issn
1535-3907journal_volume
8pub_type
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更新日期：2011-07-01 00:00:00
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更新日期：2009-08-01 00:00:00
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journal_title：Journal of proteome research
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更新日期：2015-01-02 00:00:00
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