Abstract：
:The ability of concentrated formic acid to formylate reactive amino acid residues is known from previous reports. In contrast, solvents containing a low concentration of formic acid are generally recognized as a safe environment for proteomic applications. The primary objective of this study was to explain the excessive formylation rate in tryptic peptides that did not come into contact with concentrated formic acid. We found out that the peptide formylation was associated with dissolving the peptides in a solvent containing mere 0.1% formic acid. Similar conclusions were drawn after analyzing publicly available proteomic data. We further demonstrated that these unwanted modifications can be averted via handling the samples at a low temperature or, obviously, via replacing formic acid in the solvent with trifluoroacetic acid. These simple countermeasures can contribute to a reduction in the part of the MS/MS spectra that remain unassigned to a peptide sequence.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Lenčo J,Khalikova MA,Švec Fdoi
10.1021/acs.jproteome.9b00823subject
Has Abstractpub_date
2020-03-06 00:00:00pages
993-999issue
3eissn
1535-3893issn
1535-3907journal_volume
19pub_type
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