Identification of Alzheimer's Disease Autoantibodies and Their Target Biomarkers by Phage Microarrays.

abstract：:Reversible phosphorylations play a critical role in most biological pathways. Hence, in signaling studies great effort has been put into identification of a maximum number of phosphosites per experiment. Mass spectrometry (MS)-based phosphoproteomics approaches have been proven to be an ideal analytical method for map...

journal_title：Journal of proteome research

authors： Zarei M,Sprenger A,Metzger F,Gretzmeier C,Dengjel J

更新日期：2011-08-05 00:00:00

abstract：:Mass spectrometry is being used to identify protein biomarkers that can facilitate development of drug treatment. Mass spectrometry-based labeling proteomic experiments result in complex proteomic data that is hierarchical in nature often with small sample size studies. The generalized linear model (GLM) is the most p...

journal_title：Journal of proteome research

authors： D'Angelo G,Chaerkady R,Yu W,Hizal DB,Hess S,Zhao W,Lekstrom K,Guo X,White WI,Roskos L,Bowen MA,Yang H

更新日期：2017-09-01 00:00:00

abstract：:The effects of acute and chronic stress on the production of systemic metabolites were investigated in male Sprague-Dawley (SD) rats. Metabolites excreted in urine were analyzed using GC/MS in conjunction with multivariate and univariate statistical techniques. SD rats were subjected to two kinds of acute stress and c...

journal_title：Journal of proteome research

authors： Wang X,Zhao T,Qiu Y,Su M,Jiang T,Zhou M,Zhao A,Jia W

更新日期：2009-05-01 00:00:00

abstract：:Plant secretory (Class III) peroxidases are redox enzymes that rely on N-glycosylation for full enzyme activity and stability. Peroxidases from palm tree leaves comprise the most stable and active plant peroxidases characterized to date. Herein, site-specific glycosylation and microheterogeneity of windmill palm tree ...

journal_title：Journal of proteome research

authors： Baker MR,Tabb DL,Ching T,Zimmerman LJ,Sakharov IY,Li QX

更新日期：2016-06-03 00:00:00

abstract：:The localization of proteins at a tissue- or cell-type-specific level is tightly linked to the protein function. To better understand each protein's role in cellular systems, spatial information constitutes an important complement to quantitative data. The standard methods for determining the spatial distribution of p...

journal_title：Journal of proteome research

authors： Sivertsson Å,Lindström E,Oksvold P,Katona B,Hikmet F,Vuu J,Gustavsson J,Sjöstedt E,von Feilitzen K,Kampf C,Schwenk JM,Uhlén M,Lindskog C

更新日期：2020-12-04 00:00:00

abstract：:Human milk is a rich source of bioactive proteins that support the early growth and development of the newborn. Although the major components of the protein fraction in human milk have been studied, the expression and relative abundance of minor components have received limited attention. We examined the expression of...

journal_title：Journal of proteome research

authors： Liao Y,Alvarado R,Phinney B,Lönnerdal B

更新日期：2011-04-01 00:00:00

abstract：:Reported in this work are the development of mass spectrometric immunoassay (MSIA) devices and methods for the qualitative analysis of IGF-1 and -2, and the rigorous quantification of IGF-1 from human plasma. A method involving addition of SDS in moderate concentration to unfractionated plasma for disrupting IGF/IGFBP...

journal_title：Journal of proteome research

authors： Nelson RW,Nedelkov D,Tubbs KA,Kiernan UA

更新日期：2004-07-01 00:00:00

abstract：:We used a peptidomic approach for the analysis of the low molecular weight proteome in rat pancreatic tissue extracts. The goal was to develop a method that allows identifying endogenous peptides produced in the pancreas in the course of acute pancreatitis. The workflow combines peptides enrichment by centrifugal ultr...

journal_title：Journal of proteome research

authors： Lassout O,Pastor CM,Fétaud-Lapierre V,Hochstrasser DF,Frossard JL,Lescuyer P

更新日期：2010-09-03 00:00:00

abstract：:A new fast method for identification and characterization of proteolytic digests of proteins by monolithic liquid chromatography coupled with mass spectrometry has been developed. The advantages of the monolithic columns are a high-pressure stability and low back pressure resulting in higher flow rates for capillary o...

journal_title：Journal of proteome research

authors： Marcus K,Schäfer H,Klaus S,Bunse C,Swart R,Meyer HE

更新日期：2007-02-01 00:00:00

abstract：:Traditional measures of short-term stress response such as fecal glucocorticoid metabolites (FGM) are widely used in controlled settings to quantify the intensity of stimulation to which cattle are exposed. However, FGMs present several methodological and interpretation pitfalls when applied on animals in free-ranging...

journal_title：Journal of proteome research

authors： Valerio A,Casadei L,Giuliani A,Valerio M

更新日期：2020-02-07 00:00:00

abstract：:Nostoc punctiforme ATCC 29133 is a photoautotrophic cyanobacterium with the capacity to fix atmospheric N 2. Its ability to mediate this process is similar to that described for Nostoc sp. PCC 7120, where vegetative cells differentiate into heterocysts. Quantitative proteomic investigations at both the filament level ...

journal_title：Journal of proteome research

authors： Ow SY,Noirel J,Cardona T,Taton A,Lindblad P,Stensjö K,Wright PC

更新日期：2009-01-01 00:00:00

abstract：:Stroke remains a major public health problem worldwide; it causes severe disability and is associated with high mortality rates. However, early diagnosis of stroke is difficult, and no reliable biomarkers are currently established. In this study, mass-spectrometry-based metabolomics was utilized to characterize the me...

journal_title：Journal of proteome research

authors： Liu P,Li R,Antonov AA,Wang L,Li W,Hua Y,Guo H,Wang L,Liu P,Chen L,Tian Y,Xu F,Zhang Z,Zhu Y,Huang Y

更新日期：2017-02-03 00:00:00

abstract：:Histones regulate a variety of chromatin templated events by their post-translational modifications (PTMs). Although there are extensive reports on the PTMs of canonical histones, the information on the histone variants remains very scanty. Here, we report the identification of different PTMs, such as acetylation, met...

journal_title：Journal of proteome research

authors： Pentakota SK,Sandhya S,P Sikarwar A,Chandra N,Satyanarayana Rao MR

更新日期：2014-12-05 00:00:00

abstract：:Type 1 diabetes, in human patients and NOD mice, results from an immune attack on insulin-producing beta-cells of the pancreas by autoreactive T lymphocytes. In NOD mice, genetically controlled perturbations in the signaling pathways downstream of the antigen-specific T cell receptor (TCR) may be instrumental in the a...

journal_title：Journal of proteome research

authors： Iwai LK,Benoist C,Mathis D,White FM

更新日期：2010-06-04 00:00:00

abstract：:Glycosylation is one of the most common post-translational modifications in proteins, existing in ~50% of mammalian proteins. Several research groups have demonstrated that mass spectrometry is an efficient technique for glycopeptide identification; however, this problem is still challenging because of the enormous di...

journal_title：Journal of proteome research

authors： Zhang Y,Yu CY,Song E,Li SC,Mechref Y,Tang H,Liu X

更新日期：2015-12-04 00:00:00

abstract：:Increased throughput as well as increased multiplexing of liquid chromatography coupled to selected reaction monitoring mass spectrometry (LC-SRM-MS) assays for protein quantification challenges routine data analysis. Despite the measurement of multiple transitions from multiple peptides, for clinical applications a s...

journal_title：Journal of proteome research

authors： van den Broek I,Mastali M,Mouapi K,Bystrom C,Bairey Merz CN,Van Eyk JE

更新日期：2020-06-05 00:00:00

abstract：:The purpose of this study was to evaluate the use of high resolution LC-MS together with metabolomics and D(4)-cholic acid (D(4)-CA) as a metabolic tracer to measure the metabolism and reconjugation of bile acids (BAs) in vitro and in vivo. Metabolic tracers are very important because they allow for the direct detecti...

journal_title：Journal of proteome research

authors： Castro-Perez JM,Roddy TP,Shah V,Wang SP,Ouyang X,Ogawa A,McLaren DG,Tadin-Strapps M,Robinson MJ,Bartz SR,Ason B,Chen Y,Previs SF,Wong KK,Vreeken RJ,Johns DG,Hubbard BK,Hankemeier T,Mitnaul L

更新日期：2011-10-07 00:00:00

abstract：:Cytokinin hormones are crucial regulators of a large number of processes in plant development. Recently, significant progress has been made toward the elucidation of the molecular details of cytokinin that has led to a model for signal transduction involving a phosphorylation cascade. However, the current knowledge of...

journal_title：Journal of proteome research

authors： Heintz D,Erxleben A,High AA,Wurtz V,Reski R,Van Dorsselaer A,Sarnighausen E

更新日期：2006-09-01 00:00:00

abstract：:Mammary tumors are a major health threat to women and female dogs. In both, metastasis of the primary tumor to distant organs is the most common cause of tumor-related death. Nevertheless, the molecular mechanisms of tumor metastasis are far from being understood, and it is still unknown why some human and canine carc...

journal_title：Journal of proteome research

authors： Klopfleisch R,Klose P,Weise C,Bondzio A,Multhaup G,Einspanier R,Gruber AD

更新日期：2010-12-03 00:00:00

abstract：:We developed an ELISA in high-density microarray format to detect hepatocyte growth factor (HGF) in human serum. The microassay can detect HGF at sub-pg/mL concentrations in sample volumes of 100 microL or less. The microassay is also quantitative and was used to detect elevated HGF levels in sera from recurrent breas...

journal_title：Journal of proteome research

authors： Woodbury RL,Varnum SM,Zangar RC

更新日期：2002-05-01 00:00:00

abstract：:New technologies have advanced the field of proteomics, and a number of companies have developed innovative platforms to drive this research. However, significant challenges are often encountered when trying to integrate complementary technologies from multiple manufacturers. We have developed a software and hardware ...

journal_title：Journal of proteome research

authors： Mahnke RC,Corzett TH,McCutchen-Maloney SL,Chromy BA

更新日期：2006-09-01 00:00:00

abstract：:In humans, the olfactory epithelium is located in two narrow passages, the olfactory clefts, at the upper part of the nasal cavities. The olfactory epithelium is covered by a mucus layer which is essential for the function of the olfactory neurons that are directly connected with the brain through the cribriform plate...

journal_title：Journal of proteome research

authors： Débat H,Eloit C,Blon F,Sarazin B,Henry C,Huet JC,Trotier D,Pernollet JC

更新日期：2007-05-01 00:00:00

abstract：:We report the use of chemical derivatization with MALDI-MS/MS analysis for de novo sequence analysis. Using three frequently used homology-based search algorithms, we were able to identify more than 40 proteins from banana, a nonmodel plant with unsequenced genome. Furthermore, this approach allowed the identification...

journal_title：Journal of proteome research

authors： Samyn B,Sergeant K,Carpentier S,Debyser G,Panis B,Swennen R,Van Beeumen J

更新日期：2007-01-01 00:00:00

abstract：:Linear, short-chain polyfluorinated and perfluorinated alkyl compounds, often referred to as PFCs, have been in worldwide use as surfactants and polymer precursors for decades, and environmental dispersal of these highly persistent compounds represents a public health threat. Whereas ubiquitous low-level exposure to t...

journal_title：Journal of proteome research

authors： Hansmeier N,Chao TC,Herbstman JB,Goldman LR,Witter FR,Halden RU

更新日期：2015-01-02 00:00:00

abstract：:"Omics" studies reported to date have dealt with either thoroughly characterized single species or poorly explored meta-microbial communities. However, these techniques are capable of producing highly informative data for the analysis of interactions between two organisms. We examined the bacterial interaction between...

journal_title：Journal of proteome research

authors： Nakanishi Y,Fukuda S,Chikayama E,Kimura Y,Ohno H,Kikuchi J

更新日期：2011-02-04 00:00:00

abstract：:Phytopathogenic fungi are one of the most damaging plant parasitic organisms, and can cause serious diseases and important yield losses in crops. The study of the biology of these microorganisms and the interaction with their hosts has experienced great advances in recent years due to the development of moderm, holist...

journal_title：Journal of proteome research

authors： Gonzalez-Fernandez R,Jorrin-Novo JV

更新日期：2012-01-01 00:00:00

abstract：:Controlled ovarian hyperstimulation is performed to assist with generation of multiple mature oocytes for use in in vitro fertilization (IVF). The goal of our study was to evaluate differences in protein and steroid profiles in ovarian follicular fluid (hFF) samples obtained during oocyte retrieval from women undergoi...

journal_title：Journal of proteome research

authors： Kushnir MM,Naessén T,Wanggren K,Rockwood AL,Crockett DK,Bergquist J

更新日期：2012-10-05 00:00:00

abstract：:The notion of a gamete recognition system that alerts females to the presence of gametes in their reproductive tract profoundly influences our understanding of the physiology of events leading to conception and the bearing of offspring. Here, we show that the female responds to gametes within her tract by modulating t...

journal_title：Journal of proteome research

authors： Georgiou AS,Snijders AP,Sostaric E,Aflatoonian R,Vazquez JL,Vazquez JM,Roca J,Martinez EA,Wright PC,Fazeli A

更新日期：2007-12-01 00:00:00

abstract：:Improvements in proteome analysis of erythrocyte membrane proteins by two-dimensional electrophoresis are here reported. In particular, a differential extraction procedure was set up allowing separation of integral membrane proteins from peripheral species. Moreover, the use of dilute Immobiline gels (down to as low a...

journal_title：Journal of proteome research

authors： Bruschi M,Seppi C,Arena S,Musante L,Santucci L,Balduini C,Scaloni A,Lanciotti M,Righetti PG,Candiano G

更新日期：2005-07-01 00:00:00

abstract：:Protein phosphorylation is the most abundant reversible covalent modification. Human protein kinases participate in almost all biological pathways, and approximately half of the kinases are associated with disease. PhoSigNet was designed to store and display human phosphorylation-mediated signal transduction networks,...

journal_title：Journal of proteome research

authors： Zhang M,Li H,He Y,Sun H,Xia L,Wang L,Sun B,Ma L,Zhang G,Li J,Li Y,Xie L

更新日期：2015-07-02 00:00:00