Abstract：
:Histones regulate a variety of chromatin templated events by their post-translational modifications (PTMs). Although there are extensive reports on the PTMs of canonical histones, the information on the histone variants remains very scanty. Here, we report the identification of different PTMs, such as acetylation, methylation, and phosphorylation of a major mammalian histone variant TH2B. Our mass spectrometric analysis has led to the identification of both conserved and unique modifications across tetraploid spermatocytes and haploid spermatids. We have also computationally derived the 3-dimensional model of a TH2B containing nucleosome in order to study the spatial orientation of the PTMs identified and their effect on nucleosome stability and DNA binding potential. From our nucleosome model, it is evident that substitution of specific amino acid residues in TH2B results in both differential histone-DNA and histone-histone contacts. Furthermore, we have also observed that acetylation on the N-terminal tail of TH2B weakens the interactions with the DNA. These results provide direct evidence that, similar to somatic H2B, the testis specific histone TH2B also undergoes multiple PTMs, suggesting the possibility of chromatin regulation by such covalent modifications in mammalian male germ cells.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Pentakota SK,Sandhya S,P Sikarwar A,Chandra N,Satyanarayana Rao MRdoi
10.1021/pr500597asubject
Has Abstractpub_date
2014-12-05 00:00:00pages
5603-17issue
12eissn
1535-3893issn
1535-3907journal_volume
13pub_type
杂志文章abstract：:We describe Peptoonist, a program that can automatically identify the glycans (sugars) present at each N-glycosylation site of a protein. The input to Peptoonist is a series of mass spectra, both MS and MS/MS, obtained from a liquid chromatography (LC) run of proteolytically digested purified glycoproteins. The progra...
journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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更新日期：2007-02-01 00:00:00
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journal_title：Journal of proteome research
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更新日期：2013-06-07 00:00:00
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journal_title：Journal of proteome research
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更新日期：2018-07-06 00:00:00
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更新日期：2012-04-06 00:00:00
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