Abstract：
:Renal cell carcinoma (RCC) tissue is composed of a mixture of neoplastic and normal cells, which complicate proteome analysis. The aim of our study was to investigate whether it is feasible to establish primary cell cultures of RCC and of renal cortex maintaining the tissue phenotype along with a more homogeneous and enriched cytological material. Fourteen (82.3%) primary cultures from 17 surgical cases were established and characterized by morphology, growth rate, immunocytochemistry, and molecular analysis performed by Real-time PCR, Western blotting, two-dimensional electrophoresis (2-DE), and mass spectrometry. Cultures showed >90% cytokeratine-positive epithelial cells. In primary tumor cultures, the molecular phenotype of manganese superoxide dismutase and heat shock protein 27 was the same as that found in tumor tissues with overexpression and increased number of isoforms. Moreover, 27 out 28 specific proteins and their isoforms, present in spots excised from 2-DE gel of cortex or RCC cultures, corresponded to those identified on the 2-DE tissue cortex reference map, suggesting that these primary cultures retain the proteomic profile of the corresponding tissues.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Perego RA,Bianchi C,Corizzato M,Eroini B,Torsello B,Valsecchi C,Di Fonzo A,Cordani N,Favini P,Ferrero S,Pitto M,Sarto C,Magni F,Rocco F,Mocarelli Pdoi
10.1021/pr050002okeywords:
subject
Has Abstractpub_date
2005-09-01 00:00:00pages
1503-10issue
5eissn
1535-3893issn
1535-3907journal_volume
4pub_type
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journal_title：Journal of proteome research
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