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Phospho-iTRAQ: assessing isobaric labels for the large-scale study of phosphopeptide stoichiometry.

Abstract：

:The ability to distinguish between phosphopeptides of high and low stoichiometry is essential to discover the true extent of protein phosphorylation. We here extend the strategy whereby a peptide sample is briefly split in two identical parts and differentially labeled preceding the phosphatase treatment of one part. Our use of isobaric tags for relative and absolute quantitation (iTRAQ) marks the first time that isobaric tags have been applied for the large-scale analysis of phosphopeptides. Our Phospho-iTRAQ method focuses on the unmodified counterparts of phosphorylated peptides, which thus circumvents the ionization, fragmentation, and phospho-enrichment difficulties that hamper quantitation of stoichiometry in most common phosphoproteomics methods. Since iTRAQ enables multiplexing, simultaneous (phospho)proteome comparison between internal replicates and multiple samples is possible. The technique was validated on multiple instrument platforms by adding internal standards of high stoichiometry to a complex lysate of control and EGF-stimulated HeLa cells. To demonstrate the flexibility of Phospho-iTRAQ with regards to the experimental setup, the proteome coverage was extended through gel fractionation, while an internal replicate measurement created more stringent data analysis opportunities. The latest developments in MS instrumentation promise to further increase the resolution of the stoichiometric measurement of Phospho-iTRAQ in the future. The data have been deposited to the ProteomeXchange with identifier PXD001574.

journal_name

J Proteome Res

journal_title

Journal of proteome research

authors

Glibert P,Meert P,Van Steendam K,Van Nieuwerburgh F,De Coninck D,Martens L,Dhaenens M,Deforce D

doi

10.1021/pr500889v

subject

Has Abstract

pub_date

2015-02-06 00:00:00

pages

839-49

issue

2

eissn

1535-3893

issn

1535-3907

journal_volume

14

pub_type

杂志文章

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