Abstract：
:The final goal of the Russian part of the Chromosome-centric Human Proteome Project (C-HPP) was established as the analysis of the chromosome 18 (Chr 18) protein complement in plasma, liver tissue and HepG2 cells with the sensitivity of 10(-18) M. Using SRM, we have recently targeted 277 Chr 18 proteins in plasma, liver, and HepG2 cells. On the basis of the results of the survey, the SRM assays were drafted for 250 proteins: 41 proteins were found only in the liver tissue, 82 proteins were specifically detected in depleted plasma, and 127 proteins were mapped in both samples. The targeted analysis of HepG2 cells was carried out for 49 proteins; 41 of them were successfully registered using ordinary SRM and 5 additional proteins were registered using a combination of irreversible binding of proteins on CN-Br Sepharose 4B with SRM. Transcriptome profiling of HepG2 cells performed by RNAseq and RT-PCR has shown a significant correlation (r = 0.78) for 42 gene transcripts. A pilot affinity-based interactome analysis was performed for cytochrome b5 using analytical and preparative optical biosensor fishing followed by MS analysis of the fished proteins. All of the data on the proteome complement of the Chr 18 have been integrated into our gene-centric knowledgebase ( www.kb18.ru ).
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Zgoda VG,Kopylov AT,Tikhonova OV,Moisa AA,Pyndyk NV,Farafonova TE,Novikova SE,Lisitsa AV,Ponomarenko EA,Poverennaya EV,Radko SP,Khmeleva SA,Kurbatov LK,Filimonov AD,Bogolyubova NA,Ilgisonis EV,Chernobrovkin AL,Ivanov ASdoi
10.1021/pr300821nsubject
Has Abstractpub_date
2013-01-04 00:00:00pages
123-34issue
1eissn
1535-3893issn
1535-3907journal_volume
12pub_type
杂志文章abstract：:"Omics" studies reported to date have dealt with either thoroughly characterized single species or poorly explored meta-microbial communities. However, these techniques are capable of producing highly informative data for the analysis of interactions between two organisms. We examined the bacterial interaction between...
journal_title：Journal of proteome research
pub_type： 杂志文章
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journal_title：Journal of proteome research
pub_type： 杂志文章
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journal_title：Journal of proteome research
pub_type： 杂志文章
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2012-04-06 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr050298y
更新日期：2006-02-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr400274w
更新日期：2013-08-02 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.9b00334
更新日期：2019-11-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2012-02-03 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.6b00281
更新日期：2016-08-05 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr900008n
更新日期：2009-06-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr801048z
更新日期：2009-05-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.7b00703
更新日期：2018-03-02 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr9001614
更新日期：2009-07-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2018-12-07 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.5b00127
更新日期：2015-05-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.5b00255
更新日期：2015-11-06 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2006-03-01 00:00:00
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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更新日期：2009-03-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2011-07-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2009-04-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.7b00428
更新日期：2018-01-05 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2006-09-01 00:00:00
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journal_title：Journal of proteome research
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更新日期：2011-11-04 00:00:00
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journal_title：Journal of proteome research
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更新日期：2007-11-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章,评审
doi：10.1021/acs.jproteome.5b00857
更新日期：2016-01-04 00:00:00
